1.Progress in researches on L-asparaginase targeted to childhood leukemia.
Yan-qin CUI ; Fu-xiong CHEN ; Zi-liang WU
Chinese Journal of Pediatrics 2004;42(3):227-230
Antineoplastic Agents
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administration & dosage
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adverse effects
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therapeutic use
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Asparaginase
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administration & dosage
;
adverse effects
;
therapeutic use
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Child
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Drug Administration Routes
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Drug Administration Schedule
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Forecasting
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Humans
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Leukemia
;
drug therapy
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
;
drug therapy
2.Allele-specific PCR and its application in forensic science.
Yan-chai NIE ; Bin WANG ; Zi-qin ZHAO ; Huai-gu ZHOU
Journal of Forensic Medicine 2014;30(4):282-287
Allele-specific polymerase chain reaction (AS-PCR) is a technique based on allele-specific primers, which can be used to analyze single nucleotide polymorphism (SNP) effectively including the transition, transversion and insertion/deletion polymorphism and has been exploited in the study of diseases research, molecular diagnosis, and forensic biological evidence. The article systematically reviews the principle, the detection methods, improvement of AS-PCR, and its research updates in the fields of autosome, Y chromosome and mitochondrial SNP, as well as its application in forensic science.
Alleles
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DNA Primers
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Forensic Sciences
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Humans
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Polymerase Chain Reaction
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Polymorphism, Genetic
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Polymorphism, Single Nucleotide
3.The alternative splicing & expression of fibronectin IIIcs segment and its relationship with wound healing.
Journal of Forensic Medicine 2001;17(4):245-248
Fibronectin is an important large adhesive glycoprotein of the extracellular matrix, which is alternatively spliced in three regions, designated EIIIA, EIIIB and IIIcs respectively. IIIcs contains two binding domains for a variety of cell surface and extracellular ligands. Through this multiplicity of adhesive activities, IIIcs can fulfill key roles in a broad spectrum of physiological processes, such as cell spreading and migration, differentiation and embryogenesis, wound healing, malignant transformation and metastasis, etc. Here, we will discuss the structure, biological property, and function of IIIcs splicing variants and its forensic applications.
Alternative Splicing
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Animals
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Cattle
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Fibronectins/physiology*
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Forensic Medicine
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Humans
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Rats
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Wound Healing/physiology*
4.MicroRNAs: important mediators of ossification.
Zi-qiang WANG ; Yan-qin LU ; Jin-xiang HAN
Chinese Medical Journal 2012;125(22):4111-4116
5.The expression of FN-IIIcs in the wound healing and its relationship with injury time.
Yan JI ; Zi-qin ZHAO ; Yun-ju GU
Journal of Forensic Medicine 2002;18(1):4-6
OBJECTIVE:
To research the expression of alternative splicing segment of fibronectin-IIIcs in incised wound of skin, to offer the experimental data for early injury time judgement.
METHODS:
Using in situ hybridization with DIG-labeled anti-sense RNA probe, the expression of FN-IIIcs domain was detected in both human and rat skin incised wound.
RESULTS:
(1) The expression of FN-IIIcs improved from the time of 30 min after injury, reached peak at 6 h and then reduced gradually. (2) The positive expression cells were mainly distributed in hair follicles, sebaceous glands and endothelium, however, in human beings they were basement cells of epidermis.
CONCLUSION
The expression of FN-IIIcs domain would be a supposed useful criteria for early injury time judgement.
Animals
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Fibronectins/genetics*
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Forensic Medicine
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Humans
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Male
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RNA, Messenger/biosynthesis*
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Rats
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Rats, Sprague-Dawley
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Skin/metabolism*
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Time Factors
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Wound Healing/physiology*
6.Experimental study on effect of arsenic trioxide on vascular smooth muscle cells
Qin LU ; Yan-Li AN ; Zi-Yu WANG ; Dong-Sheng ZHANG ; Huan-Zhang NIU ; Juan-Juan FANG ; Gao-Jun TENG ;
Chinese Journal of Radiology 2001;0(04):-
Objective To investigate the effect of arsenic trioxide(As_2 O_3)nanoparticles on rabbit vascular smooth muscle cells in vitro in comparison with normal form As_2 03.Methods The rabbit vascular smooth muscle cells were cultured in vitro.Nano and normal forms of As_2O_3 with drug concentrations of 3?mol/L were added into the cells.Cell proliferation curve was drawn according to the light absorption values of MTT test.Flow cytometry was applied to observe the apoptosis.DNA was extracted and underwent electrophoresis.Results Cell proliferation treated with the 3?mol/L concentration of As_2O_3 was inhibited. Cell growth was inhibited markedly with increased treatment time,and the inhibition effect of nano drug form seemed stronger than that of normal form.MTT light absorption values of cells treated at 24,48 and 72 h showed statistically significant difference(H=10.934,15.039,15.539,P
7.Effects of Transcranial Direct Current Stimulation on Motor Function of Upper Limbs in Stroke Patients
Yu YIN ; Xiu-qin ZUO ; Yan-ling LÜ ; Zi-shan JIA ; Zhen-biao ZHAO ; Ya-ping HUAI ; Yan-ning YAN
Chinese Journal of Rehabilitation Theory and Practice 2015;21(7):830-833
Objective To investigate the effects of transcranial direct current stimulation (tDCS) on motor function of upper limbs of stroke patients. Methods 80 stroke patients were randomly divided into experimental group and control group. Both groups accepted routine rehabilitation, while the experimental group accepted anodal stimulation, and the control group received sham stimulation. They were assessed with Brunnstrom stages of arms and hands, Fugl-Meyer Assessment (FMA) of upper extremities, Action Research Arm Test (ARAT), Motor Assessment Scale (MAS) and modified Barthel Index (MBI) before and 1 month after treatment. Results All the scores improved in both groups after treatment (P<0.05), and improved more in the Brunnstrom stages of arms and hands, FMA, ARAT in the experimental group than in the control group (P<0.05). Conclusion tDCS may promote the recovery of arms and hands function of stroke patients.
8.Fos expression in rat skin after different incised wound healing interval.
Ning Guo LIU ; Zi Qin ZHAO ; Yun Ju GU ; Yi Jiu CHEN ; Zu Kang YAN ; Yan Ping LIAO
Journal of Forensic Medicine 2001;17(4):196-197
OBJECTIVE:
To investigate the expression of c-fos in rats' skin during wound healing.
METHODS:
Immunohistochemistry was conducted on paraffin section from incised wounding model of rat skin.
RESULTS:
Fos protein improved from the time of 10 min after wounding in the wound edge, then it reached peak at 3 h. 24 h after injury, the quantity of Fos expression had no difference with that of normal skin.
CONCLUSION
Fos is sensitive after wound, but should be used with other criteria in wounding interval estimation as it's unstediness.
Animals
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Genes, Immediate-Early
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Immunohistochemistry
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Male
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Proto-Oncogene Proteins c-fos/biosynthesis*
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Rats
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Rats, Sprague-Dawley
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Skin/metabolism*
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Time Factors
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Wounds and Injuries/metabolism*
9.A survey on mental health among traffic policemen in Tianjin.
Feng-shi TIAN ; Jun YAN ; Jia PENG ; Yan WU ; Zi-qin ZHAO ; Song YUE
Chinese Journal of Epidemiology 2012;33(10):1095-1096
Adult
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China
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epidemiology
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Female
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Humans
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Male
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Mental Health
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Middle Aged
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Police
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Young Adult
10.The regulation of ovarian reserve function by Zuogui pills combined with cetrorelix
Qing-yu LI ; Jing-jing GAO ; Yan-jin FU ; Meng-sha LONG ; Yi-yao ZHANG ; Zu-yu MENG ; Shao-zi LIN ; Jia-jia QIN
Acta Pharmaceutica Sinica 2022;57(7):2108-2114
The purpose of this study was to investigate how Zuogui pills from the Kidney-tonifying and Nourishing Yin formula, in combination with the gonadotrophin-releasing hormone antagonist cetrorelix, affected the ovarian local oxidative stress response in decreasing ovarian reserve (DOR) mice. All animal experiments were carried out in accordance with the guidelines and standards established by Jinan University's Experimental Animal Management Committee. Cyclophosphamide (CTX)-treated DOR mice were given Zuogui pills, cetrorelix, or a combination of the two drugs intragastrically. After treatment, there were changes in the estrous cycle, serum sex hormone levels, oxidative stress-related indexes, growth biochemical factor levels, and SIRT1/P53/P21 expression. In comparison to the model group, the Zuogui pills and the cetrorelix+Zuogui pills group had significantly prolonged estrous periods and shortened interestrous periods, and the cetrorelix+Zuogui pills group had a significantly shortened cycle length. Follicle-stimulating hormone (FSH) decreased and estradiol (E2) increased in all treatment groups compared to the model group, oxidative stress indexes nitric oxide synthase (NOS), nitric oxide (NO), and reactive oxygen species (ROS) decreased, growth biochemical factors brain derived neurotrophic factor (BDNF) and growth differentiation factor 9 (GDF-9) concentrations increased significantly, and leukemia inhibitory factor (LIF) showed no significant change. SIRT1/P53/P21 immunohistochemical results revealed that, when compared to the model group, the expression of SIRT1 increased while the expression of P53 and P21 proteins decreased in all treatment groups, with the cetrorelix+Zuogui pills group having the largest decrease, with significant differences in all indicators. We conclude that cetrorelix combined with Zuogui pills for kidney nourishing and Yin recipe improved the oxidative stress response in the follicle by regulating the SIRT1/P53/P21 pathway, reducing peroxide product production, protecting ovarian function, and regulating ovarian hormone secretion, and its efficacy is superior to that of cetrorelix or Zuogui pills alone.