1.Research of expression of aldehyde dehydrogenase 1 and breast cancer resistance protein and their correlation in breast cancer
Xing WANG ; Zhen JIANG ; Changgang SUN
China Oncology 2014;(3):175-181
Background and purpose:Resistance to antitumor agents is a major cause of treatment failure in patients with breast cancer. Research has shown that, tumor stem cell marker aldehyde dehydrogenase 1 (ALDH1) is related with some anticancer drugs (such as cyclophosphamide, cisplatin) resistance, and the content of ALDH1 in tumor cells after treatment is higher than that before treatment. Breast cancer resistance protein (BCRP) is not expressed in normal tissues, but high expressed in breast cancer of after treatment, it may be associated with the mechanism of tumor drug resistance. This study was to investigate the correlation between expression and the relationship between these two kinds of protein ALDH1, BCRP and clinical pathological characteristics. Methods:Immunohistochemistry was performed to detect the expression of ALDH1 and BCRP in breast inifltrating ductal carcinoma tissues, and whether there is a correlation between and explore their relationship with clinical pathological features and their expression. Results:The expression of ALDH1 protein and BCRP protein in breast cancer and paracarcinoma breast tissues has signiifcant difference(χ2=14.685, P=0.000;χ2=12.243, P=0.000).The expression of ALDH1 with patients age, pathologic stage, axillary lymph node metastasis, histological grading, ER, PR, and HER-2 state were not relevant(P>0.05). HER-2, BCRP protein, expression was higher in cancer tissue (χ2=5.289, P=0.021). There were no relevant with the expression of BCRP with patients age, pathologic stage, axillary lymph node metastasis and histological grading, estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor (HER-2) (P>0.05). Conclusion:ALDH1 proteins may be an independent factor compared with occur drug resistant protein, and participate breast cancer drug resistance in the chemotherapy and tumor invasion and metastasis of malignant biological behavior.
2.One case of left atrial myxoma complicated with systemic multiple vascular thrombosis.
Xing-zhen SUN ; Xiang-yang TIAN ; Juan LIU
Chinese Journal of Pediatrics 2013;51(7):548-548
Brain Infarction
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diagnosis
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etiology
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therapy
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Cerebral Angiography
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Child
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Echocardiography, Doppler, Color
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Heart Atria
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Heart Neoplasms
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complications
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diagnosis
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surgery
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Humans
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Intracranial Embolism
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diagnosis
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etiology
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therapy
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Male
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Myxoma
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complications
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diagnosis
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surgery
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Pulmonary Edema
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diagnosis
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etiology
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therapy
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Thrombosis
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diagnosis
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etiology
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therapy
5.Comparison of the clinical effects of transurethral plasmakinetic enucleation of prostate and suprapubic transvesical prostatectomy in the treatment of large volume benign prostatic hyperplasia
Wei CHEN ; Xing LIU ; Xin ZHEN ; Fanghao SUN
Clinical Medicine of China 2017;33(7):639-642
Objective To assess the clinical efficacy and safety of transurethral plasmakinetic enucleation of prostate (PKEP) and suprapubic transvesical prostatectomy (SPP) in the treatment of large volume benign prostatic hyperplasia (BPH).Methods A total of eighty patients with large volume BPH were selected in the First People''s Hospital of Xuzhou from March 2014 to December 2016,and they were randomly divided into two groups,the PKEP group and SPP group,with 40 cases in each group.The comparison was made between the two groups in terms of operation indicators,postoperative complications followed up for 6 months after surgery and other related curative effect indexes.Results In the SPP group,the intraoperative blood loss was significantly higher than that of the PKEP group ((215.7±10.4) ml vs.(75.6±9.2) ml,t=6.541,P<0.05).There was no significant difference between the two groups in operation time and excised gland volume (P>0.05).But the catheterization time after operation,bladder irrigation time and length of hospital stay were significantly longer in the PKEP group than in the SPP group ((6.3±1.8) d vs.(2.4±0.6) d,(5.5±1.4) d vs.(1.3±0.6) d,(7.7±2.3) d vs.(3.1±0.7) d,t=4.357,2.542,2.975,P<0.05);in the 6-month follow-ups after operation,IPSS,Qmax,PVR and QOL were all significantly improved in both groups compared with the indexes collected before the operation (P<0.05),while there was no significant difference between the two groups (P>0.05).The rate of complication occurrence in the PKEP group were lower than that of the SPP group (P<0.05).Conclusion PKEP was effective in the treatment of BPH,with less bleeding loss,fewer complications and quick recovery.It can significantly improve the life quality of the patients and will be an ideal treatment for large volume BPH.
6.Activation of Rho Kinase in Lung Tissue of Hypoxic Pulmonary Hypertension in Rats in Different Stages and Its Significance
xing-zhen, SUN ; xiang-yang, TIAN ; da-wei, WANG
Journal of Applied Clinical Pediatrics 1986;0(01):-
Objective To study the expression of Rho kinase and its functional activation in lung tissue from hypoxic pulmonary hypertension(HPH) rat model,and the effects of fasudil on HPH.Methods Seventy-two male Spraque-Dawley rats were randomly divided into the control group,hypoxic model group,and fasudil-intervention group[group with hypoxia and fasudil for 15 mg/(kg?d)],respectively.Mean pulmonary arterial pressure(mPAP) and right ventricle hypertrophy index(RVHI) were measured.Expression of Rho kinase mRNA and protein were examined by reverse transcriptase-polymerase chain reaction(RT-PCR) and Western blot,respectively.The phosphorylation of binding subunit of myosin phosphatase(MBS)-a substrate of Rho kinase was detected by Western blot and defined,as the mark of functional activation of the kinase.Results The expression of Rho kinase mRNA in hypoxic model group was markedly upregulated even before the onset of the third day after the experiment(HPH),and it was much lower in rats of fasudil group than that of hypoxic model group.The phosphorylation of MBS was significantly higher in hypoxic model group than that in control group,and it was positively correlated with the mPAP and RVHI(all P
7. Value of CT texture analysis in the preoperative prediction of Fuhrman grade of clear cell renal cell carcinoma
Jiule DING ; Zhaoyu XING ; Zhen CHEN ; Shengnan YU ; Jun SUN ; Jie CHEN ; Jianguo QIU ; Wei XING
Chinese Journal of Radiology 2018;52(8):614-618
Objective:
To detect the values of CT texture features in the preoperative prediction of Fuhrman grade of clear cell renal cell carcinoma (ccRCC).
Methods:
The CT data of 206 patients with ccRCC admitted to the Third Affiliated Hospital of Soochow University from January 2011 to December 2016 were retrospectively analyzed, and the ccRCC cases were graded using Fuhrman grading system, including 38 cases of Grade Ⅰ, 107 cases of Grade Ⅱ, 50 cases of Grade Ⅲ and 11 cases of Grade Ⅳ. All subjects undergone plain and enhancement CT scans. There were two methods used for the extraction of texture features, including histogram (2 features: Kurtosis and Skewness) and gray-level co-occurrence matrix (6 features: Contrast, Correlation, Energy, Entropy, Homogeneity and Variance). Each texture feature during Grade Ⅰ to Ⅳ was compared using a one-way analysis of variance following the log-ratio transformation, and a Newman-Keuls test was performed for all pairwise comparisons. An independent sample
8.Discrimination of patients with Xiao-Chaihu Tang syndrome using 1H NMR metabonomics and partial least square analysis.
Jie XING ; Shu-chun YUAN ; Hui-min SUN ; Ma-li FAN ; Zhen-yu LI ; Xue-mei QIN
Acta Pharmaceutica Sinica 2015;50(8):1032-1037
1H NMR metabonomics approach was used to reveal the chemical difference of urine between patients with Xiao-Chaihu Tang syndrome (XCHTS) and healthy participants (HP). The partial least square method was used to establish a model to distinguish the patients with Xiao-Chaihu-Tang syndrome from the healthy controls. Thirty-four endogenous metabolites were identified in the 1H NMR spectrum, and orthogonal partial least squares discriminant analysis showed the urine of patients with Xiao-Chaihu Tang syndrome and healthy participants could be separated clearly. It is indicated that the metabolic profiling of patients with Xiao-Chaihu Tang syndrome was changed obviously. Fifteen metabolites were found by S-pot of OPLS-DA and VIP value. The contents of leucine, formic acid, glycine, hippuric acid and uracil increased in the urine of patients, while threonine, 2-hydroxyisobutyrate, acetamide, 2-oxoglutarate, citric acid, dimethylamine, malonic acid, betaine, trimethylamine oxide, phenylacetyl glycine, and uridine decreased. These metabolites involve the intestinal microbial balance, energy metabolism and amino acid metabolism pathways, which is related with the major symptom of Xiao-Chaihu Tang syndrome. The patients with Xiao-Chaihu Tang syndrome could be identified and predicted correctly using the established partial least squares model. This study could be served as the basis for the accurate diagnostic and reasonable administration of Xiao-Chaihu-Tang syndrome.
Humans
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Least-Squares Analysis
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Medicine, Chinese Traditional
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Metabolome
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Metabolomics
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Proton Magnetic Resonance Spectroscopy
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Syndrome
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Urinalysis
9.Compare three methods to detect the Pneumocystis carinii in the bronchoalveolar wash sample of AIDS patients.
Liang ZHANG ; Xing-wang LI ; Bing SHEN ; Xiao-ying TENG ; Lei SUN ; Zhen-wei LANG ; Ping YANG ; Peng WANG
Chinese Journal of Pathology 2011;40(7):482-484
AIDS-Related Opportunistic Infections
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diagnosis
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microbiology
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Acquired Immunodeficiency Syndrome
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diagnosis
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microbiology
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Bronchoalveolar Lavage Fluid
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microbiology
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Female
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Humans
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Immunohistochemistry
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Male
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Methenamine
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Pneumocystis carinii
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isolation & purification
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Pneumonia, Pneumocystis
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diagnosis
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microbiology
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Polymerase Chain Reaction
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Silver Staining
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methods
10.The differential expression profile of polycystic ovary syndrome associated genes
Zhen-Xing HU ; Jie QIAO ; Mei-Zhi LI ; Xiao-Wei ZHANG ; Yong-Jian CHEN ; Rong LI ; Chun-Ling SUN ;
Journal of Peking University(Health Sciences) 2003;0(06):-
Objective:To explore the gene differential expression pattern of polycystic ovary syn-drome.Methods:We carried out microarray analysis to define the gene networks by the PCOS granulosacells in order to identify differentially expressed genes in PCOS patients.These granulosa cells of fivePCOS cases and five control cases which were derived during oocyte retrieval from women undergoingIVF.Results:As compared with control human ovarian granulosa cells,46 genes were screened out,25genes were up-regulated,and 21genes were down-regulated in PCOS.These differentially expressedgenes were involved in various biologic functions,such as regulation of fatty acid metabolism,cell-cellsignal transduction,immune and inflammatory response,reflecting the complexity of clinical manifesta-tions of PCOS.Conclusion:Microarray analysis technology is an effective mothod to identify novel PCOSassociated candidate genes.