1.Ultrasonographic diagnosis of hepatobiliary cystadenoma
Xiang XU ; Ling CHEN ; Zhen YE
Chinese Journal of Ultrasonography 2009;18(6):496-498
Objective To explore the characteristics of uhrasonography of hepatobiliary cystadenoma. Methods The ultrasonographic characteristics of 5 cases with hepatobiliary cystadenoma were retrospectively analysed, and compared with the operation and pathology. Results The ultrasonography of 5 cases was multilocular cystic mass,which seems to be,but not indeed communicating with the biliary tree,in which irregular septation was demonstrated in two-dimensional structure and also with perforator vessels along in color Doppler flow imagineg. And in some cases small papillary tuft and small loci of calcification was noted on the septation. Conclusions Muhilocular cystic mass,locating along the biliary tree, however not communicating with the the biliary tree, is an important diagnostic basis of biliary cystadenoma. And it should be distinguished with other cystic tumor in the liver.
2. Development and Validation of HPLC-MS/MS Method for Simultaneous Quantitation of Eleven Antipsychotics in Human Plasma
Chinese Pharmaceutical Journal 2018;53(10):808-816
OBJECTIVE: To develop and validate an HPLC-MS/MS method for the measurement of 11 antipsychotics in human plasma samples for TDM(therapeutic drug monitoring) or other objectives. METHODS: Protein precipitation method with acetonitrile was used to extract the drugs in plasma. Agilent Poroshell 120 EC-C18 column(2.1 mm×50 mm, 2.7 μm)was used for chromatographic separation. The mobile phase was acetonitrile with 0.1% formic acid-water with 0.1% formic acid gradiently eluted at a flow rate of 0.3 mLmin-1. The injection volume was 5 μL. The deprotonated ions of analytes were ionized by electrospray(ESI) and detected in positive ionization by multiple reaction monitoring mode(MRM). RESULTS: The total run time of the chromatographic separation and mass detection was 6 min. Excellent linear relationship with correlation coefficient of 0.99 was obtained from 2 to 200 ngmL-1 for risperidone, paliperidone and olanzapine, from 10 to 1 000 ngmL-1 for aripiprazole, quetiapine, amisulpride, clozapine, chlorpromazine and sulpride, from 0.4 to 40 ngmL-1 for haloperidol, and 0.2 to 20 ngmL-1for perphenazine, respectively. The precision and accuracy varied from 2.7% to 10.8% and from 90.7% to 102.7%, respectively. CONCLUSION: This established method is simple, rapid, and economic, fulfilling all criteria for TDM, and could be successfully applied in the routine TDM of antipsychotics in clinic.
3.Expression of WWOX protein and its significance in non-small cell lung cancers.
Yu-long ZHOU ; Yong-jian XU ; Zhen-xiang ZHANG
Chinese Journal of Oncology 2007;29(4):297-297
Adenocarcinoma
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metabolism
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pathology
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Adult
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Aged
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Carcinoma, Non-Small-Cell Lung
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metabolism
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pathology
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Carcinoma, Squamous Cell
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metabolism
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pathology
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Female
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Humans
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Immunohistochemistry
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Lung
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chemistry
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pathology
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Lung Neoplasms
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metabolism
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pathology
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Male
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Middle Aged
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Oxidoreductases
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metabolism
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Sex Factors
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Smoking
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Tumor Suppressor Proteins
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metabolism
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WW Domain-Containing Oxidoreductase
6.Observation on mind-refreshing and orifice-opening needling method plus swallowing disorder therapeutic apparatus for deglutition disorder of stroke patients in the convalescent stage
Zhen-Jie XU ; Lian XIANG ; Xia LIU ; Yi-Fan JIA
Journal of Acupuncture and Tuina Science 2018;16(3):156-160
Objective:To observe the clinical effect of mind-refreshing and orifice-opening needling method plus swallowing disorder therapeutic apparatus for deglutition disorder of stroke patients in convalescence stage.Methods:A total of 136 patients conforming to the inclusion criteria were randomized into three groups by the random number table,including group A of 46 cases,group B of 44 cases and group C of 46 cases.Patients in group A received swallowing disorder therapeutic apparatus treatment,patients in group B received mind-refreshing and orifice-opening needling method treatment,and patients in group C received mind-refreshing and orifice-opening needling method plus swallowing disorder therapeutic apparatus treatment.The treatment was given once a day for 10 d as a course,the whole treatment lasted for 4 courses.Therapeutic evaluation items including water-swallowing test (WST),standardized swallowing assessment (SSA) and modified Barthel index (MBI) were measured before treatment,after treatment and at follow-up visit (2 months after treatment).Results:After treatment,scores of WST and MBI in all three groups increased significantly (all P<0.05),while the SSA score dropped significantly (all P<0.05).After treatment and during follow-up visit period,score of WST in group C was significantly higher than that in group A and group B (both P<0.05),while the difference between group A and group B showed no statistical significance (P>0.05);the SSA score in group C was substantially lower than that in group A and group B (all P<0.05),the difference between group A and group B showed no statistical significance (P>0.05);the MBI scores in group B and group C were substantially higher than that in group A (all P<0.05),the difference between group B and group C showed no statistical significance (P>0.05).After treatment and during follow-up visit period,the differences in overall therapeutic effect between group A and group B showed no statistical significance (P>0.05),while the overall therapeutic effect in group C was substantially better than that in group A and group B (all P<0.05).Conclusion:Both mind-refreshing and orifice-opening needling method and swallowing disorder therapeutic apparatus can alleviate symptoms of deglutition disorder of stroke patients respectively,and the combination of two methods can improve the therapeutic effect.
7.Clinical features and drug sensitivity of lower respiratory tract infection induced by Streptococcus pneumoniae in children
Yun XIANG ; Ruigeng WANG ; Zhen ZHANG ; Xinwen LIU ; Jing XU
Journal of Clinical Pediatrics 2013;(8):715-718
Objective To explore clinical features and drug sensitivity of Streptococcus pneumoniae (SP) isolated from pediatric patients with lower respiratory tract infection, and to provide evidence for clinical use of antibiotics. Methods A total of 6 358 clinical SP isolates from children with lower respiratory tract infection from January 2008 to December 2012 were col-lected and retrospectively analyzed. The antibiotic sensitivity was done by Kirby-Bauer method and E-test, and all results were in strict accordance with the rules of CLSI. Results The isolated SP strains were mainly from different departments of pediatrics. All clinical cases with SP infection mainly included pneumonia and bronchitis. The resistance rates of 6 358 SP strains to penicil-lin, cefotaxime, erythromycin, clindamycin, cotrimoxazole, tetracycline, chloramphenicol, levolfoxacin, vancomycin were 5.0%, 12.9%, 98.7%, 96.0%, 92.2%, 7.3%, 5.6%, 0.2%and 0.0%respectively, and the resistance rate to penicillin and cefotaxime was signiifcantly different in every years (all P<0.05). The resistance rates of the 318 penicillin-resistant SP strains to the above anti-biotics were 100.0%, 42.6%, 100.0%, 100.0%, 99.2%, 23.6%, 6.8%, 0.6%, 0.0%respectively, and the resistance rate to penicillin and cefotaxime was signiifcantly different (P=0.001). Conclusions The antibiotic resistance rates of SP strains isolated from children with lower respiratory tract infection were higher to erythromycin, clindamycin, cotrimoxazole and tetracycline, and an increasing rate in drug resistance to cefotaxime was observed in recent years. Appropriate antibiotics should be selected for the treatment of infection according to drug sensitivity.
8.Effects of Hyperbaric Oxygen on Muscle Strength and Muscle Tone in Patients with Hemiplegia during Atony Stage
Xueyan ZHANG ; Xiang LI ; Xiaomin ZHEN ; Chen XU
Chinese Journal of Rehabilitation Theory and Practice 2010;16(7):653-654
Objective To observe the effects of hyperbaric oxygen on atony after hemiplegia. Methods40 cases with hemiplegia were divided into 2 groups: treatment group (n=17) accepted hyperbaric oxygen and rehabilitation, while control group (n=23) accepted rehabilitation only. The muscle strength, muscle tone and elderly mobility scale (EMS) were measured before and after treatment. ResultsAfter 20 times of treatment, the muscle strength, muscle tone and scores of EMS improved in both groups (P<0.01), and was more significantly in treatment group than in control group (P<0.05). ConclusionHyperbaric oxygen can facilitate the recovery of muscle strength and muscle tone in hemiplegia patients, and improve their motor function.
9.Adenocarcinoma of non-pigmented ciliary epithelium: report of a case.
Mei-Fu GAN ; Zhen-Yang XIANG ; Chun-Kai YU ; Wei-Ming XU
Chinese Journal of Pathology 2008;37(3):213-214
Adenocarcinoma
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pathology
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Aged
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Ciliary Body
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pathology
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Epithelium
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pathology
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Female
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Humans
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Uveal Neoplasms
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pathology
10.Expression of NADPH oxidase subunit p22phox in myocardial infarction rats
Zhihong ZHAO ; Xiaofeng BAO ; Jiang SHAN ; Geng XU ; Guosheng FU ; Meixiang XIANG ; Xiaoye ZHEN
Chinese Journal of Pathophysiology 2000;0(11):-
AIM: To determine the relevance of NADPH oxidase subunit p22hox and the expression of superoxide anion on ventricular remodeling in myocardial infarction (MI) rats. METHODS: MI of Sprague-Dawley rats were established by left anterior descenting coronary artery ligation. 8 weeks after MI, Doppler echocardiography, hemodynamic study and histomorphometry were performed to analyze the ventricular remodeling. The level of thiobarbituric acid reactive substance in plasma and myocardium were measured, and the distribution of superoxide anion was observed with laser scanning confocal microscope. The expression of p22phox mRNA and protein level was detected by RT-PCR and immunohistochemistry. RESULTS: The left ventricular remodeling was significant in MI rats, also the level of thiobarbituric acid reactive substance increased in the plasma and non-infarcted myocardium. The expressions of p22-phox mRNA and protein levels, and superoxide anion increased in infarcted and non-infarcted myocardium in MI rats. CONCLUSION: Our results suggest that the expression of NADPH oxidase and its derived superoxide anion may take part in left ventricular remodeling through oxidative stresss after MI.