The full name of the polycystic liver disease is autosomald ominant polycystic liver disease.Surgical treatment is the main method to deal with it at present.With the deep study of the polycystic liver disease into the molecular genetic level,it is possible for molecular diagnostics to achieve presymptomatic and prenatal diagnosis.The article mainly introduce the research progress of the polycystic liver disease's etiology,pathogenesis,diagnosis,treatment,and so on.

Objective: To explore the expression and gene amplification status of human epidermal growth factor receptor-2 (HER2) in the different stages of invasive urothelial bladder carcinoma. Methods:Tumor tissues from 49 patients with different stages of invasive urothelial bladder carcinoma were tested by immunohistochemical staining for HER2 and HER2 gene fluorescence in situ hybridization. Results:The number of male patients was higher than that of females. The positive rate of HER2 protein expression was higher in the patients with the higher stage of invasive urothelial bladder carcinoma. However, no gene amplification was observed in all patients. Twelve patients had ployploid chromosome 17. More ployploids were observed in the patients with the higher stage of inva-sive urothelial bladder carcinoma. Conclusion:The increase in the protein expression of HER2 in the invasive urothelial bladder carci-noma patients was not caused by gene amplification. Other transcriptional and post-transcriptional mechanisms were probably involved in the regulation of the HER2 protein.

Objective To study CT and MRI features of intracranial chondrosarcomas. Methods CT and MRI of 3 cases with intracranial chondros arcomas proved by pathology were retrospectively analyzed.Results CT scans showed the tumors were lobular mass, per itumoral edema were not serious, intratumorous calcification and bone invasion w ere seen in 2 cases.MRI showed that tumor had low to intermediate signal intensi ty or hypointensity on T 1WI, high signal intensity on T 2WI. On contrast enha nced MRI,the center of lesions which was low signal intensity on T 2WI was no e nhancement, but there was observable enhancement at periphery of tumors.Conclusion Intracranical chondrosarcomas are often orgina ted from synchondrosis of the skull base,the lesions are often associated with c alcification, and bone invasion, the accurate dignosis should depend on microsco pic examination and immunohistochemical staining.

Objective To analyse imaging features of atypical meningioma.Methods There were 17 cases with atypical meningiomas proved by pathology,7 cases were male,10 cases were female,ranged in age from 34～69 years.17 cases all examined with CT,and 10 cases with CT enhanced scanning;12 cases examined with MRI and 8 cases with MR enhanced scanning,5 cases examined with DSA.Results The major imaging features of atypical meningioma included:the borders of tumors were mostly irregular,nonhomogeneous density or signal on plain CT and MRI,nonhomogeneous enhancement on enhanced CT and MRI.Cystic and necrotic changing and the invasion of cranial bone and adjacent structure could be seen.5 cases with dural tail sign on MRI,3 of them,this sign was short,thick and irregular.Heavy staining was showed on DSA.Conclusion Atypical meningiomas are of some clinical and imaging features.

Objective To study the clinical law and imaging characteristics of intracranial primary malignant lymphoma.Methods The clinical law and imaging finding in 27 cases of intracranial primary malignant lymphoma confirmed by pathology were analysed CT scan was performed in all cases.MRI examination were carried out in 20 cases simultaneously.Results The first symptom in most cases was headache,and other symptoms were epileopsy,dizziness,hemiplegia,etc.This lesion was mostly seen in male around 40 years old.The lesions were mostly equal or high density on CT,equal or low signal intensity on T 1WI and closed to grey matter signal intensity on T 2WI.Tumors were enhanced obviously on contrast-enhanced scans,necrosis of center of focus was seen in 1/3 cases.The lesions were single or multiple.Conclusion The accuray diagnosis of intracranial primary malignant lymphoma can be done by combined clinical data with imaging features.

Objective To study the correlation among pathological,clinical and the imaging features(CT and MRI) of the Rathke cleft cysts.Methods CT,MRI and clinical findings of Rathke cleft cysts in 43 patients confirmed by operation and pathology were retrospectively studied.Results 27 cysts located at intrasella and suprasella and 16 cysts entirely located at intrasella.Cysts were round or oval in shape with definite borders.The size of 30 cysts exceed 10mm in diameter.On CT scans reviewed,the cysts were low density in 9 cases,hyper-density in 9 cases and isodensity or mixed density in 8 cases.The cyst's wall with calcification was seen in one.On postcontrast CT scans,6 cases showed circular and peripheral cyst's wall enhancement and others were no enhancement.On MR imaging,the lesions were low or isodense on T1WI and hyper-intensity on T2WI in 18 cases,both were high signal intensity on T1WI and T2WI in 6 cases,high signal intensity on T1WI and mixed signal intensity on T2WI in 4 cases.An intracystic nodule having high signal intensity on T1WI,and low or mixed signal intensity on T2WI was observed in 4 cases.On contrast-enhanced MR imaging,enhancement of the cyst's wall was shown in 9 cases.During surgery,the lesions were noted to have a cyst of semisolid consistency,and cystic contents were described from CSF-like clear fluid in 10 cases,jellied-like brown mucoid fluid in 15 cases,caseous-like mucoid fluid in 12 cases,machine oil-like mucoid fluid in 6 cases.At histopathology,a part of cystic fluid included cholesterol crystal and necrotic debris.Cholesterol clefts and hemosiderin pigment,and granuloma were shown by staining with HE in 11 cases.The PAS staining was positive in 16 cases,cystic fluid contained partial mucopolysaccharides and protein.Conclusion Typical Rathke cysts can be dignosised in the preoperative,the findings of CT and MRI are not specific in atypical cysts.The imaging features were different with the fluid component of Rathke cleft cysts..

Objective To translate the English version of the Nursing Profession Self-Efficacy Scale into Chinese,and to test the reliability and validity of the Chinese version. Methods The reliability and validity of the Chinese version of scale was tested among 480 nurses from Tianjin First Central Hospital. Results The revised Chinese version of the Nursing Professional Self-Efficacy Scale contained 19 entries, the Cronbach′s α coefficient was 0.95, the test-retest reliability was 0.91. Conclusions The revised Chinese version of the Nursing Profession Self-Efficacy Scale has acceptable reliability and validity. It can be used to measure the Nursing Profession Self-Efficacy among nurses in China.

Objective To assess the clinical value of MSCT in diagnosing the overcrowding of osteal posterior cranial fossa (PCF) in adults.Methods MSCT images of a cohort of 52 adult patients with foramen magnum osteal malformation confirmed by surgery (diseased group), and 100 healthy adults (control group) were retrospectively reviewed.Images post-processing techniques included multi-planer reformation (MPR) and volume rendering (VR).The posterior cranial fossa volume (PCFV), posterior cranial fossa height (PCFH), clivus length (CL), clivus gradient (CG), supraocciput length (SL), and anteroposterior diameter of the foramen magnum (FMD) were measured on sagittal images in 52 patients and 100 normal adults.Independent-sample student's t test was used to compare the differences between patients and normal adults.Results The results of PCFV, PCFH, CL, SL,FMD and CG, male of control group were (168.2 ±12.3) cm~3, (38.2 ±1.2), (47.1 ±2.8), (41.1 ±1.8), (36.6 ±4.9) mm, (51.5±3.6)°, female of control group were (157.5 ±10.2) cm~3, (36.5 ±1.4), (46.2 ±2.2), (39.7 ±1.3), (35.2 ±3.8), (49.6±3.1)° ;diseased group were (128.7 ±11.7) cm~3, (30.6 ±1.9), (36.2 ±1.4), (37.3 ±0.9), (33.9 ±3.5)mm, (44.5 ±2.8)° .There was significant sex difference in PCFV, PCFH, CL, SL and CG in control group (t =4.70, 6.44, 4.84, 4.43 and 2.81 respectively, P<0.01), but FMD was not significant(t=1.97,P>0.05); the results of PCFV, PCFH, CL, CG and SL were significant different between diseased group and male of control group (t=16.62, 24.04, 25.01, 14.17 and 10.99 respectively,P<0.01) ; the results of PCFV, PCFH, CL, CG and SL were significant different between diseased group and female of control group (t=13.23, 17.80, 27.50, 11.67 and 8.73 respectively,P<0.01) ;but there were no significant differences of FMD between diseased group and control group, both male and female (t=2.96,2.07, P> 0.05).Conclusions The overcrowding of PCF can be accurately measured by MCST.As a routine preoperative examination, MSCT is helpful in the therapeutic selection and the anatomic and pathologic study of PCF.

Objective To explore the technique and the clinical value of Neuroform stents in combination with Guglielmi detachable coil(GDC) to treat wide-necked basilar artery aneurysms.Methods 30 cases with wide-necked basilar artery aneurysms were treated with Neuroform stent combined with GDC.Of 30 wide-necked basilar artery aneurysms,the location of lesions was at basilar tip in 16,basilar trunk in 9 and the beginning of the basilar in 5.Results In 30 cases,total occlusion was achieved in 25 and partial occlusion in 5 patients.The thrombosis within stent occurred in 2 patinets,and remainder of patients recovered well.There were no thromboembolic events in 20 cases followed up for 3~6 months.Twenty-two patients were angiographically followed up for 3 months after the procedure,among them,the aneurysms visualized and the arteriae burdened aneurysms were passed free in densely packed and 3 aneurysms with neck remnant.Conclusion The Neuroform stent is a easy and safe intracranial stent for embo1ization of wide-necked intracranial aneurysms,especially suitable for wide-necked basilar artery aneurysms with severely tortuous intracranial artery.

Objective: To study the effects of genistein on the proliferation and cell cycle progression of human gastric carcinoma cells. Methods: 3H TdR incorporation test was used to investigate the cell proliferation. Flow cytometry was used to analyze the cell cycle arrest. Immunocytochemistry technique and Western blotting were used to observe the cyclin B and P21 waf1/cip1 protein expression. Results: Genistein inhibited the proliferation of tumor cells significantly, arrested cell cycle progression at G 2/M phase, and enhanced cyclin B and P21 waf1/cip1 protein expression in dose dependent manner. Conclusion:Proliferatory inhibition and G 2/M arrest of human gastric carcinoma cells after treated with genistein may be due to increased stability of cyclin B protein and the expression of P21 waf1/cip1 .