This paper mainly discussed how Chinese archaic ideologist realize emotionality. The contents include five aspects: emotionality is related to human nature and morality; Chinese pay more attention to cultivate himself according to morality, control his emotion and need, keep harmonious relationship with others, emotionality is in association with or- gans. The author thought that the meaning of Chinese emotionality is not only the emotional reaction of oneself, but in- volves comprehensive features of individual emotional response, dealing with matters and behaviors, and it is closely relat- ed to the cultivation of morality and human nature and relative to human nature and morality. Chinese opinion is different from western psychologists’thoughts.

Objective To explore related factors of amputation in patients with diabetes foot ulcer(DFU) and provide the evidence of reducing amputation rate.Methods A total of 307 inpatients with DFU in this hospital from October 2013 to December 2015 were collected and divided into amputation group(n=28) and non-amputation group(n=279).The clinical data and laboratory data were treated with single variable analysis and multivariate Logistic regression analysis.Results Single variable analysis showed that hospitalization time,hyper-sensitive C-reactive(hs-CRP) and fibrinogen(FIB) level were significantly increased in amputation group(P＜0.05) when compared with non-amputation group,and the hemoglobin (Hb) level,high-density lipoprotein cholesterol(HDL) and serum albumin (ALB) level in amputation group were markedly lower than in non-amputation group(P＜0.05).Amputation rate was significantly different among different Wagner grades (P=0.000).Multivariate Logistic regression analysis showed that hospitalization time,Wagner grade were independent risk factors of amputation in patients with DFU,OR values were 1.024(95％CI:1.009-1.039) and 2.779 (95％CI:1.753-4.404),respectively.While HDL was a protective factor of amputation in patient with DFU (OR=0.161;95％CI:0.036-0.729).Conclusion The higher the rank of Wanger in DFU patients are,the longer hospital stay and the higher amputation risk become.HDL is the protective factor of DFU amputation.

Objective To evaluate the levels of oxidative stress in patients undergoing long-term and repetitive exposure to hyperbaric oxygen (HBO) treatment. Methods 16 healthy volunteers and 58 patients with sub-acute sudden hearing loss (SHL) exposed to HBO were included in the study. Oxidative stress indices (malondialdehyde, MDA, advanced oxidation protein products, AOPP; superoxide dismutase, SOD) were measured in peripheral blood samples collected at the 5th,10th, 20th and 30th HBO treatments sessions (PO2 0.18 MPa, 1 session per day and 5 sessions per week) and under normal ambient pressure respectively. Results After 5th,10th, 20th and 30th sessions of HBOT, no relevant differences in these three indices were detected compared to pre-HBO exposure, between healthy volunteers (P > 0.05). Conclusions The long-term repetitive HBO treatment for 0.18 MPa of PO2 and 30 sessions could not affect in particular the response of the oxidative stress in healthy persons and patients with sub-acute SHL. The influence on three indices of patients with abnormal situation of oxidative stress undergoing lower pressure of HBO (0.18 MPa) is under investigation.

Objective To evaluate the biophysical properties of insulin-producing cells labeled and unlabeled with superparamagnctic iron oxide nanoparticals and poly-L-lysine(SPIO-PLL)in vitro,and then monitor cellular imaging with 1.5 T MR.Methods BMSCs were isolated from tibia and femur of 6～8 weeks normal Spragne-Dawley rats,purified on the basis of their ability to adhere to the matrix,and expanded through their self-renewal.Two-step strategy was adopted with BMSCs induced into insulin-producing cells, After that,the cells were incubated with SPIO-PLL.Prussian blue stain was employed for identifying intracellular irons.Radioimmunology assay was used to measure the insulin secretion by the labeled and unlabeled cells,and later on underwent MR imaging with T_1WI、T_2WI、T_2*WI sequences,Results lntracytoplasmic nanoparticales were stained with Prussian blue possessing insulin-producing cells labeled with SPIO-PLL.The amount of insulin secreted by the labeled and unlabeled ceils had no statistical significant difference.The signal intensity of labeled cells decreased significantly on T_2*WI,as well as the stronger proportional variations for signal intensity.Conclusion Insulin-producing cells can be labelled effectively with SPIO-PLL and be imaged by 1.5 T MRI.(J Intervent Radiol,2007,16:104-108)

OBJECTIVETo study the effect of phenformin hydrochloride that may be illegally added in traditional Chinese medicine preparations on the pharmacokinetics of puerarin in rats.

METHODRats were randomly divided into the single pueraria group and the phenformin hydrochloride combined with pueraria group. After oral administration in the two groups, their bloods were sampled at different time points to determine the drug concentration of puerarin in rat blood and calculate pharmacokinetic parameters.

RESULTAfter oral administration with pueraria extracts and phenformin hydrochloride combined with pueraria extracts, the two groups showed main pharmacokinetic parameters as follows: Cmax were (2.39 +/- 1.01), (1.03 +/- 0.35) mg x L(-1), respectively; Tmax were (0.50 +/- 0.09), (1.5 +/- 0.5) h, respectively; Ke were (0.153 +/- 0.028), (0.172 +/- 0.042) h(-1), respectively; t(1/2) were (4.65 +/- 0.86), (4.20 +/- 0.81) h, respectively; AUC(0-t), were (5.73 +/- 2.60), (5.45 +/- 1.81) mg x h x L(-1), respectively; AUC(0-infinity) were (6.72 +/- 2.89), (6.26 +/- 1.88) mg x h x L(-1), respectively. Compared with the single puerarin group, the Cmax was significantly decreased (P < 0.05) and the Tmax was markedly longer (P < 0.01) than the hydrochloride combined with pueraria group.

CONCLUSIONPhenformin hydrochloride can slow down the absorption process of puerarin and change the pharmacokinetic process of puerarin to some extent.

Administration, Oral ; Animals ; Drug Interactions ; Hypoglycemic Agents ; administration & dosage ; pharmacology ; Isoflavones ; administration & dosage ; pharmacokinetics ; Male ; Phenformin ; administration & dosage ; pharmacology ; Rats ; Rats, Wistar ; Vasodilator Agents ; administration & dosage ; pharmacokinetics

Objective To invesgate the effect of P-glycoprotein(PGP)inhibitor,verapamil,on electrobiological activity and seizure behavior in phenytoin-carbamazepine(PHT-CBZ)resistant rats.Methods The model of medically intractable epilepsy was established by kindling of amygdale. Verapamil was applied to PHT-CBZ resistant rats,followed by the observation on after discharge threshold (ADT),after discharge duration(ADD)and seizure activity.Results Compared with the control group, the ADT was higher in PHT-CBZ resistant rats peritoneally injected with verapamil((238.0?32.2)?A vs (177.0?23.3)?A,P

OBJECTIVE: To establish a reliable, exact and practical method to prepare DNA samples for sensitivity-test purposes. METHODS: The micromanipulation method was employed to prepare exact quantity DNA samples used to study the sensitivity of Profiler Plus Kit-ABI PRISM 310 system. RESULTS: We succeed in establishing a micromanipulation method to prepare groups of DNA samples, which contain 1-11 cells in turn, and also succeed in using them to study the sensitivity of Profiler Plus Kit-ABI310 system. CONCLUSION The method we have established is proved to be a reliable, exact and practical way to prepare DNA samples for sensitivity-test purposes.
DNA/isolation & purification* ; DNA Fingerprinting/methods* ; Microscopy ; Polymerase Chain Reaction ; Sensitivity and Specificity ; Specimen Handling/methods* ; Tandem Repeat Sequences

OBJECTIVE: MicroRNAs (miRNAs) play a vital role in pathogenesis and progression of many cancers, including cervical cancer. However, importance of serum level of miR-101 in cervical cancer has rarely been studied. In the present study, clinical significance and prognostic value of serum miR-101 for cervical cancer was investigated. METHODS: Association between miR-101 level in cervical cancer tissues and prognosis of patients was analyzed by using data retrieved from The Cancer Genome Atlas (TCGA) database, which was followed with our clinical study in which miR-101 serum level comparison between cervical cancer patients and healthy controls was conducted by real-time quantitative polymerase chain reaction (PCR). RESULTS: TCGA database demonstrated that miR-101 was down-regulated in cervical cancer tissues compared with normal cervical tissues, and univariate Cox regression analysis indicated that decreased miR-101 expression was a highly significant negative risk factor. Similar trend was found in the serum miR-101. Serum level of miR-101 was associated with International Federation of Gynecology and Obstetrics (FIGO) stage (p=0.003), lymph node metastasis (p=0.001), and serum squamous cell carcinoma antigen (SCC-Ag) level >4 (p=0.007). The overall survival time of cervical cancer patients with a higher level of serum miR-101 was significantly longer than that of patients with a lower level of serum miR-101. Moreover, multivariate Cox regression analysis indicated that the down-regulated serum level of miR-101 was an independent predictor for the unfavorable prognosis of cervical cancer. CONCLUSION: Serum level of miR-101 is closely associated with metastasis and prognosis of cervical cancer; and, hence could be a potential biomarker and prognostic predictor for cervical cancer.
Carcinoma, Squamous Cell ; Clinical Study ; Disease Progression ; Genome ; Gynecology ; Humans ; Lymph Nodes ; MicroRNAs ; Neoplasm Metastasis ; Obstetrics ; Polymerase Chain Reaction ; Prognosis* ; Risk Factors ; Uterine Cervical Neoplasms*

Objective To explore dynamic response of serum lipids and relationship with body mass index(BMI)after fat meal in obese children and adolescents. Methods The subjects were 31 obese children and adolescents (BMI ≥ 25 kg/m2) and 30 controls (BMI

Objective To compare the clinical manifestations of gastroenteritis caused by norovirus and rotavirus in infants and young children in Beijing. Methods Stool specimens were collected from infants and young children with acute diarrhea who visited the Affiliated Children's Hospital to Capital Institute of Pediatrics from January 2002 to December 2006. Registration form was designed for clinical data collection for each patient from whom specimen was collected. Poly-acrylamide gel electrophoresis (PAGE) and enzyme immunoassay (EIA) were used to detect rotavirus and Human norovirus, respectively. Results Among 779 stool specimens tested for rotavirus, 263 were positive (33.8 %), and norovirus positive specimens were 79 out of 318 (24.8%) specimens tested. Most of the clinical manifestations of gastroenteritis caused by these two viruses were quite similar with no significant difference, except for fever. The seasonal distribution of these two viruses were different with the peak of rotavirus infection was in cold weather between October and January, as indicated by the peak of the positive rates of the virus detection. The infection of norovirus seemed no obvious peak in the year.Conclusion Rotavirus is the most important pathogen for acute diarrhea among infants and young children while. Norovirus is also an important pathogen for acute gastroenteritis in infants and young children. No significant difference was found out for clinical manifestations for the gastroenteritis caused by these two viruses.