Substantial evidence supports the relationship between chronic inflammation and cancer development. Numerous studies suggest that chronic inflammatory disease, such as periodontitis, contributes to head and neck squamous cell carcinoma development. Oral squamous cell carcinoma (OSCC) is the most common malignant tumor in the oral and maxillofacial regions. Porphyromonas gingivalis, one of the most important pathogens in association with periodontal disease, might have a potential correlation with OSCC. Along with the development of molecular biological techniques, the association between Porphyromonas gingivalis and OSCC has been greatly emphasized in recent years. This review summarizes the association between these variables and the potential mechanisms involved in such relationship.
Carcinoma, Squamous Cell ; pathology ; Humans ; Mouth Neoplasms ; pathology ; Periodontal Diseases ; Periodontitis ; Porphyromonas gingivalis ; Research

Humans ; Metabolic Syndrome

Cardiovascular Diseases ; etiology ; prevention & control ; Humans ; Hypertension ; complications ; Risk Factors

Objective To determine the role of SCN1A gene variation in the development of familial febrile seizures (FS).Methods Clinical data were collected from 8 familial FS pedigrees, and peripheral venous blood samples were collected from the probands and other available family members. All 26 coding exons and exon-intron boundaries at least 50 bases of the human SCN1A gene were amplifled by polymerase chain reaction, the products were subsequently sequenced. To novo variation, other family members were screened for the corresponding exons. Two hundred age-matched healthy children were served as normal controls. ResultsA total of 33 variations in the SCN1A gene were identifled in these families. Of these variations, one was a missense mutation; the remaining 32 variations were previously submitted as single nucleotide polymorphisms (SNPs). A c.2650G>A heterozygous missense mutation in exon 15 of the SCN1A gene found in the proband of family 4 was inherited from his father who had seizures with fever in early childhood. The c.2650G>A mutation was absent in the 400 alleles of normal controls. To the best of our knowledge, the SCN1A c.2650G>A mutation has neither been reported in the NCBI SNP database nor in the literature to date. The c.2650G>A mutation changes a glycine at amino acid 884 in the SCN1A protein to a serine (p.Gly884Ser). Protein sequence analysis showed that the p.Gly884Ser is located at a highly conserved region between the 4th and 5th transmembrane segment of the homologous domain Ⅱ of voltage-gated sodium channel 1 subunit (DIIS4-S5). ConclusionsThe pathogenesis of familial febrile seizures was related to the SCN1A variation, the mutation outside the region of the voltage sensor (S4) and ion channel pore (S5-S6) of the voltage gated sodium channelα-subunit may be an important factor to cause mild phenotype epilepsy syndrome.

Objective To compare the clinical characteristics of pregnant women with gestational diabetes mellitus (GDM) and normal gestational glucose metabolism at the 25th weeks of pregnant. Methods Sixty-eighty patients with GDM (GDM group) and 68 patients with normal blood glucose(control group) were enrolled in this study. During 25 weeks of pregnancy, oral glucose tolerance test (OGTT), blood pressure, fasting insulin, glycosylated hemoglobin, uric acid, triglyceride, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol were measured and body mass index (BMI), homeostasis model of assessment for insulin resistence index (HOMA-IR), insulin sensitivity index (ISI) were computed. The results were compared between two groups. Results The age in GDM group was significantly higher than that in control group: (31.38 ± 0.54) years vs. (29.50 ± 0.56) years, P<0.05. The systolic pressure in two groups had no significant difference (P>0.05), but diastolic blood pressure in GDM group was significantly higher than that in control group:(73.2 ± 0.8) mmHg vs. (70.9 ± 0.8) mmHg, 1 mmHg=0.133 kPa, P<0.05. The body weight and BMI in GDM group were significantly higher than that in control group:(65.67 ± 1.76) kg vs. (57.76 ± 1.11) kg, (24.77 ± 0.61) kg/m2 vs. (22.11 ± 0.42) kg/m2, P<0.01. The levels of glycosylated hemoglobin, fasting insulin and HOMA- IR in GDM group were significantly higher than those in control group (5.546 ± 0.746)% vs. (5.085 ± 0.034)% , (17.870 ± 1.015) mU/L vs. (14.400 ± 0.634) mU/L, 4.192 ± 0.271 vs. 2.645 ± 0.128, but the level of ISI in GDM group was significantly lower than that in control group:0.014 ± 0.001 vs. 0.020 ± 0.001, and there were significantly differences (P<0.01). The levels of uric acid, triglyceride, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol in two groups had no significant differences (P>0.05). Conclusions Compared to those with normal blood glucose, the patients with GDM have the characteristics of higher age, higher body weight, higher BMI, higher diastolic blood pressure , higher level of insulin, insulin resistance and decreased insulin sensitivity.

Objective To estimate the general power of OCT and GDX in the detection of glaucoma on the item of retinal nerve fiber layer thickness.Methods 42 patients(80eyes)underwent OCT and GDX.To compare the results.Results There was significant difference between the two methods.but they had significant plus correlation.Conclusions The sensitivity and agreement of GDX in the examination of RNFL is quite good compared with OCT for the detection of glaucoma.

Recent studies indicate that there are expressions of gastrin and its receptors in the tissue and cell line of colorectal cancer .Gastrin stimulates the proliferation of colorectal carcinoma as well as inhibits the apoptosis of colorectal carcinoma so as to promote the growth and invasion of colorectal carcinoma via a series of signal transduction pathways induced by its specific receptors. To inhibit the abnormally active signal transduction pathways that stimulate the proliferation of colorectal carcinoma will provide a new effective measure for the prevention and management of colorectal carcinoma.But the accurate signal transductions are not clear and need further research.

Objective: To investigate the effect of vascular endothelial growth factor-C (VEGF-C) on invasive capability and proliferation of human cholangiocarcinoma cells. Methods: The effect of VEGF-C on FRH0201 was assayed by MTT. The cycle pattern and apoptosis was assayed using flow cytometry. The effect of VEGF-C on homotypic adhesion and metastasis in FRH0201 was examined with 3H-TdR infiltration and Boyden chamber. Results: VEGF-C could enhance the proliferation of FRH0201 in a dose and time dependent manner. And VEGF-C could inhibit cell apoptosis significantly. After cultured for 2 hours with 1, 5, 10ng/ml of VEGF-C, there were more cells in the lower chamber than the control group. After 60, 90, 120 minutes induction by 1ng/ml、5ng/ml、10ng/ml, the cells showed significantly lower homotypic adhesion than that of the control group. Conclusions: VEGF-C could enhance the proliferation of FRH0201 and inhibit cell apoptosis. VEGF-C could decrease homotypic adhesion of FRH0201 and might be a cause of the metastasis.

Objective To study the effects of environmental noise on the health of frontage uptown residents in Fuzhou city. Methods The AWA6218B sound-level meter was used to determine the sound intensity in five frontage uptowns, the testing time was at noon and in the evening, 213 inhabitants were selected to investigated the health effects by the questionnaire. Results The five tested frontage uptowns' Leq were 57.4-67.7 dB(A), which was in the extent of loudness. Some residents considered the noise as the main pollution which impacted their living quality. The noise had the adverse effect on sleeping, emotion, audition, working and study. Conclusion Environmental noise has serious adverse effects on the health of the frontage uptown inhabitants.

Aim To evaluate the effect of Cdk7 silencing on the c ell cycle control, the phosphorylation level changes of Cdk2 and pRb in human he patoblastoma HepG2 cell culture in vitro, and to validate Cdk7 as a novel ta rget for anticancer therapeutics.Method Levels of Cdk7 and the phosphorylation levels of Cdk2 and pRb were measured by Western-blotting. DNA c ontents, cell cycle and apoptosis induced by Cdk7 silencing were analyzed by flo w cytometry and ultrastructural changes of cells were observed with transmission electron microscopy.Result The phosphorylation levels of pRb a nd Cdk2 and the levels of Cdk7 decreased in a concentration-dependent manner wh en the concentration was above 100 nmol?L -1. Indice of cells arrested in G 0/G 1 phases and apoptotic cells increased in a dosage-and time-dependent manner, the difference was significant between Cdk7 ASODN and the sense control (P