OBJECTIVE:To establish the quality standard of Zhizha jianwei granules.METHODS:TLC was used for the identification of Fructus Aurantii Immaturus and Fructus Crataegi.HPLC was applied for the determination of Hesperidin by which Diamonsil C18(200 mm?4.6 mm,5 ?m)chromatographic column was used and the mobile phase was acetonitrile-0.2%phosphoric acid(20∶80)with a flow rate of 1.0 mL?min-1.The column temperature was maintained at 40 ℃ and the detection wavelength was set at 283 nm.RESULTS:TLC method was proved to be specific and reproducible,and free from the interference of the negative control substance.The linear range of Hesperidin was 0.102～1.122 ?g(r=0.999 8)and its average recovery rate was 99.23%(RSD=0.20%,n=6).CONCLUSION:The established standard is suitable for the quality control of Zhizha jianwei granules.

Many moral choices occur in the contemporary biomedicine. With the development of science and technology along the moral choices encountered by biomedicine have been becoming more and more frequent and complicated with the emergence of unmerous new phenomena,In this paper, the historical background, the foundational features and the key principles of moral choices in the contemporary biomedicine are primarily discussed.

OBJECTIVE:To investigate the safety and efficacy of alanyl-glutamine dipeptide administered via peripheral vein total parenteral nutrition(TPN)in patients after gastrointestinal operation.METHODS:64cases after gastrointestinal operations were randomly divided into routine TPN group(control group)and TPN plus alanyl-glutamine dipeptide group(therapeautic group),all of which were treated with the corresponding medicines from the first day to the7th day after oper?ation.RESULTS:The levels of both seralbumin and prealbumin rebounded on the8th day after operation with those of the control group rebound more slowly,significant differences were noted between the2groups(P

0.05).CONCLUSIONS:Aztreonam is an effective and safe agent for treatment of cancer patients with lower respiratory tract infection.

Detection of minimal residual disease (MRD) exactly for lymphoma is important for estimating prognosis, assessing response, monitoring relapse and instructing treatment. Immunoglobulin (Ig)/T-cell receptor (TCR) gene rearrangements can be used as special tumorous markers for MRD testing by PCR.BIOMED-2 strategy is a method about multiplex PCR reaction designed by European Collaborative Organization. Monitoring MRD can be completed by real-time quantitative PCR. In this paper, the principle and research progress related to the application of IG/TCR in MRD detection are mainly reviewed for lymphoma in children.

Objective To explore the risk factors of shivering after intraspinal anesthesia.Methods 640 anesthesia recovery patients after intraspinal anesthesia from February 2011 to May 2015 were selected as the research subjects.According to whether patients appeared shivering,they were divided into research group and control group.The research group had 328 cases of appearing shivering,the control group had 312 cases without shivering. Basic clinical data of patients,operation time and blood loss,tympanic membrane temperature,anesthesia,anesthesia blocks plane indicators were compared to explore the risk factors of shivering after intraspinal anesthesia.Results This study included 640 cases,including 328 cases with chills reaction,the incidence rate was 51.25%.Single factor results showed that the low age,male,central cooler,surgery for a long time,the bleeding of more lumbar hemp,choice of anesthetic methods and anesthetic plane above T8 indicators, there were close relationship with shivering after intraspinal anesthesia,the differences between the two groups were statistically significant (χ2 =40.661,17.208, 32.369,32.931,36.624,14.844,26.84,all P<0.05).Multivariable logistic regression analysis results showed that age,gender,core body temperature,operation time,blood loss,spinal canal anesthesia methods and indexes of anes-thetic plane were important risk factors of chills reaction after anesthesia in patients with spinal canal anesthesia (OR=1.843,1.925,2.183,0.548,0.497,0.529,0.743,all P<0.05).Conclusion Male,younger age,operation for a long time,big intraoperative blood loss,tympanic membrane temperature low mode selection subarachnoid block anesthesia,and the anesthesia plane factor are the main risk factors of spinal canal anesthesia patients to appear chills response,the above factors are needed for close monitoring of patients with intraspinal anesthesia in the preoperative period.

Objective To discuss methods for recovery of bladder function and autonomous urination for patients with spastic bladder after spinal cord injury. Methods Patients in the experimental group(30 cases)received intermittent urethral catheterization 6 times per day,audio frequency electrotherapy and microwave therapy 2 times per day.The control group(30 cases)adopted routine urethral catheterization.The recovery of bladder function of the two groups Was observed.Results The rate of autonomous urination in the experimental group was higher than that of the control group(P＜0.01).Increase of bladder capacity and decrease of remnant urine volume in the experimental group was better than that of the control group(P＜0.05).Conclusions Intermittent urethral catheterization combined with audio frequency electrotherapy and microwave therapy could effectively promote the recovery of bladder function for patients with spastic bladder after spinal cord injury.

Objective. To detect vaginal mieroecosystem and discuss the importance to rectify the microeco- logical disturbance of vagina in gestational period in treating colpitis. Methods 250 prenatal care women in our hos- pital were selected as samples. We coUeeted vaginal secretion, made mieroeeological evaluation based on Gram stained smear, and detected leukocyte esterase, concentration of hydrogen dioxide and sialie acid-anhydride esterase. Different treatments were used randomly to these with vaginal ecological disturbance to investigate the cure rate of microeco- logical disturbance of vagina and colpitis,and the recrudescence rate of colpitis. Results 166 of the 250 women had microeeological disturbance of vagina,in the 166 women, 110 had semplice mieroecological disturbance of vagina and 56 had colpitis. In the group with vaginal ecological disturbance, the rate of well-balanced vaginal ecology was in- creased obviously and the rate of colpitiz was low. The difference was of statistical significance. In the group with col- pitis, after equilibrium treatment, the cure rate of colpitis in the study group was much higer than the control group after five-days' treatment,but no significant difference after ten-days' treatment. The recrudescence rate of colpitis was much lower in the study group than that in the control group. Conclusion Rectify the microecological distur- bance of vagina will shorten the period of treatment and reduce the recrudescence rate of colpitis.

Objective To discuss the influence of all- inclusive nursing for patients with orthopedic trauma on the aspects of their body and mind. Methods According to the related reference, made out allinclusive nursing ideas, and then used in 100 patients with orthopedic trauma (intervention group), routine rehabilitation was used in the control group (100 cases with orthopedic trauma). Compared the somatopsychic codition and the rehabilitation between the two groups. Results The 6 indexes of rehabilitation in the intervention group were significant better than those of in the control group. Somatopsychic condition in the intervention group was satisfactory. Conclusions All-inclusive nursing should be promoted in chnic by its merits.

Objective To explore the effect of yam polysaccharide for the diabetic mice. Methods Ninty mice were randomly divided into three groups ,yam polysaccharide group(n = 30), glibenclamide group (n = 30) and control group(n = 30). Mice were Intraperitoneal injection of alloxan (200mg/kg) to establish diabetic model. Yam polysaccharide group was administered yam polysaccharide for 12d. Results Yam polysaccharide significantly decreased the blood sugar of the diabetic mice. There were not significantly different in two groups (P ＞ 0.05).Conclusion Yam polysaccharide had obvious role in decreasing blood glucose.