Following ideas are proposed for the development of library-holding resources with the need of users as its target according to the background and characteristics of big data, namely with the users-driving development of library-holding resources as its principle, with the users-satisfying resources acquisition method as its direction, and with the users-needing development of library-holding resources as its integration platform.

AlM: To investigate the risk factors of diabetic retinopathy ( DR) in type 2 diabetes patients.METHODS:A total of 380 cases of type 2 diabetes were selected from January 2014 to June 2014, divided into DR group (126 cases) and non-diabetic retinopathy ( NDR) as control group (254 cases), all patients were underwent medical history, physical examinations, laboratory and assistance examinations. The prevalence and single factor and multiple factors of DR were analysed by the logistic regression model.RESULTS: The univariate logistic regression analysis showed that DR was associated with diabetes duration, systolic blood pressure, triglyceride, total cholesterol, low - density lipoprotein, urine protein, intraocular pressure, carotid intima - media thickness, peripheral neuropathy disease was the related risk factors for DR. ln multiple logistic regression analysis, only duration was the related risk factors for DR.CONCLUSlON:The occurrence of DR is the outcome of combined action of multiple factors, duration is an independent risk factor for occurrence of DR.

Objective The present study was aimed at investigating the effects of cholera toxin(CTx) on promoting the regeneration of retinal ganglion cells(RGCs) in hamster retina. Methods After optic nerve (ON) transection, an autologus sciatic nerve (attached graft, AG) was removed and sutured to the proximal stump of the ON. CT X was injected or (and) a small segment of sciatic nerve (SN) inserted intravitrously. Animals were separated into 5 groups:regenerating control group(AG groups and solution groups); AG+CT X groups; AG+SN groups; AG+CT X+SN groups; Effect and Dosage groups; Animals in the former 4 groups were allowed to survive for 2-6 weeks respectively. The regenerated RGCs were labeled retrogradely by granular blue, and the changes in number of regenerating RGCs in each retina were observed under fluorescent microscope. Results The mean numbers of regenerating RGCs in AG+CT X groups were increased and significantly higher than those in AG groups and solution groups at each time point( P

Detection of high-risk human papillomavirus ( HPV) gene is essential in cervical cancer screening.This paper presents some HPV detection methods according to different molecular techniques , mainly introducing the commonly used hybird capture Ⅱ, HybriMax and method based on fluorescent quantitative PCR , elaborates their advantages and shortcomings , and discusses the clinical applications in the future.

【Objective】 To examine the regeneration of NPY-immuno reactivity (IR) retinal ganglion cells (RGCs) and the effects of cholera toxin ( CTx) injected or/and peripheral nerve implanted on regeneration of NPY-IR RGCs. 【Methods】 16 adult golden hamsters were ramdomly divided into 4 groups. Optic nerve (ON) was transacted and a segment of autologous sciatic nerve (attached g raft, AG) was removed and sutured to the proximal stump of ON in regenerating co ntrol group (AG group). The animals in experimental groups were further treated with CTx injection or/and implantation of a short of segment sciatic nerve (SN) intravitrously. By using the retrograde labeling with fluorogold (FG) combined w ith fluorescent immunocytochemistry, the regenerated NPY-IR RGCs were observed and counted under fluorescent microscope. 【Results】 At 4 weeks after surgery, the mean number of regenerated NPY-IR RGCs per retina in AG group was 58±22 wh ich constitutes (3.36±0.65)% of the total regenerated RGCs. In AG+CTx, AG+SN and AG+CTx+SN experimental groups, the mean numbers of regenerated NPY-IR RGCs per retina were 143±47, 125±37 and 437±77 ordinally which constitute (5.15± 0.89)%, (5.34±0.37)% and (8.11±0.85)% of the total regenerated RGCs respec tively, which were increased significantly compared with those in AG group. 【Co nclusion】 The results show that the axotomized NPY-IR RGCs have th e capability of regenerating their axons into the attached PN graft, CTx and/or SN could enhance the regeneration of the NPY-IR RGCs.

AIM: To explore the main risk factors related to the incidence of endophthalmitis in patients after cataract surgery in China and to provide evidence for prevention.METHODS: The results of 5 studies on the main risk factors of endophthalmitis in patients after cataract surgery were analyzed by Meta-analysis method.
RESULTS: The pooled odds ratio values and 95% CI of age(≥70), diabetes, vitreous overflow, operative time ( ≥ 10min ), common operating room and control of using time of topical anesthetic were 1. 81(95% CI: 1. 43-1. 69),3. 66 (95% CI: 1. 64 - 8. 16),2. 21 (95% CI: 1. 46 -3. 32),3. 54 (95% CI: 2. 47 - 5. 06),2. 77 (95% CI: 2. 07 -3. 72),2. 09(95% CI: 1. 53-2. 86).
CONCLUSION: The main risk factors of endophthalmitis were the age(≥70), diabetes, vitreous overflow, operative time(≥10min), common operating room and control of using time of topical anesthetic.

ObjectiveTo discuss the clinical diagnosis, treatment and genetic diagnosis of congenital chloride diarrhea (CCD), a rare autosomal recessive disease.Methods One month old boy with persistent diarrhea, hypochloremia, hyponatremia, hypokalemia and metabolic alkalosis, his stool electrolyte testing, clinical treatment and follow-up, as well as his and his parents’ SLC26A3 gene mutation analysis were retrospectively analyzed.Results The fecal electrolyte testing showed that the levels of Cl- and K+ were increased and the level of Cl- was much higher than the sum of Na+ and K+. After replacement therapy with NaCl and KCl, the blood electrolyte recovered to normal. Follow-up 4 years, the boy had a normal growth and development. Mutation analysis onSLC26A3 gene showed there was a homozygous mutation of 239G>A and both his father and mother carried the same heterozygous mutation. This mutation was ifrst discovered in China.Conclusions The sequencing analysis ofSLC26A3 mutation may help to diagnosis CCD.

Gastrointestinal stromal tumor(GIST) is the most common mesenchymal malignancy of the GI tract. The mainstay of treatment is surgery. For patients in whom complete resection is not possible, or in patients with metastatic or recurrent disease, they are unresponsive to standard chemotherapy and to radiotherapy. There has been no effective systemic treatment for unresectable GIST or metastatic disease. Gain-of-function mutations of the KIT proto-oncogene occur in up to 90% of GIST, allowing constitutive activation of tyrosine kinase (i.e. auto-phosphorylation of tyrosine residues independent of ligand-receptor binding), leading to aberrant cell division and tumour growth. Imatinib selectively inhibits the tyrosine kinase activity associated with KIT, which forms the rationale for evaluating its effects in GIST. Herein, we review recent developments in treatment for GIST and implication for optimal treatment in these patients.

Objective: To study the relationship between NO, NOS and TNF-? of serum and surgical infection. Methods: We used chemistry technique and ELISA to study serum NO, NOS and TNF-? in 30 cases of surgical infection.Results: Compared with normal group and before treatment group NO, NOS and TNF-? were singnificantly higher in surgical infection(P

Objective To investigate the clinical efficacy and safety of laparoscopic appendectomy for acute appendicitis.Methods Nighty-two cases of acute appendicitis were included in this study,randomly divided into observation group (n =46) and control group (n =46) according to the random number table method.The observation group was treated with laparoscopic appendectomy,and control group with the traditional open appendectomy treatment.We compared the two groups of cases in postoperative conditions and the level of WBC changes and 1d and 3d after operation.Results The operation time of the observation group was shorter than that of the control group (t=4.049,P＜0.05);there was no significant difference in lymph nodes (t=0.570,P＞0.05);the amount of bleeding was less than that of the control group (t=20.748,P＜0.05).The get-out-of-bed activity time in the observation group was faster than the control group (t=9.221,P＜0.05);eating time was faster than the control group (t=8.794,P＜0.05);the anus recovery exhaust time was faster than the control group (t=17.423,P＜0.05);hospital stay was shorter than the control group (t=13.479,P＜0.05);the complication rate was lower than that of the control group (t=13.883,P＜0.05).The levels of 1d and 3d WBC were lower in the observation group than that in the preoperative group (t=7.277,26.911,P＜0.05);the levels of 1d and 3d WBC in the observation group were lower than those in the control group at the same time (t=3.6189,17.827,P＜0.05);the 3d WBC level of the observation group was lower than that of 1d (t=20.732,P＜0.05).Conclusion Laparoscopic appendectomy is a safe and effective treatment for patients with acute appendicitis,and it is worthy of further clinical research.