Thyroglobulin antibody (TGA) production in vitro by cultured peripheral blood mononuclear cells (PBMC) from patients with autoimmune thyroid disease (AITD) was measured by ELISA technique. PBMC from patients with AITD could synthesize and secret detectable amount of TGA. The average positive rate of TGA production in vitro was 31.25%. PBMC from the patients with Hashimoto thyroiditis (HT) could produce TGA more easily than that from the patients with Graves'disease (GD). The average positive rate was 44.19% in HT and 16.22% in GD. Pokeweed mitogen could increase TGA production, but soluable thyroglobulin could not stimulate TGA synthesis and secretion. Antithyroid drug methi-mazole had some inhibition effect on TGA production in vitro. A significant positive correlation between the serum TGA and the amount of TGA produced in vitro by PBMC from HT patients (r = 0.4, P

Lithium(Li) salt is. a kind of effective drug commonly used in the treatment and prevention of mental disorders, but its active mechanism is still not well understood. Getting on for twenty years, many research have been reported on relationship between Li salt and second messenger system, especially the effect of Li salt on adenylate cyclase. Identical result is not obtained and there are few reports on brain calmodulin (CaM) activity change by Li salt. The aim of present expcri- ment is to determine the content of CaM in raet brain by phosphodiesterase (PED) method after chronic application of Li salt and discuss preliminary the molecular mechanism of Li salt action. The result showed that marked change has happened in the content of CaM in rat brain after chronic application of Li salt. It is ins-plied that Li salt brings its action into play probably by changing the content of CaM in rat brain

The annual incidence of prostate cancer (PCa) continually increases. New PCa immune therapy has become the fourth line antitumor treatment following surgery, radiotherapy and chemotherapy. As the most promising research direction in cancer immunotherapy,immune checkpoint inhibitors, such as programmed cell death-1 and programmed cell death-ligand 1 (PD-1/PD-L1) inhibitors, block PD-1 and its ligand PD-L1 and then terminate the negative control signal to the T cell, thereby facilitating T cell recovery and reversal of tumor immune-escaping mechanism. These processes restore the capability of T cells for immune response and inhibit and kill tumor cells. This review summarizes the progress on the current application of PD-1/PD-L1 inhibitors in PCa clinical trials.

Objective: To discuss the methods and experience of 64-slice spiral CT which used in infant cases with congenital heart disease, to select the appropriate scan parameters for reducing artifact and the radiation dose. Methods: Thirty-five cases with congenital heart disease were arranged CT examination by using Philips Brilliance 64-slice spiral CT scanner with Blous Tracking techniques. Results: All the images of the heart chambers, pulmonary artery, pulmonary vein, the ascending aorta and the descending aorta contrast agent filling showed good and had clear display. The density of superior vena cava is moderate. The various vascular CT value is approximate, no hardening artifacts. The atrioventricular of Cardiac and vascular of contrast agent filled fully and had clear boundary, no significant artifacts and no effect on the impact reconstruction. Conclusion: Appropriate adjustment of scanning parameters can reduce hardening artifact and the impact of artifacts. Following the principle of as low as reasonably achievable (ALARA) can obtain diagnostic images and reduce radiation damage to the infant.

Medicine, Chinese Traditional ; Teaching ; methods ; Terminology as Topic ; Translations

Objective To understand the death level and main death causes among residents in the Wansheng Economic De‐velopment Zone to provide the basis for comprehensive prevention and control .Methods According to the ICD‐10 international classification of diseases ,the death causes were classified and coded for statistically analyzing the crude mortality rate ,standardized mortality rate and the death causes constituents in this surveyed population .Results The crude mortality rate of residents in Wan‐sheng Economic Development Zone during 2014 was 650 .57/100 000 and the standardized mortality rate was 413 .35/100 000 .The sequence of death causes was circulatory system disease ,respiratory system disease ,tumor ,injury and poisoning and digestive sys‐tem diseases .The death number for these 5 kinds of death causes accounted for 93 .21% of the total death number .The top five of potential life loss years were injury and poisoning ,tumor ,circulatory system disease ,respiratory system disease and digestive system diseases .The rates of potential years of life loss were 13 .76‰ ,10 .19‰ ,8 .35‰ ,4 .84‰ and 2 .69‰ respectively .Conclusion Chronic noncommunicable diseases ,injury and poisoning become the main causes of death in the Wansheng Economic Development Zone and disease burden .

Objective To observe the effects of Kinesio taping on upper limb spasticity after stroke. Methods From March to Novem-ber, 2015, 39 stroke patients were divided into Kinesio taping group (Kinesio taping and rehabilitation, n=13), white taping group (white tap-ing and rehabilitation, n=13) and blank group (rehabilitation, n=13) randomly. They were evaluated with modified Ashworth Scale (MAS), Fugl-Meyer Assessment (FMA) of upper limbs and the modified Barthel Index (MBI) before treatment, four and eight weeks after treat-ment. Results There was no significant difference among the three groups in the scores of FMA and MBI (P>0.05) four weeks after treat-ment, but the score of MAS improved more in the Kinesio taping group than in the other two groups (P<0.05). The scores of MAS, FMA and MBI all improved more in the Kinesio taping group than in the other two groups (P<0.05) eight weeks after treatment. Conclusion The application of Kinesio taping can relieve upper limb spasticity, and improve motor function and activities of daily living for patients with stroke.

Selective serotonin reuptake inhibitors (SSRIs),a new generation of anti-depressant agent,are mainly used to treat emotional disorders in clinical practice now.Recent studies have found that SSRIs can increase neurogenesis after cerebral ischemia,promote neurovascular reconstruction,and are conducive to the recovery of neurological function.Furthermore,SSRIs can improve the tolerance of ischemic tissue,exert anti-inflammation and anti-oxidation properties,reduce the blood-brain-barrier injury,and inhibit excitatory amino acid toxicity in order to exert neuroprotective effect.This article reviews the related research progress in SSRIs and ischemic stroke.

ObjectiveTo observe the clinical efficacy of acupoint thread embedding plus psychotherapy for insomnia due to disharmony betweenheart and kidney during perimenopause.MethodForty-three patients with insomnia due to disharmony between heart and kidney during perimenopause were intervened by acupoint thread embedding plus psychotherapy including cognitive and behavioral therapies,and then the therapeutic results were statistically analyzed.ResultOf the 43 subjects, 19 were clinically cured, accounting for 44.2%; 13 subjects were markedly improved, accounting for 30.2%; 9 were improved, accountingfor 20.9%; 2 subjects failed, accounting for 4.7%. The total effective rate was 95.3%. The serum E2level was significantly changed after intervention (P<0.01).ConclusionAcupoint thread embedding plus psychotherapy can produce a significant efficacy in treating insomnia due to disharmony between heart and kidney during perimenopause, and this method can up-regulate the serum E2level, enhance the production of estrogen, and improve ovary function.

Bone Marrow Mesenchymal Stem Cells (BMSCs) are ideal seed cells for cell therapy,which have been through many long-term studies of in vitro and animal disease model treatment and have been widely used in preventing and treating a variety of clinical diseases caused by aging and other damage,including bone injury and regeneration,neural degenerative and injury,myocardial injury,liver cirrhosis,diabetes,lung disease and so on.BMSCs application in the treatment of orthopaedic disease is the most common and effective.By retrospective literature review,we summarize the BMSCs transplantation application in Orthopaedic disease treatment.As an ideal cell source,BMSCs was first applied in bone tissue engineering,mainly used in the treatment of long bone defect and bone nonunion;Recently,the research trends of clinical BMSCs application turned to treat degenerative diseases and genetic disorders because of its great potential,including osteoarthrits,femoral head necrosis,disc degeneration,spinal cord injury,knee varus,osteogenesis hypoplasia,hypophosphatasia and so on.The safety and efficacy of BMSCs treatment are the key issues in clinical cell therapy.There is no side effects and complications after BMSCs treatment,prove its clinical application is safe.But due to type and degree of disease and individual differences,therapeutic methods including injection method,effective concentrations,treatment window selection,cycle and therapeatic effects have a bigger difference.There is still no uniform of recognized operation standards for the treatment of the same type of disease.So BMSCs transplantation requires effective control during whole medical process and its application in orthopedic diseases is still lack of large-scale multicenter clinical study.Therefore,this review focuses on induction,summary and analysis in the research status of BMSCs in bone disease treatment,and provide new ideas and methods for BMSCs transplantation in orthopaedic disease prevention and treatment.