By ELISA assay, 26 sample of rats (Lao Cai: 14 samples, Hoa Binh: 12 samples) were studies in the year 2002. Serum-positive results in suspective rats of Lao Cai accounted for 28.57%, Hoa Binh 25%. The rate of antihantanvirus antibody was 26.92% in both provinces, Positive serum sample in ELISA technique were verified with mediate immunofluorescein technique giving 14.2% positive in Lao Cai samples. The results were consistent with the epidemiological characteristics of Hantan fever, an endemic of mountain regions
Hantavirus ; Serology ; Rats

In the year 2003, 525 specimens seem to be infected with Dengue fever/Dengue haemorrhagic fever in the Northern Vietnam were sent to the National Institute of Hygiene and Epidemiology, those specimens with IgM, IgG antibodies against Dengue virus was 85 cases (16.2%). In 2003, the number of Dengue fever/Dengue haemorrhagic fever was sporadic distributed in some provinces. Owing to active prevention of Dengue fever/Dengue haemorrhagic fever, so Dengue virus type 2 discovered early in Ha Tay, Thanh Hoa, Nghe An and Tay Nguyen provinces; Dengue virus type 1 in Ha Noi, Nghe An, Tay Nguyen provinces and Dengue virus type 4 in Ha Tinh, Thanh Hoa, and Tay Nguyen provinces
Dengue ; epidemiology ; Serology ; Virology

Hantaan virus is the pathogene of 2 forms of high mortality of hemorrhagic fever with kidney syndrome and lung syndrome. By using the 4 techniques of enzyme addhesion ELISA, immue fluorescemce, and western blot, 61 samply of serum of Rattus Novergicus, in Thanh Hoa, year 2003, ELISA rat serum was (+) and 9/61 were suspected. More over, by immunofluoresceime and western blot, antihantavirus antibody was confirmed on 6/9 samples. These 6 sample from the rats, were studied by WB technique with specific serum SEO, in 2 samples, 51 KDa band was found. Results concluded that in Rattus Novergicus from Thanh Hoa, anti Hantaan virus antibody was detected.
Hantaan virus/epidemiology ; Serology ; Viruses ; Epidemiology

Seven cases of surgically proven sparganosis were serologically tested by means of micro ELISA for their specific IgG antibody levels. For that purpose, crude saline extract of spargana from snake, Natrix tigrina lateralis was prepared and used as antigen. The sparganosis sera were also tested with Paragonimus and Cysticercus antigens to observe the cross reactivity. A total of 71 sera from normal control, ectopic and pulmonary paragonimiasis, clonorchiasis, cysticercosis and Taenia saginata cases were also included. Except for one case of old calcified infection, all of 6 human sparganosis showed higher serum levels of specific IgG antibody when the differential point of positive reaction was set at the absorbance value of 0.25 (the sensitivity being 85.7%). In control and other helminthic infections, all except 3 cases of T. saginata infection showed negative reaction to sparganum antigen (the specificity being 95.7%). None of sparganosis cases showed cross reactivity to Paragonimus and Cysticercus antigens. Undiluted cerebrospinal fluid also showed high levels of antibody when central nervous system was invaded. The serologic diagnosis by means of micro-ELISA could be a useful tool in epidemiological study of human sparganosis in susceptible population, as well as in individual diagnosis.
parasitology-helminth-cestoda ; sparganosis ; sparganum ; Paragonimus ; Cysticercus ; ELISA ; immunology ; serology ; human

To analyse the reason for one case of hemolytic transfusion reaction, antibodies in a patient's serum were identified using panel cells and Le (a-b-) phenotype cells, patient phenotype was identified by using anti-Le(a) and anti-Le(b) blood grouping reagents and the entire coding region of FUT3 gene was amplified by PCR and sequenced directly. The results showed that both IgM anti-Le(a) and anti-Le(b) antibodies were detected in patient's serum. Red cells was typed as Le (a-b-) phenotype and the FUT3 genotype was homozygote for non-functional le(59, 508) alleles. In conclusion, anti-Le(b) antibody can result in hemolytic transfusion reaction, FUT3 gene is homozygous for le(59, 508) allele resulting in Le (a-b-) phenotype.
Adult ; Antibodies ; adverse effects ; immunology ; Blood Grouping and Crossmatching ; Female ; Fucosyltransferases ; genetics ; Genotype ; Hematologic Diseases ; Humans ; Lewis Blood-Group System ; immunology ; Serology ; Transfusion Reaction

BACKGROUND AND OBJECTIVES: In September 2009, Metro Manila was hit by a heavy rainfall typhoon Ketsana inundating several cities of Metro Manila causing an outbreak of leptospirosis. We analyzed the prognostic factors associated with mortality among leptospirosis patients admitted after the typhoon at nine tertiary hospitals from September to November 2009.
METHODS: We reviewed the charts of patients with probable and confirmed leptospirosis. Confirmed leptospirosis was based on any of the following: positive leptospiral culture of blood or urine, single high leptospira microagglutination titer (MAT) of 1:1600 or a fourfold rise in MAT antibody titers or seroconversion. Patients with negative serology or cultures but with history of wading in floodwaters plus any of the following signs and symptons: fever, headache, myalgia, conjunctival suffusion, diarrhea and abdominal pain, jaundice, oliguria and changes in sensorium were considered probable cases.
RESULTS: We analyzed 332 probable and 259 confirmed leptospirosis patients. Mean age was 37.95± 14.09, mostly males (80.2%). Almost all patients (98%) waded in floodwaters. Majority had moderate to severe form of leptospirosis (83%). Acute renal failure was the most common complication (87.1%). Mortality was 11.3% mostly due to pulmonary hemorrhage. On multivariate analysis of confirmed and probable cases, the factors independently associated with mortality were pulmonary hemorrhage (OR 2.75, 95% CI 1.46 to 5.20), severity of the disease (OR 3.85, 95% CI 1.60 to 9.26), thrombocytopenia (OR 3.16, 95% CI 1.22-8.16), duration of illness before admission (OR 0.88, 95% CI 0.78-0.99) and age (OR 1.03, 95% CI 1.00-1.06).
CONCLUSION: Pulmonary hemorrhage remains a poor prognostic factor and strong predictor of mortality among patients with severe leptospirosis. Early consult through heightened awareness of the public and prompt recognition of leptospirosis among clinicians can decrease the risk for progression to complications of leptospirosis and mortality.

 

Human ; Male ; Female ; Adult ; Adolescent ; Hemorrhage ; Mortality ; Serology ; Abdominal Pain ; Acute Kidney Injury ; Cyclonic Storms ; Diarrhea ; Disease Outbreaks ; Fever ; Headache ; Jaundice ; Leptospira ; Leptospirosis ; Multivariate Analysis ; Myalgia ; Oliguria ; Philippines ; Prognosis ; Seroconversion ; Tertiary Care Centers ; Thrombocytopenia