The animal experiments,clinical researches and epidemiological investigations indicated that genistein has the effect for prevention and treatment of osteoporosis,cardiovascular disease and cancer.Genistein can inhibit cellular and humoral immunity and can regulate cell apoptosis.The chemical structure,biological effects and the mechanism of genistein attracted much attention.The current research advance on genistein was summarized and the prospect on the theoretical significance,clinical importance was presented in this paper.

Hypothyroidism is one of the most common endocrine disorders in childbearing or pregnant women.Gestational hypothyroidism can increase the incidence rate of the variety of pregnancy complications and adverse pregnancy outcome.It also can make adverse effects on the fetus and neonate,such as death,placental abruption,fetal malformation,intrauterine growth retardation and preterm,low birth weight,neonatal respiratory distress syndrome,it can also cause abnormal thyroid function of neonate,severely can cause nerve,mental,physical and mental developmental abnormalities and even death.Therefore,it is recommended that childbearing women who are at high risk for pregnancy should strengthen to monitor pregnancy thyroid function and treat as soon as possible in order to reduce the adverse effects on offspring.

OBJECTIVE: To evaluate the current situation and trend of narcotic drug use in our hospital where the authors work in order to provide the reference for scientific management and rational use of these drugs. METHODS: The yearly amount of narcotic drugs administered in the whole hospital, the yearly amount in the separate departments, as well as drug expenditures and ratios between January 2002 and November 2005 were added up and analyzed statistically. RESULTS: The amount of bucinnazine use dominated in the first. The amount of morphine for oral use was increasing year by year. The amount of fentangl transdernal patch use was also bigger and increasing rapidly. However, the amount of pethidine and morphine for injection use was decreasing. CONCLUSION:Analgetics for oral and transdermal use will be the main categories of analgetics in the future.

s:Primary chronic intestinal pseudo-obstruction (PCIPO)is a rare digestive syndrome characterized by derangement of gut propulsive motility which resembles mechanical obstruction, in the absence of any obstructive process. Treatment of intestinal pseudo-obstruction involves nutritional, pharmacological and surgical therapies, but it is often unsatisfactory and the long-term outcome is generally poor in the majority of cases. This article is aimed at reviewing the current knowledge on etiology, clinical features and management of patients affected by PCIPO.

Objective To evaluate relationship between p53 codon72 polymorphism and cervical cancer(CC) by using meta-analysis. Methods Studies from 1998 to 2004 were retrieved through Medline, Elsevier, EBMR, Ovid, CNKI and VIP databases without language limitation. The unpublished data of our study was also included. The inclusion criteria of studies were: ① case-control studies associated with p53 codon72 genotype/allele frequencies and CC. ② CC as the outcome of interest. ③ the risk for CC had odds ratio or enough information to calculate it. RevMan4.2 software was applied to process data. Results All studies were analyzed as subgroups according to pathologic histology and race/region. The outcome of CIN of Asia and European population subgroups showed no statistically difference with the control groups. However, in ICC subgroups the frequencies of Arg/Arg genotype were significantly higher than that in the control group(Z=2.43, P=0.01, OR=1.28; Z=2.44, (P=0.01), OR=1.45). Conclusion This study suggests that polymorphism at codon72 of p53 gene is not associated with an increased susceptibility to CIN. However, a slightly increased risk was observed for ICC of Asian and European population subgroups. A possible susceptibility role of p53 codon72 polymorphism at a late carcinogenetic stage of cervical cancer cannot be ruled out.

Objective To detect the phenotype and genotype of 11 vancomycin-resistant enterococci(VRE).Methods Disk diffusion test,microdilution broth method,VITEK-2 and Etest were used to determin the susceptibility of 11 VRE to vancomycin and teicoplanin. MIC to 10 antimicrobial agents were determined with microdiluton broth method. vanA, vanB ,vanC_1, vanC_2 were detected with multiplex PCR and were further confirmed by DNA sequencing. TEM, tetM,ermB,aac(6′)/aph(2′′),ant(6)- I,aph(3′)-Ⅲ were detected with PCR.Results Susceptibilities of 11 VRE to vancomycin and teicoplanin were different in four vitro susceptibility testing methods. VRE showed resistance to erythromycin(9/11),ciprfloxacin(7/11),levofloxacin(7/11),rifampin(8/11) and chloramphenicol(7/11). HLGR and HLSR is 10/11 and 9/11 respectively. 1 VanA,4 VanB,5 VanC_1 and 1 VanC_2 were detected and their phenotypes confirmed to the genotypes. High rate of TEM(8/11), tetM(6/11), ermB(7/11), aac(6′)/aph(2′′)(7/11), ant(6)- I(5/11) and aph(3′)-III(9/11) were detected in 11 VRE.Conclusions VRE screening test and the determination of MIC are reliable in finding VRE. PCR is precise and specific in the testing and confirmation of VRE. The high rates of drug resistance genes showed the complicated mechanisms of drug resistance in VRE.

Objective To investigate the changes of atherosclerosis and vascular tissue after pecutaneous tranluminal angioplasty (PTA) ,and evaluate the role of serum lipid ,lipoprotein (a) [Lp(a)] and tumor necrosis factor‐α(TNF‐α) in this progress .Methods Thirty New Zealand white rabbits with high fate diets were established as observation group ,and 10 rabbits with normal feed were set as control group .Blood biochemistry test was used to detect the levels of serum TC ,TG ,HDL‐C ,LDL‐C ,Lp(a) and TNF‐αbefore the experiment ,after 2 weeks feeding and 1 month ,2 months ,3months after PTA .The correlation among atherosclerosis and Lp(a) ,TNF‐a were measured by multiple logistic regression analysis .Opposite reference value was calculated with receiving operating characteristics (ROC) analysis .Results The levels of TC ,TG and LDL‐C were significant higher than baseline with the feeding time(P<0 .05) .The level of HDL‐C after 2 weeks high cholesterol diet feeding was lower than baseline (P<0 .05) .The content of TNF‐αat 2nd month after PTA was remarkable higher than baseline(P<0 .01) .ROC analysis showed that RR of TNF‐a was 1 .84(P<0 .01) and an area under the curve of 0 .871 for TNF‐α (95% CI:0 .811 -0 .956) compared to 0 .523 for Lp(a) (95% CI:0 .409-0 .720)regarding atherosclerosis .Conclusion The turbulence of lipid could be an important factor of atherosclero‐sis ,and the high level of TNF‐αmay take part in the process of repairing of arteriosclerotic vessel .

Objective:To develop a succinct and effective social support questionnaire for Chinese Farmers.Methods:Based on the studies of Vender Poel Mart et al and combined with the interview data of 53 farmers,a pro-questionnaire was developed;An item analysis was conducted with a sample of 800 farmers of Zhejiang province;CFA was used to test another sample of 807 farmers from Zhejiang and other provinces.Reliability and validity were also tested.Results:Then,9-item questionnaire was formed;EFA indicated that farmers' social support consisted of four factors,namely:instrumental support,company support,emotional support and the utilization degree of social support,which explained 79.23% of total variance;CFA showed the 4-factor model was well fitted;The Cronbaeh's ? of the questionnaire was 0.72 and the split-half coefficient was 0.64;The correlations between 4 factors were 0.10-0.30,and all factor-total correlations were over 0.50.Conclusion:The structure,reliability and validity of Chinese Farmers' Social Support Questionnaire meets the need of psychometrics.

Objective To explore the clinical efficacy of rosuvastatin combined with oxycodone sustained release tablets in patients with advanced non-small cell lung cancer and its effects on serum IL-6 and TNF-αlevels.Methods 62 cases with advanced non-small cell lung cancer from May 2014 to May 2016were divided into observation group and control group according to random number table, each group with 31 cases.Control group were treated with oxycodone sustained-release tablets, and observation group were treated with rosuvastatin and oxycodone sustained-release tablets.The levels of serum IL-6 and TNF-αwere compared pre-and post-treatment betweene two groups.The effective dose, the number of painful pain, the NRS score, the improvement of quality of life of oxycodone hydrochloride sustained-release tablets after treatment and adverse reaction rate were observed. Results After treatment, serum IL-6, TNF-αlevels in two groups were significantly lower than pre-treatment (P <0.05), and which in observation group were significantly lower than control group (P<0.05).Compared with control group, the effective dose of Oxycodone Hydrochloride Prolonged-release Tablets, broke out pain, NRS score in observation group were significantly higher ( P <0.05 ) .The effective rate of cancer pain treatment in observation group was 96.77% (30/31), significantly higher than 74.19% (23/31) of control group (χ2 =12.269, P<0.0001).The improvement of quality of life incontrol group was 32.25%, significantly lower than 80.64% in observation group (P<0.05).There was no significant difference in adverse reactions between two groups.Conclusion Rosuvastatin combined with oxycodone sustained release tablets in treatment with advanced non-small cell lung cancer were effective, and can reduce the patient's serum IL-6, TNF-αlevels effectively.

Objective To study the effect of curcumin ointment on scar formation in filtering blebs after trabeculectomy in rabbits. Methods Twenty-seven rabbits( 27 eyes) were randomly divided into control group,and matrix( placebo) group,and curcumin group,9 in each group. Trabeculectomy for right eyes was performed on all rabbits. Eyes in control group were not treated with any drug,eyes in matrix group were smeared with 0. 1 g common eye ointment ( 3 times a day) ,and eyes in curcumin group were smeared with 0. 1 g curcumin eye ointment ( 3 times a day). Then,intraocular pressure ( IOP) ,filtering blebs,inflammatory cells,collagen,fibroblasts and myofibroblasts were detected by immunohistochemical staining. Results No significant difference was observed in IOP among the 3 groups before and 1,3,28 d after operation. However,a significant difference was found in IOP among the 3 groups in 7,14 and 21 d after operation ( P