OBJECTIVE: To observation the effects of Glycosaminoglycan from Scallop Skirt (SS-GAG) on the production of vasoactive substance in the endothelial cells injured by hydrogen peroxide(H2O2). METHODS: In H2O2-induced en-dothelial cell oxidative damage model group which was established with human umbilical vein endothelial cell (HUVEC, ECV304) cultured in vitro, SS-GAG groups (at different concentrations) and the normal group, levels of 6-keto-PGF1a, Thromboxane-B2 and Endothelin (ET) in the culture fluid were detected by radioimmunity assay and the content of nitric oxide was determined by nitrate reductase method. RESULTS: As compared with model group, SS-GAG (at different concentrations) significantly enhanced the levels of 6-keto-PGF1a and NO but significantly decreased levels of TXB2 and ET (P

AIM:To establish a fingerprint method of analyzing Xuefu Zhuyu Capsule(Semen Persicae,Radix Angelicae sinensis,Fruclus Aurantii,Rhizoma Chuanxiong,etc.) by HPLC. METHODS: A HPLC in combination with PDA detector was applied with a HiQ C_(18)(4.6 mm?250 mm,5 ?m) column by gradient elution using methanol-1.0% acetic acid solution as the mobile phase.The flow rate was 0.5 mL/min and the detection wavelength was at 260 nm. RESULTS: Thitrty-six common peaks were obtained in the fingerprint of Xuefu Zhuyu Capsule with good separation,six of them were identified as adenosine,paeoniflorin,naringin,hesperidin,neohesperidin and ligustilide by comparing chromatogram with control compounds. CONCLUSION: The HPLC method with good precision and reproducibility can be used for the quality control of Xuefu Zhuyu Capsule.

Child ; Humans ; Urolithiasis ; diagnosis ; therapy

To investigate the correlation between traditional Chinese medicine (TCM) syndrome and short-term prognosis of ischemic stroke.

Objective To investigate epigenetic silencing of the proapoptotic gene MAPK10 by methylation in B-cell lymphoma. Methods We examined MAPK10 expression and methylation in seven cell lines derived from B-cell lymphoma by semi-quantitative RT-PCR and methylation-specific PCR (MSP), respectively. Methylation status was further examined in 24 diffuse large B-cell lymphoma (DLBCL), 15 follicular lymphoma (FL) and 12 reactive hyperplasia lymph nodes (LRH) both by MSP and bisulfite genomic sequencing (BGS). Results MAPK10 is silenced or downregulated in all seven B-cell lymphoma cell lines mostly due to promoter methylation. MAPK10 methylation was frequently detected in DLBCL (17 of 24, 71%) and FL (15 of 15, 100 %), but not in 12 LRH tissues. Conclusion MAPK10 is frequently inactivated by tumor-specific methylation, and thus, is a potential biomarker.

Psychometrical methodology was widely applied in the development of TCM syndrome scales,without considering the consistency between the methods and clinical TCM syndrome differentiation practice and the difference between the syndrome diagnosis and syndrome evaluationt.In this article,we described the theory foundation of the TCM syndrome diagnosis scales,distinguished the difference between the syndrome diagnosis scales and syndrome evaluation scales from several aspects,and expounded the key steps and technical point of TCM syndrome diagnosis scales development superfi cially.Ischemic Stroke TCM Syndrome Scales based on these represented a satisfying performance in clinical validation.

Objective: To establish the quality control standard for Xiaozhi Solution(Radix et Rhizoma Rhei, Radix Scutellariae, Radix Paeoniae Rubra, Rhizoma Smilacis Glabrae, etc.) Methods: Radix et Rhizoma Rhei Radix Scutellariae、 and Radix Paeoniae Rubra in solution were identified by TLC. The contents of emodin chrysophanol were determined by HPLC. The average recovery of added sample were 99.10% ( RSD was 1.88% ) for emodin and 98.87% ( RSD was 1.21%) for chrysophanol. Results: The method was simple, accurate and specific. Conclusion: The method can be used for the quality control of Xiaozhi Solution.

Objective: The experiment was undertaken to explore the blood-brain barrier permeability and the gene expression of matrix metalloproteinase-9 (MMP-9) in ischemic cerebra and the impact of Buyanghuanwu Decoction on them. Methods: The (middle cerebral artery occlusion, MCAO) rat model was used. At 1h, 3h, 24h, 48h, 5d after reperfusion. They were measured. Results: In treatment group, the BBB permeability and the MMP-9 gene expression decreased. Conclusion: Buyanghuanwu Decoction can protect the BBB by inhibiting the MMP-9 gene expression.

Objective To study how to prevent the persistent ectopic pregnancy(PEP)effectively after the conservative celioscope surgery.Methods After conservative celioscope surgery,MTX(Group A)and the traditional chinese medicine(Group B)were taken to the people who had ectopic pregnancy to prevent PEP.Results 2 examples in group A had PEP,as well as non-PEP occurred in group B.Conclusion The function of the traditional chinese medicine after conservative celioscope surgery to prevent the persistent ectopic pregnancy was much better.

Objective To discuss incidence and related risk factors of vascular cognitive impairment (VCI) after acute subcortical infarction (SI).Methods One hundred and eighty-two patients with acute SI were divided into two groups:VCI group (81 cases) and no-VCI group ( 101 cases) according to VCI diagnostic standard.Sex proportion,age,culture degree between two groups were compared,and correlation was analyzed between the quantity of risk factors and the incidence of VCI.Results The incidence of VCI after acute SI was 44.5％(81/182).According to age stratification,the incidence of VCI in the patients of 56-65 years was 35.4％ (23/65),in the patients of 66-75 years was 45.3％ (48/106),and in the patients of 76-85 years was 90.9％ (10/11 ).The age in VCI group was significantly higher than that in no-VCI group [(69.93 ±6.91) years vs. (67.62 ±5.56) years,P =0.014],and culture degree was significantly lower than that in no-VCI group (P =0.028).The sex proportion between two groups had no significant difference (P =0.876).The more accompanying risk factors,the higher incidence of VCI.Conclusions Along with the increase of age,the incidence of VCI after acute SI is higher.The more accompanying risk factors,the higher incidence of VCI.