1.Chloroplast genome resolution and phylogenetic analysis of Ardisia crispa var. amplifolia and Ardisia crispa var. dielsii
Xian-fa ZENG ; Chang LIU ; Xiao-ying YANG ; Qing YU ; Shi-lun FU ; Teng-yun YAN ; Xiang PU
Acta Pharmaceutica Sinica 2023;57(1):217-228
italic>Ardisia crispa (Thunb.) A. DC. is a traditional Miao medicinal herb with significant therapeutic effects in the treatment of sore throat, tonsillitis, edema of nephritis and bruising and rheumatism, etc.
2.The association study of rs5498 (A/G K469E) and rs1799969 (G/A R241G) in intercellular adhesion molecule 1 gene polymorphism with diabetic peripheral neuropathy in Han population
jie Zhan REN ; yun Xiao TENG ; chang Ke HUANG ; feng Jian YU
Tianjin Medical Journal 2017;45(12):1271-1275
Objective To investigate the association of genetic polymorphisms of intercellular adhesion molecule 1 (ICAM-1) with diabetic peripheral neuropathy (DPN). Methods A total of 607 type 2 diabetes patients from the Affiliated Hospital of Weifang Medical University were enrolled in this study between June 2013 and December 2014. Rs5498 (A/G K469E) and rs1799969 (G/A R241G) in the ICAM-1 gene were genotyped by using TaqMan allelic discrimination in 295 patients with DPN and 312 subjects without DPN. The distribution of these two SNPs and the genetic influence of ICAM-1 gene polymorphisms on the development of DPN were conducted. Results Genotype distributions of both SNPs were coincided with Hardy-Weinberg equilibrium in the two groups. SNP rs1799969 (G/A R241G) in the ICAM-1 gene showed a high GG genotypic frequency at 96.8%(non DPN) and 99.0%(DPN) respectively. SNP rs5498 (A/G K469E) represented AA and AG genotypes. The values were AA 48.7%/AG 39.4%in non DPN group and AA 51.5%/AG 41.7%in DPN group. There were no significant differences in genotypic distributions and allele frequencies of SNPs rs1799969 (G/A R241G) and rs5498 (A/G K469E) between the patients with DPN group and patients without DPN group (P>0.05). The dominant(AA+AG)/GG and additive (GG/AA) models of rs5498 (A/G K469E) were associated with higher risk of DPN (ORadjusted=1.585, 1.575 respectively, P<0.05). To carry A allele was related to the susceptibility of DPN. There was no such association in genetic models of rs1799969 (G/A R241G) and DPN pathogenesis. Conclusion The present study provides evidence that SNP rs5498 E469K (A/G) in the ICAM-1 gene is associated with susceptibility of DPN, and the carrying A allele appears to be a risk of DPN.
3.Effect of Necrostatin-1 on the inflammation in unilateral ureter obstruction mice
Xia XIAO ; Tao ZHANG ; da Xi YANG ; Qing TENG ; Jia HOU ; zhuo Yun REN
Chinese Journal of Clinical and Experimental Pathology 2017;33(8):863-867
Purpose To observe the effect of Necrostatin-1 (Nec-1),a necroptosis inhibitor,on the inflammation in unilateral ureter obstruction mice.Methods Male C57BL/6J mice were randomly divided into sham operation group,unilateral ureter obstruction operation group and UUO + Nec-1 treatment group,and the mice were sacrificed at 7th day after operated.Scr and BUN were measured.The pathological changes in the kidney were observed by HE staining.Immunohistochemistry and Western blot were used to detect the protein expression of necroptosis-related indicators RIP1,RIP3,MLKL and inflammatory cytokines TNF-α,IL-1β and MCP-1.Results Compared with sham operation group,the expression of RIP1,RIP3 and MLKL protein increased in the renal tissue of UUO mice,accompanied with increased expression of TNF-α,IL-1β and MCP-1.Nec-1 treatment significantly decreased above-mentioned protein expression in UUO mice,and also reduced renal interstitial inflammation and renal tubal injury according to HE staining.Scr and BUN levels suggested improved renal function.Conclusion Nec-1 could relieve the inflammatory reaction in renal tissue of the UUO mice by inhibiting necroptosis,which may be a new target for the treatment of secondary inflammation.
4.Advances in research on correlation between microparticle and coronary athero-sclerosis
Chinese Journal of Immunology 2018;34(2):296-300
The microparticle is a kind of small particle formed in the process of cell activation and apoptosis.Currently,the mi-croparticle is taken as the main medium of inflammatory reaction.However,it may also play a role of atherosclerotic plaque formation and subsequent onset,development and formation of acute coronary syndrome.Furthermore,the microparticle participates in the atherosclerotic plaque formation and rupture,coagulation startup and thrombus formation.Through the study of microparticle,the coronary atherosclerotic heart disease pathogenesis can be deeply understood.
5.A meta-analysis on the association of the codon 72 polymorphism in p5 3 gene with the cervical cancer in Chinese population
Fang-Ming YU ; Teng YE ; Yang-Na SU ; Guang-Yun MAO ; Xiao-Ke SHI ; Ben-Long ZHANG
Journal of Preventive Medicine 2016;28(8):770-775
Objective To evaluate the association of cervical cancer and the G/C single nucleotide polymorphism at codon 72 in p53 gene exon -4 in Chinese population.Methods Literatures were searched in CNKI,VIP,CBM, PubMed,EMBase,the Cochrane Library (Issue 1,2015),WanFang Data Database (1980—2015)with computer. Results Thirteen studies involving 1 780 cervical cancer patients and 1 5 17 healthy volunteers were included.According to the situation of the HPV infection in research object,thirteen studies were divided into two subgroups.The results of meta-analyses in the subgroup of non-HPV infection showed that the Chinese women having the genotype G/G presented no increased relative risk compared to that with the genotype C/C(OR=0.980,95%CI:0.558 -1.723).The allele G showed no increased relative risk compared to the allele C (OR=1.070,95%CI:0.789-1.449)The results of meta-analyses in the subgroup of HPV infection showed that the Chinese women having the genotype G/G presented no increased relative risk compared to that with the genotype C/C(OR=0.891,95%CI:0.213 -3.722).The allele G showed no increased relative risk compared to the allele C (OR =0.981,95%CI:0.493 -1.951 ).Conclusion This meta -analysis demonstrates that G/C single nucleotide polymorphism at codon 72 in p53 gene exon-4 were not associated with cervical cancer in Chinese women.
6.Correlation of single nucleotide polymorphisms of X-ray repair cross complementing group 1 gene to hereditary susceptibility of colorectal cancer.
Xiao-dong YANG ; Chun-gen XING ; Kui ZHAO ; Wei GONG ; Yong-you WU ; Yong WU ; Feng-yun ZHONG ; Teng-fei HE
Chinese Journal of Gastrointestinal Surgery 2013;16(12):1195-1198
OBJECTIVETo investigate the correlation of single nucleotide polymorphisms (SNP) of XRCC1 gene to hereditary susceptibility of colorectal cancer.
METHODSXRCC1 genotypes in 124 colorectal cancer patients and 214 matched healthy people as control were analyzed by SnaP Shot SNP-typing technique. Five different inheritance models including codominant, dominant, recessive, overdominant and log-additive were analyzed using logistic regression model. The haplotype distribution was estimated with phase and its correlation with the risk of colorectal cancer was evaluated.
RESULTSThe frequencies of mutant 25487G-A, 25489C-T and 1799782C-T alleles were 0.20, 0.11, 0.32 respectively in the patients, and 0.23, 0.13, 0.34 in the controls. There was no significant correlation of polymophisms of XRCC1 gene to the risk of colorectal cancer in 5 different inheritance models (P>0.05). GCT, GCC, ACC and GTC were the most common haplotypes and the odds ratios were 1, 1.35, 0.90 and 0.84 respectively. There was no significant difference of distribution between 2 groups in haplotypes.
CONCLUSIONPolymorphisms of XRCC1 gene, including rs25487, rs25489, rs1799782, are not associated with to the risk of colorectal cancer.
Colorectal Neoplasms ; genetics ; DNA-Binding Proteins ; genetics ; Female ; Genetic Predisposition to Disease ; Genotype ; Humans ; Logistic Models ; Male ; Middle Aged ; Models, Genetic ; Polymorphism, Single Nucleotide ; X-ray Repair Cross Complementing Protein 1
7.An intervention study to prevent gastric cancer by micro-selenium and large dose of allitridum.
Hao LI ; Hui-qing LI ; Yun WANG ; Hai-xiu XU ; Wan-teng FAN ; Mei-ling WANG ; Pei-Hong SUN ; Xiao-yan XIE
Chinese Medical Journal 2004;117(8):1155-1160
BACKGROUNDPeople have more and more concerned about allitridum as studies have shown that taking more raw garlic associated with a lower risk for cancers of the alimentary system. In the present study, we tried to examine whether a large dose of allitridum and a microdose of selenium prevent gastric cancer.
METHODSA double-blind intervention study was performed on the participants aged (35 - 74) years, who had matched at least one of the following criteria: (1) a medical history of stomach disorder, (2) a family history of tumour, or (3) smoking and/or alcohol consumption. A total of 2,526 and 2,507 persons were randomly enrolled into intervention group and control group respectively from 288 natural villages of seven communities in Qixia County, Shandong Province, China. Each person of the intervention group orally took 200 mg synthetic allitridum every day and 100 microg selenium every other day for one month of each year during November 1989 to December 1991. At the same time, people in control group were given 2 placebo capsules containing corn oid with the identical appearance to that in the intervention group.
RESULTSFor all subjects the large dose of allitridum was accepted and no harmful side effects were found during the study. In the first follow-up five years (1992 - 1997) after stopping the intervention, the morbidity rates of malignant tumours in the intervention group declined by 22%, in contrast to the control group, declined by 47.3%. After adjusting for age, gender, and other potential confounders, relative risks (RRs) for all tumours and gastric cancer of the whole population were 0.67 (95% CL: 0.43 - 1.03) and 0.48 (95% CL: 0.21 - 1.06), respectively, and for male group they were 0.51 (95% CL: 0.30 - 0.85) and 0.36 (95% CL: 0.14 - 0.92), respectively. No signigicantly protective effect was found for the female subgroup.
CONCLUSIONThe present study proves that large doses of allitridum and microdorse of selenium may effectively prevent gastric cancer, especially in men.
Adult ; Aged ; Allyl Compounds ; administration & dosage ; Double-Blind Method ; Female ; Humans ; Male ; Middle Aged ; Multivariate Analysis ; Selenium ; administration & dosage ; Stomach Neoplasms ; prevention & control ; Sulfides ; administration & dosage
8.Efficacy of subgroup mouse bone mesenchymal stem cells on mobilizing autologous cardiac stem cells and repairing ischemic myocardial tissue.
Ji-gang HE ; Zhen-ya SHEN ; Xiao-mei TENG ; Yun-sheng YU ; Hao-yue HUANG ; Wen-xue YE ; Ying-long DING
Chinese Journal of Cardiology 2013;41(3):210-214
OBJECTIVETo search for the bone mesenchymal stem cell (MSC) subgroup which might be more effective on repairing myocardial damage.
METHODSIn this experiment, four MSC subgroups were defined based on the surface differentiation antigen detection of mouse bone mesenchymal stem cells (mBMSCs): SCA-1(+)/CD45(+)/CD31(+), SCA-1(+)/CD45(+)/CD31(-), SCA-1(+)/CD45(-)/CD31(-) and SCA-1(+)/CD45(-)/CD31(+). These subgroup cells and unselected mBMSCs were injected into infarcted mouse via tail vein. Echocardiographic heart function measurement and in vivo DiR-labeled stem cells imaging were performed at 48 h after injection. In situ C-kit (a flag antigen of cardiac stem cells) and cardiac-specific differentiation antigen immunohistochemistry detection was made in the infarcted myocardium.
RESULTSThe capacity of the SCA-1(+)/CD45(+)/CD31(+) cells on improving heart function was significantly higher than other cell groups (all P < 0.05). In vivo imaging showed that the mean fluorescence intensity of the SCA-1(+)/CD45(+)/CD31(+) cells was also higher than other cell groups (all P < 0.05). Number of cardiac stem cells in the infracted myocardium was significantly increased after the injection of all subgroup cells and unsorted mBMSCs cells for 48 h compared untreated infracted myocardium. The capacity of mobilizing cardiac stem cells is as follows: SCA-1(+)/CD45(+)/CD31(+) >SCA-1(+)/CD45(-)/CD31(+) >SCA-1(+)/CD45(-)/CD31(-) >SCA-1(+)/CD45(+)/CD31(-).
CONCLUSIONThe SCA-1(+)/CD45(+)/CD31(+) subgroups of mBMSCs exhibites the highest capacity to improve cardiac function after myocardial infarction and to mobilize autologous cardiac stem cells compared with other mBMSCs subgroups and unsorted mBMSCs cells.
Animals ; Cells, Cultured ; Disease Models, Animal ; Mesenchymal Stem Cell Transplantation ; Mice ; Mice, Inbred C57BL ; Myocardial Infarction ; surgery
9.Hyperspectral characteristics of Carthamus tinctorius in Xinjiang region.
Jin-Xin LIU ; Zheng GUO ; Geng LI ; Jian-Wei YUE ; Heng LU ; Xiao-Teng ZHOU ; Fan-Yun MENG ; Yi-Guang ZHANG
China Journal of Chinese Materia Medica 2013;38(9):1335-1339
Investigation of traditional Chinese medicine resources is the most important issue of the protection and use of traditional Chinese medicine resources. Real-time monitoring of medicinal plant species and coverage of an area are of great significance to the sustainable development of the medicinal plant species diversity and ecological environment. Flower has unique spectral characteristics. Comparing the vegetative stage through the flowering stage it is easier to identify species. The flowering stage is a critical period for identifying species with remote sensing. Carthamus tinctorius as a traditional Chinese medicine resources in XinJiang region, attracted widespread attention in recent years. In this paper, the hyperspectral data of canopy and other vegetation canopy was measured in 2011. The spectral curve was analyzed, the result indicated that C. tinctorius canopy and the canopy after picking showed absorption peak near 770 nm, the first derivative of red edge spectra and invert-Gaussian model were analyzed, the result indicated that there was significant difference between C. tinctorius canopy and other vegetation canopy. The proposed method is designed to provide initial theoretical foundation for growth condition and physiological parameters of C. tinctorius, and make theoretical groundwork for the distribution and elaborate monitoring of C. tinctorius in future.
Carthamus tinctorius
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chemistry
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Medicine, Chinese Traditional
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Plants, Medicinal
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chemistry
10.Role of saliva proteinase 3 in dental caries.
Teng-Yu YANG ; Wen-Jie ZHOU ; Yue DU ; Song-Tao WU ; Wen-Wen YUAN ; Yu YU ; Lin SU ; Yang LUO ; Jie-Hua ZHANG ; Wan-Lu LU ; Xiao-Qian WANG ; Jiao CHEN ; Yun FENG ; Xue-Dong ZHOU ; Ping ZHANG
International Journal of Oral Science 2015;7(3):174-178
Salivary analysis can be used to assess the severity of caries. Of the known salivary proteins, a paucity of information exists concerning the role of proteinase 3 (PR3), a serine protease of the chymotrypsin family, in dental caries. Whole, unstimulated saliva was collected from children with varying degrees of active caries and tested using a Human Protease Array Kit and an enzyme-linked immunosorbent assay. A significantly decreased concentration of salivary PR3 was noted with increasing severity of dental caries (P<0.01); a positive correlation (r=0.87; P<0.01; Pearson's correlation analysis) was also observed between salivary pH and PR3 concentration. In an antibacterial test, a PR3 concentration of 250 ng·mL⁻¹ or higher significantly inhibited Streptococcus mutans UA159 growth after 12 h of incubation (P<0.05). These studies indicate that PR3 is a salivary factor associated with the severity of dental caries, as suggested by the negative relationship between salivary PR3 concentration and the severity of caries as well as the susceptibility of S. mutans to PR3.
Child
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Dental Caries
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enzymology
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Female
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Humans
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Male
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Myeloblastin
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metabolism
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Saliva
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enzymology