Objective To investigate the relationship between hOGG1 and XPD gene polymorphism and genetic susceptibility of gastric cancer.Methods This study was carried out in Dongying Shengli Petroleum Administration Bureau Hospital.Under the narrow band imaging(NBI) mode,the blood samples of a total of 98 gastric cancers and 80 controls without cancers were collected.Genetic polymorphisms of DNA repair genes were identified by polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP),then its relationship with cancers was analyzed.Results Carrying 326Cys allele with the wine increased the risk of gastric cancer; Lys 751Gln genotype increased the susceptibility of the gastric cancers (OR =1.486,0.73 ～ 3.025).Conclusion Lys751 Gln genotype increases the risk of gastric cancer.

Objective To investigate the feasibility of application of plasma anticoagulant heparin and hepa-rin lithium instead of serum in clinical biochemistry.Methods The clinical data 50 healthy persons in our hospital were statistically analyzed.Results The Potassium (K),sodium (Na),calcium (Ca),low -density lipoprotein (LDL),lactate dehydrogenase(LDH),cytokines(CK),blood urea nitrogen(BUN),serum uric acid(UA),alkaline phosphatase(ALP),magnesium(Mg),the standard value of total cholesterol(CHO),serum total bilirubin(TBil), direct bilirubin(DBil),triglyceride(TG)and other items of anticoagulant heparin plasma were (4.42 ±0.27)mmol/L, (137.00 ±1.70)mmol/L,(2.56 ±0.41)mmol/L,(115.53 ±88.93)mmol/L,(5.06 ±1.18)mmol/L,(281.56 ± 67.36)mmol/L,(84.41 ±74.02)μmol/L,(0.99 ±0.18)U /L,(4.62 ±1.26)μg/L,(17.18 ±15.66)mmol/L, (2.22 ±2.66)μmol/L,(2.28 ±7.70)mmol/L,which were significantly lower than those in serum(t =-3.415,-2.017,-4.739,-3.571,-3.155,-2.778,-4.117,-7.65,-10.799,-5.677,-3.192,-8.625,-5.401,-3.483,all P <0.05.);The K,Na,TCa,BUN,UA,ALP,DBil,TP,Mg,CK,HDL -C,LDL,LDH and other items of lithium heparin anticoagulant plasma were (4.51 ±0.30)mmol/L,(137.06 ±1.56)mmol/L,(2.60 ±0.08)mmol/L, (5.08 ±1.22)mmol/L,(281.86 ±67.78)μmol/L,(84.83 ±74.82)U /L,(2.22 ±2.56)μmol/L,(74.15 ± 10.94)mmol/L,(0.99 ±0.17)μg/L,(118.47 ±92.77)U /L,(1.68 ±0.72)mmol/L,(2.55 ±0.66)mmol/L, (129.82 ±30.34)U /L,which were significantly lower than those in serum(t =-3.517,-3.273,-4.128,-3.101,4.749,-2.271,-4.586,-6.706,-10.095,-5.837,2.234,-7.309,-7.175,-14.875,all P <0.05.);The HDL -C,LDL,BUN,Cr,TBil,DBil,Na,Cl,UA,GGT,ALP,ALT,AST,TP,ALB,TBA,Mg,CHO,LDH, CK,CK -MB,AMS,TG,Glu 24 and other items of heparin lithium heparin anticoagulant plasma and serum had good correlation(r >0.800).Conclusion Plasma anticoagulant heparin and heparin lithium instead of serum is feasible in clinical biochemistry.

Objective To investigate the effect of coenzyme Q10 combined with Shenmai injection on serum enzyme in the treatment of brain hypoxic injury after asphyxia by meconium in newborn.Methods 64 cases with brain hypoxic injury after asphyxia by meconium from Medical University of Tianjin Jinghai Clinical College were selected and randomly divided into 2 groups, 32 cases in each group.The control group received maintained ventilation and circulation function and routine drug therapy adequate, and the experiment group received more with coenzyme Q10 combined with Shenmai injection for 7 days.Serum enzymes and myocardial injury markers, oxidative stress and inflammation related factors and the clinical effect and complications were compared after the treatment.Results Compared with before treatment, levels of CK-MB, AST, LDH, CK and α-HBDH decreased in two groups after the treatment, levels of CT-1, CTnI and Mb decreased, levels of SOD and MDA decreased, contents of GSH-Px, APN, IGF-1 increased, contents of Leptin decreased (P<0.05).Compared with the control group, the levels of CK-MB, AST, LDH, CK and α-HBDH in the experiment group were lower, levels of CT-1, CTnI and Mb were lower, levels of SOD and MDA were lower, contents of GSH-Px, APN, IGF-1 were higher, contents of Leptin were lower(P<0.05).The clinical curative effect rate of control group(65.63%) was lower than the experiment group (87.50%)(P<0.05).Conclusion Coenzyme Q10 combined with Shenmai injection in the treatment of brain hypoxic injury after asphyxia by meconium in newborn is curative effective with high safety, and it can reduce serum enzyme and myocardial injury.

Objective To evaluate method of CT simulation (CT sim) in the treatment planning setup of esophageal carcinoma. Methods From Dec.1999 to Jun.2002, 49 patients with pathologically proved esophageal carcinoma were analyzed. All patients underwent CT sim and treatment planning was configured with three symmetrical fields in the isocenter mode. A few comparisons have been made between CT sim and conventional simulation (Con sim) in the treatment planning setup. Results The difference in isocenter position were observed between CT sim and Con sim with 3～18 mm.When the esophageal lumen was taken as portal center, the 90% isodose curve in 42.8%(21/49) of patients were able to cover the whole lesion. When taking the tumor as the portal center, the whole lesion of all patients was totally covered by the 90% isodose curve. Conclusion The CT sim has an advantage in the delineation of lateral extension of esophageal tumor than the Con sim .Selection of irradiation portal should be done according to the size the silhouette of the tumor.

OBJECTIVE:To detect and analyze the drug resistance of the AmpC and the extended-spectrum ?-lactamases(ESBLs)in Escherichia coli(E coli)from urinary infections.METHODS:ESBLs was detected by Phenotypic Confirmatory Test and AmpC was detected by improved three dimensional test.RESULTS:The isolating rates of ESBLs(45 strains),AmpC(8 strains)and AmpC+ESBLs in E.coli(5 strains)were 34.6%,6.2% and 3.8%,respectively.The drug resistance rates of ESBLs and AmpC-producing E.coli was higher than those of the strains without producing ESBLs or AmpC.CONCLUSION:Production of AmpC and ESBLs in ?-lactam antibiotics was the main resistance mechanism against E.coli.Carbopenems should be regarded as the first choice in clinical empirical medication for enzyme-producing strains.

Objective To discuss the importance of early di agnosis and treatment of primary hy-perparathyroidism in multiple bone resorptions.Methods From April 1997to April 2001,5patie nts were regarded as having multiple bone resorptions.There were 4females and 1male.The age ranged from 25to 46years(average 37years).The mean interval between symptom o nset and final diagnosis was 19.5mon ths(from8to 36months).All patients were suffering from se vere bone pain in need of strong antalgic,and the symptoms including fatigue,depression,loss of appetite and malaise,e tc.Abnormality on bone scan indicated multiple areas of skeletal involvem ent,bone radiographs showed multip le bone resorptions in skull,rib,pelvis and limbs.The range of serum calcium level was 2.27-3.22mmol /L and that o f PTH was 168-214ng /ml.Palpable neck mass was found i n three patients.Results All patients had undergone resectio n of the abnormal parathyroid.Three were parathyroid adenomas and two defined a s hyperplastic glands.All of them had pain relief after operation.The average follow-up was 15months(3-27months);the bone lesions have been repaired partially or totally s hown in X -ray examination during follow-up.Conclusion Clinically,in younger patients with multiple bone resorptions,hyperparathyroidism should be highly suspected,so that timely parathyroid surgery can be ca rried out for the treatment.[

BACKGROUND:There is no design that can completely rule out the intermittent impact damage to implants, therefore, a new bal attachment-retained implant system is constantly updated and developed. OBJECTIVE:To evaluate the clinical effectiveness of an implant-supported mandibular overdenture retained with a bal attachment. METHODS: We searched the Cochrane Library, PubMed, Medline, EM-base, WanFang Data, CNKI, VIP and other databases by computer to colect randomized controled trials addressing the implant-supported mandibular overdenture retained with a bal attachment and other control methods for dentures. The time limit was from database creation to February 2014. Two researchers independently completed literature screening according to inclusion and exclusion criteria, data extraction and quality assessment. RevMan 5.1 software was used for meta-analysis. RESULTS AND CONCLUSION: There were 10 studies included in result analysis, including 7 from China and 3 from other countries. Analysis results showed that statistical heterogeneity was remarkable in included studies, and there was no significant difference in patient’s satisfaction, clinical and objective indicators, and complications, suggesting that this approach continues to be explored in clinic. The implant-supported bal attachment-retained mandibular overdenture is relatively expensive, which is identical with the current research progress that is in the exploration stage worldwide. Due to the limited quantity and quality of included studies, the conclusions of this systematic review only provide references for clinical practice and research. The implant-supported bal attachment-retained mandibular overdenture stil needs further exploration and improvement.

BACKGROUND:Recent studies have shown that osteoclast differentiation factor is closely related to osteoclast differentiation, formation and function in bone remodeling during orthodontic tooth movement. OBJECTIVE:To observe the effects of three kinds of orthodontic appliances on the expression of osteoclast differentiation factor at the pressure side of rat periodontal tissue during remodeling process and to explore the biocompatibility of the orthodontic appliances with the host tissues during orthodontic treatment. METHODS:Eighty healthy Wistar rats were selected to establish animal models of orthodontic tooth movement, and then randomly divided into four groups: control group, MBT group, Begg group, Damon Ⅲ appliance group. Four animal from each group were sacrificed at 3, 7, 14 days after wearing orthodontic appliances. The tartrate-resistant acid phosphatase staining was used to count the osteoclasts at the pressure side of alveolar bone tissue; real-time quantitative PCR detection to detect mRNA expression of osteoclast differentiation factor at the pressure side of periodontal tissue and time distribution characteristics. RESULTS AND CONCLUSION:Compared with the control group, the number of positive osteoclasts and mRNA expression of osteoclast differentiation factor at the pressure side of the alveolar bone tissue were increased with orthodontic time, reached the peaked at 7 days and then gradualy decreased. The number of positive osteoclastsand mRNA expression of osteoclast differentiation factor at the pressure side of the alveolar bone tissue were significantly higher in the Damon Ⅲ group than the other three groups at 7 days after orthodontic treatment (P < 0.05). These findings indicate that, during the bone remodeling, the number of positive osteoclasts changed in accordance with the mRNA expression of osteoclast differentiation factor, and at 7 days, the number of positive osteoclasts and mRNA expression of osteoclast differentiation factor were highest in the Damon Ⅲ group.

Objective To observe the treatment effect of renal function and blood pressure in patient with blood pressure morning surge.Methods 34 patients take levoamlodipine 2.5 mg/q orally.35 patients take Fosinopril 10 mg/q orally.35 patients take Fosinopril lOmg/q and levoamlodipine 2.5 mg/q orally,continuing 12 months. Blood pressure,urinary albumin excretory rate,scrllm creatinine,urinary creatinine and calculated creatinine clearance at base line were measured.Results Blood pressure (P<0.01) and urinary albumin excretory rate (P<0.01) were decreased by compare to pretherapy in three groups.Post-treatment,urinary albumin excretory rate was reduced from 103.8±44.2 to (84.6±37.5)μg/min in the first group,from 102.9±45.4 to(82.7±38.4)μg/min in the second group,and from 109.8±40.5 to(51.2±40.1)μg/min in the last group.The difference for urinary albumin excretory rate was significant between prior treatment and post-treatment in every group (P<0.05).It is the same between the first group and the second.The difference for urinary albumin excretory rate was significant between the first group and the third group (P<0.01).The difference was significant between the second groud and the third group (P<0.01).Post-treatment,calculated creatinine clearance rate was increased from 84.6±7.4 to 88.2±7.1 in the first group,from 81.4±2.5 to 84.5±4.2 in the second group,and from 79.1±6.8 to 109.6±16.1 in the last group.The difference for calculated creatinine clearance rate was significant between prior treatment and post-treatment in the third group (P<0.01).Conclusion Blood pressure (P<0.01) and urinary albumin excretory rate were decreased by three methods.Three methods all protect renal function,and the third method is the best.

Radioactive 125I interstitial brachytherapy is easy to cause the intestinal tract tissue damage.Its mechanism possibly correlates to the quick renewal speed of the intestinal tract tissue and a majority of ceils in M, G2 and late S phases. The tolerant dose of intestinal tract tissue is approximately 160 Gy. If the absorbed dose is higher than the tolerant one, the intestinal tract tissue will be damaged. In the clinical practice of radio-active 125I interstitial brachytherapy, through the strict plan of the TPS system before the operation, by using ul-trasound, CT, MR/and other imaging technologies in the operating process and establishing the suitable barrier protection between the seeds and intestinal tract tissue, the radioactive damage of the intestinal tract tissue my be reduced.