1.Induction of Magnolia officinalis Rehd.et Wils.var.biloba Rehd.et Wils. Callus and Contents of Active Components in Callus from Different Sources
Yeman LIU ; Biqing ZHAO ; Ting ZENG
China Pharmacy 2005;0(18):-
OBJECTIVE: To study the induction and growth of Magnolia officinalis Rehd.et Wils.var.biloba Rehd.et Wils. callus and to compare the content of active components in callus from different sources.METHODS: The factors influencing the induction and growth of Magnolia officinalis Rehd.et Wils.var.biloba Rehd.et Wils. callus were studied by single factor analysis and orthogonal design. The contents of active components in callus from different sources were determined by HPLC. RESULTS: In the various explants, the apical buds and stem fragments exhibited the best ability of dedifferentiation to form callus, followed by pistils. The optimum culture medium of the callus induction was B5+NAA0.1 mg?L-1+6-BA1.0 mg?L-1, while the culture medium B5+NAA0.5 mg?L-1+6-BA4.0 mg?L-1 markedly improved the growth of the callus; after secondary culture of the callus induced by the WPM basic medium, the total content of Magnolol plus Honokiol ranged from 0.167 9% to 0.234 4%, but the induction rate of the callus was low at 33.08%~48.65%. Under the culture condition of MS+NAA0.5 mg?L-1+6-BA1.0 mg?L-1,the rate of callus induction was 62.12% and the total content of Magnolol plus Honokiol after secondary culture was 0.168 6%. CONCLUSION: The results serve as reference in the tissue culture of Magnolia officinalis Rehd.et Wils.var.biloba Rehd.et Wils and the screening of the high production cell line.
2.Children's hearing behavior observations and high risk individual genetic screening for late-onset hearing loss early detection and intervention exploring a basic-level hospitals model.
Yilian GUO ; Xiangli ZENG ; Ting LIU ; Yudi ZOU ; Yanchou YE
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(18):1618-1621
OBJECTIVE:
To explore the methods to detect and intervene children's late-onset hearing loss early which are suitable for basic-level hospitals.
METHOD:
Udiology and imaging diagnosis had been given to the children who passed the newborn hearing screening but showed auditory behavior disorders in the growth process, and individualized interventions were given according to the results of diagnosis. Seven children with high risk for hereditary deafness were sent to superior hospital and had molecular screening of common mutations of inherited deafness carried out, then corresponding prevention guidance and intervention were given to them.
RESULT:
Fifty-two cases with late-onset hearing loss or verbal disorders were detected by auditory behavior observations,including 4 cases of auditory neuropathy, 4 cases of unilateral sensorineural deafness, 27 cases of secretory otitis media. 13 cases of bilateral sensorineural deafness and 4 cases of autism. Seven newborns with high risk of hereditary deafness were sent to the Third Affiliated Hospital of Sun Yat-Sen University and received molecular screening of common mutations of inherited deafness. One case with GJB2 compound heterozygous mutations was detected and followed up to 4 years old, he was found bilateral moderate hearing loss and accepted the hearing aids at 2 years old. Mitochondrial DNA 1555 a > G heterogeneity mutation in 2 cases and GJB2 235 delC single heterozygous mutations in 3 cases, no mutation in 1 case, all these 6 cases have been followed-up until now, their hearing are normal.
CONCLUSION
Children's auditory behavior observations and the superior hospitals referral performing high risk individual screening for newborns with high risk for hereditary deafness can detect children's late-onset hearing loss in time, this model is suitable for basic-level hospitals.
Behavior Observation Techniques
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Child
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Connexin 26
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Connexins
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genetics
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DNA Mutational Analysis
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DNA, Mitochondrial
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genetics
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Deafness
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diagnosis
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genetics
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Early Diagnosis
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Genetic Testing
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Hearing Tests
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Heterozygote
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Humans
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Infant, Newborn
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Male
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Mutation
3.Plasma Concentration of Phencynonate Hydrochloride in Dogs by HPLC-MS/MS and Its Pharmacokinetic Study
Yuan ZENG ; Lulu ZHENG ; Lei XIONG ; Hui LIU ; Ting DUAN
China Pharmacy 2015;(28):3912-3914,3915
OBJECTIVE:To determine the plasma concentration of Phencynonate hydrochloride(PCH)in dogs,and to calcu-late pharmacokinetic parameters. METHODS:6 Beagle dogs were given PCH tablets(2 mg and 4 mg)intragastrically. The blood samples were collected 5 min before medication and 0.17,0.25,0.5,0.75,1.0,1.25,1.5,2,3,4,6,8,10,12,14,16,18, 24 and 36 h after medication,2 ml each time. Using penehyclidine hydrochloride as internal standard,HPLC-MS/MS method was adopted to determine the plasma concentration of PCH. The medication plans were interchanged 2 weeks later. The pharmacokinetic parameters were calculated using DAS 2.0 software. RESULTS:The linear range of PCH was 0.1-15 ng/ml(r=0.999 6);the low-est limit of quantification was 0.1 ng/ml;the methodology recovery were 97.30%-103.20%;the extraction recovery were 52.30%-60.11%(RSD<11%,n=5). The main pharmacokinetic parameters of low and high doses were as follows as t1/2α of (0.678±0.525)and(0.405±0.465)h,tmax of(1.042±0.401)and(0.900±0.418)h,cmax of(14.063±6.29)and(31.580±9.673) mg/L,AUC0-36 h of(48.186±14.776)and(79.269±34.649)mg·h/L. CONCLUSIONS:The method is simple,sensitive and speci-fic,and can be used for pharmacokinetic study of PCH in dogs.
4.Treatment for POEMS syndrome with subcutaneous administration of bortezomib followed by autologous peripheral blood stem cell transplantation: a case report and literatures review.
Ke ZENG ; Zhi-gang LIU ; Jin-rong YANG ; Li HOU ; Yu-ming YANG ; Ting LIU ; Ting NIU
Chinese Journal of Hematology 2013;34(6):550-552
5.Sequence structure and phylogenetic analysis of the chloroplast genomes of Alangium chinense (Lour.) Harms and its different subspecies
Xiao-ying YANG ; Chang LIU ; Xian-fa ZENG ; Xiong-wei LIU ; Jie-hong ZHAO ; Ting-ting FENG ; Ying ZHOU
Acta Pharmaceutica Sinica 2022;57(10):3229-3239
italic>Alangium chinense is a commonly used medicinal plant of Alangiaceae
6.Clinicopathologic analysis of adrenocortical diseases
Changxin JIANG ; Zhi ZENG ; Ting WANG ; Xin LIU ; Rong LIU ; Ying LI
Chinese Journal of Urology 2012;33(3):165-170
ObjectiveTo investigate the correlationship between clinical and histopathological results of adrenocortical hyperplasia,adenoma and adenocarcinoma in the adrenocortical glomerular,fascicular and reticular zones. MethodsA total number of 167 702 surgical specimens were collected by the Department of pathology at the General Hospital of Tianjin Medical University from 1993 -2008.Of these,there were 631 adrenocortical specimens.Statistic analysis was done with Run test,t or t'test and x2 test on data in the aspects of proportion rate,mean age,sex proportion and relationship between clinical and pathological results of adrenocortical diseases.ResultsThe total number of adrenal diseases and adrenocortical diseases were 910 cases (0.54%) and 631 cases (0.38%),respectively.The number of adrenocortical glomerular,fascicular,reticular diseases were 310 cases (49.13%),319 cases (50.55%) and 2 cases (0.32%),respectively.The rate of adrenal diseases and adrenocortical diseases specimens did not change during the 16 year study period.The trend in proportion of hyperplasia,adenoma,adenocarcinoma in adrenocortical glomerular,fascicular and reticular zones did not change as well.The proportion pattern showed that there were more cases of adenoma than of hyperplasia and adenocarcinoma.The functional diseases of adrenocortical glomerular,fascicular and reticular zone (90.97%,79.00%,100.00% ) were higher than nonfunctional diseases in the corresponding zones (9.03%,21.00%,0% ) and both were higher in female patients.The mean age of nonfunctional patients with adenoma,glomerular zone adenocarcinoma and hyperplasia,adenoma of fascicular zone was higher than that in functional diseases.Interestingly,the mean age of glomerular zone adenocarcinoma patients (62.7 yrs) was higher than that in hyperplasia patients (47.8 yrs) and in adenoma patients (44.8 yrs).The mean and male diagnostic ages of adenocarcinoma of fascicular (44.3 yrs,50.0 yra) were higher than that in adenoma (44.0 yrs,49.1 yrs) and hyperplasia (41.5 yrs,40.9 yrs) respectively.But the female mean diagnostic age of fascicular adenocarcinoma (38.7 yrs) was lower than that in adenoma (42.4 yrs) and hyperplasia (41.9 yrs) respectively and the lowest average age was for nonfunctional adenocarcinoma (34.4 yrs).The diameters of the functional and nonfunctional adenocarcinoma were large (4.3 cm and 4.0 cm in glomerular zone,7.1 cm and 8.0 cm in fascicular zone).The carcinoma cells were in serious atyrpia,haemorrhage,necrosis and capsular or vascular infiltration.The rates of functional hyperplasia,adenoma with hypertension in Conn,Cushing or adrenogenital syndrome of adrenocortical glomerular,fascicular,reticular zone were higher than that without hypertension,but their tumor diameters were similar.The diameters of functional adenoma in glomerular or fascicularer ( 1.7 cm,2.3 cm ) were lower than that of nonfunctional adenoma (2.3 cm,2.4 cm ) and the diameters of glomerular tumors were smaller than that of fascicular tumors.Nonfunctional adenoma ( 2.5 cm,2.0 cm ) of glomerular or fascicular by check up finding were bigger than that of non-endocrine adenoma ( 2.5 cm,2.3cm).There were seven cases with adrenogenital syndrome (2 male cases,5 female cases ),among these,there were 4 fascicularer adenoma cases,1 adenocarcinoma case,1 network hyperplasia case and 1 adenoma case.ConclusionsGenetic abnormality plays an important role in pathogenesis of adrenocortical disease and environment had less influence.Radiological examinations (CT and MRI) of adrenocortical diseases are the first choice and have important roles in evaluating tumor characteristics.There is close relationship between clinical manifestation and pathology.However,the exact diagnosis of definite type of disease and nature of tumor needs to be made by pathologic examination.
7.Determination of plasma concentration of quercetin, kaempferid and isorhamnetin in Hippophae rhamnoides extract by HPLC-MS/MS and pharmacokinetics in rats.
Yu LIU ; Juan YANG ; Yang-ling TUO ; Ting WEI ; Yong ZENG ; Ping WANG ; Xian-li MENG
China Journal of Chinese Materia Medica 2015;40(19):3859-3865
To establish an HPLC-MS/MS method for the analysis of quercetin, kaempferid and isorhamnetin in rats plasma and study its pharmamacokinetics after an intragastrical administration of Hippophae rhamnoides extracts. Five healthy male Sprague-Dawley (SD) rats were given single doses of H. rhamnoides extracts (quercetin 26.35 mg x kg(-1), kaempferid 4.040 mg x kg(-1), isorhamnetin 31.37 mg x kg(-1)), and then their orbital sinus blood samples were collected at different time points. The drug plasma concentration of the three flavonoids was determined by HPLC-MS/MS method. After that, the main pharmacokinetics parameters were calculated by using Kinetica 5. 0. 11 software. The methodological test showed that the linear concentration ranges of quercetin, kaempferid and isorhamnetin were 7.500-600.0 μg x L(-1) (R2 = 0.998 5), 1.000-80.00 μg x L(-1) (R2 = 0.998 5 ) and 10.00-800.0 μg x L(-1) (R2 = 0.998 0), respectively. The inner and inter-days precisions were both less than 14.0%. The plasma samples showed a good stability and consistency with the requirement of biological sample analysis after the samples were frozen once and placed at - 20 degrees C for 15 d and room temperature for 6 h and the treated analytes were placed at -20 degrees C for 24 h. For quercetin, the pharmacokinetic parameter t(½β), AUC(0-∞), MRT(0.∞), C.(max) and T(max) were (113.3 ± 19.37) min, (12 542.14 ± 3 504.05) μg x h x L(-1), (119.6 ± 13.29) h, (164.6 ± 27.33) μg x L(-1) and (5.199 ± 0.840 3) h, respectively. For kaempferid, the pharmacokinetic parameters t(½β), AUC(0-t), MRT(0-∞), C(max) and T(max) were (79.85 ± 17.15) min, (934.51 ± 94.59) μg x h x L(-1), (81.50 ± 13.75) h, (80.15 ± 14.24) μg x L(-1) and (3.827 ± 0.902 7) h, respectively. For isorhamnetin, the pharmacokinetic parameters t1,2,, AUC(0-t), MRT(0-∞), C(max) and T(max) were (118.3 ± 20.73) min, (26 067.77 ± 4 124.60) μg x h x L(-1), (129.0 ± 16.30) h, (269.6 ± 29.32) μg x L(-1) and (6.513 ± 1.450) h, respectively. The HPLC-MS/MS analysis method established in this study was proved to be sensitive and accurate and could be applied in the pharmacokinetic study of quercetin, kaempferid and isorhamnetin in rat plasma.
Animals
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Chromatography, High Pressure Liquid
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methods
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Drugs, Chinese Herbal
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analysis
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pharmacokinetics
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Hippophae
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chemistry
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Kaempferols
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blood
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pharmacokinetics
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Male
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Quercetin
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analogs & derivatives
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blood
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pharmacokinetics
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Rats
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Rats, Sprague-Dawley
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Tandem Mass Spectrometry
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methods
8.Relationship between illness perceptions and health behavior of patients with lower extremity arterial disease
Dongyan ZENG ; Ting HU ; Xiyin LIN ; Xiuqing BU ; Shuwen WU ; Jinling LIU ; Weiming ZHOU ; Shaomang LIN
Modern Clinical Nursing 2016;15(3):16-20
Objectives To explore the relationship between illness perceptions and health behavior in the subjects. Methods Revised illness perception questionnaire and health promoting lifestyle profileⅡ (HPLPⅡ) were used to measure 101 patients′illness perception and health behavior between the groups. Results The HPLPⅡscore of LEAD was(2.17 ± 0.29) with the score of dimension. The course of disease, prognosis and perception of emotion were negatively related to health behaviour; personal control and primaty congtion of drsease were postively related to health behaviour (all P<0.01). Conclusion Medical personnel should pay attention to the relationship between illness perceptions and health behavior in patients with LEAD and effective measures should be taken to improve the patients′illness perceptions, promote their choices and persistence in health behavior and improve the quality of life.
9.Oriented immobilization of papain on metal chelating carriers.
Lin-Lin LIU ; Li-Xi ZENG ; Ting LIU ; Deng LE
Chinese Journal of Biotechnology 2005;21(5):789-793
Based on the technology of protein separation with immobilized metal ion affinity chromatography (IMAC), a method for oriented immobilization of papain has been selected. Papain was successfully immobilized on magnetic agarose carriers. Cu2+ with iminodiacetate (IDA) was used as the chelating ligand to be correlated with the histidine on papain (His-81). The optimum immobilization conditions of enzyme were as flows: Cu2+ 0.15 x 10(-2) mol/g carriers, time was 4h, enzyme load was 30 mg/g carrier, pH was 7.0, respectively. The pH and temperature were 8.0 and 70 degrees C for immobilized enzyme. The recovery activity of immobilized enzyme was retained 68.4%. The carrier could be recovered from the spent immobilized enzyme, to be reused. After 5 times, the reimmobilization of papain on the regenerated matrix was 79.71% effective with the retention of maximum enzyme activity. The cost of carriers used for industrial applications is very important. The regenerability of carriers is therefore, relevant. The mild conditions used for immobilization, the high recovery of immobilized preparations, the stability and the regenerability of the matrix are the main features of the method reported here. All above indicate this method can be applicable and promising in enzyme immobilization field.
Catalysis
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Chelating Agents
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chemistry
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Chromatography, Affinity
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methods
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Copper
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chemistry
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Enzyme Stability
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Enzymes, Immobilized
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chemistry
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Hydrogen-Ion Concentration
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Imino Acids
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chemistry
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Metals
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Papain
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chemistry
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Temperature
10.Effect of health education on control of diabetes mellitus and prevention of its complications
Sai-chun ZHANG ; Yan-ni JIANG ; Jing AN ; Hui-li LIU ; Hong-zhen LIU ; Ting ZENG ; Hai-bin ZHOU
Chinese Journal of Practical Nursing 2011;27(28):51-52
Objective To discuss the active role of health education on control of diabetes mdlitus and prevention of its complications.Methods 96 cases of hospitalized patients with diabetes mellitus were randomly divided into the control group and the observation group with 48 patients in each group from June 2009 to June 2010.The control group received conventional diabetes treatment and distribution of health education brochures,based on this,the observation group was given health education,including cognitive,nutrition,behavioral intervention.The general prevention condition of diabetes and its complications as well as the education effect were compared before the education and six months after education.Results The fasting blood glucose,2-hour postprandial blood glucose,glycosylated hemoglobin,body mass index and incidence of complications were greatly improved in the observation group than those of the control group.Conclusions Strengthening health education can improve self-care ability of patients,effectively improve the overall control level of diabetes,reduce acute and chronic complications,disability,death rate.