2 weeks) (both, P

Child ; Echocardiography ; Humans ; Male ; Myocardial Infarction ; diagnosis ; Myocarditis ; diagnosis ; virology

Objective To study the influence of Captopril preconditioning on apoptosis and the expression of gp 130 in rats of acute myocardial injured. Method Wistar rats were randomly divided into three groups:control group, Iso group and Cap preconditioning group. Cardiomyocytes apoptosis was detected with TUNEL method. The expressions of Bcl-2 and Bax proteins in myocardium were tested by immunohistochemistry. The expression of gp 130 was tested with Western blot method. Results The index of cardiomyocytes apoptosis was decreased, the expressions of Bax proteins and gp 130 were decreased and the expression of Bcl-2 proteins was increased in Cap preconditioning group compared with Iso group (P<0.01 or P<0.05). Conclusion The expression of gp 130 could be decreased through Cap preconditioning, which can reduce the cardiomyocytes apoptosis.

Administration, Oral ; Anaphylaxis ; chemically induced ; physiopathology ; therapy ; Anti-Bacterial Agents ; administration & dosage ; adverse effects ; Blood Pressure ; drug effects ; Child ; Diarrhea ; drug therapy ; Humans ; Male ; Ofloxacin ; administration & dosage ; adverse effects ; Treatment Outcome

OBJECTIVE: To select and breed elite variety of Dendrobium officinale, thus to conserve the wild germplasms and promote the healthy developing of D. officinale industry. METHODS: System breeding method was employed for the breeding of new variety developed from wild germplasm oi Dendrobium officinale. Yield of fresh stems and leaves, quality characters including dried stem rate and raw polysaccharide content etc. were tested. ISSR molecular marker technique was used to distinguish genetic difference between new variety and other D. officinale materials. RESULTS: New elite variety 'Xianhu 2' was bred successfully with high polysaccharide content, fine adaptability, and high yield. 'Xianhu 2' had significantly higher yields of fresh stems-leaves and stems than D. officinale var. 'Yunnan Ruanjiao' and D. officinale var. 'Xianhu 1' and significantly higher dried stem ratio and raw polysaccharide content than 'Yunnan Ruanjiao' at 12, 24, and 36 m, respectively. ISSR analysis indicated that there were distinct differences in DNA level between 'Xianhu 2' and other D. officinale varieties and lines. D. officinale var. 'Xianhu 2' got its certificate for commerce releasing by Zhejiang Province in December 2011. CONCLUSION: 'Xianhu 2' is a new elite variety with good synthetic agronomic traits. Its breeding is beneficial for the cultivation of D. officinale.

Objective To explore a new model of medical humanity education in new situation. Methods Extensive survey was carried out among medical students,and discussion and consultation were hold to faculty and education administers.A new teaching model was established. Results A curriculum system with 1 or 2 core courses supported by some elective courses and social practice is under the process of implementation and achieved preliminary success.Conclusion The medical humanity education should pay attention to the development of connotation instead of pursuing the extension of quantity blindly,which means that students' school work must be eased by focusing on self-study and self-education.Medical students'life path with high-minded personality training should be strengthened.

This paper outlines kinds of index of eyes' optical effect and some different testing methods of intraocular lenses' optical quality.
Humans ; Lens Implantation, Intraocular ; Lenses, Intraocular ; Refractometry ; methods ; Sensitivity and Specificity ; Vision Tests ; methods

Objective To evaluate the effect of spleno-left adrenal vein shunt for the treatment of portal hypertensive upper GI bleeding caused by portal vein cavernous transformation in children.Methods Spleno-left adrenal vein shunt was performed in 8 children with portal hypertension due to cavernous transformation.The clinical data was reviewed.Results Portal vein pressure decreased significantly from (30±11)mm Hg to(22±7) mm Hg after shunt.There was no mortality perioperatively and during the follow-up.There were no recurrent hemorrhage nor hepatic encephalopathy occurring in the follow-up and all the children have normal intelligence and normal liver function though blood ammonia level increased significantly from(18±7)μmol/L to (60±17)μmol/L in 4 cases.In 7 cases in which preoperative whole blood cell count significantly decreased,the postoperative WBC,RBC,Hb and PLT was (7.64 ±4.46)×10~9/L,(4.54±0.97)×10~(12)/L,(133±5) g/L and (355.40±107.36)×10~9/L respectively (all P ＜0.05).In one case suffering from preoperative low PLT count the postop PLT reached 333×10~9/L,which was significantly higher than that preoperatively.Esophageal varices ameliorated in 6 cases.No stenosis of anastomotic stoma and thrombosis developed.Conclusion Spleno-left adrenal vein shunt is an effective procedure to treat portal vein cavernous transformation induced portal hypertension in children.

Objective To evaluate the perioperative safety and effect of irreversible electroporation (IRE) in the treatment of locally advanced pancreatic carcinoma (LAPC).Methods Twenty patients with imaging and cytohystological diagnosis of unresectable locally advanced pancreatic carcinoma were enrolled to undergo IRE treatment.The perioperative IRE-related complications were primarily analyzed to evaluate the safety of the procedure.The tumor reduction and biological response were analyzed through CT/MRI imaging and serous level of CA19-9.Results All patients were successfully treated with an average tumor size of(4.2 ± 0.6) cm and an average procedure time of (52.0 ± 23.3) minutes.Three intraoperative procedure-related complications were observed (15.0％) including two transient hypertension and one transient superventricular tachycardia.Six postoperative complications were described including two Grade A pancreatic fistula,one infection of incision,one portal vein thrombosis,one gastrointestinal hemorrhage and two functional delayed gastric emptying.The symptom remission rate was 73.7％ and lower serum CA19-9 level was recorded in all patients at discharge.Conclusions IRE is a safe and feasible procedure in the treatment of LAPC, and a reasonable operation strategy of IRE is helpful to consolidate the safety and efficacy.

Objective To explore the method of treatment on ventricular hemorrhage .Methods The clini-cal experience of 70 cases of ventricular hemorrhage with minimally invasive treatment of external ventricular drainage and injection of urokinase with CT stereotactic were summarized .Results Except for 3 cases of death ,67 cases sur-vived(95.7%),Grade Ⅰ-Ⅱwas 37 cases(52.9%),Grade Ⅲ was 21 cases(30.0%),Grade Ⅳ-Ⅴ was 9 cases (12.9%), and there was no complication of infection and bleeding associated with drainage among 70 cases. Conclusion The minimally invasive treatment of external ventricular drainage and injection of urokinase with CT ste -reotactic on ventricular hemorrhage improves the cure rate significantly ,reduces the mortality rate and the incidence of complications ,which has important clinical value .