1.Analytical method for benzene, toluene, xylene, hexane, trichloroethylene in gooey agent by gas chromatography.
Hui-Fang YAN ; Xiao-Shuang XIE ; Hui-Fang DU ; Jing ZHANG ; Chun-Ling LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(4):303-305
Benzene
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analysis
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Chemical Industry
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Chromatography, Gas
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methods
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Humans
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Occupational Exposure
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analysis
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Toluene
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analysis
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Trichloroethylene
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analysis
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Xylenes
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analysis
2.Recurrent malignant epithelioid angiomyolipoma of kidney: report of a case.
Jiang DU ; Yu-lan JIN ; Chun-yan HE ; Ming LI ; Hong-gang LIU
Chinese Journal of Pathology 2010;39(4):275-276
Adult
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Angiomyolipoma
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metabolism
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pathology
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surgery
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Carcinoma, Renal Cell
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metabolism
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pathology
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Diagnosis, Differential
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Humans
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Kidney Neoplasms
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metabolism
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pathology
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surgery
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MART-1 Antigen
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metabolism
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Male
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Melanoma-Specific Antigens
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metabolism
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Tumor Suppressor Protein p53
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metabolism
4.Monitoring density of mosquitoes and resistance of major vector mosquitoes to insecticides in Zigong, Sichuan
LI Chun-yan ; TIAN Feng-yuan ; LING Yuan-nong ; DU Yu
China Tropical Medicine 2022;22(09):866-
Abstract: Objective To investigate the population densities composition and seasonal change of mosquitoes, and resistance of Culex pipiens quinquefasciatus and Aedes albopictus several commonly used insecticides in Zigong, so as to provide a theoretical basis for guiding the scientific rational selection and use of chemical insecticides, as well as the prevention and control of mosquito-borne diseases. Methods The surveillance data on mosquitoes by traps were collected from 2018 to 2020 in Zigong. The wild Culex pipiens quinquefasciatus and Aedes albopictus were collected throughout the city and reared to F1 generation in lab. The resistance of adult Culex pipiens quinquefasciatus and Aedes albopictus to these commonly insecticides was detected by contact tube method recommended. The statistical analysis on the monitoring results was used by Excel 2010. Results The annual average mosquito densities of monitoring sites were 1.11, 10.71, and 4.81 mosquitoes/(lamp·h), respectively from 2018 to 2020. The seasonal density of mosquitoes showed a single peak curve distribution throughout the year from 2018 to 2020 in Zigong, with the highest in July. There were obvious differences in mosquito density between different habitats, with the highest density of mosquitoes in barn. The 24 h mortality of Aedes albopictus and Culex pipiens quinquefasciatus to beta-cypermethrin, lambda-cyhalothrin, deltamethrin, permethrin, malathion, fenitrothion, chlorpyrifos, propoxur, and bendiocarb were 70.71% and 3.85%, 26.53% and 1.68%, 19.79% and 1.94%, 40.80% and 0.81%, 97.98% and 3.85%, 100.00% and 7.52%, 100.00% and 66.34%, 96.15% and 33.60%, 100.00% and 71.43%, respectively. The adult Aedes albopictus larvae in Zigong was sensitive to fenitrothion, chlorpyrifos, bendiocarb, showed resistance to beta-cypermethrin, lambda-cyhalothrin, deltamethrin, permethrin, and showed resistance dubiously to malathion and propoxur. The adult Culex pipiens quinquefasciatus larvae in Zigong have developed certain resistance to these commonly insecticides. Conclusion Both Culex pipiens quinquefasciatus and Aedes albopictus had high resistance to pyrethroid insecticides, which suggested that we should strengthen the patriotic hygiene campaigns, reduce pyrethroid insecticides, use alternately different types of insecticides for delay the development of resistance and better mosquito prevention and control.
5.Meta-analysis on Reinhartdt and Sea Capsule combined with Donepezil in treatment of Alzheimer's disease
Biao DU ; dong Xiao FAN ; yan Chun ZHANG ; qiang Wen KONG ; Yang DU ; yang Chun ZHOU
Drug Evaluation Research 2017;40(7):987-992
Objective Objective To evaluate the efficacy and safety of Reinhartdt and Sea Capsule (RSC) combined with donepezil (experimental group) compared with donepezil (control group) in treatment of Alzheimer's disease.Methods The randomized controlled trials (RCT) of reinhartdt and sea capsule combine with donepezil in treatment of Alzheimer's disease were searched from Pubmed,VIP,CNKI,CBM,and Wangfang detabase by computer.Deadline from January 2000 to February 2017.References of included studies were also retrieved,extracted data,and assessed the methodological quality.Then,Meta-analysis was performed using RevMan 5.0 software.Results A total of eight RCTs were included,including 605 patients with insomnia.Meta-analysis results showed that compared with control group,experimental group MMSE score [P<0.001,MD=2.69,95%CI(1.46,3.92)],ADAS-Cog score [P<0.001,MD=-4.54,95%CI(-5.64,-3.43)] and ADL score [P<0.001,MD=-3.60,95%CI(-4.53,-2.66)],the difference was statistical;There was no signficant difference between two group in the incidences of adverse effect [P=-0.94,OR=1.02,95%CI(0.63,1.66)].Conclusion RSC combined with donepezil showed better efficacy for Alzheimer's disease,yet without increasing adverse effect rate as compared with donepezil alone.
6.Effects of polydatin on learning and memory and Cdk5 kinase activity in the hippocampus of rats with chronic alcoholism.
Xin-juan LI ; Yan ZHANG ; Chun-yang XU ; Shuang LI ; Ai-lin DU ; Li-bin ZHANG ; Rui-ling ZHANG
Chinese Journal of Applied Physiology 2015;31(2):117-120
OBJECTIVETo observe the effects of polydatin on learning and memory and cyclin-dependent kinase 5 (Cdk5) kinase activity in the hippocampus of rats with chronic alcoholism.
METHODSForty rats were randomly divided into 4 groups: control group, chronic alcoholism group, low and high polydatin group. The rat chronic alcoholism model was established by ethanol 3.0 g/(kg · d) (intragastric administration). The abstinence scoring was used to evaluate the rats withdrawal symptoms; cognitive function was measured by Morris water maze experiment; Cdk5 protein expression in the hippocampus was detected by immunofluorescence; Cdk5 kinase activity in the hippocampus was detected by liquid scintillation counting method.
RESULTSThe abstinence score, escape latency, Cdk5 kinase activity in chronic alcoholism group rats were significantly higher than those of control group (P < 0.05). The abstinence score, escape latency in high polydatin group rats were significantly lower than those of chronic alcoholism group (P < 0.05); Cdk5 kinase activity in high and low polydatin group rats was significantly lower than that of chronic alcoholism group( P < 0.05); immunofluorescence showed that the Cdk5 positive cells of chronic alcoholism group were significantly increased compared with control group (P < 0.05), and the Cdk5 positive cells of polydatin groups were significantly decreased compared with chronic alcoholism group ( P < 0.05).
CONCLUSIONPolydatin-reduced the chronic alcoholism damage may interrelate with regulation of Cdk5 kinase activity.
Alcoholism ; physiopathology ; Animals ; Cyclin-Dependent Kinase 5 ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; Glucosides ; pharmacology ; Hippocampus ; drug effects ; enzymology ; Learning ; drug effects ; Memory ; drug effects ; Rats ; Stilbenes ; pharmacology
7.Anti-tumor activity and molecular mechanism of C3C12PPD in lung cancer
Lu-Lu HUANG ; Chun-Xia LIU ; Qian-Qian DU ; Jin-Ling YANG ; Yan LI
Chinese Journal of Pharmacology and Toxicology 2018;32(4):286-287
OBJECTIVE The aim of the present study was to investigate the anti-tumor effect and mechanism of a novel compound, C3C12PPD, a bioactive unnatural ginsenoside by metabolically engi-neered yeasts based on a new UDP-glycosyl- transferase from Bacillus subtilis. METHODS MTT assay was used to analyze the anti-proliferation activity of C3C12PPD in vitro. The effect of anti-tumor activity was observed by mouse Lewis xenograft model in vivo.The effects of C3C12PPD on suppressing the angio-genesis and invasion of A549 cells were investigated in vitro using Transwell and tube formation assays. RNAseq was used to find tagets of C3C12PPD. Western blotting was performed to investigate the expres-sion level of proteins in tumor tissues treated with C3C12PPD. RESULTS C3C12PPD could inhibit the growth of lung cancer in vitro and in viv o. At the dosage of 10.0 mg·kg-1, C3C12PPD inhibited tumor growth by 40.0% (P<0.05) in tumor weight in mouse Lewis xenograft. The inhibition of tube formation was 77.5%(P<0.01)and 80.3%(P<0.01)following treatment with 1×10-4and 2×10-4mol·L-1C3C12PPD for 5 h, whereas the proliferation of EA.hy926 cells was not influenced under the above concentrations. Under the concentrations of 1×10-4mol·L-1,C3C12PPD inhibited invasive ability of A549 cells(P<0.05).The results of RNAseq susgested that antitumor activity of C3C12PPD were associated with epithelial-mesenchymal transition (EMT) and angiogenesis. Moreover, the proteins related to EMT, Raf/MEK/ERK and AKT/mTOR signal pathways were effected by C3C12PPD analysed by western blotting. CONCLUSION These data suggested that C3C12PPD was able to supress lung cancer through inhibit EMT, invision and angiogenesis.
8.Cost-effectiveness analysis of breast cancer multi-gene panel sequencing
Chinese Journal of Health Policy 2017;10(11):59-66
Objective:To model and evaluate the cost-effectiveness of next-generation sequencing(NGS)panel for the screening of breast and ovarian cancer using the decision-making model amongst UK Ashkenazi Jews(AJ) women in the German community,determining whether a multi-gene panel would be cost-effective in patients referred to high-risk of breast cancer when compared with no testing program.Methods:Based on the population epidemiology and cost data in the United Kingdom,a decision-analytic model was developed to compare lifetime costs and benefits associated with NGS panel.The cost-effectiveness analysis was analyzed from a payer,s perspective across a lifetime horizon.Multiple source data were used for estimating cancer incidence,total costs,life-years,quality-adjusted life-years(QALYs)and incremental cost-effectiveness ratio(ICER).Costs were reported at 2014 prices and discounted at 3.5%.Deterministic and probabilistic sensitivity analysis(PSA)based on Monte Carlo Simulations were per-formed to evaluate the robustness of model.Results:In the base-case analysis,compared with no screening strategy, multi-gene panel test lowered the cancer incidence by 1.1%(0.75% for breast cancer,0.47% for ovarian canc-er),and gained an additional 0.87 life years and 0.89 QALYs,resulting in a discounted ICER of? 6766/QALY. Considering 70%testing uptake,this led to 601 fewer breast cancer cases and 283 fewer ovarian cancer cases.One-way sensitivity analysis indicated that the model was robust to variations for most of model parameters.The penetrance rate of breast cancer susceptibility genes was an important factor affecting overall results of the model.PSA showed that the probability for NGS panel being cost-effective at the threshold of? 20000/QALY-£ 30000/QALY was over than 90%,compared with no screening strategy.Conclusion: NGS panel testing of breast cancer was cost-effective compared with no screening strategy,especially for those AJ women with high mutation rates.
9.FK228 blocks cells survival signal pathways and induces apoptosis of prostate cancer DU145 cells
Ming ZHAO ; Sheng-Yu WANG ; Chun-Mei HOU ; Zhi-Yan DU ; Yuan-Ji XU ; Xiao-Dan YU ;
Chinese Journal of Cancer Biotherapy 1995;0(02):-
Objective:To investigate the underlying mechanism of histone deacetylase (HDAC) inhibitor FK228-in- duced apoptosis of the prostate cancer cell line DU145.Methods:The inhibitory effect of FK228 on DU145 cell growth and its cytotoxicity were determined by MTT assay;cell cycle arrest was detected by flow cytometry assay;morphological change was observed by Giemsa staining;and defined kinase protein levels were determined by Western blot analysis.Re- suits:FK228 obviously inhibited DU145 cells growth,arrested cell cycle at G_0/G_1 phase,induced cells morphological changes and degraded several kinase proteins,including EGFR,Her2,Raf-1,Src,Cdk4 and IAP member Survivin.The degradation of these kinases blocked Raf-Mek-Erk and PI3K/Akt survival signal pathways,inducing apoptosis.Condu- sion:FK228 may induce DU145 cell apoptosis through depletion of multiple kinase proteins and blockade of survival sig- nal pathways of DU145 cells.
10.Effects of electroacupuncture on the expression of GDNF and Ret in Parkinson's disease model rats.
Yan-Chun WANG ; Yu-He CHENG ; Jun MA ; Shui-Yong GAN ; Shu-Ju WANG ; Hua ZHOU ; Yan-Jun DU ; Min YANG ; Feng SHEN
Chinese Acupuncture & Moxibustion 2010;30(9):739-743
OBJECTIVETo explore the mechanism of electroacupuncture therapy on Parkinson's disease (PD).
METHODSFifty Wistar rats were randomly divided into a normal group, a sham-operation group, a model group, a Fengfu-Taichong group and a Shuanggu Yitong group. PD model was duplicated by microinjection of 6-Hydroxyl-Dopamine into right corpora striata, and by microinjection of normal saline in sham-operation group. Rats in normal group, sham-operation group and model group were not treated. In Fengfu-Taichong group, the rats were treated by electroacupuncture at "Fengfu" (GV 16) and "Taichong" (LR 3) on the basis of the PD model, and by electroacupuncture at "Fengfu" (GV 16), "Taichong" (LR 3), "Guanyuan" (CV 4) and "Zusanli" (ST 36) in Shuanggu Yitong group, once daily for 2 weeks. GDNF and Ret expression were detected by immunohistochemistry and western blotting, respectively.
RESULTSThe number of GDNF positive cells and the content of Ret receptor increased significantly in the two electroacupuncture groups compared with those in the other groups (all P < 0.01), and the expression of GDNF increased significantly in Shuanggu Yitong group compared with that in Fengfu-Taichong group (P < 0.01).
CONCLUSIONElectroacupuncture can not only increase the expression of GDNF, but also enhance its effect. "Shuanggu Yitong" method is better than simple acupuncture at "Fengfu" (GV 16) and "Taichong" (LR 3) in increasing expression of GDNF.
Animals ; DNA-Binding Proteins ; genetics ; metabolism ; Disease Models, Animal ; Electroacupuncture ; Gene Expression ; Glial Cell Line-Derived Neurotrophic Factor ; genetics ; metabolism ; Humans ; Nuclear Proteins ; genetics ; metabolism ; Parkinson Disease ; genetics ; metabolism ; therapy ; Random Allocation ; Rats ; Rats, Wistar