Objective To observe temporal expression of matrix metalloproteinases(MMP-13) and tissue inhibitor of metalloproteina-ses-1 (TIMP-1) in lung of newborn rats with hyperoxia induced chronic lung disease (CLD),and to explore the relationship of CLD with MMPs.Methods The neonatal rats within 24 hours after birth were randomly divided into hyperoxia-exposed group(n=40) and control group(n=40).On postnatal 1,3,7,14 and 21 days,lung tissue of rats in 2 groups were collected.Lung histological changes were evaluated by hematoxylin and eosin and Masson stain;Collagen Ⅰ was detected by enzyme linked immunoadsorbent assay;MMP-13 and TIMP-1 were identifide by immunohistochemistry.Results Exposured to hyperoxia enviroment for 21 days,the number of alveolar decreased,terminal air space enlarged,inter-alveolar septa thickened,and deposition of interstitial collagen fibers.On 14 and 21 days,collagen Ⅰ in the lung of hyperoxia-exposed group increased significantly compared with that of control group(P0.05),obviously decreased on 21 day(P

Objective To study the effect of 2?-(3-hydroxypropoxy)-Calcitriol (ED-71) on the cell growth and differentiation of human osteoblast-like cell line OS-732. Methods Osteoblast-like cells were cultured with various concentration of ED-71.The effects of ED-71 on proliferation and differentiation were detected by (MTT), alkaline phosphatase activity and level of osteocalcin (bone gla protein,BGP). Results The proliferation rate of osteoblast-like cells cultured for 4 days was significantly inhibited by 10~ -7mol/L concentration of ED-71. ED-71 in 10~ -8, 10~ -9mol/L concentration can significantly increase BGP level, but ED-71 in 10~ -7mol/L concentration may decrease BGP level.Alkaline phosphatase activity compared with control was not changed significantly. Conclusions ED-71 can inhibit cell growth in high concentration and improve cell differentiation in lower concentration.

Background The orbital fibroblasts (OFs) in thyroid associated ophthalmopathy (TAO) play important roles in the proliferative and inflammatory response.Seeking the drug which inhibit OFs growth is of a vital significance for the prevention and treatment of TAO.Research documented that colchicine has an anti-fibrosis effect.But its influence on OFs of TAO patient is few known.Objective This study was to investigate the effect of colchicine on growth and apoptosis of OFs in vitro.Methods The retroobital connective tissue was obtained form 3 TAO patients and cultured using explant method.OFs were passaged and identified by immunochemistry,and 3-8 genetaions of cells were used in the study.Colchicine at the concentrations of 1 × 10-8,1 × 10-7,1 × 10-6,1 × 10-5,1 ×10-4 mol/L was added into the RPMI 1640 with 10％ fetal bovine serum(FBS) to incubated the cells for 24,48 and 72 hours respectively,and only RPMI 1640 was used to culture the cells as the control group.Cell counting kit-8 (CCK-8)was used to detect the absorbance value (A450) of OFs for the evaluatuion of OFs and the inhibitory rate of colchicine to OFs.The colchicine of 1 ×10-6,1 ×10-5,1 × 10-4 mol/L was added into the culture medium for 48 hours,and then the apoptotic rate of the cells and the cell percentage in various cellular cycle was assayed by flow cytometry(FCM).The expression of transforming growth factor-β (TGF-β)in the cells was detected by immunochemistry to assess the influence of colchicine on the serection of the cells.Results Cultured cells showed the spindle-like in shape and the cell number was significantly increased with the incubation time.After incubated with 1 × 10-4,1 × 10 5,1 × 10-6,1 ×10-7,1 × 10-8 mol/L colchicines,the A450 values were gradually reduced with the increase of the concentrations of colchicine(F ion =62.004,P＜0.05),and significant differences were found between different contrations of colchicine groups(all P＜0.05).Aslo,gradually declined A450 values of the cells were seen with the lapse of culture time among the groups(Ftime =459.582,P＜0.05).The inbitory rate of colchicine to the cells was elevated with the increase of concentrations.The apoptotic rates of the cells were (1.73 ± 0.15) ％,(21.04 ± 4.56) ％,(31.84 ±6.21)％and(35.32±5.56)％ in the control group and 1 × 10-6,1 × 10-5,1 × 10 4 mol/L colchicine groups respectively,with statistically significant difference among the 4 groups (F =83.905,P＜0.05).With the increase of concentrations of colchicines,the cell percentage in G2 +M phase lessened gradually,showing significant difference among the control group and the 1 × 10-6,1 × 10-5,1 × 10-4 mol/L colchicine groups (F =20.443,P＜0.05).The expression of the TGF-β in the cells was (97.60± 2.09) ％ in the control group,and that in the 1 × 10-4 mol/L colchicine group was (44.43 ± 3.96) ％,presenting a significant difference between them (t =65.330,P ＜ 0.05).Conclusions Colchicine can induce apoptosis of OFs and inhibit the prolilferation of OFs in a time-and dose-dependent manner probably by decreasing the TGF-β secretion

Animals ; Humans ; Interleukin-6 ; physiology ; Liver Cirrhosis ; physiopathology ; Mice ; Pulmonary Fibrosis ; physiopathology ; Rats

OBJECTIVETo observe the effect of Oxymatrine on left cardiac function and ventricular remodeling in rabbits after acute myocardial infarction.

METHODSLigation of the left anterior descending artery was adopted to establish acute myocardial infarction model, forty eight rabbits were randomized into the sham operation group, model group and Oxymatrine group. Eight models were successfully established in each group. the sham operation group and model group were given conventional feed. Oxymatrine were gavage administration 0.5 ml/100 g, once a day, lasted for 4 weeks. Respectively in postoperative week, and three weeks, to observe the Oxymatrine on cardiac output (CO), left ventricular end systolic pressure (LVESP), left ventricular end-diastolic pressure (LVEDP), left indoor pressure change rate peak (dp/dtmax)), and left ventricular cavity internal diameter (D), ventricular weight index (VWI), ventricular weight (VW).

RESULTSLeft ventricular anterior wall was from red to deep purple, electrocardiogram showed II guide ST-segment camber up ≥ 0.25 mv. Postoperative week in Oxymatrine group compared with model group, LVESP increased significantly (P < 0.01), LVEDP decreased obviously (P < 0.01); After three weeks in Oxymatrine group compared with model group, VW, VWI decreased (P < 0.05), D significantly reduced (P < 0.01); LVESP increased significantly (P < 0.01), LVEDP decreased obviously (P <0.01); dp/dt(max), CO increased (P < 0.05).

CONCLUSIONAfter acute myocardial infarction in rabbit Oxymatrine can improve the left ventricular reconstruction parameters, increase cardiac output, and improve cardiac function.

Alkaloids ; pharmacology ; Animals ; Cardiac Output ; Heart ; drug effects ; Myocardial Infarction ; pathology ; Quinolizines ; pharmacology ; Rabbits ; Ventricular Remodeling ; drug effects

Objective To study the recent clinical efficacy of compound matrine injections combined with three-dimensional conformal intensity modulated radiotherapy in the treatment of locally advanced pancreatic cancer.Methods 48 cases of untreated locally advanced pancreatic cancer were randomly divided into treatment group (compound matrine injections combined with three-dimensional conformal intensity modulated radiotherapy) and control group (three-dimensional conformal intensity modulated radiationtherapy),there were 24 cases in each group.Three-dimensional conformal intensity modulated radiation therapy used 95 ％ isodose coverage PTV.Using 6 MV X-ray irradiation,2 Gy/day,5 times/week,the total radiation dose was 50 Gy/25 times/5 weeks to complete.Treatment group:one week before treatment infusion of compound matrine injections 20 ml/time,l/d,21-day was one cycle.Control group:simple intensity modulated radiotherapy.Results After treatment,the clinical benefit response rate of the treatment group was 87.5 ％ (21/24),the control group was 70.83 ％ (17/24),the difference was statistically significant (P =0.045).Term efficacy rate of the treatment group was 79.17 ％ (19/24),the control group was 50.00 ％ (12/24),the difference was statistically significant (P =0.035).Adverse reactions were hematologic toxicity,leukopenia occurred in the treatment group was 16.67 ％ (4/24),but the control group was 45.83 ％ (11/24),the difference was statistically significant (P =0.029).Conclusion The effects of compound matrine injections combined with three-dimensional conformal intensity modulated radiotherapy for locally advanced pancreatic cancer are good,they can relieve pain,improve short-term efficacy,reduc side effects,and improve the quality of life.

Objective:To explore the value of clinical pharmacist in clinical treatment through the pharmaceutical care on a patient with Japanese B encephalitis complicated with pulmonary infection. Methods:Clinical pharmacist participated in the whole treatment process and gave suggestions on the selection,dosage and course of the drugs prescribed for anti-virus, anti-epilepsy and anti-infection by focusing on the drug interactions and adverse reactions. Results:The treatment course of the patient was smooth, and the pathoge-netic condition was brought under control gradually while no obvious adverse drug reactions occurred. Conclusion:The work of clinical pharmacist can help to optimize treatment programs to ensure the safety and effectiveness of medication.

Objective: To investigate the relationship between the expression of Raf-1 and tumor angiogenesis,and to discuss its clinical significance.Methods: Tissue microarray technique was used to detect the expression of Raf-1 in 87 specimens of human colon carcinoma,their corresponding adjacent tissues,and incision margins.The patients were from the Department of Pathology of Xijing Hospital between 2005 and 2006.Microvessel density(MVD) was detected using immunohistochemistry with CD34 labeling.The correlation between Raf-1 expression and MVD with the tumor size,metastasis,and differentiation was analyzed.Results: The positive rates of Raf-1 in colon carcinoma tissues,adjacent tissues and incision margins were 86.47%,37.34% and 11.03%,respectively;there were significant difference among the 3 values(P

Objective:To investigate the efficacy of gastrodin injection combined with dexamethasone in the treatment of sudden deafness and its effect on hemorheology and hearing.Methods:Eighty patients with sudden deafness who received treatment in the Department of Otolaryngology, General Hospital of Taiyuan Iron and Steel (Group) Co., Ltd., from August 2018 to March 2020 were included in this study. These patients were randomly assigned to receive either intratympanic injection of dexamethasone (control group, n = 40) or intratympanic injection of dexamethasone combined with gastrodin injection (treatment group, n = 40). Total effective rate was compared between the two groups. The auditory threshold at 0.5, 1.0, 2 and 4 kHz was measured in each group. Before and after treatment, plasma viscosity, low shear whole blood viscosity, fibrinogen and erythrocyte deformability index were compared between the two groups. Drug-related adverse reactions were recorded in each group. Results:Total effective rate in the treatment group was significantly higher than that in the control group [92.50% (37/40) vs. 75.00% (30/40), χ2 = 4.401, P < 0.05]. After treatment, the auditory threshold at 0.5, 1.0, 2 and 4 kHz was significantly decreased compared with before treatment in each group (all P < 0.001). After treatment, the auditory threshold at 0.5, 1.0, 2 and 4 kHz was (42.71 ± 7.06) dB, (44.01 ± 9.35) dB, (48.06 ± 11.37) dB and (52.40 ± 14.26) dB, respectively, in the treatment group and (50.14 ± 8.09) dB, (53.70 ± 10.46) dB, (55.10 ± 13.47) dB and (59.26 ± 15.77) dB respectively in the control group. There were significant differences between the two groups ( t = 4.376, 4.368, 2.526 and 2.267, all P < 0.05). After treatment, plasma viscosity, low shear whole blood viscosity and fibrinogen in each group were significantly decreased (all P < 0.001). After treatment, erythrocyte deformability index in each group was significantly increased ( P < 0.001). After treatment, plasma viscosity, low shear whole blood viscosity and fibrinogen were significantly decreased, and erythrocyte deformability index was significantly increased, compared with before treatment in each group. After treatment, plasma viscosity, low shear whole blood viscosity and fibrinogen, and erythrocyte deformability index were (1.68 ± 0.39) mPa · s, (19.07 ± 3.65) mPa · s, (3.11 ± 0.58) g/L, (0.68 ± 0.08), respectively in the treatment group, and (2.01 ± 0.41) mPa · s, (22.10 ± 4.49) mPa · s, (3.89 ± 0.75) g/L, (0.60 ± 0.07) respectively in the control group. There were significant differences in plasma viscosity, low shear whole blood viscosity and fibrinogen, and erythrocyte deformability index between the two groups ( t = 3.688, 3.312, 5.203, 4.760, all P < 0.05). There was no significant difference in the incidence of adverse reactions between the treatment and control groups (10.00% vs. 15.00%, χ2 = 0.457, P > 0.05). Conclusion:Gastrodin injection combined with dexamethasone can enhance the curative effect on sudden deafness, effectively improve patient's hemorheology and hearing, and is highly safe.