Objective To evaluate the therapeutic efficacy of percutaneous transhepatic portal vein catheterization and thrombolysis on acute superior mesenteric vein thrombosis.Methods The treatment and therapeutic efficacy of 7 cases of acute superior mesenteric vein thrombosis underwent percutaneous transhepatic portal vein catheterization and thrombolysis under ultrasound guidance from August 2005 to April 2009 were analyzed.Results All the patients succeeded in portal vein catheterization and no bile leakage or abdominal bleeding occurred during the procedure.The clinical symptoms such as abdominal pain,abdominal distension,and passing bloody stool relieved were relieved and liquid diet began at postoperative of day 2-5.Emergency operation was done in one case and there was no intestinal fistula.The angiography after the operation showed that the majority of thrombosis were cleared and the blood of portal vein and superior mesenteric vein flowed smoothly.During the follow-up of 3 months to 3 years,all the patients’ status maintained well and no recurrence occurred.Conclusion Treatment of acute superior mesenteric vein thrombosis by percutaneous transhepatic portal vein thrombolysis is safe and effective.

The separation and analysis of hematoporphyrin photosensitizers PSD-001, photocarcinorin,photofrin II and other HPD preparations in comparison with authentic samples protoporphyrin (PP), 3-(8)-(1-hydroxyethyl) -8-(3-)-vinyldeuteroporphyrin ( HVD)and hematoporphyrin(HP) using thin layer chromatography(TLC) under different conditions are described here.It has been shown that there are 3 and 2 main spots in PSD-001 and photocarcinorin by common TLC, but 8-9 spots by high performance TLC.It has been found by comparison with authentic HP,HVD and PP that the amount of PP in PSD-001 or photocarcinorin is less than 3% and HVD,about 2.5% and 6.3% respectively,the HP content of photocarcinorin is much lower than other photosensitizers mentioned above and there is not any component near the origin spot.The Rf of one of the major components of photocarcinorin is 0.65.This component seems to be hardly found in other photosensitizers.These suggest that photocarcinorin is a new photosensitizing agent differing from HPD and photofrin II.

OBJECTIVE:To establish a RP-HPLC method for the determination of seratrodast concentration in human plasma.METHODS:The sample was determined on Lichrospher C18 column with the mobile phase consisted of 0.02 mol?L-1 potassium dihydrogen phosphate(containing 0.1% triethylamine,pH=5.0)-acetonitrile(70∶30)at a flow rate of 1.0 mL?min-1.The column temperature was 30℃ and the detective wavelength was set at 268 nm.RESULTS:The linear range of seratrodast was 28～5 600 ng?mL-1(r=0.999 5)with the lowest detection concentration at 28 ng?mL-1.The recovery rates of seratrodast at low,middle and high concentrations were 102.9%,100.4% and 99.7%,respectively;and both intra-day RSD and inter-day RSD were all less than 6%.CONCLUSION:The method developed in this study is applicable for the determination of plasma concentration and pharmacokinetic study of seratrodast.

The essay discussed on how traditional Chinese medicine (TCM) recognizes the mechanism of fatigue stage from three organs of liver, spleen and heart. TCM believes the mechanism of fatigue in sub-health lies in stagnation of liver Qi due to emotional disorder and deficiency both Qi and blood due to overexertion hurting heart and spleen. Stagnation of liver Qi plays an leading role in the occurrence and development of fatigue state, while the strain of heart and spleen is the important manifestation of this state.

BACKGROUND: Fiber posts are currently perceived as promising alternatives to cast metal posts, as their elastic modulus are similar to that of dentin, producing a favorable stress distribution and providing more esthetic outcomes for endodontically treated teeth. Failure of a fiber post and composite resin core often occurs at the junction between the two materials. This failure process requires better characterization.OBJECTIVE: To compare the bond strength of fiber posts after three surface treatments to a composite resin. METHODS: The coronal portions of 20 mandibular premolars with a single root canal were removed and were endodontically treated. After post space preparation, the roots were divided into four groups and the fiber posts and resin cores were lured. In the three testing groups, the surfaces of fiber posts were silaned, sandblasted or etched with hydrogen peroxide. In the control group, the surfaces of fiber posts were not treated. All specimens were exposed to cyclic loadings and thermal cycles. The roots were sectioned into slices with 1 mm thickness and their bond strengths were tested with a universal material testing machine. Test piece following testing was placed under optical microscope to observe the breakage method.RESULTS AND CONCLUSION: Stickiness strength was significantly greater in the sandblasted and hydrogen peroxide groups compared with the control group (P < 0.05). No significant difference in stickiness strength was determined between the sandblasted and hydrogen peroxide groups (P > 0.05). No significant difference in stickiness strength was detected between the silaned and control groups (P > 0.05). The splicing destruction among each group was the main breakage method between materials and fiber posts. The surface treatment of fiber posts with sandblast or hydrogen peroxide significantly enhanced the bond strength of the composite resin tested. The surface treatment of fiber posts with silane did not enhance the bond strength of the composite resin.

Objective To explore the effect of aging on nonspatial learning and memory ability or recognitive memory ability in C57BL/6 mice.Method The C57BL /6 mice were used and novel-object recognition task was used to test the recognition memory ability.Results There was hardly exploring behavior in half of mice in the novel-object recognition task.Conclusion The novel-object recognition task is valueless to evaluate the effect of age on the nonspatial learning and memory ability in the C57BL/6 mice.

Objective To explore whether there is the decline of learning and memory ability in C57BL/6 mice in radial six-arm water maze (RAWM) task. Methods The RAWM task was used to test the spatial abilities of learning and memory. Results All of old mice showed the decline of spatial learning and memory ability in the RAWM task. Conclusion RAWM task is excellent spatial learning and memory task and is sensitive to measure the age-related decline of learning and memory ability in C57BL/6 mice.

Cumene Hydroperoxide(CHP)-induced mouse mitochondrial lipid peroxides (LPO) are significantly higher than those of the normal mitochondria ( P

The fatty acid content of liver mitochondria was quantitatively determinde with gas chromatography in rats after they were inflicted with 15% TBSA full thickness burns or after the liver mitochondria were incubated with lipids extracted from eschar tissue(D1)or from normal skin of rats(D'1).It was found that after burn injury,the content of unsaturated fatty acid of liver mitochondria decreased and that of saturated fatty acid increased significantly.Both Dl and D'l decreased the content of unsaturated fatty acid and the decrease would be more significant when the concentration of eschar lipids or skin lipids was increased.D1 reduced the unsaturated fatty acid content more significant than D'1 when the two were in same concentration.The possible mechanism of the decrease of unsaturated fatty acid in the liver mitochondria induced with burn injury,eschar lipids or normal skin lipids was discussed briefly.

Objective:To study the inhibitory effect of tumor-selective replication-competent adenovirus-mediated human endostatin(CX-hE)on transplanted ovarian cancer(OV-90) in nude mice.Methods: BALB/c nude mice were inoculated subcutaneously with OV-90 cells to establish the animal model bearing human ovarian cancer.Eighteen nude mice bearing cancer were divided into 3 groups to receive intratumoral injection of PBS,CX-hE or Ad-hE,1/2 d,5 times.Then their livers were harvested for pathologic examination.Another 15 nude mice were divided into 3 groups to receive single intratumoral injection of CX-hE,Ad-hE or ONYX-015.Venous blood was collected on day 1,3 and 7 after injection for hEndo measurement by ELISA.The tumors were harvested for pathologic examination and immunohistochemical staining.Results: The tumors grew more slowly in CX-hE group and their sizes were markedly smaller than those of Ad-hE group(P