Objective:To provide the methodology reference for the gene expression data clustering.Methods:This paper proposed a fuzzy clustering ensemble algorithm based on wavelet de-noising.The new method is applied to the yeast cell's gene expression data,and the clustering results were compared with fuzzy ensemble clustering methods.Results:Apply micro-precision to evaluate the clustering results.The research indicated that the proposed method was superior to fuzzy ensemble clustering algotuthm.Conclusion:The proposed method has better clustering for the gene expression data clustering.

Graduate medical education, postgraduate medical education and continuing medi-cal education between China and the United States were compared in order to explore a better physi-cian training system of China. The physician training system of the United States has a series of ad-vantages such as strict access system, uniform training standard and closely linked among each stage, etc. We should make clear medical education standards and strengthen the cohesion between each stage, standardize the management mode and perfect the management system and promote regular as-sessment system.

BACKGROUND: PI3K is a family of enzymes involved in insulin signal transductlon pathway, the abnormal expression of which would affect synthesis, secretion, and migration of GLUT4, therefore, results In increased blood sugar, eventually, leads to diabetes.OBJECTIVE: To discuss the effects of insulin combined with selenium on PI3K and GLUT4 expression in cellular signal transduction of skeletal musde in diabetic rats.METHODS: Sprague Dawley (SD) rats were randomly divided into the control, diabetic, insulin-treated diabetic, selenium-treated diabetic and combination administration groups. All rats were prepared for diabetic models by injecting 50 mg/kg Streptozocin exception of the control group. Rats in the control and diabetic groups ware free to food and water; in the insulin treatment group,rats were subcutaneous injected 1 U/(kg·d) insulin. Rats in the selenium treatment group ware treated with a dose of 180 pg/kg per day of sodium selenite dissolved in redistilled water by gavage; and in the combination administration were given both treatments for 4 successive weeks. The levels PI3K end GLUT4 in skeletal muscle were estimated using Western blotting and immunohistochemistry techniques.RESULTS AND CONCLUSION: Immunohietochemistral results were accordance to Western blotting results, which showed the combination of insulin and selenium can remarkable Increase the levels of PI3K in skeletal muscle and GLUT4 in skeletal muscle membrane fraction, therefore, enhance the insulin signal transduction pathway.

Objective To investigate the relation between omentin and coronary artery disease.Methods Eighty-eight eases were divided into 2 groups, coronary artery disease group (56 cases, includingacute coronary syndrome 28 cases and stable angina 28 cases), and non-coronary artery disease group (32cases). The plasma omentin levels were assayed by ELISA, and height, weight, blood pressure, totalcholesterol high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C),lriglyeeride (TG), high-sensitivity C-reactive protein were detected. Results The plasma omentin level incoronary artery disease group [(33.4 + 6.4) μg/L] was lower than that in non-coronary artery disease group [(51.8 + 7.7) μg/L](P= 0.034). Logistic analysis showed that the decline of omentin was an independent risk factor of coronary artery disease (P=0.012). Conclusions The decline of omentin is an independent risk factor of coronary artery disease. The plasma omentin may contribute, in part, to the pathogenesis of coronary artery disease.

Objective To investigate the relation between plasma omentin and glycometabolism.Methods Eighty-five cases were divided into 3 groups by glucose tolerance test:diabetes mellitus group (42cases), impaired glucose regulation group (17 cases) and normal glucose regulation group (26 cases). The level of plasma omentin was assayed by ELISA. Results The level of plasma omentin was (53.7 ± 7.7),(40.7 ±9.9) and (30.6 ±5.3) μg/L in diabetes mellitus group, impaired glucose regulation group and normal glucose regulation group. There was significant difference among three groups (P= 0.049) and there was significant difference between diabetes mellitus group and normal glucose regulation group (P= 0.014).The level of plasma omentin in normal glycometabolism was (53.7 ± 7.7) μ g/L , and the level of plasma omentin in abnormal glycometabolism was (33.5 ± 6.0) μ g/L. There was significant difference between them(P = 0.023 ). Logistic analysis showed that the decline of plasma omentin was the independent risk factor ofglycometabolic disturbance (P= 0.027). Conclusion Omentin may play important roles in glycometabolic disturbance.

Objective To study the effect of cellulase on the extraction rate of medlar total saccharide, considering three factors of temperature, time and quantity of cellulose. The best extracting condition was determined according to the require of sphere symmetry design. Methods Add cellulase liquid into the medlar slurry and determine the content of medlar total saccharide by DNS method. Result The extraction rate of medlar total saccharide was increased 1.66% by cellulase at temperature of 60 ℃, time of 300 s and cellulase volume of 5 mL. Conclusion Cellulase can promote the extraction rate of medlar total saccharide.

【Objective】To optimize the functional monomers which is used for the synthesis of Corilagin molecular imprinting polymer.【Methods】The chromatographic characteristics of Corilagin molecular imprinting polymers synthesized respectively by functional monomers of ?-methacrylic acid(MAA)and acrylamied(AA)were investigated with HPLC,and the imprinting efficiency of the two kinds of polymers on Colilagin template were compared.【Results】The polymer with AA as the functional monomer had a higher imprinting efficiency than the polymer with MAA as the functional monomer.【Conclusion】AA is the optimal functional monomer for the synthesis of Corilagin molecular imprinting polymer.

Bone remodeling is an ordered process of coupling of bone resorption and bone formation. Cytokines secreted by different bone cells and membrane molecules expressed by bone cells are involved in regulation of coupling of bone resorption and bone formation. To reveal the mechanism of bone coupling is of great significance in the study and diagnosis of osteoporosis and other metabolic bone diseases. In the present review, we summarize the progression of mechanisms by which cytokines and the related membrane molecules are regulating the coupling of bone resorption and bone formation.

This report emphasizes on US diagnostic characteristics of partly BSTT,including OSteogenic sarcotma in 57 cases,chondrosarcoma in 25 cases, fibrosarcoma in 18 cases, synovial sarcoma in 14 cases, myelogenous neoplasm in 21 cases.metastatic bone ttmlor in 67 cases,giant cell turnor of bone in 52 cases, chordoma in 25 cases,hemangioma In 32 cases,desmoplastic fibroma in 10 cases. Pigrnenled villonodular synovius in 10 cases,and aneurysmal bone cyst in 8 cases, altogether 563 cases. The study firstly presents the application and specific menifestation of US diagnosis in BSTI. In many aspects,US is superor to X-ray, CT and MRI in diagnosing BSTT. It provides a great deal of reliable basis for clinical diagnosis and. Treatment.

Objective To evaluate the effect of remifentanil on heart rate variability,to discuss the optimal does of remifentanil for tracheal intubation.Methods Thirty six ASA Ⅰ～Ⅱ patients were randomly assigned to one of three groups(n=12/group)according to the remifentanil target plasma concentration(2、4、6ng/mL).No premedication was given.The target-controlled infusion(TCI)of renifentanil was initiated 5 minutes after the TCI of propofol given at the target plasma concentration of 3.5?g/mL.Mean arterial pressure(MAP),heart rate(HR),HF,LF,HF/LF were measured from baseline values to 5 minutes after the operation began every minutes.MAP,HR,HF,LF and HF/LF were obtained on the time point as follows:before the induction(T0),before the start of remifentanil TCI(T1),before laryngoscopy(T2),and maximum values after intubation(T3),before operation(T4),maximum values after operation.All the data obtained were analyzed used ANOVA with SPSS 11.5 statistical software.P