OBJECTIVE:To establish a HPLC method for the determination of in vitro drug release of Baicalin liposomes.METHODS:The separation was performed on a C18 column(150mm?4.6mm,5?m)at 25℃;the mobile phase was composed of methanol-water-phosohoric acid(47∶53∶0.2)at a flow rate of 1.0mL?min-1.The ultraviolet wavelength was set at 280nm and the sample size was 10?L.RESULTS:The calibration curve showed good linearity in the range of 1.01～80.80?g?mL-1(r=0.999 8)and the average recovery rate was 100.05%(RSD=0.93%).CONCLUSION:The method is sensitive,simple and accurate,and applicable for the study of in vitro drug release of Baicalin liposomes.

To investigate the common mutation patterns of hereditary nonpolyposis colorectal cancer(HNPCC) genes.MethodsUsing PCR-agarose electrophoresis and PCR-SSCP to screen the hMSH2, hMLH1 genes mutation of 9 HNPCC patients and 4 family members. ResultsAbnormal shifting of PCR productions was identified in 7 patients and 2 HNPCC family members out of 9 different families. ConclusionPCR-agarose and PCR-SSCP are simple, rapid , sensitive, economic and specific methods that can be used to detect HNPCC gene hMSH2, hMLH1 mutations.

After the concept of damage control resuscitation is introduced, in the most severely injured casualties, when the lethal triad of hypothennia, acidosis, and coagulopathy are present, resuscitation prac-tice for damage control usually focuses on rapid reversal of acidosis and prevention of hypothermia, while di-rect treatment of eoagulopathy has been relatively neglected. Recent studies have brought back to light the importance of treating the coagulopathy of trauma at an earlier stage. This review focused on the importance and the right time of treating this disorder, analyzed the methods of addressing the early coagalopathy in damage control resuscitation and gave some advices.

A cochlear implant (CI) is a kind of surgically implanted electronic device that provides a sense of sound to a person who has severe to profound sensorineural hearing impairment. However, studies have shown that the performance in music perception for CI users has not been achieved to an ideal level. It is important to evaluate the ability to perceive music using well designed music perception test materials, in order to improve the quality of life for cochlear implant users. This paper reviewed the studies on assessing music perception ability for adult cochlear implant users from the existing literature.
Cochlear Implantation ; Cochlear Implants ; Humans ; Music ; Pitch Perception

[Aim] Discuss Chen's treatment effect of infants viral myocarditis ayyhythmia. [Methods] He made suit medicine on two cases and analysed the disease'classification and main clinical manifestation and relevant therapy and his empirical formula, according to some factors ,such as the etiology and pathogens, course of disease and so on. [Results] (1)For viral myocarditis early disease caused by tachyarrhythmia, Mr. Chen advocated using the methods to treat which is clearing away heat and toxin and calming the pulse, the prescription used is Qingxinjiedu Decoction;however, those ones with il ness are offered a way of suppiementing qi and nourishing yin and warming the heart yang in order to remove pulse frequency, Chen used the modified YiQiShengMai Decoction for treatment.(2) The professor Chen paid attention to clearing hot and humid and supplementing qi and warming yang to cure slow arrhythmia due to acute viral myocarditis,the modified Huolian Decoction is used for this disease; For the people with a long il ness delayed healing, Chen worked a Decoction by himself which is Wenyangfumai Decoction,so that it could supplement qi and warm yang and invigorate the circulation of blood through pulse; For the slow arrhythmia appeared to sequela period, Chen thought that treatment should be insisted on promoting blood circulation to remove blood stasis and warming and activating the heart yang, the prescription used is Tongmai oyal liquid.[Conclusion]Professor Chen had gained a satisfied effect to treat infants viral myocarditis arrhythmia with the methods of clearing away heat and toxin, supplementing qi and nourishing yin and warming the heart yang and removing pulse frequency from the different stages and types.

Audiology ; methods ; Hearing Tests ; methods ; Humans ; Time Factors

[Objective]To investigate the protective effects of calcitonin on articular cartilage and subchondral bone in experimental osteoarthritis induced by ACLT(anterior cruciate ligament transection) of rats. [Methods]Thirty 12-week-old Sprague Dawley rats were divided randomly into three groups: Sham(n=10),ACLT+CT(n=10) and ACLT+NS(n=10).ACLT+CT group received a daily subcutaneous injection of salmon calcitonin at a dose of 10 IU.kg-1.d-1 for 12 weeks,ACLT+NS group received NS at the same dose.All rats were sacrificed at 12 weeks after operation.The macro-pathological changes of Samples were graded by Mankin's scale.Bone mineral density(BMD) of right distal femoras and femora condyles were measured by dual-energy X-ray absorptiometry scanner.The decalcified right femora condyles were prepared for paraffin sections,stained with safranin-O/fast green and immunohistochemistry for MMP-13.The right proximal tibias were harvested to make undecalcified bone section for detecting the bone histomorphometry of subchondral bone.[Results](1) The Mankin score of the Sham and ACLT+CT groups were significantly lower than that of ACLT+NS group.(2) The BMD and bone mass(BV/TV,Tb.Th) of the subchondral bone in ACLT+NS were significantly higher than those of Sham and ACLT+CT groups.(3)In ACLT+ NS group,expression level of MMP-13 was significantly lower than those of Sham and ACLT+ CT groups.[Conclusion]A daily subcutaneous injection of salmon calcitonin at a dose of 10 IU.kg-1.d-1 of body weight for 12 weeks can inhibit articular cartilage degeneration of rat osteoarthritic joints,inhibitiing sclerosis of and improving the microarchitecture of subchondral bone,coupled with downregulation of MMP-13 in cartilage probably participate in this process.

Objective:To design a tuberculosis(TB) laboratory equipment management system based on Internet of Things (IOT), and put forward the specific plan of IOT technology used in TB laboratory equipment management.Methods: Bases on data acquisition technology, wireless mobile terminal based on wireless LAN technology, radio frequency identification (RFID) technology and computer technology, the Web application system architecture built equipment management system to realize internal information sharing and management of laboratory equipment.Results: The tuberculosis laboratory equipment management system using RFID networking technology and 2-dimensional bar code identification based on recognition, positioning and tracking of equipment, built system architecture by the user login module, device management module, equipment ledger management module and public affairs workflow management module. It has high efficiency, and is convenient for data query, updating and unifying management, information monitoring, real-time dynamic effect of maintenance equipment for positioning and bio safety management.Conclusion:The application of program links TB laboratory equipment management system networking and related technology based on IOT can achieve recognition, positioning and tracking of laboratory equipment, and also can effectively improve the overall level of laboratory equipment management intelligence and laboratory management efficiency.

After the status quo of ESI-covered pharmacology, toxicology and clinical medicine in domestic universities of traditional Chinese medicine was described, the papers and highly-cited papers published by these universities, total citations, citations of each paper were ranked and compared.The related problems and development tendency of different subjects were analyzed with suggestions put forward for the construction of first class subjects in domestic colleges and universities of traditional Chinese medicine.

OBJECTIVE: This study intends to preliminarily discuss the expression and the clinical meaning of serum YKL-40 in anaphylactic rhinitis by comparing the different expressions of serum YKL-40 in types of anaphylactic rhinitis, and to preliminarily discuss possible mechanism of having anaphylactic rhinitis involved with serum YKL-40 by associating it with serum IL-4 and IFN-γ in anaphylactic rhinitis. METHOD: Firstly, each select 20 people in our hospital who have anaphylactic rhinitis respectively according to different levels--mild intermittent, mild persistent, moderate-severe interminttent, moderate-severe persistent as the experimental groups, while 20 healthy people as the nomal control. Secondly, test the levels of serum YKL-40, IL-4 and IFN-γ respectively in each group by the ELISA method. Thirdly, statistically analyze and discuss the collected data. RESULT: (1) There was a rise in the expression of serum YKL-40 between the experimental groups and the nomal control group, which contained a statistical significance (P < 0.05). There was a rise in the expression of serum YKL-40 between the mild persistent group and mild intermittent group, which contained a statistical significance (P < 0.05), as well as between the moderate-severe persistent group and the moderate-severe intermittent group (P < 0.05), while there was no significant difference in expression of serum YKL-40 between the mild intermittent group and the moderate-severe intermittent group, as well as between the mild persistent group and the moderate-severe persistent group (P > 0.05). (2) There was a rise in the expression of serum IL-4 between the experimental groups and the nomal control group, which contained a statistical significance (P < 0.05), while there was no significant difference between each experimental group (P > 0.05). (3) There was a reduction in the expression of serum IL-4 be- tween the experimental groups and the nomal control group, which contained a statistical significance (P < 0.05), while there was no significant difference between each experimental group (P > 0.05). (4) The corelation between serum YKL-40 and IL-4 in anaphylactic rhinitis was positive, while the corelation between serum YKL-40 and IFN-γ was negative. CONCLUSION (1) Allergic rhinitis serum YKL-40 expression was increased. The corelation between serum YKL-40 and IL-4 in anaphylactic rhinitis was positive, while the corelation between serum YKL-40 and IFN-γ was negative,suggesting that YKL-40 may regulate the differentiation of Th cells, Promote Th1 to Th2 immune shift, through this mechanism involved in the pathogenesis of allergic rhinitis. (2) Compared with patients with intermittent allergic rhinitis, persistent allergic rhinitis serum YKL-40 expression was increased, suggesting that YKL-40 may promote the continuous attacks of the symptoms of allergic rhinitis.
Adipokines ; blood ; Case-Control Studies ; Chitinase-3-Like Protein 1 ; Humans ; Interferon-gamma ; blood ; Interleukin-4 ; blood ; Lectins ; blood ; Rhinitis, Allergic ; blood