Objective To investigate the influences of carbon dioxide (CO2) pneumoperitoneum and operation position on intraocular pressure (IOP) during laparoscopic surgery under general anesthesia. Methods Thirty-six patients were divided into two groups of udergoing laparoscopic pelvic surgeries (group A) and udergoing laparoscopic cholecytectomies(group B) with 18 cases each. The patients in group A were operated under 25 degrees head-down position and those in group B under 25 degrees head-up position. IOP was recorded before surgery(T0), at 5 min after tracheal intubation (T1), 5 min after CO2 pneumoperitoneum(T2), 5 min(T3) and 30 min(T4) after body position changed and the time of normal PETCO2 regained(T5). Results IOP was remarkably decreased after induction of general anesthesia in both groups. IOP of group A raised to (21.0±1.6) mm Hg at T4, which was dropped to (14. 6±1.6) mm Hg at T5. IOP of group B raised to (12.1±2.9) mm Hg at T4,which was dropped to (11.1±1.2) mm Hg at T5. Changes of IOP of both groups were positively correlated with the changes of PETCO2. Conclusion IOP can be decreased remarkably after induction of general anesthesia with propofol. The head-up position and normal range of PETCO2 are the two important factors to avoid an increase of IOP in surgical patients under general anesthesia.

Objective:To study the difference of upper airway form and hyoid position of the adults with different vertical facial types.Methods:Cephalograms of 120 adults with different vertical facial types were retrospectively investigated by computerized cephalometric analysis.Upper airway depths and hyoid position were measured,and differences were compared between male and female,also compared among different vertical facial type groups.Results:The sagittal depth of upper airway in male was larger than that in female,but there was no statistical difference except V-LPW.Among different vertical facial types,the sagittal depths of upper airway in high angle group was smaller than that in average angle group and low angle group.There was statistical difference in hyoid position between genders,AH-SN,AH-FH and AH-C3 in male were larger than in female(P

According to Traditional Chinese Medicine, the functional structure of human body is composed of five functional subsystems which are based on the five-organs concept, and the relationship between them could be expatiated with Wu-Xing (Five-Elements) theory. Wu-Xing system is taken as our research object. The dynamic model has the characteristic of self-organization. There is only one stable point with local stability in the five-dimensional space. There exists an instability domain in the five-dimensional space. The stability domain is unbounded; it contains some special subdomains, including rectilineal subdomains, ray subdomains and line subdomains. In some ray subdomains, there exists comeback summits, the peak value of the comeback summit is comeback threshold. The existence of the comeback threshold means that, if the human body wants to go back to the healthy state from a certain special state which corresponded to the initial conditions located in a domain with comeback summit, it has to get across the comeback threshold. The comeback threshold is the division of the healthy state and the sub-healthy state. Some research work is done to investigate the response of the Wu-Xing dynamic model to the impact stimulation, and results in the decrease of the system's comeback threshold. There are 3 manners to achieve this goal, and they tally with the principle of the Wu-Xing system and the experimental rules in clinic.

Humans ; Liver, Artificial ; Translational Medical Research

The segmental distribution of the afferent fibers traversing the stellate ganglia was studied with HRP techinque in 33 cats. The animals were divided into two groups. In one group HRP was injected into the stellate ganglion of one side. Labelled cells were found in ipsilateral spinal ganglia from C_4 to T_9. The majority of these cells (84.49％) were distribued in T_1 to T_5 segments. There is no conspicuous difference between the left and right in respect to the segmental and the pattern distribution of labelled cells. The majority of the labelled cells were medium or small sized (

OBJECTIVETo evaluate the clinical effects of the massage method of micro-regulating with vertical cross pressing lying on one side in treating cervicogenic headache (CEH).

METHODSTotal 136 patients with CEH were collected in the study from August 2012 to April 2014. They were divided randomly into two groups according to random digits table. Sixty-nine patients accepted the treatment of micro-regulating with vertical cross pressing lying on one side (pressing micro-regulating group), including 29 males and 40 females with an average age of (50.55 ± 11.38) years old; 67 patients received the treatment of traditional massage (traditional massage group), including 28 males and 39 females with an average age of (51.20 ± 11.90) years old. Clinical effect was observed according to the standard of curative effect of State Administration of Traditional Chinese Medicine; the function of cervical vertebra and all body status were evaluated according to NDI score. VAS score, frequency and time of headache were recorded and compared before and after treatment.

RESULTSNo adverse reactions were found after treatment, all patients were followed up from 1 to 6 months with an average of 3.1 months. In pressing micro-regulating group, 25 cases got fully recover, 26 excellence, 14 effectiveness and 4 inefficiency; and in traditional massage group, the results were 12,21,22, 12;clinical effect of pressing micro-regulating group was better than that of traditional massage group (P<0.01). NDI score in pressing micro-regulating group decreased from preoperative 13.48 ± 4.83 to postoperative 6.23 ± 3.76; in traditional massage group also decreased from preoperative 13.82 ± 5.78 to postoperative 8.25 ± 4.75; the improvement of the pressing micro-regulating group was obviously better than that of traditional massage group (P < 0.01). VAS score in pressing micro-regulating group decreased from preoperative 4.75 ± 0.97 to postoperative 1.88 ± 1.78; and in traditional massage group decreased from pre-operative 4.78 ± 0.98 to postoperative 2.84 ± 1.94; pressing micro-regulating group was more notable than that of traditional massage group (P < 0.01). The frequency per week,the pain time in pressing micro-regulating group decreased from preoperative (5.38 ± 1.96) times and (6.87 ± 3.67) hours to postoperative (1.71 ± 2.04) times and (0.97 ± 1.74) hours,respectively,in traditional massage group the above parameters decreased from preoperative (5.22 ± 1.81) times and (6.90 ± 3.79) hours to postoperative (2.81 ± 2.42) times and (1.83 ± 2.21) hours;pressing micr-regulating group was more notable than that of traditional massage group (P < 0.01 or P < 0.05).

CONCLUSIONUsing the tuina method of micro-regulating with vertical cross pressing lying on one side to treat CEH can improve function of cervical vertebra and all body status, lessen the intensity, frequency, duration time of pain, and had advantage of higher security, simple operation, and evident effect.

Adult ; Aged ; Female ; Humans ; Male ; Massage ; methods ; Middle Aged ; Physical Therapy Modalities ; Post-Traumatic Headache ; therapy

Objective To investigate the interaction between HBx protein and ?-catenin for exploring molecular mechanism in HBV-associated hepatocellular carcinoma.Methods Iimmunocytochemistry and Luciferase Assay were used to detect the endocellular location and transcriptional activity of ?-catenin in normal liver cell line L02 infected by Ad-HBx.Results Immunocytochemistry: there was markedly increased expression of ?-catenin in cytoplasm and nucleus.Luciferase Assay: Ad-HBx led to a significant increase of TCF-4-dependent transcription,the TOP count of Ad-GFP and Ad-HBx-GFP was 1.4 fold and 11 fold as compared to the negative control respectively(P

Objective To investigate whether lamivudine antiviral treatment after radical surgery can prolong disease-free survival of patients with hepatitis B virus-related liver cancer.Methods A total of 120 patients with hepatitis B virus-related liver cancer who underwent conventional radical surgery in Dongfeng Hospital Affiliated to Hubei Medical College from March 2014 to March 2016 were enrolled,and among these patients,60 were given conventional treatment (group A) and 60 were given lamivudine antiviral treatment (group B).ELISA was used to measure serum HBV DNA level.The t-test was used for comparison of continuous data between groups,the chi-square test was used for comparison of categorical data between groups,the Kaplan-Meier method was used to compare disease-free survival rate and recurrence rate between groups,and the Spearman method was used to investigate the correlation between serum HBV DNA level and survival time.Results According to the results of the 3-year follow-up,71 patients (59.17％) died (group A:46 patients died of tumor and 4 died of hepatic encephalopathy;group B:20 died of tumor and 1 died of hepatic encephalopathy).Compared with group A,group B had significantly lower recurrence rate (48.33％ vs 90.00％,x2 =16.98,P ＜0.001) and mortality rate within 3 years (35.00％ vs 83.33％,x2 =10.34,P ＜ 0.001).Group B had significantly higher mean disease-free survival time and 1-,2-,and 3-year dis-ease-free survival rates than group A(t =9.82,x2 =7.87,11.43,and 7.98,all P ＜ 0.001).After surgery,group B had a significantly lower serum HBV DNA load than group A [(0.008 1 ±0.003 2) × 105 copies/ml vs (0.014 3 ±0.008 9) × 105 copies/ml,t =18.54,P ＜ 0.001].In group A,there were significant differences in 1-,2-,and 3-year disease-free survival rates between patients with serum HBV DNA load ≥ 1.0 × 105 copies/ml and those with serum HBV DNA load ＜ 1.0 × 105 copies/ml (x2 =8.57,P ＜ 0.05),and further analysis showed that there were significant differences in 1-,2-,and 3-year disease-free survival rates (x2 =4.36,5.36,and 9.53,P ＜ 0.05);in group B,there were no significant differences in 1-,2-,and 3-year disease-free survival rates between these two groups of patients (P ＞ 0.05).Serum HBV DNA expression was positively correlated with patients' survival time (r =0.67,P ＜ 0.001).Conclusion Lamivudine antiviral therapy after radical surgery can effectively reduce serum HBV DNA level,and helps to prolong the disease-free survival time of patients with hepatitis B virus-related liver cancer.

Animals ; Atherosclerosis ; prevention & control ; Biflavonoids ; pharmacology ; Catechin ; pharmacology ; Grape Seed Extract ; pharmacology ; Humans ; Proanthocyanidins ; pharmacology

Objective To investigate the expression of caspase-3 in different post-traumatic intervals and severity of traumatic brain injury (TBI). Method Brain tissue samples of slight (n =24), moderate (n = 24) brain injury and control (n = 10) groups of rat were examined by immunohistochemical staining. Results Up-regulation of caspase-3 expression was found in tissues from traumatic brain injury as compared with controls in 1h after TBI, and lasted for 2 weeks. In tissues taken Ih, 12h, 24h, 72h, lweek and 2weeks after TBI there were different amount of positive celis in brain injuries of different severity. Con-clusion The expression of caspase-3 is different in brain injuries of different severity . These findings are valuable in appraising the severity of injuries.