Adolescent ; Age Factors ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Risk Factors ; Tachycardia, Supraventricular ; etiology ; pathology

Objective To study how polyclonal antibodies against ciliary neurotrophic factor (CNTF) reduce botulinum toxin-in- duced axonal sprouting and affect paralytic muscle.Design Experimental study.Participants 20 New Zealand rabbits.Methods In 10 rabbits randomly selected,left superior rectus were as control (Group 1),right superior rectus were injected with botulinum toxin A (BTXA) 2.5U (Group 3).At 14th day,bilateral superior recti were taken out under general anaesthesia.In the other 10 rabbits,left su- perior recti were injected equivalent physical saline (NS) as control (Group 2);right superior recti were injected with BTXA 2.5U,after 4 days,injected with 50?l polyclonal antibodies against CNTF at same site (Group 4).And at 14th day,bilateral superior recti were taken out for electron microscopy,dyed with acetylcholinesterase,argentums (Ag) to show nerve axonal sprout,and accounted and mea- sured with Leica microsystems.Main Outcome Measures The mean number of sprouts and the mean total length of sprouts,and the uhrastructure change of muscle by electron microscopy.Results In Group 1,the mean number of sprouts and the mean total length of sprouts were 5.75% and 10.53?m respectively;Group 2 were 6.11% and 11.16?m;Group 3 were 84.04% and 170.71?m;and Group 4 were 54.77% and 68.12?m.The differences were statistically significant (all P=0.000).Electron microscopy showed that after admin- istration BTXA alone (Group 3),muscle atrophied obviously,nerve myelin sheath increased,while the structure of nerve and muscle re- main invariable.The Group 4 showed that local myofilament disrupted and dissolved,degenerative myocytes necrotized and disintegrat- ed into fragments,which led to partial unreversible destroy.Conclusion Polyclonal anti-CNTF can reduce BTXA-induced axonal sprout- ing,lead to partial unreversible destroy of muscle,which may prolong the time of paralytic muscle resuming.It suggests that polyclonal anti-CNTF could prolong the duration of muscle paralyses induced by botulinum toxin.

AlM:To explore the clinical effects of using rigid gas permeable contact lens ( RGP ) for refractoriness amblyopia patients.
METHODS: Ninety - eight cases ( 98 eyes ) were voluntarily divided into RGP group and frame glasses group, and the two groups were received the regularity combined training to treat amblyopia for 6mo. We overviewed the corrected vision (on that day, 1, 3, 6mo) and the complication in RGP group .
RESULTS: The corrected vision in RGP group was obviously better than that in control group during the same time. The therapeutic efficacy in RGP group was better than that in frame glasses group, without serious complications at 6mo after treatment.
CONCLUSlON: RGP groups could get better corrected visual acuity. lt is safe and effective to improve corrected vision for refractoriness amblyopia patients.

Objective:To prepare the canine multivalence serum for emergent prevation and theatmentor canines infection disease.Methods:Selecting 20 healthy Kunming dog,which is more than one years old.The canines were immuned by regulary with Canine distemper virus(CDV),Canine parvovirus virus(CPV),Infectious canine hepatitis virus(ICHV),Canine coronavirus(CCV),Canine parainflu-enza virus(CPIV).The blood were draw in freedom germ from neck's artery at canines and then blood was centrifugate for isolating serum.To check antibody's tite, micro serum neutrolization(SN) and hemagglutination inhibition(HI) were used.Results:Canine multivalence serum is successfully preparation and good curative effects in clinic.Conclusion:Canine multivalence serum may be of great value in economics and society and contribute to curative disease.

Microbes are important composition of ecology system. Nowadays it's very important to study the microbial diversity and community structure, especially in water bioremediation by microbiological resources. Modern molecular biology techniques provide effective methods to study the microbial ecosystem in aqua. Several techniques were summarized, including 16S rDNA clone library, DGGE (Denaturing gradient gel electrophoresis), RFLP (Restriction Fragment Length Polymorphism) and T-RFLP (Terminal Restriction Fragment Length Polymorphism), while their application to analyze water microbial diversity were introduced.

Her-2 receptor has already been identified as one of the most important biological markers of some malignant tumors,and also plays an important role in the biological behavior of those tumor cells.The over-expression of Her-2 receptor is associated with radioresistance of various tumor cells,and thus an antibody to Her-2/neu receptor can probably function as a radiosensitizer.In this review,we summarized some advances in the molecular mechanism and clinical aspects of the relationship between Her-2 and cellular radiosensitivity.

Objective To explore the therapeutic effect of focused ultrasound(FU)on chronic cervicitis unitarily and its influential factors.Methods Three hundred and thirteen patients of chronic cervicitis were treated with the device of CZF model focused ultrasound.The therapeutic effects in 300 cases with complete records,as well as the relationship between the diameter of cervical erosion and the exposure time to FU were analyzed.Results The effective rates of FU in treatment of chronic cervicitis unitarily were 100.0%(53/53),95.2%(120/126)and 91.7%(111/121),respectively,for mild,moderate and severe cases.The overall effective rate was 94.7%(284/300).With other parameters of FU unchanged,the exposure time to FU showed an exponential relationship with the diameter of erosion,and the regression equation was D=31.71e~(0.4808t)(R~2=0.9966).Conclusions The controllability and achievement ratio of FU to treat cervical erosion could be enhanced when exposure time and manners were fully considered based on the diameter and type of cervical erosion.A satisfactory therapeutic efficacy could be achieved with FU to treat cervical erosion unitarily.

AIM: To observe the neovascularization process with no intervention in rat allogenic penetrating keratoplasty. METHODS: Allogenic penetrating keratoplasties were successfully performed in 34 female SPF SD rats with no intervention after operations. Corneal neovascularization(CNV) process was noted on day 4, 7, 15 and 30 with operating microscope. The vascular area surface was calculated using the formula C/12×3.14×[r2-(r-I)2].RESULTS: CNV was noted in 29 out of 34 rats (85%). Firstly, the new vessels distributed around the cornea like a brush then gradually extended towards the center. The vessels were distorted and massive with branched tails, they continued growing to reticulated veins in peak time then gradually atrophied. The average neovascularization area (SE) on day 4, 7, 15 and 30 was 11.8±3.5mm2, 18.5±4.0mm2,14.4±4.3mm2 and 6.0±1.8mm2 respectively and 12.7±1.9mm2 in total. The average percentage that new vessels accounting the whole cornea area(SE) was 30.8%±8.7%, 65.3%±12.8%, 59.4%±14.5%,36.2%±10.9% and 48.7%±6.4% in total.CONCLUSION: In rat allogenic penetrating keratoplasties without intervention, CNV presented on day 4 and reached the maximum area on day 7. Then the vessels gradually atrophied, about 50% of the maximum area still remained on day 30.

Background Experimental study showed that heparanase (HPA)is overexpressed in choroidal neovascularization,suggesting that it may play a role in the pathogenesis of angiogenesis.To certify HPA inhibitor suppress the formation and development of new blood vessel has an important significance for the treatment of choroidal neovascularization.Objective The aim of this study was to investigate the effect of HPA inhibitor on the proliferation of human umbilical vein vascular endothelial cell (UVEC) and the expression of HPA.Methods Hunan UVEC was primarily cultured and passaged and the third generation cells were used in the experiment.Phosphomannopentaose sulfate (PI-88) solution,a HPA inhibitor,was prepared with endothelial cell medium and the end concentrations were 400.00,200.00,100.00,50.00,25.00,12.50,6.25 mg/L respectively.The cells were treated with PI-88 solutions for 24,48 and 72 hours.The growth and proliferation of human UVEC were analyzed using MTT colorimetric assay at absorbance 570 nm.The expression of HPA in the cells was detected by immunochemistry in 48 hours after addition of PI-88.Results Cultured human UVEC showed the fusiform and polygon in shape.The A570 values of human CVEC were significantly different among various concentrations of PI-88 groups (F=2.721,P=0.053 ) and different action time (F=9.656,P =0.002).When PI-88 was administered for 24 hours,the A570 values of human UVEC were insignificantly altered in comparison with the one without PI-88 culture group (P＞0.05 ).However,in 72 hours after experiment,the A570 values were significantly declined as the PI-88 concentration was ＞50.00 mg/L ( P＜0.05 ).When PI-88 was administered for 48 and 72 hours,the A570 values of human UVEC were significantly higher than those of 24 hours in ＜50.00 mg/L groups (P＜0.01 ),but no statistical differences were seen in ＞100.00 mg/L groups among various time points (P＞0.05 ).HPA was intently expressed in the cytoplasm of human UVEC.However,at 48 hours after addition of ＞25.00 mg/L PI-88,the HPA expression was obviously weaker.Conclusions PI-88 can suppress the growth and proliferation of human UVEC at the dose-and time-dependent manner by downregulating the expression of HPA in the cells.