OBJECTIVES: Although ionizing radiation is recognized as being harmful to humans, debate continues regarding the effects of low doses of ionizing radiation. Some studies have reported that low doses of ionizing radiation have a bio-positive effect, namely hormesis, and many researchers have attempted to find concrete and scientific evidence to prove this. To determine whether the discrepancy in effects of low doses of ionizing radiation arises from genetic differences, a study with a multicellular organism system such as fruit flies is needed. METHODS: After irradiation at a low-dose rate of gamma radiation with chronic (0.2 Gy) and acute (0.2, 0.75 and 4 Gy), the pupa rate, eclosion rate and life span were examined with various wild type strains of Drosophila melanogaster such as W1118, Oregon-R, and Canton-S. RESULTS: The life span of Oregon-R exposed to both acute and chronic, low-dose radiation (0.2 Gy) was increased. Although there was some difference in the longevity between the acute and chronic radiation rate, no other differences were found. In Canton-S, only acute dose (0.75 Gy) increased the life span, but it did not in W1118. CONCLUSIONS: Although there were some differences between wild type fruit fly strains in longevity, the low doses of ionizing radiation extended the life span of D. melanogaster. Further studies need to be carried out to explain the difference according to dose and dose rate of radiation in the tested strains.
Diptera ; Drosophila ; Drosophila melanogaster ; Fruit ; Gamma Rays ; Hormesis ; Humans ; Longevity ; Pupa ; Radiation, Ionizing

Freshly fed or half gravid An.sundaicus collected in An Thoi Dong commune, Can Gio district, Ho Chi Minh city were brought to the insectary of NIMPE for colonizing. Larvae were fed by a combination of 4gr bread powder + 4 shrimp powder + 2gr bean powder + 0.001gr vitamine B1. Larvae were contained at the conditions: a density of 0.3 larvae/cm2 of the surface water. Water for rearing was taken form the field with the salinity of 0.6 – 0.8%. Room temperature of 27 – 31oC, humidity of 60 – 90%. At these conditions, the image period took the time of 5 – 17 days. From the 8th generation (F8), mosquitoes were found to take free matting in the cage of 30 x 30 x 30 cm
Laboratories ; Culicidae ; diagnosis ; Diptera

No abstract available.
Diptera*

No abstract available.
Diptera*

No abstract available.
Diptera*

No abstract available.
Diptera

Background: Pomacea canaliculata (Lamarck, 1819) is an invasive freshwater snail in the Philippines that damages crops but is consumed as food. It is known to harbor parasites, some of which are pathogenic to humans. Objectives: The objective of this study is to examine P. canaliculata individuals present in Metro Manila for parasite infection and identify the parasites and other organisms associated with the snail using molecular identification. Methodology: P. canaliculata were collected from rivers and marketplaces in Metro Manila. Individuals were crushed and digested in Ash's digestive fluid and observed under a microscope. Collected parasites were subjected to DNA barcoding of the COI gene for putative identification. Results: A total of 462 snails were gathered from 15 sites, eight of which were market areas that sourced the snails from outside Metro Manila. No known parasites were found. Two snails were found to contain insect larvae in the mantle (0.43% infection). The closest BLAST matches for the two insect larvae were the chironomid fly Nilodorum tainanus (91.0% identity) from a snail in Sucat, Muntinlupa, and another chironomid Parachironomus sp. (92.8% identity) found in a snail originally from Cavite and brought to Calumpang, Marikina. Conclusion This study is the first report of the presence of chironomids in Philippine P. canaliculata. This could have an impact on the allergenic status of these mollusks if consumed while containing these chironomids. The absence of infection of other medically important parasites is possibly due to the patchy distribution of the snails and few interactions with the definitive hosts of known parasites.
Chironomidae ; Diptera

Blood fed An.dirus collected from Khanh Phu commune, Khanh Vinh district, Khanh Hoa province were reared and forced to lay eggs in the insectary of NIMPE. Parous females fed with mice blood were found to easy to lay eggs from 3-5 batches more. The conditions for image development of image stages were found to be as follows: temperature from 23 - 26oC, light time 12 – 24 hour with 90 – 150 Lux; the density of larvae is 1/5.7 cm2 rearing tool surface. Mosquitoes survived from 2 – 28 days. The temperature of 23 – 26oC and humidity of 70 –70 % were determined to be optimal for development of mosquitoes
Laboratories ; Biological Products ; Culicidae ; diagnosis ; Diptera

BACKGROUND: Tauopathies, a class of neurodegenerative diseases that includes Alzheimer's disease (AD), are characterized by the deposition of neurofibrillary tangles composed of hyperphosphorylated tau protein in the human brain. As abnormal alterations in histone acetylation and methylation show a cause and effect relationship with AD, we investigated the role of several Jumonji domain-containing histone demethylase (JHDM) genes, which have yet to be studied in AD pathology. METHODS: To examine alterations of several JHDM genes in AD pathology, we performed bioinformatics analyses of JHDM gene expression profiles in brain tissue samples from deceased AD patients. Furthermore, to investigate the possible relationship between alterations in JHDM gene expression profiles and AD pathology in vivo, we examined whether tissue-specific downregulation of JHDM Drosophila homologs (kdm) can affect tauR406W-induced neurotoxicity using transgenic flies containing the UAS-Gal4 binary system. RESULTS: The expression levels of JHDM1A, JHDM2A/2B, and JHDM3A/3B were significantly higher in postmortem brain tissue from patients with AD than from non-demented controls, whereas JHDM1B mRNA levels were downregulated in the brains of patients with AD. Using transgenic flies, we revealed that knockdown of kdm2 (homolog to human JHDM1), kdm3 (homolog to human JHDM2), kdm4a (homolog to human JHDM3A), or kdm4b (homolog to human JHDM3B) genes in the eye ameliorated the tauR406W-engendered defects, resulting in less severe phenotypes. However, kdm4a knockdown in the central nervous system uniquely ameliorated tauR406W-induced locomotion defects by restoring heterochromatin. CONCLUSION: Our results suggest that downregulation of kdm4a expression may be a potential therapeutic target in AD.
Acetylation ; Alzheimer Disease ; Brain ; Central Nervous System ; Computational Biology ; Diptera ; Down-Regulation ; Drosophila melanogaster ; Drosophila ; Heterochromatin ; Histones ; Humans ; Locomotion ; Methylation ; Neurodegenerative Diseases ; Neurofibrillary Tangles ; Pathology ; Phenotype ; RNA, Messenger ; tau Proteins ; Tauopathies ; Transcriptome

BACKGROUND/OBJECTIVES: The aim of this study was to evaluate the biological and sleep-promoting effects of combined γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) using caffeine-induced sleepless fruit flies, ICR mice, and Sprague-Dawley rats. MATERIALS/METHODS: Video-tracking analysis was applied to investigate behavioral changes of Drosophila melanogaster. Pentobarbital-induced sleep test and electroencephalogram (EEG) patterns were used for analysis of sleep latency, duration, and quantity and quality of sleep in vertebrate models. RESULTS: Administration of combined GABA/5-HTP could significantly reverse the caffeine induced total distance of flies (P < 0.001). Also, individually administered and combined GABA/5-HTP significantly increased the total sleeping time in the caffeine-induced sleepless ICR mice (P < 0.001). In the caffeine-induced sleepless SD-rats, combined GABA/5-HTP showed significant differences in sleep quality between individual amino acid administrations (P < 0.05). CONCLUSIONS: Taken together, we identified inhibitory effects of combined GABA/5-HTP in locomotor activity, sleep quantity and quality in caffeine-induced sleepless models, indicating that combined GABA/5-HTP may be effective in patients with insomnia by providing sufficient sleep.
5-Hydroxytryptophan ; Amino Acids* ; Animals ; Caffeine ; Diptera ; Drosophila melanogaster ; Electroencephalography ; Fruit ; gamma-Aminobutyric Acid ; Humans ; Mice ; Mice, Inbred ICR ; Motor Activity ; Rats, Sprague-Dawley ; Sleep Initiation and Maintenance Disorders ; Vertebrates