1.Effects of aqueous extract of Hedysarum austrosibiricum on metabolism of oxyen free radicals in subacute aging mice caused by D-galactose.
Taoerdahong HAILIQIAN ; Jin-sen KANG ; Lian SUN
China Journal of Chinese Materia Medica 2007;32(8):729-731
OBJECTIVETo investigate the anti-aging effect of aqueous extract of Hedysarum austrosibiricum cultivated in Xin-jiang.
METHODSubacute aging model in mice was established by D-galactose (D-gal) and activities of SOD and GSH-PX, contents of MDA in brain and liver tissues, activities of MAO in brain tissue and immune indexes were determined.
RESULTAqueous extract of H. austrosibiricum Xinjiang markedly increased the activities of SOD and GSH-PX, significantly decreased contents of MDA in brain and liver tissues. MAO activities in brain tissue were also decreased. It also elevated the spleen and thymus indexes.
CONCLUSIONAqueous extract of H. austrosibiricum might have anti-aging effect, which is implemented by eliminating oxyen free radicals, rising activities
Aging ; drug effects ; metabolism ; Animals ; Antioxidants ; isolation & purification ; pharmacology ; Brain ; drug effects ; metabolism ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Fabaceae ; chemistry ; Female ; Free Radical Scavengers ; isolation & purification ; pharmacology ; Free Radicals ; metabolism ; Galactose ; toxicity ; Glutathione Peroxidase ; metabolism ; Liver ; drug effects ; metabolism ; Malondialdehyde ; metabolism ; Mice ; Monoamine Oxidase ; metabolism ; Plant Extracts ; isolation & purification ; pharmacology ; Plant Roots ; chemistry ; Random Allocation ; Superoxide Dismutase ; metabolism
2.Therapeutic effect of sea buckthorn oil on allergic contact dermatitis in mice
Qi SHEN ; Mei WANG ; sen Jin KANG
Chinese Journal of Comparative Medicine 2017;27(11):50-55
Objective To study the therapeutic effect of sea buckthorn oil on allergic contact dermatitis(ACD) in mice. Methods A total of 60 ICR mice were randomly divided into 6 groups: blank control group, model control group,three groups of high-dose, medium-dose and low-dose sea buckthorn oil(100%, 50%, 25%, ad us. ext.), respectively,and positive control group(compound dexamethasone acetate cream), with 10 mice in each group. The mouse model of ACD was established by sensitization and stimulation with 2,4-dinitrochlorobenzene(DNCB),and injuries in the ear skin of the mice were scored and used as the indicator of successful establishment of the model. The degree of ear swelling and the spleen index of the mice in each group were measured at 48 h after stimulation. Hematoxylin and eosin staining was used to observe the pathological changes in the mouse ear skin. Results Measurement of the degrees of ear swelling showed that the high-dose sea buckthorn oil group(9 mL/kg,ad us. ext.)obviously inhibited the DNCB-induced ear swelling in the mice, with a significant difference compared with the model group(P < 0.01)and non-significant difference compared with the positive control group(P> 0.05). The result of the spleen index assessment showed that sea buckthorn oil at each dose may play an immune-regulatory role by promoting the development of immune organs. The pathological examination showed that epidermis thickening of the ear tissue,epidermal and dermal edema and infiltration of lymphocytes were reduced, dermal vasodilatation and congestion were alleviated, and inflammatory cell infiltration was inhibited in the three groups of different doses of sea buckthorn oil compared with the model control group. Conclusions Sea buckthorn oil has an obvious inhibitory effect on the DNCB-induced allergic contact dermatitis in mice. However, its related mechanisms remain to be further investigated.
3.Intact Canal Wall Mastoidectomy Combined with Balloon Dilation Eustachian Tuboplasty in the Treatment of Middle Ear Cholesterol Granuloma.
Yong-Kang OU ; ; Xue-Yuan ZHANG ; ; Yao-Dong XU ; ; Hao XIONG ; ; Mao-Jin LIANG ;
Chinese Medical Journal 2018;131(6):741-742
Adult
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Cholesterol
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Dilatation
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Ear Canal
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Ear Neoplasms
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surgery
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Ear, Middle
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pathology
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Eustachian Tube
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Female
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Granuloma
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surgery
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Humans
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Male
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Mastoidectomy
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Middle Aged
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Treatment Outcome
4.Breast tumor classification based on serial grayscale ultrasound images.
Zhang HA ; Chuan-Fu LI ; Jin-Ping WANG ; Kang-Yuan ZHOU ; Zhen-Sen YANG
Chinese Journal of Medical Instrumentation 2008;32(3):186-189
This paper proposes an algorithm of evaluating the compression depth, and then to extract four normalized mammary elasticity characteristic parameters with respect to the compression depth. The classification experiments show that these elasticity parameters have a good capability in determining whether the tumor is benign or malignant, and if combined with morphological parameters, the accuracy, sensitivity and specificity can be improved and increased to 95.19%, 98.82% and 92.16%, respectively.
Algorithms
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Breast Neoplasms
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diagnostic imaging
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Elasticity Imaging Techniques
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Female
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Humans
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Sensitivity and Specificity
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Ultrasonography, Mammary
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methods
5.Chest or Abdominal Compression for Prevention of Respiratory Depression in Patients under Propofol Sedation during Gastroscopy
Xiamait AERDAKE ; Jing-ru WEI ; Tian-you LU ; Kang-feng MAI ; Jia-ai LI ; Chao-jin CHEN ; Zi-qing HEI ; Xiao-yun LI
Journal of Sun Yat-sen University(Medical Sciences) 2022;43(4):631-638
ObjectiveTo explore the preventive effects of chest compression, abdominal compression and combined thoracoabdominal compression on propofol-induced respiratory depression during gastroscopy. MethodsA total of 544 patients underwent propofol sedation during gastroscopy in our hospital were randomly divided into 4 groups (n=136 each): Group C (control group), Group T (chest compression group), Group A (abdominal compression group) and T&A (combined thoracoabdominal compression group). Altogether 20 chest, abdominal and combined thoracoabdominal compressions were started respectively on patients in Group T, A and T&A before performing gastroscopy and after unconsciousness at a rate of 30 compressions per minute with a compression depth of 2~3 cm. The incidence of oxygen desaturation (SpO2
6.Role of transthoracic echocardiography in transcatheter closure for atrial septal defect in children.
Guo-ping JIANG ; Jin HE ; Man-li KANG ; Cheng-sen XIA ; Wei-hua ZHU ; Jing-jing YE ; Lei ZHAO ; Xue-hui PENG ; Yu HE ; Xiu-zhen YANG
Journal of Zhejiang University. Medical sciences 2004;33(4):365-367
7.Expression of CD45 in the serum of patients with Crohn's disease.
Liang KANG ; Zu-li YANG ; Lei WANG ; Mei-jin HUANG ; Wei LIU ; Ming-tao LI ; Jian-ping WANG
Journal of Southern Medical University 2009;29(2):259-263
OBJECTIVETo search for differentially expressed proteins in the serum of patients with Crohn's disease.
METHODSSerum protein samples obtained from 4 patients with Crohn's disease and 4 normal adults were cross-labeled with different CyDyes and underwent two-dimensional differential in-gel electrophoresis (2-D DIGE) and imaging analysis. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to identify the differentially expressed proteins.
RESULTS2-D DIGE revealed that the protein on spot 973 was overexpressed by 2.55 folds in the serum of patients with Crohn's disease compared with that in normal adults (P<0.05). The protein was identified as CD45 using mass spectrometry.
CONCLUSIONCD45 overexpression in the serum of patients with Crohn's disease may play a role in the disequilibrium of the immune system.
Amino Acid Sequence ; Case-Control Studies ; Crohn Disease ; blood ; immunology ; Electrophoresis, Gel, Two-Dimensional ; Female ; Humans ; Leukocyte Common Antigens ; blood ; Male ; Molecular Sequence Data ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
8.Association between hypermethylation of Syk gene and clinicopathological characteristics in colorectal cancer patients.
Zu-li YANG ; Lei WANG ; Liang KANG ; Jun-sheng PENG ; Jun XIANG ; Mei-jin HUANG ; Jian-ping WANG
Chinese Journal of Gastrointestinal Surgery 2008;11(5):458-461
OBJECTIVETo investigate the association of the methylation status and expression level of Syk gene with the clinicopathological characteristics in colorectal cancer (CRC) patients.
METHODSMethylation-specific PCR(MSP) and RT-PCR techniques were used to analyze the methylation status and expression level of Syk gene in cancer and normal tissues of 120 CRC patients, meanwhile, association of the methylation status and expression level of Syk gene with the clinicopathological characteristics and the prognosis were studied.
RESULTS(1) Syk gene expression was not found in 48 cancer tissues out of 120 patients and was found in all the normal tissues.The difference was significant. (2) Loss of Syk expression was found in 37 patients with Syk hypermethylation, and in 11 out of 83 patients with Syk nonmethylation. (3) The methylation status of Syk gene was correlated with the lymph node status and the Dukes stage, but not with other clinicopathological parameters. (4) The follow-up data revealed that the 3-year survival of patients with Syk hypermethylation was lower than that of patients without Syk hypermethylation(73.5% vs.95.7%,P=0.007),and postoperative recurrence rate significantly increased in the Syk hypermethylation group (32.4% vs. 8.4%,P=0.02).
CONCLUSIONHypermethylation leads to silence of Syk gene involved in the initiation of colorectal cancer, which increases the infiltration of colorectal cancer cells, postoperative relapse and decreases the postoperative 3-year survival time.
Adult ; Aged ; Aged, 80 and over ; Colorectal Neoplasms ; metabolism ; pathology ; DNA Methylation ; Female ; Humans ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoplasm Staging ; Prognosis ; Receptor Protein-Tyrosine Kinases ; genetics ; metabolism ; Spleen ; metabolism ; Young Adult
9.Serum proteomic variation study in patients with Crohn disease.
Liang KANG ; Zu-Li YANG ; Wei LIU ; Long-Juan ZHANG ; Shao-Jun LIU ; Mei-Jin HUANG ; Ming-Tao LI ; Jian-Ping WANG
Chinese Journal of Gastrointestinal Surgery 2008;11(3):266-269
OBJECTIVETo search differentially expressed proteins in serum of patients with Crohn disease.
METHODSSerum protein samples from 4 patients with Crohn disease and 8 healthy adults were recruited cross-labeled with variant CyDye, and then followed by two-dimensional differential in-gel electrophoresis (2-D DIGE), image analysis, and identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).
RESULTSThe 2-D electrophoresis results were compared between the Crohn disease patients and the healthy adults. The spot 1058 expression in serum of Crohn disease patients increased by 1.68 folds as compared with healthy adults (P<0.05). The protein was identified as haptoglobin by mass spectrometry.
CONCLUSIONUp-regulating expression of haptoglobin in serum of Crohn disease patients may play a role in disequilibrium of immunity system.
Adult ; Blood Proteins ; metabolism ; Case-Control Studies ; Crohn Disease ; blood ; Electrophoresis, Gel, Two-Dimensional ; Haptoglobins ; metabolism ; Humans ; Proteomics ; methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
10.Meta-analysis of randomized controlled trials comparing laparoscopic with open surgery for rectal cancer on oncologic clearance and long-term oncologic outcomes.
Mei-jin HUANG ; Hui PENG ; Hui WANG ; Jing-lin LIANG ; Ben-qiang RAO ; Liang KANG ; Xing-wei ZHANG ; Jian-ping WANG
Chinese Journal of Gastrointestinal Surgery 2011;14(8):606-610
OBJECTIVETo compare oncologic adequacy of resection and long-term oncologic outcomes between laparoscopic-assisted surgery (LS) and open surgery (OS) in the treatment of rectal cancer.
METHODSLiterature searches of electronic databases (PubMed, Embase, Web of Science,and Cochrane Library) and manual searches up to June 30, 2010 were performed to identify RCTs comparing values of oncologic adequacy of resection, recurrence and survival following LS and OS. Fixed and random effects models were used.
RESULTSSix RCTs enrolling 1033 participants (LS group:577 cases, OS group:456 cases)were included in the meta-analysis. Number of lymph node harvested was similar(WMD=-0.38, 95%CI:-1.35-0.58, P=0.43). LS had a slightly higher circumference resection margin(CRM) positive rate with no statistical significance[7.94% vs. 5.37%; risk ratio(RR)=1.13; 95%CI:0.69-1.85, P=0.63]. There was no significant difference between the two groups in local recurrence (RR=0.55; 95%CI:0.22-1.40, P=0.21). The 3-year overall survival [Hazard ratio(HR)=0.76; 95%CI:0.54-1.07, P=0.11] and 3-year disease-free survival(HR=1.16; 95%CI:0.61-2.20, P=0.64) were not significantly different between the two groups.
CONCLUSIONCompared with open surgery, laparoscopic surgery of rectal carcinoma offers similar oncological clearance and long-term oncological outcomes.
Humans ; Laparoscopy ; Laparotomy ; Randomized Controlled Trials as Topic ; Rectal Neoplasms ; surgery ; Treatment Outcome