0.01). There was a significant difference in TIMP-1,-2 expressions between saccule injury group and control group (P

Medicine, Chinese Traditional ; methods ; trends

Coronary Disease ; diagnosis ; Humans ; Medicine, Chinese Traditional ; methods

Objective:To investigate the relation between the expression of proliferating cell nuclear antigen (PCNA) and prognosis in lung squamous cell.Methods:The expression of PCNA on 51 cases carcinoma tissues were detected by immunohistochemical SP technique. Thet test and F test was used to analyze the difference of expression of PCNA in different pathological grades and clinic stages.Spearman Grade-relation test was used to analyze the relation between PCNA expression and pathological grades.The difference of survival time was compared by Kaplan-Meier curve and Long-rank test in different PCNA expression.Rusults:The position of PCNA expression almostl located in the nuclear,some in cytoplasm.There was difference of PCNA expression in different clinical stages and pathological grades.( P

Objective: To study the significance of DNA content in oral squamous cell carcinoma (SCC).Methods: DNA content and cell cycle of the cells in 18 cases of oral SCC were analized by image cytometry (ICM). Results: The DNA index (DI) and proliferation index (PI) were remarkably heigher in SCC than in normal epithelium ( P

The levels of the antibody response against,classic swine fever (CSF) vaccine were measured by blocking ELISA at different time for pigs vaccinated with CSF vaccine (CSFV group,n= 3),pigs inoculated with porcine eircovirus type 2 (PCV2) and with CSF vaccine two weeks later (when PCV2 viremia was detected by PCR,PCV2/CSFV group,n=3),or inoculated with both CSF vaccine and PCV2 at the same time (CSFV/PCV2 group,n=3),respectively.And distribution of genome or antibodies specific to PCV2 of the PCV2 infection group,PCV2/CSFV group and CSFV/PCV2 group were also detected by indirect ELISA or PCR,respectively.The results showed that experimental infection of PCV2 was successful.In pigs inoculated with both PCV2 and CSF vaccine,the antibody response to CSF vaccine was significantly lower than that of animals treated with CSF vaccine vaccination alone at 52 days postinoculation (DPI).The average titers of antibodies against CSFV in animals of CSFV group were obviously higher than those of the PCV2/CSFV or CSFV/PCV2 group at 49 and 52DPI,respectively.The positive rate of antibodies against CSFV in pigs of CSFV group was 100% in comparison with that of animals inoculated with PCV2 and the vaccine (only 67%).The results suggested that the infection of PCV2 could suppress the antibody response to classical swine fever vaccine.

Aim To explore the ethanol-induced apoptosis effect on hepatocellular carcinoma(HCC) cells and its relationship to the expression of apoptosis associated genes, bax and bcl-2. Methods The cytotoxic effect of 20～ 100 mL/ L ethanol on HCC cell line HCC-9204 was tested by thiazolyl-blue (MTT) assay. Then apoptosis of HCC-9204 cells was induced with 60 mL/ L of ethanol for 6 h. The morphological change, DNA breakage and the change of DNA content of different cell cycles of the apoptotic cells were detected by May-Grunwald Giemsa(MGG) staining, and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay and flow cytometer respectively. The changes of expression level of Bax and Bcl-2 proteins were detected by immunocytochemical staining and image analysis. Results The higher the concentration of ethanol was, the stronger the cytotoxic effect on HCC-9204 cells was. 60 mL/ L of ethanol could lead to obviously morphological apoptotic changes of HCC-9204 cells, and majority of the cells were TUNEL positive by TUNEL labeling assay. Typical apoptotic sub G1 peak was observed by flow cytometer. The level of Bax protein expression increased significantly after induced with 60 mL/ L of ethanol for 6 h, no expression of Bcl 2 were found before and after induced with ethanol. Conclusion Low dose of ethanol can induce apoptosis of HCC-9204 cells obviously, and occurance of the apoptosis is related to the increase of the level of Bax protein expression.

AIM: To conclude the characteristics of flash visual evoke potentials (FVEP), and pattern visual evoke potentials (PVEP) of the healthy school-age children.And to compare the two methods, in order to find the association of them, and to find the impact of sex, age, and the other biological variables.METHODS: A total of 101 healthy children were recruited (age from 5 to 14.4y, mean 8.27y).Each of them was underwent FVEP and PVEP examinations.Then the results were statistically analyzed by SPSS 13.0.RESULTS: The curves of PVEP are simple and stable, while FVEP waveforms are variable.The latency of P100 of females is shorter than males.However there was no significant difference for FVEP in sex control.To compare the parameters between the two hemispheres, the amplitude of P100 of left eyes were higher than the right side.FVEP showed no difference in the two hemispheres either.There was no significant difference for age-dependent decreased in neither PVEP nor FVEP.And in a regression analysis of the FVEP and PVEP, we could not find the inner connection of the two methods.CONCLUSION: Based on our research, there were no significant differences in age level or sex control in the period of school-age children.And there is no inner connection of the two methods.The differences between the PVEP and FVEP results might be due to the origin of these two responses.And these two stimuli should be used in a complementary manner not as alternative examinations.

Objective To eukaryotically exp ress B7.1 extracellular domian and investigate its biological activities. Methods Recombinant expression vector pDisplay/B7.1(V+C) wa s transfected into Hela cells by electroporation method. The expression of B7.1( V+C) was identified by immunohistochemistry and in situ hybridization, respectiv ely. In vitro T lymphocyte activation activity was detected by 3H-TdR incoporation method and the aroused cytotoxicity after mixed culture of pDispla y/B7.1(V+C) transfected Hela cell-T cell was observed by MTT method. R esults B7.1(V+C) was expressed successfully on the surface of Hela c ells. The expressed B7.1(V+C) was able to activate T lymphocytes in the pres ence of anti-CD3 monoclonal antibody. Cytotoxicity could be observed when pDisp lay/B7.1(V+C) transfected Hela cells were mixed and co-cultured with T lymphocy tes. Conclusion Eukaryotically expressed B7.1(V+C) main tains its T lymphocyte costimulatory activity, and B7.1(V+C) can be adopted to construct bifunctional molecules for tumor immunotherapy.

Objective:To investigate the application of photoplethysmogram in analyzing the fingertip pulse amplitude volume (PAV)to evaluate the endothelial function in diagnosing coronary heart disease,and to clarify its relationship with the risk factors of cardiovascular diseases.Methods:Total 409 patients with chest pain accepted coronary angiography (CAG)were selected and diveded into positive group (CAG+)(n=288)and negative group (CAG-) (n = 121)according to angiographic results.Fingertip photoplethysmogram was used to analyze the fingertip PAV by the way of applying endothelial function diagnostic after reactive ischemia,and the relationship between the PAV value and the risk factors of coronary heart diseases was analyzed,and the critical reference value of prediction index of coronary heart disease was determined.The risk factors such as age,gender,serum total cholesterol (TC ), low density lipoprotein (LDL ), high density lipoprotein (HDL ), non-HDL, serum triglyceride (TG),hypertension,diabetes,smoking,family history of coronary heart disease,body mass index (BMI)of the subjects in various groups were analyzed,and the relationship between the risk factors of coronary heart disease and PAV was analyzed by Logistic regression analysis.Results:The PAV of patients in CAG+group was significantly lower than that in CAG-group (P <0.01).The peak point of PAV was<1.37,if PAV<1.37 was used to predict the coronary heart disease,the predictive sensitivity,the specificity,the positive prediction and the negative prediction were 74.65%,44.63%, 76.24%, and 42.52%.The Logistic regression analysis showed that PAV was negatively associated with hypertension,smoking history,TG (OR= 1.476,OR=2.002, OR = 1.844;P < 0.01 ). Conclusion: PAV is associated with coronary heart disease and its risk factors (hypertension,smoking history,TG),and PAV=1.37 can be used as the peak point to predict the coronary heart disease.