fluconazole.Conclusion: The susceptibilities of Malassezia species to ketoconazole,itraconazole,and fluconazole are different.Microdilution method from M27-A is suitable for testing the susceptibility of Malassezia in vitro.

Objective To analyze clinical features of patients with eosinophilia-myalgia syndrome (EMS).Methods Clinical manifestations,response to treatment and prognosis of 89 cases with eosinophilia hospitalized at Peking Union Medical College Hospital(PUMCH)during the past nine years were analyzed retrospectively,including four cases conformed with the diagnostic criteria for EMS set by the Centers for Disease Control and Prevention of the US.Results Infection,tumor and other diseases that could cause increased eosinophil count were excluded in all but four cases,with all female,average age of 48.8 years,and mean count of eosinophil of 42.75 percent of blood cells.Multi-organs and systems were involved in the patients,with the most common clinical manifestations of myalgia,skin rash,joint swelling,arthralgias,respiratory symptoms and paresthesia.Patients responded well to prednisone at acute phase of illness,but prognosis was poor as severe pulmonary hypertension occurred.Occurrence of EMS closely associated with environmental factors and special attention should be paid to patient's history of medication and diet in its diagnosis.Conclusion EMS is characterized by the increasing of eosinophils and myalgia,while multi-organs and systems were involved.Glucocorticoid plays an important role in the treatment at acute phase of illness.

Objectives:To understand the patients' cognition and satisfaction with public welfare in public hospitals.Methods:A questionnaire survey was conducted among 600 patients in Beijing municipal public hospitals and the data was statistically analyzed by SPSS 22.0.Results:Patients' understanding of public welfare was not ideal,the satisfaction was quite well,and the satisfaction of the patients in the experimental hospitals was more satisfactory than those in non-experimental hospitals.The patients have urgent needs on rational drug use,mutual recognition of medical results,smooth channels of patient rights,reduced medical expense and shortened waiting time.Conclusions:It should strengthen the publicity and governance of the public welfare of public hospitals to enhance the patients' understanding,breakthrough the urgent needs of public welfare to improve patients' satisfaction,and intensify the policy linkage and explore diversified implement forms of public welfare to improve service level.

Dual antiplatelet therapy is the standard treatment for patients with acute coronary syndrome (ACS) and coronary heart disease interventional treatment. Clopidogrel, a traditional antiplatelet agent, has some disadvantages, such as slow onset time, individual differences and dissatisfy the antithrombosis requirement in ischemia of high-risk patients. In addition, for ACS patients with indications of anticoagulation, antiplatelet therapy cannot prevent venous thromboembolic events. However, dual antiplatelet combined with anticoagulant therapy may decrease the risk of ischemic events at the price of increasing bleeding. With the development of new antithrombotic agents, the antithrombotic strategy for ACS has made some progress, such as antiplatelet strategy for ACS, antithrombotic strategy for ACS with indication of anticoagulation and new antithrombotic drugs. With the development of clinical antithrombotic drug research, the patients with ACS will benefit from the optimized strategy of combined with antithrombotic therapy.

Liver cancer remains difficult to treat, owing to a paucity of drugs that target critical dependencies; broad-spectrum kinase inhibitors such as sorafenib provide only a modest benefit to patients with hepatocellular carcinoma. The induction of senescence may represent a strategy for the treatment of cancer, especially when combined with a second drug that selectively eliminates senescent cancer cells (senolysis). Here, using a kinome-focused genetic screen, we show that pharmacological inhibition of the DNA-replication kinase CDC7 induces senescence selectively in liver cancer cells with mutations in TP53. A follow-up chemical screen identified the antidepressant sertraline as an agent that kills hepatocellular carcinoma cells that have been rendered senescent by inhibition of CDC7. Sertraline suppressed mTOR signalling, and selective drugs that target this pathway were highly effective in causing the apoptotic cell death of hepatocellular carcinoma cells treated with a CDC7 inhibitor. The feedback reactivation of mTOR signalling after its inhibition is blocked in cells that have been treated with a CDC7 inhibitor, which leads to the sustained inhibition of mTOR and cell death. Using multiple in vivo mouse models of liver cancer, we show that treatment with combined inhibition of CDC7 and mTOR results in a marked reduction of tumour growth. Our data indicate that exploiting an induced vulnerability could be an effective treatment for liver cancer.

Acupuncture Points ; Administration, Cutaneous ; Adult ; Aged ; Drugs, Chinese Herbal ; administration & dosage ; Facial Paralysis ; drug therapy ; Female ; Humans ; Male ; Middle Aged ; Treatment Outcome ; Young Adult

Carcinoma, Squamous Cell ; diagnosis ; Humans ; Maxilla ; pathology ; Maxillary Neoplasms ; diagnosis ; Neoplasm Metastasis ; Spinal Neoplasms ; secondary

Objective: To compare the advantages and disadvantages of the intraluminal suture, electrocautarization of middle cerebral artery combined with unilateral ligation of common carotid artery and electrocautarization of middle cerebral artery combined with bilateral ligation of common carotid artery for establishment of focal cerebral ischemia models in rats. Methods: The focal cerebral ischemia rat models were induced by 3 means. Twenty rats were randomly allocated into intraluminal suture group (n = 6), electrocautarization with unilateral ligation group (n = 7), and electrocautarization with bilateral ligation group (n = 7) according to the different methods for establishment of the rat model. A laser Doppler flow metry was used to detect the relative values of the cerebral blood flow before and after cerebral infarction. The neurological deficit scores of models were measured after 24 h, and the brain slices were stained with 2,3,5-triphenyl tetrazolium chloride. The volume of cerebral infarction was calculated. Results: Circled digit oneThe neurological deficit scores in the intraluminal suture group (9.6 ± 0.6) and the electrocautarization with bilateral ligation group (9.8 ± 0.6) were higher than those in the electrocautarization with unilateral ligation group (5.7 ± 0.7). The differences were statistically significant (P < 0.01). Circled digit twoThe volume of cerebral infarction in the intraluminal suture group (131.5 ± 15.8) mm3 and the electrocautarization with bilateral ligation group (141.7 ± 19.8) mm3 were larger than that in the electrocautarization with unilateral ligation group (39.9 ± 11.4) mm3. The differences were statistically significant (P < 0.01). Circled digit threeThe relative value of cerebral blood flow (CBF) in the electrocautarization with bilateral ligation group was lower than that in the electrocautarization with unilateral ligation group. The difference was statistically significant (P < 0.05). After reperfusion, the relative value of CBF in the intraluminal suture group was higher than that in the electrocautarization with bilateral ligation group. The difference was statistically significant (P < 0.05). Conclusion: The three methods had their advantages and disadvantages in inducing focal cerebral ischemia model in rats. A different model should be used according to the purposes of research.

Child, Preschool ; Hemophilia A ; classification ; diagnosis ; therapy ; Hemorrhage ; diagnosis ; therapy ; Humans ; Infant

Eustachian Tube ; abnormalities ; surgery ; Humans ; Laser Therapy ; Male ; Middle Aged ; Prosthesis Implantation ; Stents ; Tympanoplasty