Objective　To examine the soluble protein patterns of three ordinary species of cockroaches in Anhui province by SDS-PAGE technique, and to find the method of scientific classification for cockroaches. Methods　The soluble protein liquid was made by the way of freezing and melting homogenate. SDS-PAGE was carried out in the liquid. After using Coomassie brilliant blue G250, the molecular weight of all of protein strips were measured and calculated by standard curve. Results　The numbers of female and male of Periplaneta fuliginosa were 31 strips and 29 strips; the numbers of female and male of Periplaneta Americana were 30 strips and 28strips; the numbers of female and male of Blattella germarica were 28 strips and 25 strips. The number and location of protein bands were different among these species. It was also obvious that the positions of the main protein bands in Blattella germarica were different from those in two Periplaneta species, and the positions of the main protein bands in female insect were different from those in male insect. Conclusion SDS-PAGE can be used as one of classification methods for cockroaches.

Relying on the R3 GF program,the present paper attempts to analyze the standards of multi-sectoral social participation projects design and implementation,to construct the key indicators and to explore the experience and bias,as well as to provide recommendations on how to improve performance and outcomes of small grant projects by multi-sectors.

BACKGROUND:Intratubal contraceptive devices are simple and effective, which can cause less interference on female reproductive function and endocrine balance.
OBJECTIVE:To explain the advantages and adverse effects of shape-memory intratubal contraceptive material and other intratubal contraceptive materials.
METHODS:A computer-based search of Wanfang, CNKI and PubMed was performed to search related articles concerning shape-memory intratubal contraceptive material and other intratubal contraceptive materials published from 1996 to 2013.
RESULTS AND CONCLUSION:The main intratubal contraceptive materials include memory metal biomaterials, non-degradable polymer biomaterials, and degradable polymer biomaterials. After implantation, the memory-shape intratubal contraceptive device plays a stimulating role in the tubal epithelium for a short time, but the effect on the mucosal epithelial layer of the fal opian tube is transient. With the increasing time of implantation, inflammatory reactions of the tubal epithelium relieve gradual y with repair of vil i on the tubal wal , indicating there is a reproductive tissue basis of fal opian tube recanalization. Polyethylene materials are characterized as high impact resistance, abrasion resistance, excellent stability to chemical drugs, water absorption, electric insulation, and bioinert, which are used as stent materials of almost al the intrauterine devices. Silicone is characterized as heat resistance, cold resistance, non-toxicity, resistance to biological aging, chemical stability, physical inertia, physical and mechanical properties, and silicone, after implantation into human body, cannot cause foreign body reaction. Polylactic acid has good chemical inertness, biocompatibility and ease of processing, and may be stil present in the body after 5 years of implantation.

Based on the development of clinical radiobiology and combined with teaching practice for recent years,we have preliminarily surveyed the teaching reform about this curriculum and proposed some advices about the clinical radiobiology teaching reform.

Objective To evaluate the changes in the expression of DJ-1 protein during myocardial ischemia-reperfusion (I/R) in diabetic rats.Methods Fifty male Sprague-Dawley rats,weighing 220-280 g,were used in this study.Type 1 diabetes mellitus was induced by intraperitoneal streptozotocin 65 mg/kg and confirmed by fasting blood glucose ＞ 16.7 mmol/L.Forty animals with type 1 diabetes mellitus were randomly divided into 3 groups:diabetes group (group D,n =10),diabetic sham operation group (group DS,n =15) and diabetic I/R group (group DIR,n =15).Another 10 non-diabetic rats in which citrate buffer 6 ml/kg was injected intraperitoneally were served as control group (group C).Myocardial I/R was produced by occlusion of the anterior descending branch of left coronary artery for 30 min followed by 120 min reperfusion in group I/R.At 120 min of reperfusion,5 rats were sacrificed and myocardial specimens were c(on)tained for determination of infarct size in groups DS and DIR,and 10 rats were sacrificed and myocardial specimens were obtained for microscopic examination and for determination of cell apoptosis,malondialdehyde (MDA) content,superoxide dismutase (SOD) activity and expression of DJ-1 and phosphatase and tensin homologue (PTEN) protein.Apoptotic index (AI) was calculated.Linear correlation between the expression of DJ-1 protein and MDA content,SOD activity,AI and expression of PTEN protein was analyzed.Results Compared with group DS,the myocardial infract size was significantly increased in group DIR (P ＜ 0.05).Compared with group C,MDA content and AI were significantly increased,SOD activity was decreased,the expression of DJ-1 was down-regulated,and the expression of PTEN protein was up-regulated in groups D,DS and DIR (P ＜ 0.05).Compared with groups D and DS,MDA content and AI were significantly increased,SOD activity was decreased,the expression of DJ-1 was down-regulated,and the expression of PTEN protein was up-regulated in group DIR (P ＜ 0.05).There was no significant difference in the parameters mentioned above between groups D and DS (P ＞ 0.05).There was linear correlation between the expression of DJ-1 protein and MDA content,SOD activity,AI and expression of PTEN protein and the correlation coefficients (r) were-0.734,0.593,-0.818,and-0.812 in turn.Conclusion Down-regulation of DJ-1 protein expression is involved in myocardial I/R injury in diabetic rats via decreasing anti-oxidative stress responses and upregulating PTEN protein expression.

Objective: To investigate the relationship between dietary -related behaviors of college students and their health , so as to provide the evidence for the health education for college students .Methods:A question-naire survey was conducted among 2105 college students from Xi′an Jiaotong University with stratified cluster ran-dom sampling .Results:In this survey , college students generally had unhealthy dietary behaviors , cognitive bia-ses of self-images and blind unhealthy weight -losing phenomenon as well .And there was a difference of dieting behaviors between college students of different sex and those of different body mass index .Conclusions:Unhealth-y dietary behaviors of college students were one of the main reasons leading to their abnormal BMI .Hence , it was necessary to improve health ethics education and dietary health guidance for college students , so as to introduce them to consciously assume their own health responsibilities and manage their health .

Objective To observe the change of neuron-specific enolase (NSE) an d glial fibrillary acidic protein (GFAP) in neonatal rats' cerebral cortex with hypoxic-ischemic brain damage (HIBD). Methods The 7 day- old SD rats were subjected to the ligation of right carotid artery, then were pu t into a hypoxic box to establish a HIBD model. The immunohistochemical method w as used to detect the expression of NSE and GFAP in rats' cerebral cortex. Results ① The NSE decreased in damaged cerebral cortex in HIBD 24 h group, after 7 days it gradually increased but was still lower than that of the controls. ② The expression of GFAP was limited and scarce in control and it did not change in HIBD 24 h group, while in HIBD 7 d group GFAP expression was increased and spread widely in the damaged cerebral cortex. Conclu sion ① The NSE decreases in damaged cerebral cortex in early stage of neonatal rat HIBD, suggesting that NSE is a specific marker for neuron damage . ② The GFAP increases in damaged cerebral cortex in the recovery stage of neon atal rat HIBD, suggesting that GFAP participates the repair of lesion region.

Objective To investigate the clinicopathologic features and the expression of p53,PCNA and Bcl-2in tricholemmal carcinoma.Methods Skin lesions were studied with HE staining and immunohis-tochemical markers for p53,PCNA and Bcl-2(S-P method)in22cases of skin tricholemmal carcinoma.Both morphological manifestations and immunohistochemical expression were observed.Results There were15fe-male and7male cases of tricholemmal carcinoma with the ages of40-79y,including16cases whose scalp were involved.Positive staining of p53,PCNA and Bcl-2was found in72%,100%and63%of patients,re-spectively.The positive cells of p53were diffusely distributed.PCNA-positive cells were increased along with the degree of differentiation.Significant differences of Bcl-2expression were found among the various degrees of differentiation(P

I sopropylmine vanillinate (IV) given iv 5 , 10 mg/kg in anesthe tized rats produced a dose-dependent decrease in mean arterial pressure and a concomitant decrease in heart rate.In pithed rats IV was unable to shift the phenylephrine doserespon-se curve to the right, but prazosine caused 53-fold shift.No direct effect of vasodilation was shown by IV on rabbit aorta strips .The concentration-response curve of isoprenaline in isolated rat right atrium was competitively inhibited by IV and propranolol. pA2 value was 4.0 for IV and 8.2 for propranolol. In addition, 'IV depressed contractility & heart rate of isolated rat atria.These results suggest that the hypotensive action of IV may be related to the decrease of HR, inhibition of contractility and slight blocking of B-adrenergic receptor. However the mechanism underlying the hypotensive effect of IV is to be further studied.