Diagnostic Errors ; Humans ; Myoclonus ; diagnosis ; Tics ; diagnosis

Objective:To prepare and identify the monoclonal antibody against hPPAR?2.Methods:cDNA of hPPAR?2 was obtained from pMD18-T/hPPAR?2.hPPAR?2 was expressed in prokaryocyte and purified successfully.The anti-hPPAR?2 monoclonal antibodies (mAbs) 1B4,3H2,3H10,10D6 and 10D8 were produced by immunization with purified hPPAR?2.The specificity of the antibodies was identified by ELISA,Western blot assay and immunocytochemistry.Results:Five novel murine monoclonal antibodies only against hPPAR?2 were harvested,and they were all specific to hPPAR?2.Conclusion:The antibodies obtained provide more useful tools for following research and settling the basis for screening new drugs and mechanism.

Through the research and practice of computer basic course at medical colleges, the paper summarizes the experience of using modern technical means, to reform the traditional teaching ideas, teaching methods, teaching means and teaching management, to diversify teaching resources sharing. It also analyzes the shortcomings and makes recommendations to further strengthen the course development.

Objective TostudytheextrahepaticinfectionandreplicationofhepatitisCvirusinthe patientswithhepatitisC .Methods HCVRNA ,intermediateofHCVreplication(minus strandofHCV RNA) ,andHCVantigensweredetectedbyreverse transcriptasepolymerasechainreaction(RT PCR) ,in situhybridization(ISH)andimmunohistochemistryin 38autopsyextrahepatictissuespecimens(including kidney ,heart,pancreas ,intestine ,adrenalgland ,spleen ,lymphnode,andgallbladder)from 9hepatitisC patients.Results ByRT PCR ,all 9patientswerepositiveforHCVRNAinkidney ,heart ,pancreas , andintestine .However,only 6 (6 6 .7% ) patientswerepositiveforintermediateofHCVreplication .The positiveratesofHCVRNAandHCVantigensinextrahepaticorgansotherthanspleenwere 5 5 .6 % (5 pa tients)and 6 6 .7% (6 patients)respectively .HCVRNAandHCVantigenswerepositiveinthefollowing cells:myocardialcells,epithelialcellsofintestine ,interstitialcellsofkidney ,epithelialcellsoftubulesand glomerulus ,pancreasacinarcellsandepithelialcellsofpancreaticduct,epithelialcellsofmucousmembrane sinusofgallbladder ,cortexandmedullacellsinadrenalgland ,andmononuclearcellsinlymphnode .Con clusion TheseresultsshowthatHCVreplicationexistsinvariousextraheptictissuecellsatlow level, whichmayhavecertainpathogeneticandclinicalsignificanceinthepatientswithHCVinfection .

Objective To investigate the negative effect of cigaratte smoking on periodontal theraphy. Mothods 84 patients with periodontopathy were selected and divided into two groups. The smoking group (group A) included 33 patients and the non-smoking group (group B) included 51 patients. All the patients received a systematic treatment. The effects of the treatment between different groups were compared through the debris index(DI),calculus index(CI),periodotal disease index(PDI) which were respectively recorded before and after treatment.Results After the treatment the DI,CI,PDI of two groups were all decreased; wheras compared with group A, every index of group B was significantly lower. Conclusions Somking seriously influences the effect of periodontal therapy. Therefore, to give up smoking is very important for preventing and treating periodontopathy.

Equipment Failure ; Female ; Heart Ventricles ; pathology ; surgery ; Humans ; Middle Aged ; Pacemaker, Artificial

Objective To observe the effects of myriocin(ISP-1) on improvement of glomerular messangial cell(GMC) apoptosis and on gene expression profiles of cell cycle regulatory proteins in GMC.Methods Rat GMC was cultured with 100 ?mol/L ISP-1 for 6,12,24,and 48 h,apoptosis was evaluated through flow cytometry,Hoechst33258/PI fluorescence stainig and DNA fragmentation analysis was carried out under Sepharose electrophoresis to observe the changes of apoptosis and DNA fragmentation.Gene expression profiles of cell cycle regulatory proteins were detected by SuperArray Real-Time PCR microarray analysis.The expression of Bax and Bcl-2 proteins was investigated by Western blotting.Results ISP-1 could significantly induce GMC apoptosis in a time-dependent manner,which was the most significant after 48 h.Apoptosis bodys of GMC were observed by Hoechst/PI fluorescence staining,and a typical ladder pattern was identified in DNA electrophoresis.SuperArray Real-Time PCR microarray analysis revealed that ISP-1 could up-regulate the expression of genes involved in DNA damage,apoptosis and cell cycle regulation,such as Rad51,Atm,Brcal,caspases,cyclinA2,cyclinC,chek1,cyclinB1,cyclinB2,cyclinD2,cyclinF,Cdc25a,and P27.Western blotting showed that ISP-1 could significantly up-regulate Bax protein expression and down-regulate Bcl-2 protein expression,respectively.ConclusionISP-1 could induce GMC apoptosis in a time-dependent manner,propabably through influencing gene expression of cell cycle regulatory proteins and apoptosis proteins.

Objective To investigate the values of CD21 and CD43 proteins in the differential diagnosis of mucosa-associated marginal zone B-cell lymphoma (MALToma) from benign lymphadenosis. Methods The expression of CD21 and CD43 proteins in the tissues of 25 MALToma (case group) and 25 benign lymphadenosis (control group) was detected by immunohistochemistry. Results Abnormal CD21+follicular dendritic cells (FDC) meshes were found in all patients of case group. Most of the FDC meshes were sparse and broken, and a few were enlarged or fused into pieces. Intact CD21+FDC meshes were all found, and abnormal FDC meshes were not found in control group. The positive rate of abnormal FDC meshes in case group was significantly increased compared with that in control group (χ2 = 46.080, P= 0.000). The expression rate of CD43+in CD20+cells was 24 % (6/25) in case group, but it was negative in control group (χ2=4.375, P=0.030). Conclusions Abnormal CD21+FDC meshes and CD43+expression in CD20+cells are useful in the differential diagnosis between MALToma and benign lymphadenosis. The abnormal FDC meshes of MALToma are enlarged or fused in the minority of cases.

Using the high efficient copper-adsorbing yeast strain Y17 as absorbing material, the major affect factors including pH, original concentration of Cu2+, cell biomass, adsorption time and temperature were examined, and then the absorbing sites of the Y17 was determined. The results showed that the solution pH was the most dominate factor which affected the biosorption of Cu2+, the other affecting factors were the ini- tial concentration of Cu2+, the cell biomass added, and adsorption time, respectively; the temperature had lit- tle effect on the rate of biosorption. The orthogonal experiment showed that the optimal absorption condition was as follow: the solution pH was 5.0, the absorption time was 40 min, the cell biomass of Y17 added was 5.0 g/L, and the concentration of Cu2+ was 8 mmol/L; the highest adsorbing rate was up to 82.7% at this condition. Based on the results of different pretreatments and the desorption of Cu2+, the cell wall of Y17 was identified as the main place occurring boisorption process, and the -NH2 group, -COOH group on the surface of the yeast cells played an important role on the boisorption process.

Objective:To study the social psychology of people when facing severe acute respiratory syndrome(SARS),a sudden social crisis.Methods:Using the social psychological methods,we analyzed the manifestations,causes and hazards of the public fear derived from the outbreak of SARS in China.Results:Sudden outbreak of SARS made people full of uncertainty and fear,and such fear would bring adverse effects to the society.It affected not only the daily life of people,but aslo their physical, mental stability and behavior reaction.Conclusion:SARS makes us realize the importance of psychology and psychiatry to individuals and to the society.It is imperative to establish a comprehensive provention and intervention system of diseases to maintain the social stability and the well being of individuals.