Objective To study the therapeutic procedure,indication and effect of breast conserving surgery(BCS) on early-stage breast cancer patients.Methods 48 cases carried BCS plus chemotherapy and radiotherapy had been follow-up for 3～49 months.Results All cases were alive and none had local recurrence.41 cases(85.7%) were satisfactory with the breast appearance,The remaining 7(15.3%) got poor appearance.Conclusion BCS therapy is suit to early breast cancer patients both for cosmetic and function to elevate quality of life of patients.And it should be practiced widespreadly.But it be cared about that mastering indication strictly,operation methods and postoperation radiotherapy are the key factors for achieving good result.

Objective To investigate the expression and clinical signification of tumor M2 pyruvate kinase in pa-tients with CRC, and to try to find a reliable and atraumatic method to predict the progress and pronosis in the CRC. Methods ELISA method was used to determine the serum levels of tM2-PK in 68 patients with CRC,40 pa-tients with colorectal polyps and 40 cases of normal subjects; immunohistochemistry ( IHC ) methods were respec-tively used to detect the expression of tM2-PK in tissues. Results ① The level of serum tM2-PK in patients with CRC was significantly higher than the group of patients with polyps and the normal subjects ( P<0.05 ) . The serum tM2-PK levels was closely correlated to tumor size and Duke stage.②The positive rate of tissue tM2-PK was signif-icantly higher in CRC than in colorectal polyps and normal subjects ( P<0.05 ) . Tissue tM2-PK levels was closely correlated to tumor size,Duke stage and differentiation grade. ③Serum tM2-PK levels was correlated to tissue tM2-PK levels (r=0.357,P=0.003). Conclusion Combined detection of expression of tM2-PK can provide the basis theory for the diagnosis and prognosis evaluation of CRC.

Objective To investigate the effect of tunor necrosis factor alpha (TNF-α) antagonist treatment and traditional disease-modifying antirheumatic drugs treatment on the quality of life of male patients with ankylosing spondylitis (AS).Methods In 42 patients with AS open-label study,patients were grouped after consent,and divided into the TNF-α antagonist treatment group and the non-TNF-α antagonist treatment group.All patients were treated for 4 months.The clinical and laboratory indexes and quality of life changes were analyzed before and after the treatment.T test,Pearson's correlation analysis were used for statistical analysis with software version 13.0.Results There were no significant differences in age,disease course,disease activity and the quality of life between the two groups before treatment.Disease activity and the quality of life of the two groups after treatment were improved compared with that of before treatment.After treatment,the Bath ankylosing spondylitis disease activity index (BASDAI) score [(1.9±1.6) scores,(3.0±1.3) scores,t=-2.429,P=0.020],erythrocyte sedimentation rate (ESR) [(9±6) mm/1 h,(18±17) mm/1 h,t=-2.286,P=0.031] and C reactive protein(CRP) levels [(18±21) mg/L,(62±85) mg/L,t=-2.258,P=0.035] of the TNF-α antagonists treatment group decreased significantly than those non-TNF-α antagonist treatment group,while the hemoglobin (Hb) levels [(143±15) g/L,(138±18) g/L,t=2.545,P=0.015] were significantly increased in TNF-α antagonist treatment group.The improvement extent of quality of life was more evident in TNF-α antagonists treatment group,such as,the average score [(72± 15) scores,(55±19) scores,t =3.254,P=0.002].The average degree of improvement in quality of life and BASDAI score,Bath ankylosing spondylitis functional index (BASFI) score were negatively related to the improvement in the TNF-α treatment group (r=-0.497,P=0.018; r=-0.558,P=0.007).Conclusion TNF-α antagonist treatment can not only improve the AS disease activity,but can also improve the quality of sexual life of male patients,which may direct affect on male reproductive system.

It has been proved that severe acute respiratory syndrome (SARS) is caused by a novel coronavirus. Detecting disease in its early stage, understanding its pathways of transmission and implementing specific prevention measures for the disease are very important in the course of disease control. China has established the definitions of suspected and confirmed cases of SARS. But the laboratory tests and definitions for the diagnosis are limited.Now the primary measures for SARS include isolation, therapeutics application with ribavirin and corticosteroid, mechanical ventilation, etc. Convalescent plasma is being explored.There still are many problems to be solved in the course of conquering SARS.

The numbers of eosinophils and mast cells expressing 5-lipoxygenase of airway in aspirin-induced asthma (AIA) increased. LTC 4 synthase, the terminal enzyme for cys-LT production, is overexpressed markedly in bronchial biopsy specimens of most patients with AIA. These characterized the unique airway inflammation of AIA which may illuminat the pathogenesis of AIA to some extent.

Benign lymphoepithelial lesion(BLEL) is known as Mikulicz disease.The clinical manifestation of BLEL mainly includes diffuse painless enlargement of the eye or the monocular lachrymal gland.The etiology and pathogenesis of the disease proposed mainly concerns the sex hormone hypothesis,transforming growth factor-β involvement hypothesis,basal cell hyperplasia invasion hypothesis,and IgG4-related hypothesis,etc..The typical pathological changes are infiltration of lymphocytes in the gland,atrophy of the gland tissue,formation of myoepithelial islands within the duct hyperplasia in the gland caused by infiltration of the tubular expansion.There is no uniform diagnosis criterion for this disease up to now,but it needs to be differentiated from idiopathic orbital inflammatory pseudotumor and Sj(o)gren syndrome in clinical diagnosis.The application of glucocorticoid is the primary treating regimen for benign lymphoepithelial lesion.Local radiation therapy and/or surgery extraction are available to patients unsensitive to glucocorticoid therapy.

Objective To discuss the feasibility of endoscopic axillary lymph node dissection by using balloon dilatation.Methods Seven patients with breast cancer from June 2005 to October 2005 were studied.Methylene blue injection around the areola or the tumor in combination with balloon placement in the axilla was performed to create surgical space for further endoscopic axillary lymph node dissection.Results The number of resected lymph nodes was 9~17(mean,12.3).No crushed lymph node was found.Positive lymph nodes were found in 4 patients.The operation time was 95~140 min(mean,114.3 min).The axillary vein and other important anatomic structures were clearly exposed.There was no edema of the upper limbs or other complications in the axillary area.No recurrence was noted during a follow-up survey for 2~6 months(mean,4.6 months).Conclusions Endoscopic axillary lymph node dissection can be performed after the establishment of surgical space by using balloon dilatation.

Dental Restoration, Permanent ; Humans ; Metal Ceramic Alloys ; adverse effects ; Nickel ; adverse effects

Adult ; Burnout, Professional ; Female ; Humans ; Jurisprudence ; Male ; Mental Health ; Middle Aged

Objective To establish a sensitive, simple and accurate HPLC-MS/MS method to quantify glycyrrhetic acid(glycyrrhetinic acid)in mice blood, and to further study pharmacokinetic profiles of glycyrrhetic acid after oral administration of glycyrrhizin and Bu- Zhong- Yi- Qi- Wan (BY). Methods Rats were intragastric administered of glycyrrhizin(glycyrrhizic acid, 61.5 mg/kg) and BY extract(3 g/kg, with the same mole of glycyrrhizin moiety), respectively. Plasma samples were collected after administration and extracted with liquid-liquid extraction, then by separated by liquid chromatography on a C8 reversion phase chromatographic column with gradient elution. Concentration of glycyrrhetic acid was detected by the validated HPLC-MS/MS. Non-compartmental pharmacokinetic profiles were constructed using the software of Das 2.0 software(Shanghai, China), and the pharmacokinetic parameters were compared using unpaired Student’s t-test. Results This bioanalytical method was fully validated and showed good linearity(r> 0.99), wide dynamic range(5-1000 ng/ml), and favorable accuracy and precision. Compared with the glycyrrhizin pure form group, BY significantly reduced the Cmax and AUC0-t of glycyrrhetic acid by 56% and 76%, respectively. Whereas no significant differences in Tmax, T1/2 and MRT were observed between the two groups. Conclusion The constituents in the BY prescription have significantly reduced the oral bioavailability of glycyrrhetic acid in rats than those in the glycyrrhizin pure form and the results indicate that some components in the BY have an inhibition effect on the absorption process of glycyrrhizin in the gut.