Objective To investigate the feasibility of application of plasma anticoagulant heparin and hepa-rin lithium instead of serum in clinical biochemistry.Methods The clinical data 50 healthy persons in our hospital were statistically analyzed.Results The Potassium (K),sodium (Na),calcium (Ca),low -density lipoprotein (LDL),lactate dehydrogenase(LDH),cytokines(CK),blood urea nitrogen(BUN),serum uric acid(UA),alkaline phosphatase(ALP),magnesium(Mg),the standard value of total cholesterol(CHO),serum total bilirubin(TBil), direct bilirubin(DBil),triglyceride(TG)and other items of anticoagulant heparin plasma were (4.42 ±0.27)mmol/L, (137.00 ±1.70)mmol/L,(2.56 ±0.41)mmol/L,(115.53 ±88.93)mmol/L,(5.06 ±1.18)mmol/L,(281.56 ± 67.36)mmol/L,(84.41 ±74.02)μmol/L,(0.99 ±0.18)U /L,(4.62 ±1.26)μg/L,(17.18 ±15.66)mmol/L, (2.22 ±2.66)μmol/L,(2.28 ±7.70)mmol/L,which were significantly lower than those in serum(t =-3.415,-2.017,-4.739,-3.571,-3.155,-2.778,-4.117,-7.65,-10.799,-5.677,-3.192,-8.625,-5.401,-3.483,all P <0.05.);The K,Na,TCa,BUN,UA,ALP,DBil,TP,Mg,CK,HDL -C,LDL,LDH and other items of lithium heparin anticoagulant plasma were (4.51 ±0.30)mmol/L,(137.06 ±1.56)mmol/L,(2.60 ±0.08)mmol/L, (5.08 ±1.22)mmol/L,(281.86 ±67.78)μmol/L,(84.83 ±74.82)U /L,(2.22 ±2.56)μmol/L,(74.15 ± 10.94)mmol/L,(0.99 ±0.17)μg/L,(118.47 ±92.77)U /L,(1.68 ±0.72)mmol/L,(2.55 ±0.66)mmol/L, (129.82 ±30.34)U /L,which were significantly lower than those in serum(t =-3.517,-3.273,-4.128,-3.101,4.749,-2.271,-4.586,-6.706,-10.095,-5.837,2.234,-7.309,-7.175,-14.875,all P <0.05.);The HDL -C,LDL,BUN,Cr,TBil,DBil,Na,Cl,UA,GGT,ALP,ALT,AST,TP,ALB,TBA,Mg,CHO,LDH, CK,CK -MB,AMS,TG,Glu 24 and other items of heparin lithium heparin anticoagulant plasma and serum had good correlation(r >0.800).Conclusion Plasma anticoagulant heparin and heparin lithium instead of serum is feasible in clinical biochemistry.

Objective To investigate the relationship between hOGG1 and XPD gene polymorphism and genetic susceptibility of gastric cancer.Methods This study was carried out in Dongying Shengli Petroleum Administration Bureau Hospital.Under the narrow band imaging(NBI) mode,the blood samples of a total of 98 gastric cancers and 80 controls without cancers were collected.Genetic polymorphisms of DNA repair genes were identified by polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP),then its relationship with cancers was analyzed.Results Carrying 326Cys allele with the wine increased the risk of gastric cancer; Lys 751Gln genotype increased the susceptibility of the gastric cancers (OR =1.486,0.73 ～ 3.025).Conclusion Lys751 Gln genotype increases the risk of gastric cancer.

Objective To observe the efficacy of Foley balloon combining with uterine adhesions to prevent the recurrence of intrauterine adhesions after resection of intrauterine adhesions.Methods Patients underwent resection of intrauterine adhesions were randomized into two groups. 30 patients in control group underwent the therapy of uterine adhesions to prevent the relapse intrauterine. On the other hand, the study group with 30 patients were placed Foley balloon for 5 ~ 7 days and combined with the therapy of uterine adhesions to intervention for 1 ~ 2 weeks. All patients were followed-up for 1 ~ 2 months, and the result of the intrauterine adhesion were assessed by hysteroscopy.Results The rate of the relapse in control group was 3.33%; the study group had the same result (the rate was 3.33%). There was no difference between these two groups.Conclusions The rate of recurrent intrauterine adhesions is no different between the control group of only use therapy of uterine adhesions and replacement of Foley balloon combining with therapy of uterine adhesions as intervention.

Objective To explore the effects of yttrium nitrate on hemogram in rats Methods 48 rats were randomly divided into 4 groups,such as one control group treated with distilled water and 3 exposure groups treated with 70 mg/kg,14 mg/kg and 2 8 mg/kg yttrium nitrate via peritoneal injection twice a week for 6 weeks.After the continous 6 week exposure,the differences of hemogram between control group and exposure groups were observed Results No significant differences were observed in the counts of red blood cells,white blood cells and platelets,the contents of hemoglobulin and coagulation times between control group and each exposure group( P

AIM To study the protective effect of TFA against cerebral ischemia reperfusion injury. METHORDS The cerebral ischemia model in mice was made by means of ligating bilalateral common carotid arteries.The mice survive rate during 6 h was observed,and malondialdehyde (MDA) in the ischemic cerebral cortex was measured. Using nitrogen anoxia model in mice,the survive time was observed. Ligating bilateral common carotid arteries and descending blood pressure,the cerebral ischemia reperfusion model in rabbits was established. The brain of rabbits was initiated by ischemia for 60 min followed by 30 min of reperfusion. The electroencephalogra phy (EEG) of cerebral ischemia and reperfusion was recorded. Malondialdehyde(MDA) and lactate dehydrogenase(LDH) in the ischemic cerebral cortex were measured. RESULTS TFA(30,60,120 mg?kg -1 ) prolonged the survive time after anoxia in mice,enchanced the survive rate after cerebral ischemia and inhibited the increasing of MDA contents in the cerebral cortex in mice.TFA(12,24,48 mg?kg -1 ) inhibitid the changes of EEG,MDA and LDH induced by cerebral ischemia reperfusion in rabbits. CONCLUSION TFA has protective effects on cerebral ischemia reperfusion injury,the mechanism may relate to attenuating free radical and lipid peroxidation.

Object To study the chemical constituents from leaves of Diplopanax stachyathus Hand Mazz aiming at searching for bioactive natural products Methods The compounds Ⅰ-Ⅹ were isolated by a combination of Sephedex LH 20 and silica gel column chromatographies, and their structures were identified by spectral methods Results Ten known compounds were isolated from leaves of D stachyathus Their structures were identified by means of spectroscopic analysis as: ? sitosterol (Ⅰ), betulinic acid (Ⅱ), oleanolic acid (Ⅲ), ursolic acid (Ⅳ), daucossterol (Ⅴ), quercetin 3 O ? D glucopyranoside (Ⅵ), 3,3′,4′ tri O methyellagic acid (Ⅶ), 3, 3′ di O methylellagic acid 4 O ? D xylopyranoside (Ⅷ), 3′ O methylellagic acid 4 O ? D xylopyranoside (Ⅸ), and 3, 3′,4′ tri O methylellagic acid O ? D glucopyranoside (Ⅹ) Conclusion Compounds Ⅰ-Ⅵ and Ⅸ are isolated from this plant for the first time

Objective To investigate the mechanism of mizolastine inhibiting allergic contact dermatitis (ACD) in murine model. Methods The murine model of allergic contact dermatitis by topical DNFB was used. Using this model murine inhibition of ear swelling was observed after oral administration of mizolastine in different doses. The levels of IFN-?, TNF-? and IL-4 in the sera of these mice were detected by enzyme-linked immunosorbent assay (ELISA). Results Murine ear swellings were markedly suppressed in each dose group of mizolastine (P 0.05). Different doses of mizolastine inhibited the expression of these three cytokines to different degrees. Conclusion The therapeutic effect of mizolastine on murine ACD may be played by inhibiting the expression of some cytokines.

Objective To evaluate method of CT simulation (CT sim) in the treatment planning setup of esophageal carcinoma. Methods From Dec.1999 to Jun.2002, 49 patients with pathologically proved esophageal carcinoma were analyzed. All patients underwent CT sim and treatment planning was configured with three symmetrical fields in the isocenter mode. A few comparisons have been made between CT sim and conventional simulation (Con sim) in the treatment planning setup. Results The difference in isocenter position were observed between CT sim and Con sim with 3～18 mm.When the esophageal lumen was taken as portal center, the 90% isodose curve in 42.8%(21/49) of patients were able to cover the whole lesion. When taking the tumor as the portal center, the whole lesion of all patients was totally covered by the 90% isodose curve. Conclusion The CT sim has an advantage in the delineation of lateral extension of esophageal tumor than the Con sim .Selection of irradiation portal should be done according to the size the silhouette of the tumor.

Aggressive T cell non-Hodgkin lymphomas are a group of highly malignant tumors characterized by rapid progress and short survival.This is especially true for patients who have a poor response to treatment because of their drug resistance.Hence,to clarify the mechanism of this resistance will have important guiding significance in reversing drug resistance and improving poor prognosis of aggressive T cell nonHodgkin's lymphomas.