Bone Neoplasms ; surgery ; Humans ; Pelvic Bones

Arthroplasty, Replacement ; Bone Neoplasms ; drug therapy ; pathology ; surgery ; Bone Transplantation ; Humans

Liquid biopsy plays an important role in the process of precision medical treatment.Its clinical value in the diagnosis and treatment of various diseases has being constantly proved.Colorectal cancer with high incidence begins subtly.The applicaiton of efficient early screening and diagnostic methods can effectively reduce the disease damage,and the implementation of precise post-operative and medication monitoring as the guidance for therapeutic strategy is helpful to improve the prognosis of patients.We should seize the opportunity to promote the transformation of research findings into the clinical application of colorectal cancer.Clinicians,technicians and regulators should face up and work together to overcome the challenges met during the process of clinical liquid biopsy.

Objective To analyze the whole genome sequence and genetic characteristics of the Yunnan isolate of Coxsackievirus A8(CVA8),A8-1/YN/CHN/2019,in order to understand the prevalence and variation of CVA8.Methods The primers for CVA8 were designed,the viral RNA was extracted,the VP1 sequence was amplified by RT-PCR and sequenced,and the whole genome was spliced using BioEdit 7.2.3. MEGA 7.0 software was used for the phylogenetic analysis,and Simplot 3.5.1 and RDP4 software were used for the recombination analysis.Results Strain A8-1/YN/CHN/2019 was7 396 nt long and encoded 2 188 amino acids. The homologies of nucleotide and amino acid sequences between VP1 and CVA8 prototype strain AF081299/Donovan/USA/1998 were 81. 04% and 95. 24%,respectively. Strain A8-1/YN/CHN/2019 belonged to genotype E,while the other Chinese isolates were located in genotypes D and E. Recombination analysis revealed that strain A8-1/YN/CHN/2019 recombined on segments P2 and P3 of the non-structural coding region.Conclusion Strain A8-1/YN/CHN/2019 is of genotype E and has recombination events in the non-structural regions of the P2 and P3 segments.

Researchers have made remarkable progress in developing more advanced assays of liquid biopsy in recent year. More studies have demonstrated the tremendous potential of liquid biopsy biomarkers in changing cancer diagnosis and treatment scenarios. Facing unprecedented opportunities and challenges, scientists must achieve a vital milestone by transforming liquid biopsy from research experimental methods into a molecular diagnostic product with clear clinical utilization.

Liquid chromatography-mass spectrometry (LC-MS) technology is playing an increasingly important role in clinical precision medicine. Laboratory medicine professionals should pay attention to the universal applicability and particularity of LC-MS technology in quality management. The C62-A guideline issued by the Clinical and Laboratory Standards Institute (CLSI) stipulates that LC-MS must meet the need of special 'additional quality assurance' -analytical run setting, which is worthwhile for reference. We need to establish a specific strategy for a set of standardized analytical run which is suitable for medical laboratories in China, aiming to ensure the accuracy of results of LC-MS tests.

目的探讨社会支持网络对截瘫患者的作用。方法采用问卷调查方法调查84例住院截瘫患者,对所得数据进行统计分析。结果在出现困难时,大多数患者首先想到的是求助于单位,选择求助者第1位依次为:单位领导(75%)、自己解决(13.1%)、朋友(5.9%)、律师(4.7%);第2位依次为:亲戚(36.9%)、政府部门(20.2%)、单位领导(5.9%);第3位依次为:法院(28.5%)、朋友(16.7%)、亲戚(10.7%)。结论国家、单位、各类社会团体和组织、家庭以及来自于非正式社会网络的支持应相互配合,建构完整有效的社会支持网,以使个体抵御在社会生活中所遇到的各种风险。