Venous thrombotic disease is a serious disease,which impact on health and life-threatening.Pulmonary embolism and deep-vein thrombosis are the two components of a single disease called venous thromboembolism in obstetrics and gynecology.but it can be difficult to diagnose because clinical symptoms and signs are non-specific or absent in early venous thrombus embolism (VTE).It has great value that how to use the most economic,simple,efficient method for screened high-risk groups,timely and accurate laboratory diagnosis of VTE.

Objective:To study the surface enhanced Raman scattering ( SERS) of safflower and identify safflower injections by SERS quickly and effectively. Methods:Through comparative analysis of the Raman spectroscopy of safflower injections and the corre-sponding control herbs, the rapid identification of safflower injections was realized. Results:The results showed that several character-istic peaks of safflower were enhanced obviously in SERS, which could be used to identify safflower injections. Conclusion:The meth-od is reliable, rapid, accurate and specific, which can be applied as a method to identify safflower and its injections.

ObjectiveTo develop the military acute stress scale and examine the reliability and validity of this scale.MethodsBased upon the previous acute stress researches and the rule of psychometrics,the military acute stress scale was compiled according to open-ended inventory survey.A total of 630 personnel were chosen by random cluster sampling,and tested with this scale,50 of them were tested by Chinese Military psychosomatic health Scale simultaneously,and 50 of them were retested after two week.The reliability and validity were examined by exploratory factor analysis,confirmatory factor analysis and correlation analysis.ResultsThe military acute stress scale was comprised of 9 factors and one lie sub-scale ; Main fit indexes of model were the following CMIN =1219.476,DF =593,P =0.000,CMIN/DF =2.056,IFI =0.927,TLI =0.902,CFI =0.924,RMSEA =0.059; The conrelation coefficients between subscales and subscales were 0.284-0.572.The correlation coefficients between subscales and total scale were 0.700 ～ 0.846 (P ＜ 0.05 or 0.01 ) ; The correlation coefficients was significant between this scale and Chinese military psychosomatic health scale.The correlation coefficients were 0.338-0.962 (P ＜ 0.01 ).The test-retest correlation coefficients of total scale and subscales were 0.379-0.909 (P ＜ 0.01 ).The Cronbaeh's α coefficients were 0.606-0.926 ; and the split-half correlation coefficients were 0.466-0.855.ConclusionThe military acute stress scale has acceptable psychometrics properties on reliability and validity.

Fifty-six middle and aged post-herpetic neuralgia patients were treated with Jiaji (Ex- B 2) of the correspondingly affected segments plus Ashi points. The major points were treated with even reinforcing-reducing manipulation and the auxiliary points were treated by puncturing subcutaneously around the skin lesion with reducing manipulation. Afterward the three-edged needle was used to insert into the tenderness spots rapidly and remove immediately for 3-5 consecutive times around the spot plus flash-fire cupping. The result showed recovery in 36 cases; marked effect in 17 cases; no effect in 3 cases and the total effective rate was 94.6%.

Necrotizing enterocolitis(NEC) is a common and devastating gastrointestinal disease that occurs predominantly in premature infants.Despite various advances in management,the mortality of this disease remains high.During the last decade,studies have shown that heparin-binding epidermal growth factor-like growth factor (HB-EGF),can protect intestinal epithelial cells from various forms of injury in vitro.Furthermore,studies using both an intestinal ischemia-reperfusion injury model in adult rats and neonatal rat model of NEC,have shown that HB-EGF can protect the intestines from injury.Present studies provide a new research direction in the prevention and treatment.

Objective:To investigate the expression of five drug resistance genes in hepato cellular cancer,lung cancer,stomach cancer,colorectal cancer,ovarian cancer,breast cancer tissue. Methods:The expression of P-gp,MRP,LRP,GST-?,Topo-Ⅱ in 120 cases of malignant tumors were examined by immunohistochemistry stain. Results: 1. The expression of P-gp,MRP,LRP,GST-? in the 6 common cancers were relatively high,while Topo-Ⅱ was relatively low. 2. P-gp,MRP,LRP,GST-?,Topo-Ⅱ were all high expressed in hepatocellular cancer. 3. The coexpression rate of two and more genes was 95.83%,the coexpression rate of five genes was 21.67%. Conclusion:P-gp,MRP,LRP,GST-?,Topo-Ⅱ are associated with multidrug resistance in different degree,which can be regarded as valuable indicators for the decision of individual chemotherapeutic program.

Objective:To identify the best method for isolating and culturing mature rat adipocytes in vitro.Methods:Three different primary culture methods,i.e.organ culture,ceiling culture,and modified ceiling culture were used in the present study.Mature Sprague-Dawley rat adipocytes were cultured in vitro.The cells were observed under microscope and oil red O staining to compare the distinction of cells with different culture methods in the same condition.Rusults:Adipocytes were successfully cultured by the three primary cell culture methods.Cells cultured were highly homogenous.Modified ceiling culture method was the best in terms of the efficiency of culturing and cell morphology.Conclusion:Modified ceiling culture,is a long-term stable primary culture method for mature rat adipocyte,providing a good technique platform for the studies related to adipocytes.

Objective:To explore the opportunity and the manner of termination of pregnancy.Methods:The data of 200 cases of ICPfrom January 2007 to June 2008 were retrospectively analyzed.Maternal pruritis,liver function ,glycocholic acid(CG),amniotic fluid condition, the time and the way of termination of pregnancy,Apgar score were examined.Three groups were classified by the values of CG:Group A (CG

Objective:To observe the effects of radix astragali on hypertrophic scars in rabbit ears. Methods:Twenty healthy adult Japanese big ear rabbits were given hypertrophic scars over the ventral surface of the ears and on the 21st day after wound,animals were randomly divided into the treated groups (groups A,B,and C were treated with radix astragali in different concentrations)and the control groups(groups D and E were treated with saline and blank control,respectively). On the 32nd and 43rd day after wound,the thickness and hardness of scar were measured.The TGF-?1mRNA were detected in hypertrophic scar samples with RT- PCR.The change of scar tissues was observed with H&E staining and immunohistochemical staining with TGF-?1. Results:The thickness and hardness of scar were inversely correlated with the concentration of radix astragali in the radix astragali-treated group(P0.05)between the 32nd day and43rd day groups.Conclusion:Radix astragali could inhibit proliferation of hypertrophic scars in the rabbit ears by decreasing the expression of TGF-?1mRNA and its protein. Thereby radix astragali may have beneficial effects in the treatment of hypertrophic scar.

0.05).Conclusion:The results support our hypothesis that APS can be used to treat the hematopoietic type of acute radiation sickness.APS pretreated groups had better results than APS treated groups in initial observation.