Objective To observe the inhibition of different concentrations of curcumin on cell growth,and explore the molecular mechanism of curcumin on human osteosarcoma.Methods Curcumin of different concentrations(0,10,20 and 40?mol/L) was administered to U-2OS cells of human osteosarcoma for 12h,24h and 48h,respectively.The proliferation of U-2OS cells was measured by MTT assay.Results Curcumin significantly inhibited the proliferation of PC-3 cells in vitro in concentration-and time-dependent manners.The higher the concentration of curcumin,the stronger the inhibition was(10?mol/L,20?mol/L,40?mol/L of r=-0.9638,-0.9778 and-0.9764,respectively,P

Objective To investigate the hypothesis that food allergy in infants may be associated with variation in their intestinal microflora. The formation of intestinal microflora in healthy infants and changes in food allergic infants were detected.Methods 16S rRNA gene sequences specific for bifidobacterium, lactobacillus and escherichia coli in fecal were quantitatively detected by real-time PCR. The three fecal floras were assessed in 71 healthy infants and 100 infants with food allergy. Results After birth,there were bifidobacteria colonized in infantile intestine,then the number increased rapidly up to 5 times at the sixth month, which was always the preponderant flora. Lactobacilli was also presented in infantile intestine 1 month after birth and augment gradually. The number of Escherichia coli was less than bifidobacteria and lactobacilli and appeared to decline during the early infants. The number of bifidobacteria and lactobacilli in the infants with food allergy were markedly less than that in the healthy infants, but escherichia coli was significantly more than that in the healthy infants.Conclusions During the first year of life,the intestinal microflora in infants is in a developing process. Compared with the healthy infants,bifidobacteria and lactobacilli decrease, but escherichia coli increase in the food allergic infants.These results indicate that the probiotics may be benefit to the prevention and treatment of food allergy.

Animals ; Animals, Newborn ; Cells, Cultured ; Fibroblasts ; cytology ; metabolism ; Matrix Metalloproteinase 1 ; metabolism ; Matrix Metalloproteinase 2 ; metabolism ; Matrix Metalloproteinase 9 ; metabolism ; Myocardium ; cytology ; metabolism ; Oligopeptides ; physiology ; Rats ; Rats, Wistar ; Transforming Growth Factor beta ; pharmacology

Female ; Hamartoma ; Humans ; Infant, Newborn ; Mouth Diseases ; Palate, Soft

Acupuncture Therapy ; Adolescent ; Adult ; Ataxia ; congenital ; drug therapy ; therapy ; Child ; Combined Modality Therapy ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Male ; Moxibustion ; Spinal Cord Diseases ; congenital ; drug therapy ; therapy ; Young Adult

Mind map is an effective and intuitive knowledge organization and presentation tool which can help computer teaching commendably. We can use Mind Map to design and organize the whole teaching process, including preparing, designing, guiding, analyzing and reviewing. This way can turns tedious logic of computer teaching into imagery thinking and helps medical students improv-ing their learning effect of computer courses.

Objective To study the gene mutation characteristics of patients with spinocerebellar ataxias(SCA) in Ningxia region.Methods The SCA3/Machado-Joseph disease(MJD)trinucleotide CAG repeat number was detected by polymerase chain reaction(PCR),capillary gel electrophoresis(CGE) and DNA sequencing in 6 patients with SCAs and 4 normal kindreds from a family in Ningxia region.Results All patients and 1 kindred were positive for SCA3/MJD;other 3 kindreds were negative for SCA3/MJD.The CAG repeat number was expanded to 66～81 in the patients with SCA3/MJD and a kindred,and 22～33 in other 3 kindreds.Conclusion There is SCA3/MJD in the patients with SCAs of Ningxia region.Gene examining has the great significance for definite diagnosis in the patients with SCAs.

Objective To investigate the effect of indwelling double-J ureteral stents before flexible ureteroscope lithotrity in terms of operation time, postoperative hospitalization time and operation effect. Methods All clinical data of flexible ureteroscope lithotrity from 2009 to 2013 were reviewed. Operation time, postoperative hospitalization time and recurrence rate of calculus of patients who received operation directly and patients who received indwelling of double-J ureteral stents 2 weeks before operation were observed and compared. Results Op-eration time, postoperative hospitalization time, and recurrence rate of calculus of patients who received indwelling of double-J ureteral stents 2 weeks before receiving operation were obviously lower than patients who received operation directly. Conclusion Operation time, postoper-ative hospitalization time and recurrence rate of calculus can be decreased by indwelling double-J ureteral stents 2 weeks before operation.

Objective To explore the expression of G protein inwardly rectifier potassium channels subunit 2(GIRK*!2) in hippocampus of temporal epileptic rat.Methods After temporal lobe epilepsy was induced by kainic acid (KA), we took advantage of in situ hybridization to investigate the altered expression of GIRK*!2 mRNA in rat hippocampus.Results GIRK*!2 mRNA significantly increased in epileptic rats dentate gyrus region compared with normal control( P

Objective To analysis the clinical characteristics of kidney transplantation for the aged recipients and the influence of quadruple immunosuppressant on treatment result. Methods From 1990 to 2001,of 1251 cases of renal transpantation,the clinical data of 126 cases of the aged recipients over 60 years old were investigated.The aged recipients was divided into two groups;combination of cyclosporine(CsA),azathoprine(Aza) and corticosterioids was given in groupⅠ,In groupⅡ,except for CsA,mycophenolate mofetil(MMF),corticosteroids and Wu-T 3 quadruple therapy in the 47 cases aged recipients, Daclizumab or Basiliximab were injected in prophylaxis of acute rejection within 2 hours prior to transplantation and in someday after transplantation to the 24 aged recipients. The groupⅡ was compared with the groupⅠ in complications, the rate of acute rejection and the one-year-survival rate of patients/allograft.Results The rates of cardiocerebral vascular disease and infection complications in the aged recipients were higher than that in the non-elderly patients. In the groupsⅠ and Ⅱ, the postoperative complications was 74 55%and 38 03%, respectively;the rates of acute rejection were 12 73% and 4 23%,respectively;one year survival rate of patients/allografts was 81 82%/78 18% and 97 18%/95 77% respectively.Conclusions The cardiocerebral vascular disease and infection complications were the main factors affecting the outcome of renal transplantation in the aged recipients. The quadruple immunosuppressant regimens could reduce postoperative complications and acute rejection. At the same time one-year-survival rate of patients/ allografts have been improved in the aged patients.