The animal experiments,clinical researches and epidemiological investigations indicated that genistein has the effect for prevention and treatment of osteoporosis,cardiovascular disease and cancer.Genistein can inhibit cellular and humoral immunity and can regulate cell apoptosis.The chemical structure,biological effects and the mechanism of genistein attracted much attention.The current research advance on genistein was summarized and the prospect on the theoretical significance,clinical importance was presented in this paper.

Hypothyroidism is one of the most common endocrine disorders in childbearing or pregnant women.Gestational hypothyroidism can increase the incidence rate of the variety of pregnancy complications and adverse pregnancy outcome.It also can make adverse effects on the fetus and neonate,such as death,placental abruption,fetal malformation,intrauterine growth retardation and preterm,low birth weight,neonatal respiratory distress syndrome,it can also cause abnormal thyroid function of neonate,severely can cause nerve,mental,physical and mental developmental abnormalities and even death.Therefore,it is recommended that childbearing women who are at high risk for pregnancy should strengthen to monitor pregnancy thyroid function and treat as soon as possible in order to reduce the adverse effects on offspring.

OBJECTIVE: To evaluate the current situation and trend of narcotic drug use in our hospital where the authors work in order to provide the reference for scientific management and rational use of these drugs. METHODS: The yearly amount of narcotic drugs administered in the whole hospital, the yearly amount in the separate departments, as well as drug expenditures and ratios between January 2002 and November 2005 were added up and analyzed statistically. RESULTS: The amount of bucinnazine use dominated in the first. The amount of morphine for oral use was increasing year by year. The amount of fentangl transdernal patch use was also bigger and increasing rapidly. However, the amount of pethidine and morphine for injection use was decreasing. CONCLUSION:Analgetics for oral and transdermal use will be the main categories of analgetics in the future.

Objective To investigate effectiveness of dexmedetomidine on preventing shivering of patients after spinal surgery.Methods A total of 78 cases with spinal surgery under anesthesia were randomly divided into study group(dexmedetomidine treatment) and the control group(saline treatment).Heart rate(HR)and mean arterial pressure were observed and recorded at 15,30,60 min after intubation,before and after extubation.Ramsay sedation score,chills grading,chills occur time and postoperative adverse reactions were recorded.Results In study group,HR were ((72.3 ± 5.2) time/min,(83.2 ± 4.5) time/min),mean arterial pressure were((72.7 ±5.0) mmHg,(90.3 ±7.9) mmHg) at before and after extubation,significant lower than that of control group((73.6 ±4.9) time/min,(88.9 ±4.9) time/min; (78.5 ±4.3) mmHg,(95.5 ± 14.9)mmHg;P＜0.05).The incidence of shivering (7.7％) in study group was significantly lower than the control group(30.8 ％,x2 =6.685,P =0.009).Ramsay sedation score at immediately,30 min,60 min into the PACU were (3.1 ± 1.0),(2.1 ± 0.4),(2.0 ± 0.3) respectively,higher than those of control group ((2.0 ± 0.4),(1.3±0.2),(1.1 ±0.3) ; F inter-group =12.543,P＜0.001;F inner-group =9.391,P ＜0.001;F interaction =5.972,P =0.023).The incidence of adverse reactions such as nausea and vomiting in the control group was higher than those of the study group(P =0.003),while the incidence of dry mouth in study group was higher (P =0.002).Conclusion Intraoperative dexmedetomidine can significantly reduce the incidence and degree of shivering in patients with orthopedic spinal surgery after general anesthesia.Dexmedetomidine is proved to be a more ideal drug to prevent shivering in orthopedic spine surgery after general anesthesia.

Objective To study the effect of 3％ hypertonic saline(HS) and 20％ mannitol on cerebral oxygen metabolism in patients undergoing neurosurgery for supratentorial glioma.Methods 56 patients undergoing neurosurgery for supratentorial glioma were evenly divided into two groups.Group A(n =28) was treated by 3％ hypertonic saline(5.35ml/kg).Group B (n =28) was treated by 20％ mannitol(1 g/kg).CSFP was tested before hypertonic saline or mannitol infusion (T0),immediately (T1),15,30,60,120min after infusion (T2 ～5).Various blood-gas indexes were observed at To,T3 ～5.CaO2,CjvO2,Da-jvO2 and CERO2 were calculated.Results CSFP significantly dropped from T2 to T5 in both groups (P ＜ 0.05).Da-jvO2 and CERO2 were significantly reduced at T4～5 in both groups (P ＜ 0.05).There was no significant difference between the two groups.Conclusion 3 ％ HS is as efficient as 20％ mannitol in reducing intracranial pressure and improving cerebral oxygen metabolism in patients undergoing neurosurgery for supratentorial glioma.

Objective To determine the brain protective effects of inhalation anesthetic pretreatment in infants undergoing open-heart surgery under extracorporeal circulation (ECC).Methods Forty-five infants (no more than 3 years old) with congenital heart diseases who received surgical treatment were divided into 3 groups by table of random digit,sevoflurane pretreatment group (group S,15 cases),isoflurane pretreatment group (group Ⅰ,15 cases) and control group (group C,15 cases).In pretreatment group,all infants had been inhaled 1.0-1.5 MAC sevoflurane or isoflurane continually for over 60 min after intubation,while group C did not receive pretreatment.Blood samples were taken before (T1) and in 5 min after ECC (T2),occlusion of aorta (T3),30 min after ECC (T4),end ofECC (T4),and 6 and 24 h after the end of ECC (T6 and T7).Serum levels of S 100 β protein and neurone specific enolase (NSE) were measured by enzyme-linked immunosorbent assay.Results There was no significant difference in the serum levels of S100β protein and NSE among the 3 groups in T1 (P＞0.05).There was no significant difference in the serum levels of S100β protein and NSE in group S and group Ⅰ between T1 and T2-7 (P＞ 0.05).There was no significant difference in the serum levels of S100 β protein and NSE in T2-7 between group S and group Ⅰ (P ＞ 0.05).In group C,there were significant differences in the serum levels of S100 β protein in T4-6 and NSE in T4-7 than those in T1 (P＜ 0.05).In T4-6,the serum levels of S100 β protein and NSE in group S and group Ⅰ were significantly lower than those in group C [S100 β protein:(0.45 ± 0.31),(0.47 ± 0.35) μ g/L vs.(0.73 ± 0.31) μ g/L,(0.53 ± 0.33),(0.54 ± 0.36) μ g/L vs.(0.91 ± 0.30) μ g/L,(0.47 ± 0.34),(0.49 ±0.35) μ g/L vs.(0.79 ± 0.40) μ g/L,P ＜ 0.05； NSE:(3.26 ± 1.34),(3.32 ± 1.34) μ g/L vs.(7.20 ± 2.71)μ g/L,(5.57 ± 1.49),(5.61 ± 1.52) μ g/L vs.(7.68 ± 3.68) μ g/L,(2.29 ± 1.21),(2.34 ± 1.22) μ g/L vs.(9.46 ± 4.61) μg/L,P ＜ 0.05].Conclusion Sevoflurane or isoflurane pretreatment significantly decreases the serum levels of S100 β protein and NSE during open-heart operation in infants with ECC.

Objective To investigate the correlation between serum procalcitonin (PCT) and acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) score and prognosis in acute exacerbation of chronic obstructive pulmonary disease (AECOPD).Methods A total of 88 patients with AECOPD admitted to emergency department of Peking university third hospital were prospectively studied,and were divided into three groups,namely high score group,median score group and low score group according to APACHE Ⅱ score.Serum PCT,hypersensitive C-reactive protein (hs-CRP),WBC and lactate were assayed within the first 24 hours after admission,the differences in those indicators between three groups were analyzed; and the correlation between PCT and APACHE Ⅱscore,hs-CRP,WBC,lactate were investigated.The patients were divided into survival group and death group according to prognosis,and the differences in those indicators between the two groups were analyzed.Results The serum levels of PCT was higher in high score group (0.60±0.32) ng/ml than that in median score group (0.36 ±0.23) ng/ml and that in low score group (0.24 ±0.19) ng/ml,differences between groups were statistically significant (P ＜0.01).The hs-CRP was higher in high score group M (P25,P75) 36.88 (10.14,47.16) mg/L than that in median score group 15.00 (3.64,30.33) mg/L and that in low score group 14.77 (4.35,15.80) mg/L (P =0.046).The PCT significantly correlated with APACHE Ⅱ and hs-CRP (P ＜0.01).The serum levels of PCT,APACHE Ⅱ score,hs-CRP and lactate were significantly higher in death group than those in survival group (P ＜ 0.05).Conclusions There is a good correlation between PCT and APACHE Ⅱ score in patients of AECOPD,suggesting PCT to be a sensitive predictor of prognosis.

s:Primary chronic intestinal pseudo-obstruction (PCIPO)is a rare digestive syndrome characterized by derangement of gut propulsive motility which resembles mechanical obstruction, in the absence of any obstructive process. Treatment of intestinal pseudo-obstruction involves nutritional, pharmacological and surgical therapies, but it is often unsatisfactory and the long-term outcome is generally poor in the majority of cases. This article is aimed at reviewing the current knowledge on etiology, clinical features and management of patients affected by PCIPO.

Pancreatic cancer has a strong ability of invasive and metastasis,and the mechanism is still not completely clear.It has been demonstrated that aberrant expression of microRNAs(miRNA) is closely associated with the occurrence,development and metastasis of pancreatic cancer.The reveal of miRNA mechanism related to the metastasis of pancreatic cancer would provide a new approach to the treatment of pancreatic cancer.

Objective To set up the filter mating method of plasmid conjugation experiment for Corynebacterium.Methods The first plasmid conjugation between different genus was performed by filter method.Three strains of highly erythromycin-resistance Enterococcus faecalis isolates[minimal inhibitory concentration(MIC)of erythromycin>256 mg/L,MIC of levofloxacin≤8 mg/L] which screened by agar dilution method were used as donators and 3 strains of Corynebacteria isolates(MIC of erythromycin≤32mg/L,MIC of levofloxacin=128 mg/L)were used as acceptors.In the second conjugation between the same genus but in different species,three Corynebacterium xerosis strains(MIC of erythromycin>256 mg/L,MIC of ceftazidime=16 mg/L)which were conjugants of the first conjugation were used as donators and 6 strains of Corynebacteria isolates(MIC of erythromycin≤32 mg/L,MIC of ceftazidime 128 mg/L)were used as acceptors.Results In the first conjugation between different genus,the plasimid conjugations were done for 9 times between 3 donators and 3 acceptors,4 of which gained the phenotype and genotype of resistance and the transfer rate was 44%.In the second conjugation experiment,the plasmid conjugations were done for 18 times between 3 donators and 6 acceptors,7 of which gained the resistant phenotype and the transfer rate was 39%.Erythromycin-resistance was transferred from the donators to the conjugants in varying degrees.The resistance was transferred from Enterococcus faecalis to Corynebacteria and then to other Corynebacteria which were of the salne genus but of different species.Conclusion Filter mating conjugation method could be used to study the mechanisms of resistance transmission of Corynebacterium.