Objective To apply simulator image workstation to making and verification of integrated blocks.Methods The outline of blocks drawn by doctors at simulator image workstation were transferred to the auto-cutting machine.The blocks were fabricated and were verified by the simulator.Then the qualified ones were verified by the linear accelerator.Results Of 1 371 blocks selected to verify by the simulator,1 107 (80.7%) blocks were qualified;Of 1 371 blocks qualified by the simulator,1 345(98.1%) blocks were qualified by the linear accelerator.Conclusion Simulator image workstation can improve efficiency and reduce the error in making blocks.

OBJECTIVE:To explore the effect of rehabilitation training on restenosis following stent implantation in patients with coronary atherosclerotic heart disease METHODS:A computer.based online search of VIP database was performed to search articles regarding restenosis following stent implantation and rehabilitation treatment for patients with coronary atherosclerotic heart disease,published between January 2000 and October 2009,with key words"coronary artery,stent implantation,intracoronary restenosis,rehabilitation exercise".The data were collected,and the references of each article were reviewed A total of 20 articles were included.RESULTS:Intracoronary stent has become an effective treatment for coronary atherosclerotic heart disease,and intracoronary restenosis has been focused in clinical studies.Prophase treatment following stent implantation is very important for coronary atherosclerotic heart disease.Rehabilitation exercise in combination with antiplatelet anticoagulatiOn and lipid treatment can reconstruct coronary arterial blood transport and significantly reduce incidence of restenosis and thrombus.CONCLUSION:Regular rehabilitation exercise following stent implantation can ameliorate degree of vascular restenosis.

OBJECTIVE:To establish a dualwavelength TLC-scanning method for the determination of nucleosides in the preparation of Cordyceps sinensis.METHODS: The sample was analyzed on silica GF254 thin layer plate using 1% CMC-Na as adhesive and chloroform-ethylacetate-isopropanol-water-ammonia(8∶2∶6∶0.5∶0.12) as developing agent and detected by dualwavelength reflection sawtooth scanning(?s=254 nm and ?R=300 nm).RESULTS:Adenosine,Uracil and Uridnine had good linearity in the ranges of 11.16～58.03 ?g(r= 0.998 5),1.04～6.24 ?g(r=0.991 5) and 0.8～4.8 ?g(r=0.991 2) respectively,and their average recovery rates were 95.559%,96.366% and 95.270% and RSD were 0.74%,0.73% and 0.98% respectively(n=6).CONCLUSION: The method is simple,accurate,reproducible,and can be used as the quality control of Cordyceps sinensis preparation.

ABSTRACT:Objective To investigate the effects of poly ADP-ribose polymerase (PARP ) inhibitor 3-aminobenzene (3-AB)on the delayed development cerebral vasospasm (DCVS)after subarachnoid hemorrhage (SAH)and on the inflammatory factors,namely monocyte chemotactic protein 1 (MCP-1 )and hypersensitive c-reactive protein (hsCRP),and to explore the relationship between these and the signaling pathway of NF-kappa B (NF-κB).Methods Eighty male Sprague-Dawley rats were randomly divided into four groups:normal group (n =8),sham-operation group (n =8),SAH model group (n =32)and 3-AB group (n =32).We established 64 SAH model animals by double injection of blood into the cisterna magna.Half of the SAH model animals were treated with 3-AB by intraperitoneal injection (30 mg/kg).These rats were killed to obtain specimens respectively at days 3, 5,7 and 14 after the second blood injection.The morphological changes of basilar arteries were observed under the light microscope.The contents of PARP,MCP-1 and hsCRP in brain tissues were detected with enzyme-linked immunosorbent assay (ELISA).The expression of NF-κB in basilar arteries was determined by immunohistochemistry.
Results Compared with those in the sham-operation group,the degree of basilar artery spasm reached the peak [(30.47±3.89)%]at day 5 after established SAH model;the thickness and diameter of basilar artery were (1 6.44 ±1.32)μm and (1 78.21 ± 1 1.13)μm,respectively.Cerebral blood flow was reduced by nearly 60% (P <0.01 ). The expression of NF-κB in the cytoplasm and nucleus and PARP content in brain tissue were both increased significantly (P < 0.01 ).MCP-1 [(365.29 ± 28.08 )pg/mL ] and hsCRP [(402.1 6 ± 48.99 )ng/mL ] were significantly enhanced (P <0.01).Compared with the SAH group,after 5 days’intervention with 3-AB,there was obvious alleviation in the spasm degree of basilar artery [(22.65±3.21)%],the thickness [(14.89±1.27)μm]and diameter [(1 98.56±10.91)μm],respectively (P <0.01).Cerebral blood flow was significantly enhanced,but the expression of NF-κB in the cytoplasm and nucleus was decreased and PARP in brain tissue was significantly decreased (P < 0.01 ).MCP-1 [(126.5 1 ± 18.67 )pg/mL]and hsCRP [(285.39 ± 39.07 )ng/mL]in brain tissue were significantly declined,respectively (P <0.01).Conclusion PARP inhibitor 3-AB can alleviate DCVS and inhibit the inflammatory response in brain tissue after SAH.The mechanism may be related to NF-κB signaling pathway.

Objective To probe into the methods of postoperative nursing care for the patient undergoing domino liver transplantation.Methods The key of postoperative care included:monitoring of vital signs,the condition of cruor and the state of drainage rigorously; keeping the balance of intake and output; taking the immunosuppressive drugs correctly; proper diet; prevention of complication;giving psychological nursing.Results The patients passed the perioperative period successfully and were all cured.Conclusions To aim at the characteristic of this operation,implement holistic nursing care,is an important link to assure the patients of domino liver transplantation to recover smoothly after the operation.

Objective:To evaluate the clinical efficacy and safety of Tongqiao Biyan granules in the treatment of sinusitis. Meth-ods:Totally 240 cases of sinusitis were randomly divided into two groups, each group had 120 cases. The patients in observation group were treated with 1 bag of Tongqiao Biyuan granules, 3 times a day, while the patients in control group were treated with busesonide nasal spray 100 μg each side nasal cavity, once a day. After 3 weeks,curative effect and adverse drug reaction of the two groups were observed. Results:After the treatment, nasal congestion, runny nose, headache, cough and other signs and symptoms were improved more significantly in the observation group than those in the control group (P<0. 05), the total effective rate of the observation group was 92. 5%, while that of the control group was 79. 2%, the difference was significant (P<0. 05). During the treatment, the two groups showed no obvous adverse drug reactions. Conclusion: The effect of Tongqiao Biyuan granules in the treatment of sinusitis is significant with no obvious adverse drug reactions, which should be recommended to use in clinic.

0.05).

Vulnerable plaque (VP) has been widely considered as the main cause of acute coronary syndrome (ACS) and cardiac death .In recent years ,invasive or non-invasive intravascular ultrasound imaging has become one of main measures for detecting VP .The present article made following overview on advantages and disadvantages of intravascular ultrasound imaging assessing VP .

As an efficient gene transfer vehicle lentiviral vector has been widely used in therapy research. Comparing with other retrovirus vectors, lentiviral vectors have the unique ablility of transfecting nondividing cells and terminal differentiated cells. In addition, lentiviral vectors can accommodate two or more promoters and can carry larger foreign gene insertions. Now the new generation of lentiviral vectors encoding transcriptional control sequence provides effective means for the regulation of foreign gene expression. The development of lentiviral vectors and its application in the gene therapy field were summarized.

BACKGROUND: Previous studies have proved that stress can affect immunity function and disturb homeostasis of bodies. Moderate exercise can alleviate the psychological stress reaction to some degree.OBJECTIVE: To observe the effects of moderate exercise on lymphocyte number in spleen and mesenteric lymph nod,interferon-y (IFN-γ) and interleukine-4(IL-4) level in serum, as well as Th1/Th2 balance in rats under chronic psychological stress.DESIGN, TIME AND SETTING: A randomized control animal experiment was carried out in the laboratory of Sports Human Science in Chengdu Sports University from May to October in 2007.MATERIALS: A total of 40 healthy male SD rats were divided by random digits table into 4 groups: control group, psychological stress group, exercise group and exercise+psychological stress group, with 10 ones in each group.METHODS: Before experiment, rats in exercise group and exercise+psychological stress group underwent 3 days of adaptive swimming training, 30 min/(t·d). During experiment, they were made swimming with no weight loading for 8 weeks, 60 min/(t·d).From day 2 post exercise on, rats in exercise+psychological stress group began to undergo the 21 days of chronic restraint stress,6 hours per day. At the same time, the chronic restraint stress models were established in the psychological stress group. Rats in the exercise group were fed for 22 days since the 8~(th) day of exercise ended. Rats in the control group were only fed without any intervention.MAIN OUTCOME MEASURES: The lymphocyte number in spleen and mesenteric lymph node was measured, and ELISA was employed to measure IFN-y and IL-4 levels as well as the ratio of IFN-y/IL-4 in serum.RESULTS: Compared with the control group, the lymphocyte number, the level of IFN-y and the ratio of IFN-y/IL-4 were significantly decreased in psychological stress group (P < 0.01 ), and the Thlf'rh2 balance was broken. Compared with the psychological group, the exercise+psychological stress group had got significantly higher lymphocyte number, IFN-γ level and ratio of IFN-γ/IL-4 (P<0.05). The lymphocyte number in spleen and mesenteric lymph node in the exercise group showed not a difference of significance compared with the control group, but a tendency towards going up. There was no difference of significance in IFN-γ level and ratio of IFN-γ/IL-4 among the exercise+psychological stress group, the exercise group and the control group.CONCLUSION: Moderate exercise can increase lymphocyte number in spleen and mesenteric lymph node significantly,up-regulate the IFN-γ level so as to regulate the disbalance of Th1/Th2, and maintain immunity homeostasis.