1.Evaluation of the effectiveness of solfac WP10 and solfac EW050 in malaria mosquito control in Viet Nam
Journal of Malaria and parasite diseases Control 2003;0(4):55-60
To evaluate the effectiveness in malaria vector control of residual spraying with Solfac WP10 at dose of 50mg ai/m2 and bednet impregnated with Solfac EW050 at a dose of 50mg ai/m2, based on bioassays, side effects. The results showed that: in pilot doses, the residual effect with Solfac 10WP produced the residual effect for 6 months on the walls made of wood with the bioassay mortality of 70.60%; for 2 months on the wall made of brick with the bioassay mortality of 61.63%. The residual effect of Solfact EW 050 on polyester bednet is 4 months after 2 washes, with the bioassay mortality of 73.6%. House spraying with Solfac WP10 and bednet impregnation with Solfac EW 050 at a dose of 50mg/ ai/m2 to prevent malaria due to mosquito were found to cause light side effects
malaria
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Antimalarials
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disease
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Culicidae
2.Rapid diagnosis of P.falciparum malaria using “paracheck P.F.” technique: observation of 127 cases
Journal of Preventive Medicine 2002;12(2):59-62
Testing in 127 patients with malaria at the Center of Malariological- Parasitological - Entomological prevention and control and hospitals in A Luoi, Nam Dong, Phu Loc, Huong Thuy, Huong Tra, Phong Dien, Phu Vang districts and Hue city with 2 types: Giemsa staining and Parachek P.F technique. Results showed that: 40 positive samples with P.fal and 87 negative samples. Results of the 2 methods were similar
Malaria
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Antimalarials
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diagnosis
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Malaria, Falciparum
3.Study on synthesis of polymeric drug: Synthesis of antimalarial drug with delayed effect and natural origin
Pharmaceutical Journal 1999;282(10):9-11
The coupling procedure of antimalarial agent (SR2) onto polymeric carrier (SR1) by covalent bond (amide linkage) was studied. Some factors of drug coupling reaction was investigated.
Antimalarials
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Pharmaceutical Preparations
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drug therapy
4.Pharmacokinetics of the antimalarial combination CV8 when given in the manner of a therapeutic dosage regimen in healthy male subjects
Journal of Malaria and parasite diseases Control 2003;3():48-54
The study was conducted in 2002 for analysis by non-linear regression of compartmental pharmacokinetic model. The results: the plasma-concentration of piperaquine at day 1 and day 3 after CV8 administration were determined. At first stage, only the basic parameters derived from non-compartmental methods were presented and another parameters, models is expected to be presented in later stages. The plasma-concentration of piperaquine of all volunteers were determined with Cmax on day 1 was 1015 nM/L, on day 2 was 1050 nM/L, T1/2 was 30-57 days and Tmax of piperaquine was 3-5 hours after using
Malaria
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Antimalarials
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Pharmacokinetics
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Therapeutics
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drugs
5.In vitro response of Plasmodium falciparum to some medicinal plants in Vietnam
Pharmaceutical Journal 1999;282(10):13-17
The response of the malarial parasite, Plasmodium falciparum to a number of Vietnamese medicinal plants in vitro was carried out. The results showed that the ethanol extract of the plant materials has a stronger inhibitory effect on the growth of P. faciparum than the corresponding water extract. Both ethanol and water extracts of the leaves of Dichroa febrifuge Lour. and the ethnologic extracts from the leaves of Parthenium hysterrophorus L. and Harrisonia perforate (Blanco) Merr. have a clear effect on the chloroquine sensitivity as well as the chloroquine resistant P. falciparum strain
Plants, Medicinal
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Antimalarials
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Plasmodium falciparum
6.Studies on the efficiency of chloroquine and chloroguanide as antimalarials: I. as suppressants.
SMITH HF ; DY FJ ; CABRERA DF
Acta Medica Philippina 0000;():0-
Chloroquine (Aralen) and chloroguanide (Paludrine) were tried as suppressants in two different malarious localities in the Philippines for 39 to 40 weeks. The drugs were given under strict supervision in single weekly doses to two groups of subjects and those who developed overt malaria were given therapeutic doses of the respective drugs. Control groups maintained in both localities received sodium bicarbonate as a placebo and those exhibiting overt attacks were treated with quinine sulfate. Test and control subjects who had overt malaria were continued on their respective weekly drugs after a course of treatment as mentioned above. Intensive vector control measures were instituted within and around the localities starting two months before the cessation of suppressive treatment in order to stop transmission, and the number of subjects developing malaria thereafter was recorded for at least 14 weeksThe following observations were made: 1. Among 125 subjects given 0.50 Gm. chloroquine diphosphate once weekly for 39 to 40 weeks, 13 cases were suppressed, but 2 came down with overt malaria during the period of suppressive treatment2. Among 104 subjects given 0.20 Gm. chlorguanide once weekly for 39 to 40 weeks, 13 cases were suppressed, but 17 came down with overt malaria during the period of suppressive treatment3. Among 106 subjects given 0.60 Gm. sodium bicarbonate as a placebo once weekly for 39 to 40 weeks, 54 developed overt malaria4. Eight subjects with P. falciparum either at the onset or during the period of suppression with chloroquine did not exhibit gametocytes at any of the weekly examinations conducted during the 39 weeks of suppressive treatment. Chlorguanide appeared to be ineffective against vivax and falciparum gametocytes5. Among 125 subjects given 0.50 Gm. chloroquine diphosphate once weekly for 39 to 40 weeks, 2 had nausea and vomiting, 1 had vertigo, and 1 had flatulence and the desire to stool soon after taking the drug. These untoward effects were mild, temporary, and did not require withdrawal of the drugIt was shown that with the dosage regimen employed, the suppressive activity of chloroquine was greater than that of chlorguanide. It is possible, however, that with increased dosage chlorguanide may prove to be an efficient suppressantThe response of the infections to chloroquine and chlorguanide, particularly to the latter, was not the same in the two localities, suggesting the presence of different strains of plasmodia in the country. The possibility of the introduction of foreign strains into the Philippines through seeded American and Japanese soldiers during World War II is advanced. (Author)
Plasmodium, Chloroguanide, Chloroquine, Antimalarials, Placebos
7.Radomized clinical trials of dihydroartemisisnin - piperaquine against multi-drug resistant falciparum malaria in Viet Nam
Journal of Malaria and parasite diseases Control 2003;0(4):47-54
A hospital based pilot study, conducted in a Tropical diseases hospital in Ho Chi Minh City of Viet Nam, compared the efficacy of three day regimens of dihydroartemisinin-trimethoprim-piperaquine (DTP total dose 4.7/13/47 mg/kg) with the standard antimalarial regimen in Vietnam, artersunate-mefloquine (A3M total dose 12/25 mg/kg) in non immune patients with uncomplicated falciparum malaria. 114 patients were enrolled (78 to the DTP arm and 36 to the A3M arm). The subsequent open randomised trial conducted at a Provincial Health Station compared DTP, dihydroartemisinin-piperaquine (DP) and A3M in 400 patients. In both studies all patients received derectly observed therapy and were followed for 56 days. The results: In the pilot study, the 56 day cure rate, adjusted for reinfections by PCR genotyping was 97.4% in the DTP group and 100% in the A3M group. In the second study, the cure rate were similar in each group. The DTP regimens were well tolerated, less than 3% of patients experienced possible drug related side effects compared with 16% of A3M patients
Malaria
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Antimalarials
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Malaria, Falciparum
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artemisisnins
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drugs
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therapeutics
8.Method for rapid synchronization of different growth cycles of Plasmodium falciparum in vitro and application in differential gene expression profile of 3D7 after dihydroartemisinin treatment.
Zhong-Yuan ZHENG ; Li-Na CHEN ; Ting YANG ; Hui LIU ; Shui-Qing QU ; Yuan-Min YANG ; Yu-Jie LI ; Shu-Qiu ZHANG
China Journal of Chinese Materia Medica 2020;45(10):2454-2463
Plasmodium culture in vitro is often used as an antimalarial drug evaluation model, but the lifecycle of P. falciparum culture in vitro tends to be disordered, which affects the research and evaluation of antimalarial drug mechanism in vitro. By combining magnetic bead separation method with sorbitol synchronization method, a synchronization method was constructed to quickly acquire different lifecycles of P. falciparum and obtain large amounts of parasite with a narrow synchronization window in a short period. Furthermore, the dihydroartemisinin(DHA) was used to treat the early trophozoite phase of P. falciparum 3 D7 for 4 h. Then mRNA was extracted and RNA-seq was conducted to analyze the differential expression of mRNA after drug treatment and obtain the differential gene expression profile. Differential expression of up-regulated genes and down-regulated genes was analyzed according to the screening criteria of |log_2FC|>1 and P<0.05. There, 262 genes were up-regulated and 77 genes were down-regulated. GO functional enrichment analysis of all the differentially expressed genes showed that the enrichment items mainly included cell membrane components, transporter activity, serine/threonine kinase activity, Maurer's clefts(MCs), rhoptry, antigen variation and immune evasion. The enrichment of KEGG pathway included malaria, fatty acid metabolism and peroxisome. Protein-protein interaction(PPI) analysis showed that the down-regulated genes in the modules with high degree of association included rhoptry, myosin complex, transporter and other genes related to the important life activities of malaria invasion and immune escape; the up-regulated genes were mainly related to various toxic exportins of malaria, such as PfSBP1 of MCs. qRT-PCR was used to verify the expression level of some genes, and most of the results were the same as the sequencing results. SBP1 was significantly up-regulated, while some antigenic protein expression levels were down-regulated. Above all, key molecules of DHA therapy were mainly involved in the parasites' rhoptry, transporter, antigenic variation, plasmodium exportin. These results offer us many hints to guide the further studies on mechanism of artemisinin and provide a new way for development of new antimalarial drugs.
Animals
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Antimalarials
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Artemisinins
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Erythrocytes
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Plasmodium falciparum
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Transcriptome
9.Research progress on chemical constituents and pharmacological effects of Ajania plants.
Min YAO ; Xin-Jun DI ; Zhi-Xian JING ; Jun-Mao LI ; Zhi-Qiang LI ; Ming-Ming YUAN ; Ren CI ; Yu-Lin FENG ; Shi-Lin YANG
China Journal of Chinese Materia Medica 2023;48(11):2904-2918
Ajania belonging to the subtribe Artemisiinae of Anthemideae(Asteraceae) is a genus of semi-shrubs closely related to Chrysanthemum. There are 24 species of Ajania in northwestern China, most of which are folk herbal medicines with strong stress tolerance. Modern medical studies have demonstrated that the chemical constituents of Ajania mainly include terpenoids, flavonoids, phenylpropanoids, alkynes, and essential oils. These compounds endow the plants with antimicrobial, anti-inflammatory, antitumor, antimalarial, antioxidant, and insecticide effects. In this study, we reviewed the research progress in the chemical constituents and pharmacological activities of Ajania, aiming to provide reference for the further research and development of Ajania.
Asteraceae
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Chrysanthemum
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Alkynes
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Antimalarials
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Antioxidants/pharmacology*
10.Halofantrine in the treatment of uncomplicated falciparum malaria with a three-dose regimen in Papua New Guinea: a preliminary report
F. W. Hombhanje ; R. K. Kereu ; P. Bulungol ; R. Paika
Papua New Guinea medical journal 1998;41(1):23-29
We evaluated the efficacy and safety of halofantrine in 19 patients with acute uncomplicated falciparum malaria. Each patient received oral halofantrine hydrochloride 500 mg every 6 hours for 3 doses (total 1.5 g). In almost all the patients clinical symptoms of malaria and parasitaemia disappeared within 2 and 3 days, respectively, of starting treatment. We observed no recurrence of parasitaemia during 14 days of follow-up. Tolerance to halofantrine was good except for minor and self-limiting gastrointestinal side-effects. Haematological and biochemical indices were not seriously affected. Halofantrine-induced prolongation of Q-T/Q-Tc intervals was the consistent cardiac manifestation in 84% of patients. The Q-T/Q-Tc interval prolongation increased with each dose; it reached a maximum between 18 and 24 hours and thereafter returned to baseline. These preliminary data indicate that, apart from the cardiac side-effects, halofantrine is an effective and safe drug, well tolerated by most of the patients in the study.
PIP: The authors evaluated the efficacy and safety of halofantrine in 19 patients with acute uncomplicated falciparum malaria. Each patient received oral halofantrine hydrochloride 500 mg every 6 hours, for a total of 3 doses (total 1.5 g). In almost all the patients clinical symptoms of malaria and parasitemia disappeared within 2 and 3 days, respectively, of starting treatment. They observed no recurrence of parasitemia during 14 days of follow-up. Tolerance to halofantrine was good except for minor and self-limiting gastrointestinal side effects. Hematological and biochemical indices were not seriously affected. Halofantrine-induced prolongation of Q-T/Q-Tc intervals was the consistent cardiac manifestation in 84% of patients. The Q-T/Q-Tc interval prolongation increased with each dose; it reached a maximum between 18 and 24 hours and thereafter returned to baseline. These preliminary data indicate that, apart from the cardiac side effects, halofantrine is an effective and safe drug, well tolerated by most of the patients in the study.
Antimalarials - administration &
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dosage
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Antimalarials - therapeutic use
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Electrocardiography
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Malaria, Falciparum - blood