PTEN gene is considered to be the first discovered tumor suppressor gene with dual specificity phosphatase activity.The research has shown that the increasing popularity of PTEN rests on its important roles in cancer cell growth,differentiation,signaling transduction and cell apoptosis.Besides,the mutation of PTEN is related to cancer initiation and progression.The recent advances on PTEN gene in malignant tumors will be reviewed in this paper.

Type 2 diabetic patients are usually accompanied by dyslipidemia.The cardiovascular residual risk is still high in these patients,even with glycemia,blood pressure,and plasma lipids well controlled.In this review,the relationship of plasma lipids and changes in lipoprotein particles with cardiovascular risk is discussed.

Objective To explore relationship between magnetic resonance imaging (MRI) performance and lipid levels in pa-tients with lacunar infarction (LI) .Methods 68 patients with LI were retrospectively analyzed ,62 cases patients without heart ce-rebrovascular disease in the same period were choosen as control group ,the multivariate logistic regression was used to analyze the relationship between MRI performance ,the changed level of lipid .Results A total of 299 lacunar lesions were detected by MRI ,in which there were 58(19 .40% )in the left cerebral hemisphere ,26(8 .70% ) in the right hemisphere ,73(24 .41% ) in the bilateral basal ganglia ,27 (9 .03% ) in thalamus ,54 (18 .06% ) in the lateral ventricles corona radiata ,39 (13 .04% ) in brainstem ,and 22 (7 .36% ) in cerebellum .TC ,TG ,LDL-C and Ox-LDL levels in LI patients were significantly higher than the controls (P<0 .05) , while HDL-C levels were significantly lower than the controls (P<0 .05) .Multivariate Logistic regression analysis showed that relative risk of TC ,TG ,LDL-C ,HDL-C and Ox-LDL to LI were 1 .56 ,1 .27 ,1 .39 ,1 .46 and 1 .44 .Conclusion The MRI manifestations and lipid levels of LI have a certain degree of correlation ,and elevated blood lipid levels may be important risk factors for LI .

Objective:To study the variation of X chromosome centromeric Alpha Satellite DNA in Klinefelter's syndrome patients,their parents and normal individuals and to discuss the mechanism of Klinefelter's syndrome X chromosome nondisjunction.Methods:The multi-copies of a 2Kb tandem repeat unit on X chromosome of centromeric Alpha Satellite DNA were simultaneously amplified with reasonable primers by (representative sampling of multiple repetitive units,rep)PCR,and the mechanism of X chromosome aneuploidy resulted by abnormal structure of centromere was investigated.Results:1774bp,570bp and 1410bp,583bp,220 bp (three short fragments are resulted by deletion in alpha satellite DNA) were detected in Klinefelter's syndrome patients,their parents and normal karyotype controls.There were 1774bp and 1410bp DNA fragments in all of samples.The relative frequencies of 570bp and 583 bp were significantly higher in Klinefelter's syndrome patients than in normal karyotype controls,the 220bp was not found significantly different in the three groups.Conclusion:Deletion in the region of Alpha Satellite DNA is present at centromeres of all human X chromosome,while Klinefelter's syndrome patients and their parents show higher frequency than normal individuals.These results imply that analysis of deletion on X-specific ?satellite of the parents during the pre-pregnancy and pregnancy plays a important role either in estimating the risk of repruducing Klinefelter's syndrome or in providing the guideline for prenatal chromosome diagnosis of villus and amniotic fluid.

The roots ofApium graveolens L. have been widely used in Uighur Medicine. Research has shown that roots ofApium graveolens L. has good clinical efficacy in the aspects of liver protection and lipid decreasing, hyperlipidemia treatment and so on. This article reviewed research on the chemical components, extraction process, and pharmacological action of roots ofApium graveolens L. so far, with purposes to better develop and utilize roots of Apium graveolens L., and provide references for follow-up study.

Due to the influence of potential psychological trauma, athletes in the competition may repetitively suffer poor performances, such as losing his usual form, slump, choking, and so on. These under-performing problems actually are symptoms of sports post-traumatic stress disorder (SPTSD). SPTSD refers to the pathological physical and mental after-effects of a trauma previously suffered by the athlete on that athlete's subsequent performances. This is a very common physical and emotional problem in athletics that impairs athletes' performance, affecting their competition results. By peak performance eye movement desensitization and reprocessing therapy, therapists can deal with this problem effectively and help athletes eliminate symptoms of sports post-traumatic stress disorder, improving the competition performance.

Objective To investigate the influence of genistein(Gen) on the growth and apoptosis regulation of hepatoma carcinoma cells SMMC-7721.Methods SMMC-7721 cells were divided into three Gen groups treated with different concentrations(5.0,10.0,20.0 mg?L-1) and control group(without Gen).After SMMC-7721 cells were treated for 24 and 48 h,the changes of ultrastructure of cells were observed under electron microscope, MTT was used to detect the inhibitory rate of proliferation of SMMC-7721 cells,flow cytometry was performed to analyze cell phase;immunohistochemistry was used to detect the expression of Caspases-3 protein.Results Compared with control group,chromatin in cellular nucleus became conglomeration or lumping,with chondriosome swelling in Gen groups.With the increasing of Gen concentration,the cellular nucleus became condensed,heterochromatin agglutinating,ground cytoplasm obviously cavitating;with the increase of Gen concentration and prolongation of time,from 24 to 48 h,MTT showed the inhibitory rate of proliferation of SMMC-7721 cells increased in a time-and dose-dependent manner.Flow cytometry analysis showed that with the increase of Gen concentration,the number of cells at G2/M phase increased,the number of cells at S phase decreased and the ratio of G0/G1 also decreased.Immunohistochemistry results showed the expression of protein Caspases-3 increased with the Gen concentration in a dose-dependent manner(P

Objective To explore the preliminary practice of Clinical Scenario Mode in the teaching of surgery. Methods Clinical Scenario Mode was stimulated or rebuilt by the cooperation of teachers and Standardized Patients (SP) to introduce relative theories and raise discussions in the surgery lectures of five-year clinical medicine students in grade 2004 and grade 2005. Results Lectures with the application of Clinical Scenario Mode were reported positively by students from the survey. It improved students’learning motivation,enhanced teaching quality,and optimized the allocation of resources. Discussion With the combination of the advantages of lecture based learning (LBL) and problem based learning (PBL),Clinical Scenario Mode can be considered as the linkage to the widespread use of PBL,and should be widely applied in the medical teaching practice.

Objective To explore the effect of bcl 2 in vascular smooth muscle cells(VSMC) apoptosis induced by nitric oxide(NO),we investigated the effect of NO on Bcl 2 protein expression in cultured vascular smooth muscle cells(VSMCs),and effect of Bcl 2 over expression on apoptosis is(VSMCs) induced by NO. Methods Cultured rat VSMCs at passage 5 to 8 were used for experiments,S nitroso N acetypenicillamine(SNAP) was used as NO donor.Cells were preincubated for 24-48 hours in special culture dish with conditioned medium to reach quiescent,and then incubated in conditioned medium containing 0 5 mmol/L SNAP.After 8 to 10 hours,the cells were fixed and Bcl 2 expression was analyzed by ACAS 570 through fluorescent immunochemistry.Vascular smooth muscle cells were transfected with bcl 2 gene through retrovirus to allow overexpression of Bcl 2 in VSMCs.Finally,we observed the apoptosis in normal VSMCs and VSMCs infected with bcl 2. Results SNAP downregulated Bcl 2 expression in VSMCs.PCR and Southern hybridization confirmed the integration of the bcl 2 retrovirus vector into vascular smooth muscle cells genomic DNA.And fluorescence immunohistochemistry confirmed that Bcl 2 protein was overexpressed in VSMCs infected with bcl 2.And ratio of apoptosis become lower in VSMCs infected with bcl 2. Conclusion bcl 2 can inhibit apoptosis of VSMCs induced by SNAP.It is suggested that downregulation of Bcl 2 protein is one of the ways in which SNAP induces VSMCs apoptosis,and apoptosis in VSMCs induced by SNAP is regulated by bcl 2.;

Objective: A reversed phase HPLC method was described for determination of prim O glucosylcimifugin and 4′ O ? D glucosyl 5 O methylvisamminol in Ganmaoqingre Granules. Methods:The sample was separated on ODS column with mobile phase of methanol 40 mmol?L -1 sodium acetate pH 6.9 (35∶65) for prim O glucosylcimifugin and H 2O methanol THF(62∶38∶1) for 4′ O ? glucosyl 5 O methylvisamminol. The flow rate was 0.8 mL?min -1 , and the detection was set at 254 nm. Results: The calibration curves were linear in the range of 0.72 ?g?mL -1 ～6.5?g?mL -1 for prim O glucosylcimifugin and 0.92?g?mL -1 ～16.5?g?mL -1 for 4′ O ? D glucosyl 5 O methylvisamminol( r =0.9999). The average recovery was 100.3% and 94.7%. The content of prim O glucosylcimifugin and 4′ O ? D glucosyl 5 O methylvisamminol in Ganmaoqingre Granules was 0.133 mg?g -1 and 0.167 mg?g -1 , respectively. Conclusion: The method is fast and specific for both constitutents of Ganmaoqingre Granules.