PURPOSE: Egg (egg white) allergies are among the most common food allergies in infants and young children. Serum egg white-specific IgE (sIgE) levels have been shown to be correlated with clinical symptoms, and the predictive decision point of sIgE levels has been proposed and used widely in the clinical setting. However, some patients whose sIgE levels to egg white are higher than the predictive decision point value show no clinical symptoms, and vice versa. This study was conducted to evaluate the clinical usefulness of sIgE antibodies to egg white and its components in the diagnosis of egg allergies. METHODS: Forty-one patients younger than 2 years of age with no experience of egg intake due to concerns regarding allergies or a non-specific clinical response to eggs were enrolled. Total IgE levels and the levels of IgE antibodies specific for egg white and its components (ovomucoid, ovalbumin, and conalbumin) were measured by ImmunoCAP testing. The clinical response of the subjects was confirmed by an open oral food challenge (OFC). RESULTS: Fifteen (71.4%) out of 21 patients in the egg white-sIgE > or =2 kU/L group showed a positive response, while 10 (50.0%) out of 20 patients in the egg white-sIgE <2 kU/L group showed a negative response to the OFC. There were no statistically significant differences in the levels of sIgE antibodies against egg white and its components between the positive and negative open OFC groups. In addition, there were no statistically significant differences in the levels of sIgE antibodies against egg white and its components based on an intra-group analysis. CONCLUSIONS: Our results show that the sensitivity and specificity of the predictive decision point values for egg white-sIgE antibodies by ImmunoCAP were relatively low in Korean children. In addition, no egg white component predicted the clinical reactivity of the subjects. We suggest that the predictive decision point value for a positive egg oral challenge test by ImmunoCAP should be re-evaluated. Moreover, we suggest that careful personal history recording and challenge tests are necessary for the correct diagnosis of an egg allergy.
Antibodies ; Child ; Egg Hypersensitivity ; Egg Proteins ; Egg White ; Eggs ; Food Hypersensitivity ; Humans ; Hypersensitivity ; Immunoglobulin E ; Infant ; Ovalbumin ; Ovum

BACKGROUND: The role of food allergy in children with atopic dermatitis (AD) has been emphasized. Eggs, cow's milk, soybeans are most common food allergens. We evaluated the serum specific IgE to egg whites (EW), cow's milk (CM), soybeans, and their clinical significance. METHODS: We performed a study with 1, 321 patients (male 739, female 582) who visited Samsung Medical Center during June 1998 and March 2002. We divided them into 9 groups by age. The serum total IgE and specific IgE to EW, CM, and soybeans were measured by Pharmacia AutoCAP system RAST (R) FEIA. RESULTS: The geometric mean of serum total IgE was increased with age (P< 0.05). EW (27.4%) was the most commonly sensitized food allergen in these subjects (P< 0.05), followed by CM (16.1%), and soybeans (8.1%). 37.2% of patients who are younger than 1-year-old showed positive reactions to EW. Reaction rate for this group was higher than others (P< 0.05). The positive reactions to EW were decreased with age (P< 0.05). But, the positive reactions to CM and soybeans did not show any relations to the age. The positive reactions to EW for 4-year-olds and younger were higher than those to CM and soybeans (P< 0.05). 30.7% of patients who had positive reactions to EW, especially 44.3% in age group 1-year-olds and younger (P< 0.05), showed higher specific IgE levels than diagnostic decision points of specific IgE to EW. CONCLUSION: The children with atopic dermatitis, especially young children, have high level of positive responses to egg whites, cow's milk. Sometimes they have serum specific IgE higher than diagnostic decision point. We think that young children with atopic dermatitis are more frequently related to egg and milk allergy.
Allergens ; Child* ; Child, Preschool ; Dermatitis, Atopic* ; Egg White* ; Eggs ; Female ; Food Hypersensitivity ; Humans ; Immunoglobulin E* ; Milk Hypersensitivity ; Milk* ; Ovum* ; Soybeans*

PURPOSE: The aim of this study was to evaluate the details of the clinical characteristics and food exposure types at the first symptom onset in immediate-type cow's milk allergy (CMA) and egg white allergy (EWA) in Korean children. METHODS: This study included children with immediate-type CMA (n=288) or EWA (n=233) with symptom onset time of 2 hours or less, who visited Samsung Medical Center, Ajou University Hospital, and Soonchunhyang University Seoul Hospital between September 2014 and August 2015. The details of clinical features and food exposure types at the first symptom onset were evaluated by retrospective medical record review using a standardized case report form. RESULTS: The median ages of first symptom onset were 10 months in CMA and 12 months in EWA. The most common types of exposure at the first symptom in CMA were formula milk (29.5%) and milk (29.5%), followed by cheese (17.7%) and yogurt (14.2%). The most common type of exposure in EWA was boiled eggs (35.6%), followed by rice/porridge/soup containing eggs (27.5%), pan-fried eggs (17.6%), and baked goods (9.9%). Cutaneous symptoms were most common in both CMA and EWA, and anaphylaxis was noticed in 36.1% and 30.3%, respectively. Baked goods containing milk or eggs also induced anaphylaxis. The symptom onset time was less than 30 minutes in the majority of patients and the most common place of occurrence was home in both CMA and EWA. CONCLUSION: This study provides comprehensive information on CMA and EWA, and therefore helps clinicians diagnose and guide appropriate food restriction in children with CMA and EWA.
Anaphylaxis ; Cheese ; Child* ; Egg Hypersensitivity ; Egg White* ; Eggs ; Humans ; Hypersensitivity* ; Hypersensitivity, Immediate ; Medical Records ; Milk Hypersensitivity ; Milk* ; Ovum* ; Retrospective Studies ; Seoul ; Yogurt

Food allergy is not uncommon among small children. Cow milk and eggs are most frequently incriminated as the major cause of food allergy. A 4-month-old female infant who did not have a previous history of contact with the egg developed anaphylactic shock when an emulsion of raw egg white was rubbed on the buttock by her mother to relieve erosive diaper dermatitis. She had been fed on breast milk. She had no past medical history of any other allergy and no family history of atopy, asthma or allergic rhinitis. Her IgE PRIST was 29.46 IU/ml and multiple antigen simultaneous testing chemiluminescent assay for food specific IgE antibody showed a level 4 positive value only to egg white.
Anaphylaxis* ; Asthma ; Breast* ; Buttocks ; Child ; Dermatitis ; Egg White* ; Eggs ; Female ; Food Hypersensitivity ; Humans ; Hypersensitivity ; Immunoglobulin E ; Infant* ; Luminescent Measurements ; Milk ; Milk, Human ; Mothers ; Ovum* ; Rhinitis ; Skin*

A 21 -year -old woman patient, from middle of February 2003, she had got fatigue, weight loss 5-6 kg within 3 weeks, bad appetite, indigestion. Then she had pain in right lower rib, 390C fever, cough, dry vomitting. She had got diagnosis of hepatic abscess and unsuccessfully treated by 10 days antibiotics. She had got cecal abscess and abscess block was removed. Stool examination found Fasciola hepatica eggs and ELISA serum test was (+) with F.gigantica antigen (collected from buffaloes and cows) by title of 1/12800, acidophil white blood cell 12%. Triclabendazole 10mg/kg gave good results.
Fasciola ; Vietnam ; Eggs ; Genome, Mitochondrial

We investigated whether maternal antibodies (mAbs) elicited by dams immunized with recombinant vaccine candidates against avian pathogenic Escherichia coli (APEC) can passively confer protective immunity to chicks. In the present study, pBP244 plasmids carrying selected antigens of APEC were transformed into Salmonella Typhimurium JOL912, which was used as a vaccine candidate against APEC. The hens were immunized with the vaccine candidates using prime or booster doses. The levels of IgG and sIgA specific to the selected antigens increased significantly following prime immunization. To evaluate the persistence of passively transferred mAbs, the levels of IgY and IgA were determined in egg yolks and whites, respectively. The eggs from the immunized group showed consistently increased levels of IgY and IgA until week 16 post-laying (PL) and week 8 PL, respectively, relative to the control group. The presence of mAbs was observed in chicks that hatched from the hens, and titers of plasma IgY were consistently raised in those from the immunized hens by day 14 post-hatching. Further, chicks from the immunized hens were protected from challenge with a virulent APEC strain, whereas those from non-immunized hens showed acute mortality.
Antibodies* ; Egg Yolk ; Eggs ; Escherichia coli* ; Escherichia* ; Immunization ; Immunization, Passive ; Immunoglobulin A ; Immunoglobulin A, Secretory ; Immunoglobulin G ; Mortality ; Ovum ; Plasma ; Plasmids ; Salmonella typhimurium ; Salmonella*

Tetrodotoxin is a neurotoxin, so gastrointestinal symptoms are very rare ; these described in the literature are merely nausea and vomiting. Severe complications in the gastrointestinal tract caused by tetrodotoxin have not been radiologically reported. US and CT show thickening of the gastric wall and contraction of the lumen, andupper gastrointestinal series show shortening, lobulation and irregularity of the lesser and greater curevature ofthe body and antrum similar to the findings of corrosive gastritis.
Barium ; Eggs* ; Gastritis* ; Gastrointestinal Tract ; Nausea ; Ovum* ; Tetraodontiformes* ; Tetrodotoxin ; Vomiting

The authors examined the Ascaris Eggs on the surface of the strawberries and in the soil of the strawberry yards. The results were as follows : 1. The number of Ascaris eggs detected from 870 strawberries grown on strawberry yards was 26, of which 17 eggs were found to be alive. 2. The mean number of Ascaris eggs detected in every 10gm of the soil of strawberry yards was 10.3. The Ascaris eggs were detected over 93% from th yards examined, which had been fertilized with both chemical fertilizer and night soil, or night soil only. 3. No Ascaris eggs was found from strawberries which were produced only with chemical fertilizer. 4. Ascaris eggs were detected 6 from 705 marketing strawberries studied, 3 of them developed to larval stage. 5. When the strawberries were washed by shaking 20 time after kept immersed in water for 10 minutes, the recovery rates of Ascaris eggs after first, 2nd, 3rd, 4th and 5th washing were 60, 87, 96, 99 ad 100%, respectively. 6. Besides Ascaris eggs of hook worm and Fasciolidae were also found from the strawberries examined.
Ascaris* ; Eggs* ; Fasciolidae ; Fragaria* ; Marketing ; Ovum* ; Soil* ; Water

The authors examined the Ascaris Eggs on the surface of the strawberries and in the soil of the strawberry yards. The results were as follows : 1. The number of Ascaris eggs detected from 870 strawberries grown on strawberry yards was 26, of which 17 eggs were found to be alive. 2. The mean number of Ascaris eggs detected in every 10gm of the soil of strawberry yards was 10.3. The Ascaris eggs were detected over 93% from th yards examined, which had been fertilized with both chemical fertilizer and night soil, or night soil only. 3. No Ascaris eggs was found from strawberries which were produced only with chemical fertilizer. 4. Ascaris eggs were detected 6 from 705 marketing strawberries studied, 3 of them developed to larval stage. 5. When the strawberries were washed by shaking 20 time after kept immersed in water for 10 minutes, the recovery rates of Ascaris eggs after first, 2nd, 3rd, 4th and 5th washing were 60, 87, 96, 99 ad 100%, respectively. 6. Besides Ascaris eggs of hook worm and Fasciolidae were also found from the strawberries examined.
Ascaris* ; Eggs* ; Fasciolidae ; Fragaria* ; Marketing ; Ovum* ; Soil* ; Water

Background: A/H5N1 influenza virus spreads from birds to humans and cause influenza diseases with high mortality rate. Vaccination is the most effective way to protect communities from pandemic, reduce morbidity and mortality. The study of creating A/H5N1 influenza vaccines in conformity with Vietnam was the urgent need. Institute of Vaccine\u2019s Achievement (IVAC) studied production of inactivated influenza vaccine for human on egg-grown from reassortants NIBRG-14. Objectives: In order to produce experimentally A/H5N1 influenza vaccine for human in accordance with WHO requirements and set up a viable process for production of the vaccines. Subjects and method: 10 days embryonated eggs and NIBRG-14 strains were served to the study with LAL method to check endotoxin, Kijehdal method to test total protein. Results: IVAC had produced successfully 5 lots of absorbed vaccine A/H5N1 (FLUVAC) using NIBRG-14 strains and embryonated eggs. Initially, production and quality control processes had been set up at IVAC by applying the recommendations of WHO. Conclusion: The success of the study was a basis of the approval of the government to establish a influenza vaccine manufacturing facilities.
Influenza A Virus ; H5N1 Subtype ; Influenza Vaccines ; Humans ; Eggs