Objective To observe the correlation between postmenopausal estrogen levels and diabetic retinopathy (DR) in women.Methods Thirty-nine menopause female patients with type 2 diabetes mellitus and 17 menopause subjects (control group) were enrolled in this study.Control subjects aged from 53 to 82 years,with the mean age of (69.80± 8.32) years.Diabetes mellitus patients aged from 56 to 84 years,with the mean age of (70.50±8.27) years;diabetes duration ranged from 3 to 23 years,with the average course of diabetes (11.40± 7.97) years.DR diagnosis was according to the results of fundus fluorescein angiography,and thus the 39 patients were divided into DR group (19 patients) and non-DR (NDR) group (20 patients).There was no significant difference in age and menopause duration between the three groups (t=0.347,0.485;P＞0.05).There was significant difference in diabetes course (t=2.748,P＜0.05).Compared with NDR group,fasting blood glucose (FBG),glycosylated hemoglobin (HbAlc),total cholesterol (TC),triglyceride (TG),low density lipoprotein cholesterol (LDL-C) were significantly increased (t=6.130,5.322,4.574,2.426,4.033),high density lipoprotein cholesterol (HDL-C) was significantly lower (t=3.917),the difference was statistically significant (P＜0.05).The level of estradiol (E2) was measured by radioimmunoassay.The differences of E2 levels between the three groups were compared.Logistic regression analysis was used to analyze the influencing factors of DR.Results The levels of E2 in control group,DR group and NDR group were (42.38 ±8.64),(21.49 ± 9.81) and (32.72 ± 10.51) pg/ml,respectively.The level of E2 in DR group was significantly lower than that in NDR group and control group (t=3.443,10.110;P＜0.05).Logistic regression analysis showed that the duration of diabetes mellitus [coefficients =0.166,odds ratio (OR)=1.181,P=0.016],FBG (coefficients=1.162,OR=4.014,P=0.001),TC (coefficients=3.212,OR=10.820,P=0.002),TG (coefficients=1.649,OR=5.203,P=0.030) and LDL-C(coefficients=1.605,OR=4.976,P=0.003) were the risk factors for DR;E2 (coefficients=-0.100,OR=0.904,P=0.004) and HDL-C (coefficients=-4.460,OR=0.012,P=0.002) were the protective factors for DR.Conclusion The estrogen level of postmenopausal women have a certain correlation with the development of DR,it may be one of the protective factor of DR.

Biofilms ; Humans ; Sinusitis ; etiology ; microbiology

Cytokines ; Hashimoto Disease ; diagnosis ; prevention & control ; Humans

Objective To investigate the association of platelet-derived growth factor(PDGF)-B gene single nucleotide polymorphism(SNP) and plasma PDGF-BB level with in-stent restenosis(ISR) in elderly patients.Methods 157 patients who had undergone coronary artery stenting for more than half year were divided into ISR group(n=74) and NISR group(non-ISR,n=83) according to the angiographic diagnosis of in-stent restenosis (ISR).Plasma level of PDGF-BB was measured by enzyme-linked immunosorbent assay(ELISA).DNA was isolated from leukocytes.Two SNPs of the PDGF-B gene(rs1800818 and rs1800817) were determined by Taqman Quantitative Real-Time PCR with TaqMan-MGB probe.Results There were no significant differences in genotype frequency of rs1800818 AA,AG,GG between ISR group and NISR group(x2 =4.48,P＞0.05).The frequency of rs1800818A allele was much higher in ISR group than in NISR group(x2 =5.33,P＜0.05).There were no significant differences in genotype frequency of SNP rs1800817 AA and AC(x2 =0.06,P＞ 0.05) and allele frequency of SNP rs1800817 A and C(x2 =0.06,P＞0.05) between ISR group and NISR group,while genotype CC was not found.The plasma level of PDGF-BB was higher in ISR group than in NISR group [(6.53±3.65) ng/L vs.(5.07±2.45) ng/L,t=2.92,P＜0.01].Plasma level of PDGF-BB in patients with rs1800818 AA genotype was significantly higher in ISR group than in NISR group [(9.94 ± 4.60) ng/L vs.(5.90 ± 2.98) ng/L,t =2.69,P＜0.05].Multivariable logistic regression analysis showed that plasma PDGF-BB level was the risk factor for ISR (OR =1.187,95.0％ CI:1.054 1.337,P＜0.01).Conclusions High plasma PDGF-BB level is the risk factor for ISR,but PDGF-B gene SNPs rs1800818 and rs1800817 are not associated with the occurrence of ISR.

Objective To observe the effect of blood-cooling and blood-stasis-removing Decoction on anti-double-strain DNA ( anti-dsDNA) antibody, anti-nucleosome antibody ( AnuA) and serum levels of interleukin ( IL) -6, 17, 21 in spontaneous systemic lupus erythematosus ( SLE) MRL/lpr experimental rats. Methods The experimental rats were divided into blank control group, model group, and high- and low- dose Chinese medicine groups ( 25.2, 12.6 g/kg respectively) , the treatment lasting 8 weeks. At the end of the experiment, the blood taken from the orbital veins was separated for obtaining serum, and then the serum anti-dsDNA antibody, AnuA, IL-6,17,21 levels were tested by enzyme-linked immunosorbent assay ( ELISA). Results The serum autoantibody and IL-6, 17, 21 levels of rats in the model group were increased significantly as compared with the blank control group ( P<0.01). The serum antibody and cytokine levels of Chinese medicine groups were reduced as compared with the model group, the difference between high-dose Chinese medicine group and model group being significant ( P<0.01). Conclusion Blood-cooling and blood-stasis-removing Decoction has certain effect on reducing the serum levels of anti-dsDNA antibody, AnuA, and IL-6,17,21, which may coniribute to one of its therapeutic mechanisms for SLE.

Objective To study the clinical features and significance of the patients with dermatomyositis(DM)associated with interstitial lung disease(ILD).Methods216cases of DM were collected from the Peking Union Medical College Hospital from1970to1997.The criteria for the diagnosis of DM were based on the report of Bohan and Peter in1975.The mean follow-up period of these patients was5.8?4.7years(ranged from2to27years).The clinical manifestations and laboratory findings of32DM patients with ILD were analyzed and compared with those of184DM patients without ILD.Results As compared with DM patients without ILD,the DM patients with ILD were more likely to have fever(28.2%vs.6.5%,P

Objective To discuss the applicated value of systematic nursing intervention in patients with severe pneumonia.Methods 100 patients with severe pneumonia were randomly divided into the study group and the control group,50 cases in each group.The control group was given routine nursing measures.The study group received systematic nursing intervention on the basis of routine nursing measures.The hospitalization time,satisfaction degree, treatment compliance and mentality were observed.Results The SAS scores of the study group before and after nurs-ing were (56.67 ±5.99)points and (38.92 ±5.31 )points,there was statistically significant differences between before and after treatment (t =9.235,P <0.05).The SDS scores of the study group before and after nursing were (62.58 ±6.14)points and (41.15 ±5.26 )points,there was statistically significant difference (t =8.623, P <0.05 ).The hospitalization time ,satisfaction degree and treatment compliance of the study group were (32.9 ± 6.6)days,(8.21 ±1.29)days,6.00%,86.00%,74.00%,which were significantly lower than (45.1 ±10.2)days, (13.56 ±1.77)days,16.00%,52.00%,48.00% of the control group (t =5.254,3.829,χ2 =5.236,8.292, 8.1561,all P <0.05 ).Conclusion Hsystematic nursing intervention can significantly improve the postoperative anxiety and depression,and improve the quality of life of patients with severe pneumonia.

To evaluate the efficacy and safety of limbal relaxing incision ( LRl) for correcting corneal astigmatism during implantable collamer lens ( lCL) surgery.METHODS:A total of 185 eyes of 105 patients with high myopia and corneal keratometric astigmatism were included in the study. lCL surgery with concomitant relaxing incision was performed in 105 eyes of 60 patients in LRls group ( Group A) . Eighty eyes of 45 patients only underwent lCL surgery were in control group ( Group B) . All patients undergone ophthalmic examination that included uncorrected visual acuity ( UCVA ) , best -corrected visual acuity ( BCVA ) , Pentacam analysis system to observe the changes of corneal astigmatism before and 1wk, 1 and 3mo after surgery.RESULTS: Respectively comparing UCVA between two groups in 1 and 3mo postoperatively, the P values were considered statistically significant ( P < 0. 05 ). But, respectively comparing BCVA between two groups in 1wk, 1 and 3mo postoperatively, the P values were considered no statistically significant ( P > 0. 05 ). Preoperative corneal astigmatism was 1. 52 ± 0. 55D in group A and 1. 48 ± 0. 57D in group B, there was no statistically significant difference (P>0. 05). One week postoperatively, the astigmatism was 0. 55 ± 0. 41D in group A and 1. 20 ± 0. 48D in group B. One month postoperatively, the astigmatism was 0. 60 ± 0. 38D in group A and 0. 93 ± 0. 47D. Three months postoperatively, the astigmatism was 0. 51 ± 0. 32D in group A and 0. 96 ± 0. 40D in group B. The difference between the two groups were statistically significant ( P<0. 05 ). The difference value of corned astigmatism before surgery and 1wk, 1 and 3mo after surgery had statistical significance ( P<0. 05). ln LRls group, at preoperative and postoperative time points, the average corneal astigmatism changes were also considered statistically significant difference (P<0. 05). CONCLUSlON: LRls performed during lCL surgery appeared to be an effective and safer procedure to reduce pre-existing corneal astigmatism and improve UCVA as well as the visual quality.

The main results of the article are as follows:GS(10ug/ml,25ug/ml,50ug/ml)could augment NK activity of murine spleen cells in vitro(p

The initial study of the tumour DNA fingerprints using MYO minisatellite DNA probe wascarried out,and then,by means of DNA recombinant techniques,the fragment of MYO min-isatellite DNA probe obtained from plasmid pUC19-MYO was inserted into plasmid pGEM-4Z containing RNA polymerase promotor,thus a sub-clone refferred as pGEM-4Z-MYOwas constucted.That made an offer of the conditions of preparing RNA probe in order to in-cerase the sensitivities of DNA fingerprinting and laid a foundation for raised the efficiency of de-tecting the polymorphism of the minisatellite DNA.