This article briefly introduces the integrated management of hospital medical equipment after selection, order and approval.The importance of continuous education for technical staff is also disscussed.

The properties of the corticosterone receptors and the effects of para-chlorophenylalanine(pCPA) on the binding capacity of the corticosterone receptors of the hippocampal cytosol in adrenalectomized rats were investigated with receptor radioligand binding assay(RLA) using (3H)-corticosterone as the ligand. It was fonud that the corticosterone receptors possessed the properties of satu-rability, limited binding capacity, high affinity and high specificity. RLA demonstrated a linear Scatchard plot, which implies that there is only one receptor or one group of receptors with similar Kd values. Four days after intracerebroven-tricular injection of 1 mg of pCPA, the hippocampal concentration of 5-HT was markedly decreased, and the binding capacity of the cytosol receptors was increased, which implies that the functional activity of the serotoninergic system in the hippocampus is an important factor in the control of the number of corticosterone receptors in rats.

OBJECTIVE: To investigate the effect of n-butanol extract of Morindae officinalis How(BEM) on apoptosis of myocardial cells in neonatal rats undergoing anoxia/reoxygenation(A/R).METHODS: The rats were divided into 6 groups: normal group,A/R model,Danshen ingection(SM),BEM high,middle,low groups.The morphological changes of the apoptotic myocardial cells were observed using electron microscope.The apoptotic index was measured by in situ nick end labeling(TUNEL).The expression of Bcl-2 and the apoptotic protein Bax were detected by immunohistochemical staining.RESULTS: Morphocytology revealed the remarkable protective effect of BEM against apoptosis of myocardial cells during A/R.TUNEL test showed significantly lower apoptotic indexes in SM,BEM high,middle,low groups than in A/R group,and in all the SM,BEM high,middle,low groups,the positive expressed Bcl-2 gray scale value increased significantly(P

Clinical respirator is the most commonly used first-aid life-support equipment.Therefore,to strengthen the maintenance and management of respirator is very important for the enhancement of its safety and availability and treatment efficacy.

Objective To explore the curative effect of bisoprolol fumarate combined with wenxin granule for patients with heart failure complicated with premature ventricular.Methods 132 patients with heart failure and premature ventricular complexes were enrolled in our hospital from January 2013 to January 2016,and which patients divided into two groups,bisoprolol fumarate group (Group BF,n =66) accepted bisoprolol fumarate treatment (5 mg/time,1 times/day),and bisoprolol fumarate combined with wenxin granule group (Group BW n =66) adopted wenxin granule (9 g/time,3 times/day) based on the patients in Group BF.After 4 weeks treatment,the curative effect of all patients were evaluated with electrocardiogram monitor and cardiac ultrasound examination,and the plasma NT-proBNP concentration and hemorheology indexes were analyzed compared with pre-treatment.Results The recovery of ventricular function in Group BW was better significantly than that patients in Group BF from the cardiac ultrasound examination (P ＜ 0.05);The total relieved effective of clinical symptom,premature ventricular complexes and heart failure in Group BW were more remarkable than those in Group BF,and which difference from them was significance (P ＜ 0.05);The blood viscosity,plasma viscosity,plasma fibrinogen and plasma NT-proBNP concentration in patients with Group BW were significantly lower than those Group BF (P ＜ 0.05).Conclusions The clinical curative effect of bisoprolol fumarate combined with Wenxin Granule for heart failure complicated with premature ventricular was more remarkable compare with bisoprolol fumarate single treatment,and which possessed the relative safety.

Objective To investigate the clinical effect of adopting B-type ultrasound guided peripherally inserted central catheter (PICC) in the patients with lung cancer.Methods Two hundreds and ninety-five cases of lung cancer undergoing PICC in our hospital from January 2011 to May 2016 were selected as the research subjects and divided into the observation group (151 cases) and control group (144 cases) according to the PICC methods.PICC in the observation group adopted the B-type guided modified Seldinger technique,while the control group adopted the conventional method.The venous thrombus occurrence situation in the two groups was comparatively analyzed.Results (1) The platelet count (PLT),plasma D dimer (D-D) and thromboxane B2 (TXB2),endothelin (ET),6-keto-prostaglandin F1 alpha (6-keto-PGF1α) before PICC had no signigicant difference between the two groups (P>0.05).PLT,D-D,TXB2 and ET at 12,24 h after PICC in the observation group and control group were gradually increased,while plasma 6-keto-PGF1α was gradually decreased,the intra-group difference among different time points had statistical significant (P<0.05).PLT and plasma D-D,TXB2 and ET in the observation group were significantly lower than those in the control group,while keto-PGF1α was significantly higher than that in the control group,the difference between the two groups was statistically significant(P<0.05).(2)The incidence rate of venous thrombosis in the observation group was significantly lower than that in the control group,the difference was statistically significant between the two groups (P<0.05).Conclusion Adopting B-type ultrasound guided modified Seldinger technique for PICC in the patients with lung cancer has much lower incidence rate of venous thrombosis.

OBJECTIVE To study the classification and distribution of the ?-lactamases produced by Escherichia coli in diabetic patients.METHODS To detect the ?-lactamases produced by E.coli in diabetic patients with a multi-disk synergy test.RESULTS Forty-nine strains of 60 strains in our study produced the ?-lactamases with total positive rate of 81.67%,From them 5 strains were producing penicillinases,17 strains producing BSBLs(28.33%),and 27 strains were producing ESBLs(45.00%),no carbapenem-hydrolyzing ?-lactamases were detected.In 27 strains producing ESBLs,the sensitivities to the ceftazidime,aztreonam and cefotaxime were similar.CONCLUSIONS The types of ?-lactamases produced by E.coli in diabetic patients are mainly BSBLs and ESBLs,without carbapenem-hydrolyzing ?-lactamases;the sensitivities to the ceftazidime,aztreonam and cefotaxime are similar.

Objective: To search for a new method for establishing high precision 3D finite element model of maxillary anterior dentition segment. Methods: Spiral CT (SCT) scanning was used to obtain the data of digital imaging and communications in medicine (DICOM) of teeth and the periodontal tissues; the data were used to construct the iges NURBS model by combining converse engineering software Geomagic and computer-aided design (CAD). The 3D finite element analysis model was created after the unit lattice was meshed and checked by Hypermesh pre-processors. Results and Conclusion: We have successfully established the high quality 3D model of maxillary anterior dentition segment and the periodontal tissues (including periodontal ligament and alveolar bone); the model contained 640 396 tetrahedrons and 117 889 nodes. The present method is easy-to-use and is suitable for establishing the model for complex tissues like teeth and periodontal tissues. The established model has a high comparability and provides a good basis for studying the biomechanic behavior of maxillary anterior dentition segment under different orthodontic force systems.

AIM To investigate the effects of tetracycline on the expression of adhesive molecules E selectin and intercellular adhesion molecule 1 (ICAM 1) on human umbilical vein endothelial cells (HUVECs) stimulated by tumor necrosis factor alpha (TNF ?) or lipopolysaccharide (LPS). METHODS HUVECs were activated by TNF ? (0 5～50 ?g?L 1 ) or LPS (0 5～50 mg?L -1 ). Tetracycline (10 -6 ～10 -4 mol?L -1 ) was used to incubate HUVECs for 1 h before stimulation with TNF ? ( 1 ?g?L -1 ) or LPS ( 5 mg?L -1 ) and co incubate HUVECs with TNF ? or LPS for 4 h or 18 h. The expression of adhesive molecules on HUVECs was measured by a cellular enzyme linked immunoabsorbent assay (Cell ELISA) method. RESULTS After stimulation of HUVECs with TNF ? or LPS for 4 h induced E selectin expression, and exposure HUVECs to TNF ? or LPS for 18 h increased constitutive expression of ICAM 1 compared with control group ( P