1.Occurrence of Castleman disease secondary to the treatment of solitary plasmacytoma of bone: a case report.
Wen XU ; Yong YU ; Ya-qin ZHI
Chinese Journal of Hematology 2011;32(12):873-873
Bone Neoplasms
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therapy
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Castleman Disease
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etiology
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Humans
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Male
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Middle Aged
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Plasmacytoma
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therapy
4.Finite element analysis of inferior lumbar spine
zhi-qiang, FEI ; wen-bo, JIANG ; jian-guang, XU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(08):-
Objective To establish a normal lumbar spine 3-dimensional(3D) finite element model(FEM) and discuss the biomechanics of the lumbar motions. Methods A geometrical model was constructed on the basis of a healthy adult male's lumbar CT image.The lumbar spine FEM was established by finite element analysis software.After setting the boundary and loading the burden,the lumbar spine's range of motion and stress distribution in different institutions such as anterior flexion,posterior extension,left or right bending and axial rotating were recorded. Results The lumbar spine FEM included 114 953 tetrahedron units.The results of the FEM matched the results of the in vitro experiment of biomechanics. Conclusion The lumbar spine FEM can imitate the motions of the lumbar spine.
5.Mode of Onset,Infarction Site and Etiological Diagnosis of Arterial Ischemic Stroke in Children
wen-jing, TU ; zhi-sheng, LIU ; san-qing, XU
Journal of Applied Clinical Pediatrics 2006;0(24):-
0.05).The abrupt onset of the inflammatory arteriopathy group was 10 cases(31.2%),the abrupt onset of the noninflammatory arteriopathy group was 38 cases(58.5%),there was significant difference for the mode of onset between the 2 groups(?2=6.352 P
6.Human Umbilical Venous Endothelial Cells as Feeder Layer to Support the Growth of Embryonic Stem Cells
zhi-xu, HE ; zhi-hua, WANG ; qiang, MI ; hao-wen, WANG
Journal of Applied Clinical Pediatrics 2006;0(19):-
Objective To explore whether human umbilical venous endothelial cells could be used as feeder layer to support the growth of embryonic stem cells (ESC) and keep ESC undifferentiated.Methods The venous vessels of umbilical cord obtained from healthy puerperal were perfused with collagenase.The isolated endothelial cells went through primary culture and passages for expansion.Factor Ⅷ antigens determination was implemented.Endothelial cells with good growth and 3 or above passages were treated with mitomycin-C(10 mg/L) and prepared as feeder layer,on which E14.1 ESC was transplanted for subculturing to observe the morphological characterization and determine ESC alkaline psphatase (AKP) activity and the expression of stem cell marker Oct-4.Severe combined immune deficiency(SCID) mouse in vivo terotoma formation experiment was performed to identify its pluripotent properties.Results Human umbilical vein-derived endothelial cells grew well in culturing in vitro and regenerate in large numbers.The endothelial cells maintained normal cellular morphological and biological characterization after 10 passages.The cells stopped proliferating after being treated with mitomycin-C,but its activity and morphological properties were well-maintained with 24 hours,which was a fundamental property of serving as feeder layer.E14.1 ESC remained undifferentiated in human umbilical venous endothelial cells after 3-8 passages,the cells grew in colony and showed high expression of AKP and stem cell Oct-4.In vivo pluripotency experiment showed that 6 weeks after being transplanted to SCID mice E14.1 ESC of 6 and 10 passages in endothelial cells both could form teratoma containing 3 layers of tissue cells.Conclusions Human umbilical venous endothelial cell serve as a convenient feeder layer cell with rich sources.It can effectively support ESC growth and heterogenous and prevent the heterogeneous protein pollution and pathogenic microorganisms caused by animal cell feeder layers,thus solve the problem of biological safety of ESC clinical application.
7.Nasal endoscopic surgery by modified midface degloving approach for spongiform angioma in face: a case report.
Wen-zhong SUN ; Zhi-wen XU ; Ji-hui LI
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2006;41(6):467-467
Adolescent
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Endoscopy
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methods
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Face
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surgery
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Facial Neoplasms
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surgery
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Female
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Hemangioma, Cavernous
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surgery
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Humans
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Nose
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surgery
8.Relationship analysis of urine RBC morphology between UF-100 and phase contrast microscope
Yun-Cheng XIA ; Xu-Guang ZANG ; Zhi-Lan LI ; Xiang-Qing XU ; Wen-Ling JIANG ; LIJIANG
Journal of Chinese Physician 2001;0(09):-
Objective To study the relationship of urine RBC morphology between UF-100 urine sediment analytic instrument andphase contrast microscope.Methods The UF-100 urine sediment analytic instrument to analyze 500 urine specimens and study the relation-ship of urine RBC morphology between urine sediment analytic instrument and phase contrast microscope.Results The according perceptionof Normocytic,Microcytic and Non-classified RBC between phase contrast microscope and UF-100 urine sediment analytic instrument RBC-info are 91.4%,94.4%,83.3% respectively,the according perception between phase contrast microscope and RBC-P70Fsc are 94.9%,95.7%,94.7% respectively,and the according perception between phase contrast microscope and RBC Fsc-DW are 84.4%,86.8%,90.5% respectively,the specificity of UF-100 and phase contrast microscope in glomerular hematuria and non-glomerular hematuria are84.3%,88.1% and 83.3%,87.9% respectively.Conclusion The results show that the UF-100 urine sediment analytic instrument issimply operating,fast and high accurate,and which can instruct clinical dignose,therapy and prognosis judgement.
9.Advance on chemical compounds of Ainsliaea genus.
Fa-jin FENG ; Zhi-ling XU ; Qian-jun ZHANG ; Zhen-hua YIN ; Wen-yi KANG
China Journal of Chinese Materia Medica 2015;40(7):1244-1251
Plants in Ainsliaea genus, belongs to Compositae family, are traditional Chinese medicine and widely used in folk. These plants contain various types of chemical components, and main components are sesquiterpene lactone and its glycosides. In addition, there are triterpenoids, flavonoids, steroids, phenolic acid, long chain fatty acid and volatile oils. Recently, much attention has been payed to varlous research of A. fragrans. This paper reviewed and summarized the chemical components to provide the theoretical basis for the use of Ainsliaea.
Asteraceae
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chemistry
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Molecular Structure
10.Histologic classification of nasopharyngeal carcinoma.
Kuang-rong WEI ; Ying XU ; Wen-jun ZHANG ; Zhi-heng LIANG ; Jing LIU
Chinese Journal of Pathology 2011;40(5):355-357