2. Analgesic effects of aspirin combined with kv7 channel openers
Chinese Pharmacological Bulletin 2020;36(2):267-270
Aim To provide scientific evidence for clinical drug combination by exploring the analgesic effect of aspirin combined with Kv7 channel openers, retigabine and flupirtine. Methods The mice were randomly divided into control group, aspirin group, flupirtine group, retigabine group, aspirin + flupirtine group and aspirin + retigabine group. To assess the antinociceptive effects of each group, the acetic acid-induced abdominal constriction test and the hot-plate test were used. Results In the acetic acid-induced abdominal constriction test, compared to aspirin group, the amount of abdominal constriction in aspirin + flupirtine group was significantly reduced (P <0.01). The latent period of abdominal constriction in aspirin + retigabine group was significantly prolonged ( P < 0. 01) and the number of abdominal constriction was significantly reduced (P < 0. 01). In the hot-plate test, compared to aspirin group, the increment percentage of latent period in aspirin + flupirtine group and aspirin + retigabine group showed an increased trend. Among them, the increment percentage of latent period 30 and 60 minutes after injections in aspirin + retigabine group had a significant difference from that in aspirin group (P <0. 05). Conclusion Kv7 channel openers, retigabine and flupirtine, can enhance the analgesic effect of aspirin.
3.Comparison of Antiviral Therapy Recommended by 2015 Guidelines for Management of Chronic Hepatitis B
Wei ZHANG ; Yu WANG ; Jidong JIA
Chinese Journal of Gastroenterology 2016;21(4):193-196
This review article compared the antiviral therapies recommended by major international and national guidelines for management of chronic hepatitis B(CHB)issued by American Association for the Study of Liver Diseases, Asian-Pacific Association for the Study of the Liver,Chinese Society of Hepatology & Chinese Society of Infectious Diseases,China Medical Association,and World Health Organization in 2015. The essentials and highlights of guidelines were compared,focusing on goals of therapy,indications of therapy,choices of drugs,endpoints and duration of therapy, management of treatment failure,treatment of CHB in pregnancy.
4.Role of p38 mitogen activated protein kinase signaling pathway in lung ischemia-reperfusion protection offered by HOE642
Jia DENG ; Yu SHI ; Xiyang ZHANG ; Yabing ZHANG ; Bin LIU
Journal of Central South University(Medical Sciences) 2017;42(7):749-754
Objective:To explore the protective effect of sodium channels antagonists HOE642 on lung ischemia reperfusion and the role of the p38 mitogen activated protein kinase (p38MAPK) signaling pathway in this process.Methods:A total of 36 mice were randomly divided into a sham operation group (SHAM group),a lung ischemia reperfusion group (I/R group) and a lung ischemia reperfusion+HOE642 group (HOE group).The water content was detected by electronic scales,and the lung tissue pathological changes were observed under optical microscope.The inflammatory cytokines including IL-6 and TNF-α were examined by ELISA.The intracellular calcium fluorescence intensity was examined and observed under fluorescence microscope,and the protein expression of p38MAPK was detected by Western blot.Results:Lung water content in the HOE group was lower than that in the I/R group,but higher than that in the SHAM group (both P<0.05).Lung interstitial edema,hemorrhage,lung tissue inflammatory cells infiltration were significantly alleviated in the HOE group than those in the I/R group,while the injury in the HOE group was aggravated than those in the SHAM group (both P<0.05).T he IL-6 and TNF-α in lung tissues in the HOE group were lower than those in the I/R group,but higher than those in the SHAM group (both P<0.05).Intracellular calcium fluorescence intensity in the HOE group was lower than that in the I/R group,but higher than that in the SHAM group (both P<0.05).The protein expression of p38MAPK in lung tissues in the HOE group was lower than that in the I/R group,but higher than that in the SHAM group (both P<0.05).Conclusion:HOE642 may exert protective effect on pulmonary I/R injury through regulation of the p38MAPK signaling pathway,resulting in reduction of intracellular calcium ion concentration and calcium overload,and decrease of inflammatory response.
5.The value of lipid accumulation product index on screening carotid atherosclerosis in females in Dalian area
Nan ZHANG ; Xiaofang PAN ; Xiaodong JIA ; Bo ZHANG ; Hao YU
Chinese Journal of Postgraduates of Medicine 2021;44(1):34-39
Objective:To explore the relationship between the lipid accumulation product index (LAP) and the risk of carotid atherosclerosis in different gender groups.Methods:The data of 2 740 employees of Dalian municipal government and institutions, including 1 583 males and 1 157 females, who had underwent physical examination in Dalian Municipal Central Hospital Affiliated of Dalian Medical University from January to June 2018 were analyzed retrospectively. The general data, laboratory indexes and carotid ultrasound data of the population were collected, and the body mass index (BMI) and LAP value were calculated. According to carotid intima-media thickness (IMT), the subjects were divided into carotid atherosclerosis group (IMT ≥ 1.0 mm) and carotid normal group (IMT<1.0 mm). The clinical indicators between the two groups were compared. Independent risk factors for carotid atherosclerosis were analyzed by binary Logistic regression, and the cutoff point for predicting carotid atherosclerosis by LAP was calculated by receiver operating characteristic (ROC) curve.Results:The age, waist circumference (WC), hip circumference (HC), LAP, BMI, total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), fasting plasma glucose (FPG), systemic blood pressure (SBP), diastolic blood pressure (DBP) and the prevalence of hypertension and diabetes in the male and female population carotid atherosclerosis groups were significantly higher than those in the normal group ( P<0.05). The results of binary Logistic regression analysis showed that after adjusting age, BMI, HC, WC, smoking, drinking, hypertension, diabetes, TC and LDL-C, the risk of carotid arteriosclerosis still increased 1.316 times with each quartile interval of LAP in women ( OR= 1.316, 95% CI 1.063 to 1.629, P = 0.012). In men, after adjusting for these factors, the independent effect of LAP on the risk of carotid atherosclerosis disappeared. In women, the area under curve (AUC) of LAP in predicting carotid atherosclerosis was 0.713, significantly higher than that of BMI (AUC 0.614) and WC (AUC 0.654), and the differences were statistically significant ( P<0.05). But there was no significant difference among the three indexes in the male population in predicting carotid atherosclerosis. According to ROC curve, the best cut-off value of LAP for predicting carotid atherosclerosis was 32.13 cm·mmol/L in men and 33.70 cm·mmol/L in women. Conclusions:LAP is an independent risk factor for carotid atherosclerosis in female population, and its predictive ability for carotid atherosclerosis is higher than that of body mass index and waist circumference. LAP ≥ 33.70 cm·mmol/L can be used as the best cut-off value for screening carotid atherosclerosis in female population.
6.Determination of Related Substances in Methylphenidate Hydrochloride for Injection by HPLC
Xueling ZHANG ; Chaozhong WANG ; Shoushi JIA ; Xiaoqiu YU ; Liancheng ZHANG
China Pharmacy 2017;28(12):1711-1713
OBJECTIVE:To establish a method for the determination of related substances in Methylphenidate hydrochloride for injection. METHODS:HPLC method was adopted. The determination was performed on Waters symmetry C18 column with mo-bile phase consisted of methanol-0.01 mol/L potassium dihydrogen phosphate solution(60:40,V/V)at the flow rate of 1.0 mL/min. The detection wavelength was set at 210 nm,the column temperature was 35 ℃ and sample size was 10 μL. RESULTS:The linear range of impurity A and B were 0.02-3.0 μg/mL(r=0.9998). The limits of quantitation were 0.2,0.6 ng,and the limits of detec-tion were 0.06,0.2 ng,respectively. RSDs of precision,stability and reproducibility were all lower than 2.0%;recoveries were 98.2%-100.0%(RSD=0.56%,n=9),98.0%-100.3%(RSD=0.70%,n=9),respectively. CONCLUSIONS:The method is sim-ple,accurate and suitable for the determination of related substance in Methylphenidate hydrochloride for injection.
7."Analysis on Academic Characteristics of ""Jing-Zhu Materia Medica"""
Jia YU ; Yi ZHANG ; Jia NIE ; Dan ZHANG ; Silang JIANGYONG ; Zangjia GENG
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(1):112-115
Tibetan ancient literature is an important foundation for the development of Tibetan medicine and clinical research. The essential features and advantages of inheritance and development of Tibetan medicine play an ines-timable role. Jing-Zhu Materia Medica is by far the most important Tibetan medicine monograph. This book is rich in content, national characteristics, featured prominent plateau, which is practical in the clinical application. This ar-ticle analyzed the literature value and academic characteristics of Jing-Zhu Materia Medica from the drug classifi-cation, medicinal processing method, and comparison between Tibetan medicine and Chinese medicine. It promoted the analysis of ancient Tibetan medicine literatures to provide references for the scientific research, medical practice and application development of Tibetan medicine.
8.Role of Nrf2/ARE singling pathway in reduction of myocardial ischemia-reperfusion injury by zinc sulfate preconditioning in rats
Jia NIE ; Haiying WANG ; Yongguo ZHANG ; Weibo HOU ; Tian YU
Chinese Journal of Anesthesiology 2017;37(3):370-374
Objective To evaluate the role of nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) singling pathway in reduction of myocardial ischemia-reperfusion (I/R) injury by zinc sulfate preconditioning in rats.Methods SPF healthy male Sprague-Dawley rats,weighing 250-280 g,aged 16-20 weeks,were used in this study.After the animals were anesthetized,their hearts were immediately removed and retrogradely perfused with an oxygenated K-H solution at 37 ℃ in a Langendorff apparatus.Forty isolated rat hearts were randomly divided into 4 groups (n=10 each):control group (group C),I/R group,zinc sulfate preconditioning group (group Zn) and zinc sulfate preconditioning plus Nrf2/ARE singling pathway blocker luteolin group (group Zn+Lut).After 20 min of equilibration,the hearts were continuously perfused for 100 min in group C,the hearts were perfused with 4 ℃ St.Thomas′ cardioplegic solution before ischemia and then subjected to 40 min global ischemia followed by 60 min reperfusion to establish the model of I/R in group I/R,200 μmol/L zinc sulfate 1.5 ml/kg was injected intraperitoneally,and 24 h later the model of I/R was established in group Zn,and in group Zn+Lut,the hearts were perfused for 3 min with K-H solution containing luteolin 50 μmol/L starting from the time point immediately before ischemia,and the other treatments were similar to those previously described in group Zn.Heart rate (HR),left ventricular end diabetic pressure (LVEDP),left ventricular developed pressure (LVDP) and the maximum rate of increase of left ventricular pressure (+dp/dtmax) were recorded at the end of equilibration and reperfusion.Coronary effluent was collected at the end of reperfusion to measure the levels of lactate dehydrogenase (LDH) and malondialdehyde (MDA).The expression of heme oxygenase-1 (HO-1),quinone oxidoreductase (NQO1),superoxide dismutase 1 (SOD1) and Nrf2 in myocardial tissues was detected by Western blot.Results Compared with group C,HR,+dp/dtmax and LVDP were significantly decreased,LVEDP was increased,and the levels of LDH and MDA in coronary effluent were increased at the end of reperfusion in I/R and Zn+Lut groups,and the expression of NQO1,HO-1,Nrf2 and SOD1 was up-regulated in I/R,Zn and Zn+Lut groups (P<0.05).Compared with group I/R,the HR,+dp/dtmax and LVDP were significantly increased,LVEDP was decreased,and the levels of LDH and MDA in coronary effluent were decreased at the end of reperfusion,and the expression of NQO1,HO-1,Nrf2 and SOD1 was up-regulated in group Zn (P<0.05).Compared with group Zn,HR,+dp/dtmax and LVDP were significantly decreased,LVEDP was increased,and the levels of LDH and MDA in coronary effluent were increased at the end of reperfusion,and the expression of NQO1,HO-1 and SOD1 was down-regulated(P<0.05),and no significant change was found in Nrf2 expression in group Zn+Lut (P>0.05).Conclusion The mechanism by which zinc sulfate preconditioning reduces myocardial I/R injury is related to activation of Nrf2/ARE singling pathway in rats.
9.Studies on The Supercondensed pBR322 DNA Isolated From Escherichia coli topA-Mutant
Zhenfeng ZHANG ; Jia YU ; Kou CAO ; Xitai HUANG
Progress in Biochemistry and Biophysics 2006;0(03):-
The supercondensed DNA, a special kind of topological structure of plasmid DNA, was firstly found in E. coli SD108(topA+ gyrB225). Now, this structure is also found in E. coli DM800(topA- gyrB225). The result indicates that the formation of supercondensed DNA is related with decrease of the activity of gyrase in vivo. Topoisomerase Ⅳ was proved to relax the supercondensed DNA completely in vitro, which suggested that the supercondensed DNA and the supercoiled DNA could transform to each other in cells. The supercondensed DNA samples were analyzed by atomic force microscopy and compared to supercoiled DNA. The results showed that the length of supercondensed DNA decreased about 30% and the width and height of double-strand increased about 60%, which indicates that the structure of double-strand of supercondensed DNA is much more similar to A-DNA than B-DNA. The results also showed that chloroquine intercalation did not change the supercoiling level of supercondensed DNA, but made it knot and compact.
10.Down-regulation of FTO in human gastric cancer and its effect on cell line MGC-803 function
Jingnan PI ; Jie ZHANG ; Xuepeng WANG ; Jiachao XIONG ; Jia YU
Basic & Clinical Medicine 2017;37(7):907-911
Objective To investigate the expression of FTO in gastric cancer tissues and the functional significance of FTO in MGC-803 cell line.Methods The FTO mRNA was detected by RT-qPCR in 54 cases of gastric cancer samples and their paired adjacent normal control tissues.The effect of FTO overexpression in MGC-803 on cell proliferation,cell migration and invasion were detected by CCK-8,wound heal and ranswell assays,respectively.Results The expression of FTO mRNA was significantly lower than that of adjacent tissues(P<O.05).Furthermore,overexpression of FTO in MGC-803 cells inhibited cell proliferation,cell migration and invasion.Conclusions FTO is low expressed in gastric cancer tissues and inhibits gastric cancer cell line MGC-803 proliferation,migration and invasion.FTO is closely associated with the development of gastric cancer.