Objective To investigate the clinical manifestations,laboratory features,methods of diagnosis and treatment and prognosis outcome of intrahepatic cholestasis caused by citrin deficiency (NICCD).Methods Four patients in two months ages diagnosed as NICCD were investigated from February 2014 to December 2014 in the Children's Hospital of Wuhan.The diagnosis and treatment and prognosis were analyzes.Results All patients were in hospital due to the skin with yellow dye admission.The direct bilirubin elevated anomalies associated with different levels of blood lipid,blood ammonia and lactate metabolism were characterized by liver function tests.The hot spot of NICCD SLC25A13 gene mutations in the regional common mutations IVS13 + 1G/A,1638ins23,IVS11 + 1G/A,851del4,S225X,1800ins1,R605X,IVS6 + 5G ＞ A were detected by genscaning and genotyping.Genotyping of SLC25A13 gene in 4 cases were 1638ins23 and 851del4 two heterozygous mutations,851del4 homozygous mutation,851del4 heterozygous mutation,and IVS6+ 5G＞A heterozygous mutation,respectively.So these patients were confirmed as NICCD.The patients were cured by antiinfective and gallbladder back yellow symptomatic treatments.The liver function,blood ammonia,lactic acid and blood lipid were exanimated at the time of admission,1 weeks and 2 weeks after admission,respectively.The results indicated that the treatment effect were good.The liver function were restored to normal after two weeks of outpatient.Conclusion The diagnosis of NICCD needs a comprehensive analysis of clinical,biochemical,metabolic genomics,imaging and pathology.SLC25A13 gene analysis is the reliable basis for the diagnosis of the disease.It is the key for NICCD to diagnose early.It is important to distinguish bile deposition caused by biliary atresia and other related diseases from NICCD.The diet early replacement is good for the prognosis.

Objective:To explore the effect of Nimodipine on synaptophysin in diabetic rat.Methods:38 Wistar rats were divided into the normal control group, diabetic model group, the Nimodipine treatment group randomly. Streptozotocin-diabetic rat model was established. The diabetic rats were treated with Nimodipine for 12 weeks. Then, the learning and memory abilities of rats were tested with the Morris water maze.The mRNA and protein level of synaptophysin in hippocampus were detected by RT-PCR and immunohistochemical method. Results:Compared with diabetic model group, the Nimodipine treatment group showed a significant decrease in the mean time of escape latencies(P

Objective:To evaluate the values of the endoscopic argon plasma coagulation (APC) in the treatment of gastrointestinal polyps. Methods:The gastrointestinal polyps confirmed by endoscopy and pathology were treated with argon plasma coagulation generated by ERBE APC 300 made in German ERBE Company. Results:A total of 78 polyps (59 flat and thin sessile polyps and 19 pedunculated polyps) in 23 patients were all cured with APC. 59 flat, thin and sessile polyps were only treated with APC, whereas 19 pedunculated polyps were treated with snared polypectomy and APC ablation of residual tissue. Time of coagulation for every polyp was determined by its size and location. Polyps became flat and thin after(1～7)times of coagulation. As many as 10 polyps could be treated at one time, but for patients with more than 10 polyps, several times of treatment were needed. Conclusion:Treatment of gastrointestinal polyps by APC is an effective and safety method with low side effects. It can be the first choice of methods for the treatment of flat and thin sessile gastrointestinal polyps or polypoid projection.

Objective To investigate the incidence of the common respiratory viruses in children with respiratory tract infection and to compare the results among different ages and to analyze the relationship between wheezing of children and various viruses.Methods Nasopharyngeal secretions of 618 children with respiratory infection hospitalized were assayed for 7 respiratory viruses, including respiratory syncytial virus ,adenovirus, influenza A virus, influenza B virus, parainfluenza virus Ⅰ 、 Ⅱ、 Ⅲ.Results (1) A total of 166 cases, affected with viral etiology,were detected in 618 samples,and the total positive rate was 26.86％.The most common virus was RSV (17.96％), followed by PIV Ⅲ (5.99％), IFA (1.62％), ADV (0.81％), PIV Ⅰ (0.49％).Moreover, 1 case infected with RSV and PIVⅠ was included, with the positive rate 0.16％.(2)The positive rates were different in various ages, with 14d ～ ＜ 1-year 35.04％, 1 ～ ＜ 3-year 18.25％, 3 ～ ＜ 6-year 17.11％ and ≥ 6-year 9.26％.(3) There were differences of positive rate of viruses among different respiratory infectious diseases, and capillary bronchitis occupied the highest percentage(47.37％).(4)Among all the detected children, infants were more likely to whee in auscultation of lung, and RSV was the primary etiology.Conclusion RSV,which was the primary viral etiology in children with wheezing, was the most common virus leading to respiratory infection of children, followed by PIV Ⅲ.In the children with respiratory tract infection, the viral infection tends to occur in younger children.

Objective　To study the visual electrophyological changes in　patients with pregnancy-induced hypertension syndrome(PIH).Methods　The visual evoked potentials (VEP) and electroretinogram (ERG) of 28 patients with PIH in 56 eyes were recorded.Results　The patients with PIH had pathologic visual electrophysiologic abnormalities. The VEP N75 peak latency significantly increased(P<0.05), and the ERG b wave latency in patients with PIH showed remarkable difference(P<0.05).Conclusion　Visual electrophysiological examination may be valuable in early diagnosis of retinal disfunction in patients with PIH.

Portal vein tumor thrombus (PVTT) influence the prognosis of hepatocellular carcinoma.The development of PVTT is a multi-factor,multi-part process.According to anatomic features of the portal vein in the liver and tumor thrombus of HCC developing modes,a uniform tumor thrombus types system (typesⅠ -Ⅳ) is recommended.Multi-modal therapy based on surgery,interventional therapy and radiotherapy can improve the curative effect enormously.

Circulating tumor cells (CTCs) play pivotal roles for monitoring the tumor metastasis and prognosis.The nanotechnology provides a favourable platform for CTCs detection,and enables CTCs to be more promising for practical application.Meanwhile,the nanoscale device by virtue of nanotechnology has broad application prospects in eliminating CTCs and offers a new direction in the field of anti-cancer.

Children who recovered from hematological malignancies have different degrees of secondary immunodeficiency.They are prone to acquire various infectious diseases, thus affecting their health and even endangering their life and may become the source of infection that affects the health of the surrounding people and residents of co-mmunities.Preventive vaccination is the easiest and most effective measure to prevent infectious diseases.However, in addition to the limited qualification of vaccination, due to the lack of the understanding to the safety, effectiveness and contraindications in the vaccination for this type of patients, there still exists blankness in the preventive vaccination work for this group in China.This paper summarizes some consensus on the vaccination for discharged patients of hematological malignancies in light of providing reference for the implementation of this clinical work in China.

Urticaria, which is called as“yin zhen”in traditional Chinese medicine, is a kind of allergic skin disease with comings and goings of wind clumps patches. Its episodes have close relationship with external infection of six evil factors. This article collected, classified, and studied literature of TCM doctors in different dynasties about urticaria and external infection of six evil factors, and expounded pathogenesis of urticaria in the aspects of wind pathogen, dampness pathogen, fire evil and other pathogenic factors. In the treatment of urticaria, internal treatment is divided into dispelling wind method, eliminating dampness method, clearing heat method and other methods. External treatment is concluded form the aspects of washout method, medicated ironing method, and moxibustion method. This article classified and dig some valuable information, with a purpose to provide reference and evidence for prevention and treatment of urticaria.

Objective To study genetic polymorphism of surface adhesion protein 33 (AP33) gene on the seven isolates of Trichomonas vaginalis. Methods PCR technique was performed to amplify AP33 gene from the seven isolates, DNA sequences were obtained from the AP33 gene of the isolates and phylogenetic tree was built. Minimal lethal concentrations(MLC) of metronidazole on the isolates were measured in vitro. Results Percentage of the similarity between 7 isolates and U87098 in GenBank was 98.2%-100%,which indicated a high homology and belonged to isotype isolates. There were four branches between Beijing 1 isolate and Tangshan isolate, Jiujiang 1 isolate and Jiujiang 2 isolate, Beijing 2 isolate and Jiujiang 3 isolate, Chengde isolate and U87098 isolate in phylogenetic tree, which showed a close genetic relationship respectively. No relativity was detected between geographical origin and genetic relationship. Conclusion There is a close genetic relationship among the seven T. vaginalis isolates. MLC showed a difference between isolates which have close relationship.