Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Ocular Hypertension ; therapy ; Treatment Outcome

Objective:To clone the DNA sequence coding the F3T7,generate the fused protein F3T7,and investigate its function. Methods:We constructed prokaryotic expression vector pET32a(+)-F3T7 by subcloning technique,then fusion protein F3T7 was expressed and purified by affinity chromatography.and its activity of inhibiting the perliferation of HUVEC was assayed by MTT, then FITC was adopted to mark F3T7 to test its affinity with HUVEC,and its anti-angiogenses was detected by CAM. Results:We primarily certified that the fusion protein F3T7 selectively bound to HUVEC,inhibited its proliferation,and showed anti-angiogenic activity.Conclusion:F3T7 could have the abilities of binding to HUVEC and anti-angiogenesis

Objective To investigate the impact of Warfarin on posttraumatic pulmonary embolism patients .Methods 40 cases of posttraumatic pulmonary embolism patients from June 2008 to September 2012 in this hospital were collected as the treatment group ,40 healthy volunteers were chosen as the control group at the same time .Warfarin anticoagulant therapy was used for the treatment group ,no treatment was used in the control group .Monitoring the hemodynamic parameters of the treatment group at the time point of before treatment and 15 ,30 ,45 ,90 h after treatment .After treatment ,the hemodynamic parameters of the control group were used as control .Results Mean arterial pressure (MAP) ,cardiac index (CI) ,stroke volume (SV) ,stroke index (SI) and pulmonary artery wedge pressure (PAWP) of the treatment group were significantly lower than the control group before treatment with statistical significance (P<0 .05) .Heart rate (HR) ,systemic vascular resistance (SVR) ,pleural hydrostatic pressure (TFC) and pulmonary vascular resistance (PVR) were significantly higher that of control group ,with statistical significance (P<0 .05) . Before 45 h after the treatment ,MAP ,CI ,SV and SI of the treatment group were still significantly lower than the control group (P<0 .05) ,however ,there were no statistically difference after 90 h except PAWP (P>0 .05) .HR ,SVR and TFC of the treatment group were significantly higher than control after 90 h ,however ,the difference was not statistically significant of PVR in two groups .Conclusion Monitoring the hemodynamic changes during the treatment by Warfarin in posttraumatic pulmonary embolism patients can provide an important basis for the assessment and treatment of these diseases .

A wide range of underlying systemic factors have been reported in the setting of childhood stroke. Emerging research demonstrates that non-atherosclerotic intracerebral arteriopathies are the main risk factors. Minor infections may play a role in arteriopathies. Nonspecific presentation and doctor' s lacking awareness of pediatric stroke both contribute to initial misdiagnoses that result in delays in management. Acute management of children with stroke is primarily supportive including control of fever, blood pressure normalization to accepted age ranges, normalization of glucose, and maintenance of normal oxygenation, but there is no golden standard therapy at home and abroad. For secondary stroke prevention, the majority of children are treated with adult stroke therapeutic schedule. The prognosis of childhood stroke is not satisfactory. Many of these children are left with permanent neurologic deficits including injury of motor, sensory and cognitive defect and epilepsy.

With the obvious increasing of the survival of preterm infants in recent years,more and more attention to nutritional status has been attracted.Due to intrauterine undernutrition,preterm infants are on the demand of high calorie after birth,especially the very low and extremely low birth weight infants.However,simple gastrointestinal feeding often cannot meet the demand of nutrition and metabolism in these infants.Therefore,parenteral nutrition is considered to be an important method to obtain energy.Recently,application of large dose of intravenous amino acids during the early postnatal period has been widely used in foreign countries.It has been demonstrated that implementation of this strategy is not only safe,but also helpful on the improvement of preterm infants growth and nutritional status.Thus,establishment of parenteral nutritional support rapidly after birth is very necessary for preterm infants to catch up with intrauterine growth rate.

Objective To investigate the application effect of safety closed indwelling needle in pediatric transfusion.Methods A total of 500 cases from a pediatric respiratory ward were randomly divided into two groups:the Y-type indwelling needle group and the injury prevention group,each group contained 250 cases.The incidence of needle injury,success rate of puncture,length of indwelling,the condition of prolapse,obstruction and exosmosis were observed.Results The differences of the incidence of needle injury,success rate of puncture,length of indwelling,the condition of prolapse,obstruction and exosmosis in two groups were not statistically significant,the success rate of puncture depended on the vessel condition and the needle type,safety closed indwelling needle could completely avoid the incidence of needle injury.Conclusions Safety closed indwelling needle can avoid needle injury,which should be widely used in clinic.

Objective To explore the effect of Babu patch combined with Bufei-Zhengqi decoction on lung function and immunological indexes of pulmonary heart disease with Fei qi deficiency.Methods A total of 100 patients with pulmonary heart disease were randomly divided into study group and control group, 50 in each group. The control group was treated with routine therapy, the study group was treated with routine therapy and integrated Chinese medicine therapy (Babu patch combined with Bufei-Zhengqi decoction). Both groups were treated for 8 weeks. The effect of the two groups, and the lung function index, immunological indexes and adverse reaction were evaluated.Results The total effective rate in study group was 96.0% (48/50), the total effective rate in control group 84.0% (42/50), and there was significantly higher than that of the control group (χ2=8.130, P=0.043). After treatment, the lung function indexes FEV1 (65.26% ± 5.34%vs. 61.52% ± 6.21%,t=3.229), FEV1/FVC (67.59% ± 5.86%vs. 64.68% ± 5.52%,t=2.556) of study group were significantly higher than those of the control group (P<0.05). The IgA (2.37 ± 0.53 g/Lvs. 1.83 ± 0.76 g/L,t=4.121) in study group were significantly higher than the control group (P<0.01).Conclusions The application of Babu patch combined withBufei-Zhengqi decoction therapy, meet the requirements of treatment, improve lung function and immune function.

OBJECTIVE:To observe the clinical efficacy and safety of Cinobufacini capsule in the treatment of tumor patients with intractable hiccup. METHODS:120 tumor patients with intractable hiccup were randomly divided into observation group(60 cases) and control group (60 cases) according to the random number table. Control group received conventional treatment,treat-ment of primary disease,and the basic treatment of Methoxy metoclopramide tablets 10 mg,tid and intraorbital pressure on neural network method;observation group additionally received Cinobufacini capsule,2 capsules once,3 times a day. 1-week was regard-ed as a treatment course,and it lasted 4 courses. Clinical efficacy,Karnofsky functional status(KPS)score and disappearance time of hiccup were observed,and the incidence of adverse reactions was evaluated by TESS scale. RESULTS:After treatment,the to-tal effective rate in observation group was significantly higher than control group,the disappearance time of hiccup and TESS score were significantly shorter than control group,the differences were statistically significant(P<0.05). Before treatment,there was no significant difference in the KPS score;after treatment,the KPS score in 2 groups was significant higher than before,and observa-tion group was higher than control group,the difference was statistically significant(P<0.05). CONCLUSIONS:Both efficacy and safety of Cinobufacini capsule in the treatment of intractable hiccup are good,and it can shorten hiccup duration.

Objective To investigate the apoptosis of photoreceptor cells in experimental model of retinal detachment in mice. Methods Thirty-six adult C57Bl/6J mice were divided into 2 groups: retinal detachment model was set up in the left eyes of 18 mice by subretinal injection with 1.4% sodium hyaluronate in the experimental group, while the left eyes of other 18 mice underwent scleral puncture only as the control. The retinal sections were stained with histochemical and immunofluorescent staining and examined by confocal microscopy 1,3,7 and 28 days after injection. eye enucleated, and retinal sections studied by histochemistry, immunofluorescence labeling, and confocal microscopy. Rods, cones, and apoptotic cells were labeled by antibodies of anti-rod and anti-cone cells, and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL), respectively. Photoreceptor cell apoptosis and cell loss were assessed quantitatively by counting both surviving and apoptotic rod and cone cells. Results TUNEL-positive cells were only found in the outer nuclear layer (ONL) of the detached portion of the retina, which were detected at the 1st day after the detachment. The apoptosis of the cells reached the peak at the 3rd day and decreased sharply after 7 days. Photoreceptor cell loss of both rod and cone cells followed a similar time course after retinal detachment. Conclusion Apoptosis is a major pathological degeneration of photoreceptor cell death after retinal detachment.

0.05).However,there were significances between the two groups in HAMD scores at 1 and 2 weeks after treatment(P