1.Evaluation of Suprapubic Transvesical Prostatectomy, Madigan Prostatectomy and Transurethral Electrovaporization Ablation Prostatectomy for the Treatments of Symptomatic BPH
Journal of Chinese Physician 2001;0(02):-
Objective To evaluate and compare the therapeutic effects of suprapubic transvesical prostatectomy (SPPC), Madigan prostatectomy (MPC) and transurethral electrovaporization ablation prostatectomy (TUVP) for the treatments of symptomatic BPH. Methods 216 symptomatic BPH patients randomly underwent SPPC(n=87), MPC(n=45) or TUVP(n=84) respectively and their therapeutic effects were evaluated. Results There are significant improvements in symptoms and physical signs in each group after operation. The morbidity rates of post-operative complications in SPPC,MPC and TUVP were 31 0%,11 1%,13 1% respectvely. SPPC group showed the longest average operative time and the most blood loss, both of which were the least in TUVP group. Conclusions SPPC was the most effective among the three procedures, but the trauma of SPPC to the patient was the most. Complication of MPC was the least. TUVP was the safest procedure with the least injury.
2.Formation and identification of virus-like particles of poliovirus type I.
Xiao-Wen WANG ; Wang SHENG ; Yi ZENG
Chinese Journal of Experimental and Clinical Virology 2013;27(5):373-375
OBJECTIVETo establish a method to produce virus-like particles (VLP) of poliovirus type I in Saccharomy cescerevisiae to develop potential novel recombinant vaccine against poliovirus type 1.
METHODSThe genes of P1 and 3CD of poliovirus type I were optimized, synthesized and inserted into expression vector, which was further transfected into Saccharomy cescerevisiae. The extracts of yeast cells were purified by CsCl density gradient centrifugation after induction and cell lysis.
RESULTSElectrophoresis and sequencing analyses showed that the genes P1 and 3CD of poliovirus type I were successfully inserted into expression vector and encode a protein whose amino acid sequences were identical with wide-type genes of poliovirus type I. Electronic microscopy analysis showed that the VLPs of poliovirus type I could be efficiently formed in Saccharomy cescerevisiae.
CONCLUSIONThe VLPs of poliovirus type I could be efficiently produced by co-expression of P1 and 3CD genes in Saccharomy cescerevisiae.
Female ; Gene Expression ; Humans ; Male ; Poliomyelitis ; prevention & control ; virology ; Poliovirus ; genetics ; metabolism ; Poliovirus Vaccines ; genetics ; metabolism ; Saccharomyces cerevisiae ; genetics ; metabolism ; Viral Proteins ; genetics ; metabolism ; Virion ; genetics ; metabolism
3.The Neurobehavioral Cognitive Status Examination(NCSE)
Yan ZHU ; Yi WANG ; Wen-Min WANG ;
Chinese Journal of Clinical Psychology 1993;0(02):-
Objective:This article has summarized the second generation cognitive screening exam(NCSE) about it's subtests,methods of use,applicability,characteristics and flaws.
4.Effects of Betaxolol Hydrochloride Cationic Liposomes on Intraocular Pressure, Blood Pressure and Heart Rate in Rabbits
China Pharmacist 2014;(11):1849-1852
Objective: To study the effects of betaxolol hydrochloride (BH) cationic liposomes on intraocular pressure (IOP), blood pressure ( BP) and heart rate ( HR) in rabbits to lay foundation for the development of new BH eye preparations. Methods:N-trimethyl chitosan (TMC60) was used as the coating material, the BH-loaded cationic liposomes (TMC60-BHL) were prepared. Total-ly 24 healthy rabbits were randomly divided into 4 groups using number method. Unilateral carotid artery intubation was carried out in the rabbits and connected a physiological recorder. TMC60-BHL, uncoated BHL, the marketed BH eye drops ( the positive control) and sterilized distilled water ( the negative control) with the volume of 50 μl was respectively used in one eye of the rabbits. IOP was detected by an ophthalmotonometer, and BP and HR were determined by the physiological recorder at the predetermined time intervals. Results:In the ipsilateral eyes, the maximum decrease in IOP for uncoated BHL and TMC60-BHL was 23. 00% and 26. 65%, respec-tively. In the contralateral eyes, the maximum decrease in IOP for uncoated BHL and TMC60-BHL was 18. 54% and 24. 33%, respec-tively. Compared with that of the positive control (19. 01%,15. 19%), the IOP decrease effect of uncoated BHL and TMC60-BHL was significant higher(P<0. 05), and that of TMC60-BHL was the highest(P<0. 05). BP and HR were both reduced in the various BH groups, while there was no notable difference when compared with those in the negative group(P>0. 05). Conclusion:TMC60-BHL shows significant IOP decrease effect without notable effect on BP and HR, which is valuable to be studied further.
9.Effects of cerium oxide content on the color of alumina-glass composite
Yuanfu YI ; Ning WEN ; Zhongyi WANG
Journal of Practical Stomatology 2001;0(01):-
objective: To investigate the effects of cerium oxide content on the color of alumina glass composite. Methods: Infiltration glass powder with different content of cerium oxide (1%~4%) were fabricated. Alumina glass composite was prepared by infiltrating molten glass into presintered alumina block at 1 250 ℃ for 2 h, and the color of the alumina glass composite were measured with Minolta chromatic instrument(CR 321) . For comparison, the color of VITA In Ceram alumina technical shade guide was recorded too. Results: After infiltration, the b * range of the alumina glass composite was in accord with that of the shade guide. With the increasing content of the cerium oxide, a *, b * and chroma increased, L * decreased, the hue of the alumina glass composite shifted from yellow green to yellow red. Conclusion: Cerium oxide may effectively enhance b * of the alumina glass composite, but its ability of reducing L * and enhancing a * is weak.
10.Color measurements of dental CAD/CAM alumina-galss-composites
Yuanfu YI ; Ning WEN ; Zhongyi WANG
Journal of Practical Stomatology 1996;0(02):-
Objective: To measure the color of dental CAD/CAM alumina-glass-composites(AGC). Methods: Dental CAD/CAM alumina block were infiltrated at 1 120 ℃ by laboratory made AG1,AG2,AG3 and AG4 color serials of infiltration glass powder and Vita In-Ceram Alumina AL1,AL2,AL3 and AL4 infiltration glass respectively. Specimens were made with the area of 10 mm?10 mm,thickness of 0.5,1 and 1.5 mm respectively. Color parameters were measured by Minolta CM-2600d spectrophotometer. Results:The specimen with the thickness of 1 mm was used as the standard for color measurement.After infiltration with AL1-AL4 glass powder,the color parameters of Vita alumina core ceramic were L*: 69.39-78.41,a*:1.82- 4.02,b*:18.35-24.42,when infiltrated with AG1-AG4 glass powder, the color parameters of CAD/CAM AGC were L*:68.80-78.44,a*:1.32- 4.75,b*:16.85- 21.86. b*value and chroma of the AG3 core ceramic were lower than that of AL3 core ceramic,while a* value was higher.b* value and chroma of the AG1 and AG2 core ceramic were lower than that of AL1 and AL2 core ceramic. The reflectance curve of CAD/CAM AGC infiltrated by AG1-AG4 glass powder demonstrated the similar trend as that of the core ceramic infiltrated by Vita In-Ceram glass powder. Conclusion: The color range and surface reflection rate of laboratory made AG1-AG4 AGC are similar with those of Vita AL1-AL4 In-Ceram Alumina core and may meet the need of color match with veneer ceramics.