1.The applied value of 256 spiral CT dynamic volume scanning in the diagnosis of moyamoya disease
Huijuan JIA ; Li WEI ; Yucun FU
Chinese Journal of Postgraduates of Medicine 2014;37(18):12-14
Objective To discuss the value of 256 spiral CT dynamic volume scanning in the diagnosis of moyamoya disease.Methods Twenty-three patients with moyamoya disease undergoing 256 spiral CT dynamic volume scanning (moyamoya group) were selected,and 18 patients having cerebrovascular disease symptoms,but the brain artery without stenosis (control group) were also selected.The volume reconstruction (VR),maximum intensity projection (MIP) and cerebral CT perfusion imaging was displayed.The cerebral CT perfusion imaging parameters of anterior,middle,posterior cerebral artery were measured and analyzed.Results VR,MIP could well reproduce lesion location,degree of stenosis and skull base abnormal vascular network change.Compared with control group,the cerebral blood volume (CBV) was increased [(8.46 ±0.91) ml/100 g vs.(2.92 ±0.72) ml/100 g],time to peak (TTP) was increased [(30.27 ±5.02) s vs.(24.83 ±4.07) s] in anterior cerebral artery,and there was significant difference (P < 0.01),but there was no significant difference in the cerebral blood flow (CBF),the mean transit time (MTT)(P > 0.05).Compared with control group,CBV was increased [(8.06 ± 1.05) ml/100 g vs.(6.08 ± 0.56) ml/100 g],MTT,TTP was increased [(6.34 ± 1.01) s vs.(3.83 ± 0.83) s,(32.06 ± 2.55) s vs.(25.83 ± 2.34) s] in middle cerebral artery,and there was significant difference (P< 0.01),but there was no significant difference in CBF (P > 0.05).Compared with control group,there was no significant difference in the cerebral CT perfusion imaging parameters of posterior cerebral artery (P >0.05).Conclusion 256 spiral CT dynamic volume scanning can be combined with morphology and function imagings,and has important guiding significance for diagnosis of moyamoya disease.
2.The resistance mechanism and response prediction of docetaxel
Li XIE ; Jia WEI ; Baorui LIU
Cancer Research and Clinic 2009;21(6):427-429
Resistance is one of the most important reasons that restrict the clinical application of most chemotherapeutic medicines. Docetaxel is a very widely applicated antitumor medicine. Most of the researches on the mechanism of resistance against docetaxel focused on the drug transporters, changes in drug metabolism and pathway alteration of cell cycle and apoptnsis. The mechanism of docetaxel resistance and the predictive data based on clinical research to docetaxel therapy in cancer treatment were reviewed.
3.Insulin up-regulates expression of apelin gene in 3T3-L1 cells:a preliminary observation
Li WEI ; Xinghua HOU ; Weiping JIA
Chinese Journal of Endocrinology and Metabolism 1986;0(04):-
Expression of apelin mRNA in the isolated mouse adipocytes was confirmed by Northern blot. The level of apelin mRNA increased during stage of differentiation of 3T3-L1 cells. Insulin up-regulated the apelin expression in 3T3-L1 adipocytes, suggesting that apelin expression in adipocyte may correlate with obesity, insulin resistance and hypertension.
4.Updates on genes related to breast cancer metastasis.
Bing-bing LIU ; Jia WEI ; Li FU
Chinese Journal of Pathology 2008;37(4):266-269
Acetyltransferases
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genetics
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Breast Neoplasms
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genetics
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Female
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GPI-Linked Proteins
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Gene Expression Regulation, Neoplastic
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genetics
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Humans
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Membrane Glycoproteins
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genetics
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Neoplasm Metastasis
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genetics
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physiopathology
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S100 Proteins
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genetics
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Transcription Factors
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genetics
5.Effect of CXCL12 in tumor microenvironment.
Fang-Fang LIU ; Jia WEI ; Li FU
Chinese Journal of Pathology 2008;37(3):193-196
6.Phosphorylation of PYK2 and p38MAPK in neurons after focal cerebral ischemia in rats
Xuemei LI ; Junhong JIA ; Wei REN
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
Objective To investigate and evaluate the changes of phospho PYK2 and phospho p38MAPK expression in neurons after focal cerebral ischemia. Methods Focal ischemia reperfusion model was established in rats, and by using immunostaining, the changes of phospho PYK2 and phospho p38MAPK expression in neurons was observed. Results Faint phospho PYK2 and phospho p38MAPK immunoreactivity were revealed in normal cortical neurons. Fifteen minutes after the ischemia onset, a pronounced upregulation of phospho PYK2 and phospho p38MAPK immunoreactivities were evident in these ischemia attacked neurons. The immunoreactivities of phospho PYK2 and phospho p38MAPK reached its peak at 30min after ischemia, and decreased 60min after ischemia. Conclusion Cerebral ischemia was able to induce neuronal PYK2 phosphorylation. The activation of PYK2 might link ischemia attack to the p38MAPK signaling pathway to initiate the neuronal response to the stress stimuli
7.Inhibitory effects of ?-lipoic acid on activation of NF-?B induced by high glucose in rat mesenteric cells
Medical Journal of Chinese People's Liberation Army 2001;0(11):-
Objective To investigate the effects of ?-lipoic acid on the proliferation and activation of NF-?B induced by high glucose (HG) in rat mesenteric cells (MCs). Methods The rat mesenteric cells were cultured in the medium with normal glucose (5.6mmol/L, NG), high glucose (25mmol/L, HG), HG+100 ?mol/L ?-lipoic acid, or HG+200?mol/L ?-lipoic acid and HG+PDTC (a NF-?B inhibitor). Activation of nuclear factor-?B (NF-?B) of rat mesenteric cells were measured by electrophoretic mobility shift assay (EMSA). The cell proliferation was assessed by MTT. Results ?-lipoic acid (50~300?mol/L) can inhibit the proliferation of MCs. The NF-?B binding activity was 2.2 -fold higher in MCs exposed to HG compared to NG (P
8.Detection of Extended-spectrum Beta-lactamases and Analysis of Antibiotics Resistance of Clinical Isolates from Pneumonia Patients
Chinese Journal of Nosocomiology 2006;0(08):-
0.05).Except imipenem and ampicillin,there was significant difference between the resistance of non-ESBLs-producing strains from CAP and from HAP to other eleven kinds of antibiotics(?2 test,P
9.Extended-spectrum ?-Lactamases and Resistance in Escherichia coli Isolated from Biliary Tract and Abdominal Cavity
Yunsong LI ; Jia WEI ; Zizhong XIONG
Chinese Journal of Nosocomiology 2006;0(05):-
OBJECTIVE To investigate the prevalence of extended-spectrum ?-lactamases(ESBLs) and the resistance in Escherichia coli isolates from biliary tract and abdominal cavity.METHODS ESBLs-producers were detected by CLSI Phenotypic Confirmatory Test and susceptibilities were tested by agar dilution method.RESULTS 50.1% Of isolates were ESBLs producers in those isolates.ESBLs producers were highly resistant to ampicillin,cefazolin,cefuroxime,fluoroquinolones,and cefotaxime.The resistant rate to ceftazidime,cefepime,cefoperazone-sulbactam,amikacin,and cefmetazole was less than 40%.None was resistant to meropenem in ESBLs producers.In non-ESBLs producers the resistant rate to ampicillin,cefazolin,cefuroxime,fluoroquinolones was more than 40% and most were susceptible to other antimicrobial agents.The resistant rate to ampicillin,cefazolin,cefuroxime,and ciprofloxacin was significantly higher in ESBLs producers than non-ESBLs producers.CONCLUSIONS With resistance to most of antimicrobial agents,ESBLs-producers were highly prevalent in E.coli isolates from biliary tract and abdominal cavity,so more attention should be paid to survey and detect those strains.
10.Clinical observation of neutrophil adhesion molecules expression during coronary artery surgery under cardiopulmonary bypass
Wei ZHAO ; Chenghui LI ; Naiguang JIA
Chinese Journal of Thoracic and Cardiovascular Surgery 1995;0(05):-
Objective To investigate the changes of CD11b on neutrophils during myocardial ischemia-reperfusion injury. Methods 12 patients undergoing coronary bypass grafts under CPB were studied. Blood samples were obtained from the coronary (coronary sinus) and systemic circulation (central venous and CPB machine), respectively. Samples obtained from coronary and systemic circulation were taken before the beginning of CPB, during CPB and after cardiac reperfusion. Neutrophil CD11b expression and serum cTnI concentration were measured. Results (1)Expression of CD11b increased gradually in systemic circulation during CPB and after reperfusion (P