Aged ; Aged, 80 and over ; Carcinoma, Hepatocellular ; therapy ; Chemoembolization, Therapeutic ; methods ; Female ; Gelatin ; therapeutic use ; Humans ; Liver Neoplasms ; therapy ; Male ; Middle Aged

Objective To probe the effect of c-fos gene on the pentyleneterazol-induced hippocampal neurons apoptosis.Methods Using immunohistochemistry,TUNEL and flow cytometry(FCM),we detected the Fos expression and the apoptosis of hippocampal neurons;we injected c-fos antisense into ventrile before epilepsy and detected as up.Results Epilepsy can induce the expression of Fos in the hippocampus and peaking at 1 h(P

Objective To investigate the application and feasibility of the single bed work efficiency in the evaluation of hospital beds efficiency,to establish the hospital beds efficiency evaluation model,in order to provide the basis for scientific and effective utilization and evaluation of beds.Methods Proposing the concept of single bed work efficiency,establishing a new evaluation model of bed efficiency,and analyzing the utilization of hospital beds in 2015.Results Single bed work efficiency is supenor to other indexes in evaluating the Utilization efficiency of hospital beds,and the new bed efficiency evaluation model is more objective and accurate.Conclusion The evaluation model of hospital beds utilization efficiency based on the single bed work efficiency is more comparable and operable,which can be widely used in hos pital delicacy management.

AIM: To observe the changes of glutamate and calcium within the hippocampal microenvironment in mitochondrial stress.METHODS: A lateral hippocampus was microperfused with glutamate and calcium chloride solution by a kind of single-pumped push-pull perfusion system in Tree Shrews.At 24 h,the expression of cytochrome C(Cyt C)was observed by immunochemistry.Also,the hippocampus was removed,then mitochondria and cytoplasmic fragment were divided by low temperature centrifugation and the distribution of cytochrome C was assessed through Western blotting.The relative amounts of caspase-3 and caspase-9 mRNA were evaluated by real time fluorescence polymerase chain reaction.In the treated group,cyclosporin A(CsA,40 mg/kg) was intravascularly injected at 6 h after perfusion of glutamate-calcium chloride solutions into the hippocampus and inspected the above-mentioned items at 24 h.RESULTS: In the glutamate-calcium group,compared with the control group,cytochrome C immunoreactivity increased and the content of hippocampal mitochondrial cytochrome C decreased.Also,the cytochrome C was detected in cytosol.Cyclosporin A treatment at 6 h after microperfusion,the cytochrome C expression weakened and no Cyt C in cytosol fraction was observed.By real time PCR,in relation to the control group,the caspase-3 and caspase-9 mRNA was higher in the glutamate-calcium group.Cyclosporin A treatment cut down both caspase-3 and caspase-9 mRNA contents.CONCLUSION: The accumulation of glutamate and calcium may promote Cyt C release,caspase cascade activation and the mitochondrial stress.The neuroprotection of CsA may results from uniquely inhibiting the mitochondrial permeability transition pore,and preventing Cyt C release and caspase activation.

AIM: The present study explored those changes in brain mitochondrial respiration in different times after thrombotic cerebral ischemia induced by photochemical reaction in primate's animal tree shrew and o bserved the effects of platelet-activating factor (PAF) receptor antagonist gin kgolide B (GB) and immunosuppressor cyclosporin A (CsA) on neuronal mitochondria l respiration and mitochondrial permeability transition pore(MPT) in twenty-four hours after occlusion and showed their neuronprotective mechanism and expatiate the relationship between mitochondrial respiration and MPT. METHODS: The focal thrombotic cerebral ischemia was formed by photochemistry-induce d technology in tree shrews. At 4,24,72 h after focal cerebral ischemia, the n euronal mitochondria were centrifuged. Clark oxygen electrode was used to measur e the changes in neuronal mitochondrial respiration.Pretreatment for experiment al animals with GB and CsA at 6h after occlusion, we centrifuged the mitochondri a and measured the changes in neuronal mitochondrial respiration at 24 h after o cclusion. In addition, experiments were performed in a flurometer by measuring CaCl 2 100 ?mol/L induced centrifuged mitochondrial swelling and GB or CsA were added at the same time. RESULTS: All of respiration state Ⅲ, RCR and P/O decreased after cerebral ischemia. There were significant difference s between every ischemic group and sham, especially at 24 h (P

Objective To investigate the accuracy of computer-assisted interpretation(CAI)in the diagnosis of pediatric critical arrhythmia,and to establish Logistic regression model to evaluate the main risk factors of CAI error.Methods 1147 cases of arrhythmia patients were divided into heart disease(HD)and nonheart disease group(NHD).Candidate risk factors were chosen,from which the vailables statistically significant were entered for logistic regression analysis.Results CAI error rate of HD group and NHD group were 24.9%and 11.4%,showing a significant difference(P＜0.05).Age,underlying diseases,the number of CAI diagnosis entries were the risk factors.Conclusion CAI error pattern was different among HD group and the NHD group.Higher awareness for CAI error risk factors and CAI technology improvement are critical for a lower CAI error rate.

To discuss the possibility and methods of pathphysiological bilanguage teaching.The 350 juniors were surveyed by self-designed questionnaire about pathphysiological bilanguage teaching issues.Which appear that most students believe the pathphysiological bilanguage teaching is very important and they hope Chinese and English should be combined.It is necessary to carry out the pathphysiological bilanguage teaching at present,and at the same time explore an appropriate method through practical teaching work.

Child, Preschool ; Diagnostic Errors ; Female ; Humans ; Lung Diseases, Parasitic ; diagnosis ; Pericardial Effusion ; diagnosis ; parasitology ; Pleural Effusion ; diagnosis ; parasitology ; Trematode Infections ; diagnosis

Objective To evaluate the regulatory effect of OX40 co-stimulatory signal on the expression of Foxp3 in inductive regulatory T cells (iTreg) in vitro.Method CD4+ CD25+ naive T cells were isolated from C57BL/6 mouse lymphocyte suspension by MASC CD4+ CD25+ regulatory T cell isolation kit.Inductive Tregs were generated by stimulation of naive T cells in the presence of transforming growth factor beta (TGFβ1),anti-CD3,anti-CD28 and IL-2.The regulatory effect on iTregs was shown by use of OX40 stimulation monoclonal antibody (OX86) or control antibody.Using flow cytometric analysis (FACS),we examined the antibody-based identification of Tregs surface markers CD4 and CD25,along with the intracellular activation marker FoxP3.Results The ratio of CD4+ CD25+ nTregs isolated from mouse lymphatic node was (5.0 ± 0.4)％ vs.(71.8 ± 13.4)％ of TGFβ1-driven iTregs.The ratio of CD4+ CD25+ Tregs was (80.0 ± 1.6) ％ in OX40 stimulation McAb group vs.(86.0 ± 1.4)％ in control antibody group.Furthermore,the expression of Foxp3 was (59.2 ± 0.7) ％ in OX40 stimulation McAb group vs.(70.0 ± 0.8) ％ in control antibody group (P＜0.05).Conclusion TGFβ1-dependent protocol may induce the conversion of naive CD4+ T cells into CD25+ Foxp3+ iTregs.OX40 stimulation can down-regulate the expression of Foxp3 in CD4+ CD25 + iTreg significantly.Thus OX40 molecular may become an attractive target in Tregs-induced transplant tolerance.Further study should be performed to increase the suppressive activity of iTregs through blockade of OX40 signal.

Objective To provide references for building the medical rehabilitation system in China by learning the progress and compliance of rehabilitation departments construction at tertiary hospitals. Methods Comparative and quantitative methods were used for dynamic analysis qualitative interview to learn the index compliance of the hospitals in question in 201 1-2012.Results Compared with 201 1, average days of stay of the rehabilitation departments declined in general,yet with insufficient therapists;introduction of early rehabilitation intervention was but 57.1%,and the functional assessment rate of rehabilitation service was less than satisfactory.Conclusion Lack of manpower,varying levels of medical rehabilitation services,and neglect for functional assessment were found to be the main problems in construction of medical rehabilitation departments for the time being.