1.Effects of aqueous extract of Hedysarum austrosibiricum on metabolism of oxyen free radicals in subacute aging mice caused by D-galactose.
Taoerdahong HAILIQIAN ; Jin-sen KANG ; Lian SUN
China Journal of Chinese Materia Medica 2007;32(8):729-731
OBJECTIVETo investigate the anti-aging effect of aqueous extract of Hedysarum austrosibiricum cultivated in Xin-jiang.
METHODSubacute aging model in mice was established by D-galactose (D-gal) and activities of SOD and GSH-PX, contents of MDA in brain and liver tissues, activities of MAO in brain tissue and immune indexes were determined.
RESULTAqueous extract of H. austrosibiricum Xinjiang markedly increased the activities of SOD and GSH-PX, significantly decreased contents of MDA in brain and liver tissues. MAO activities in brain tissue were also decreased. It also elevated the spleen and thymus indexes.
CONCLUSIONAqueous extract of H. austrosibiricum might have anti-aging effect, which is implemented by eliminating oxyen free radicals, rising activities
Aging ; drug effects ; metabolism ; Animals ; Antioxidants ; isolation & purification ; pharmacology ; Brain ; drug effects ; metabolism ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Fabaceae ; chemistry ; Female ; Free Radical Scavengers ; isolation & purification ; pharmacology ; Free Radicals ; metabolism ; Galactose ; toxicity ; Glutathione Peroxidase ; metabolism ; Liver ; drug effects ; metabolism ; Malondialdehyde ; metabolism ; Mice ; Monoamine Oxidase ; metabolism ; Plant Extracts ; isolation & purification ; pharmacology ; Plant Roots ; chemistry ; Random Allocation ; Superoxide Dismutase ; metabolism
2.Therapeutic effect of sea buckthorn oil on allergic contact dermatitis in mice
Qi SHEN ; Mei WANG ; sen Jin KANG
Chinese Journal of Comparative Medicine 2017;27(11):50-55
Objective To study the therapeutic effect of sea buckthorn oil on allergic contact dermatitis(ACD) in mice. Methods A total of 60 ICR mice were randomly divided into 6 groups: blank control group, model control group,three groups of high-dose, medium-dose and low-dose sea buckthorn oil(100%, 50%, 25%, ad us. ext.), respectively,and positive control group(compound dexamethasone acetate cream), with 10 mice in each group. The mouse model of ACD was established by sensitization and stimulation with 2,4-dinitrochlorobenzene(DNCB),and injuries in the ear skin of the mice were scored and used as the indicator of successful establishment of the model. The degree of ear swelling and the spleen index of the mice in each group were measured at 48 h after stimulation. Hematoxylin and eosin staining was used to observe the pathological changes in the mouse ear skin. Results Measurement of the degrees of ear swelling showed that the high-dose sea buckthorn oil group(9 mL/kg,ad us. ext.)obviously inhibited the DNCB-induced ear swelling in the mice, with a significant difference compared with the model group(P < 0.01)and non-significant difference compared with the positive control group(P> 0.05). The result of the spleen index assessment showed that sea buckthorn oil at each dose may play an immune-regulatory role by promoting the development of immune organs. The pathological examination showed that epidermis thickening of the ear tissue,epidermal and dermal edema and infiltration of lymphocytes were reduced, dermal vasodilatation and congestion were alleviated, and inflammatory cell infiltration was inhibited in the three groups of different doses of sea buckthorn oil compared with the model control group. Conclusions Sea buckthorn oil has an obvious inhibitory effect on the DNCB-induced allergic contact dermatitis in mice. However, its related mechanisms remain to be further investigated.
3.Intact Canal Wall Mastoidectomy Combined with Balloon Dilation Eustachian Tuboplasty in the Treatment of Middle Ear Cholesterol Granuloma.
Yong-Kang OU ; ; Xue-Yuan ZHANG ; ; Yao-Dong XU ; ; Hao XIONG ; ; Mao-Jin LIANG ;
Chinese Medical Journal 2018;131(6):741-742
Adult
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Cholesterol
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Dilatation
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Ear Canal
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Ear Neoplasms
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surgery
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Ear, Middle
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pathology
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Eustachian Tube
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Female
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Granuloma
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surgery
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Humans
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Male
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Mastoidectomy
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Middle Aged
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Treatment Outcome
4.Breast tumor classification based on serial grayscale ultrasound images.
Zhang HA ; Chuan-Fu LI ; Jin-Ping WANG ; Kang-Yuan ZHOU ; Zhen-Sen YANG
Chinese Journal of Medical Instrumentation 2008;32(3):186-189
This paper proposes an algorithm of evaluating the compression depth, and then to extract four normalized mammary elasticity characteristic parameters with respect to the compression depth. The classification experiments show that these elasticity parameters have a good capability in determining whether the tumor is benign or malignant, and if combined with morphological parameters, the accuracy, sensitivity and specificity can be improved and increased to 95.19%, 98.82% and 92.16%, respectively.
Algorithms
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Breast Neoplasms
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diagnostic imaging
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Elasticity Imaging Techniques
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Female
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Humans
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Sensitivity and Specificity
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Ultrasonography, Mammary
;
methods
5.Chest or Abdominal Compression for Prevention of Respiratory Depression in Patients under Propofol Sedation during Gastroscopy
Xiamait AERDAKE ; Jing-ru WEI ; Tian-you LU ; Kang-feng MAI ; Jia-ai LI ; Chao-jin CHEN ; Zi-qing HEI ; Xiao-yun LI
Journal of Sun Yat-sen University(Medical Sciences) 2022;43(4):631-638
ObjectiveTo explore the preventive effects of chest compression, abdominal compression and combined thoracoabdominal compression on propofol-induced respiratory depression during gastroscopy. MethodsA total of 544 patients underwent propofol sedation during gastroscopy in our hospital were randomly divided into 4 groups (n=136 each): Group C (control group), Group T (chest compression group), Group A (abdominal compression group) and T&A (combined thoracoabdominal compression group). Altogether 20 chest, abdominal and combined thoracoabdominal compressions were started respectively on patients in Group T, A and T&A before performing gastroscopy and after unconsciousness at a rate of 30 compressions per minute with a compression depth of 2~3 cm. The incidence of oxygen desaturation (SpO2
6.Effects of irbesartan on nitric oxide system in the heart of diabetic rats.
Xiao-xian QIAN ; Yan-ming CHEN ; Wei-kang WU ; Yong LIU ; Bin ZHOU ; Jin-lai LIU ; Lin CHEN
Journal of Southern Medical University 2006;26(9):1359-1362
OBJECTIVETo investigate the effects of irbesartan for heart protection and on heart nitric oxide (NO) system in diabetic rats.
METHODSThirty adult male Wistar rats were randomly divided into three equal groups, namely control group, diabetes group and irbesartan group. Streptozotocin (STZ, 50 mg/kg) was injected to the abdomen to induce diabetes in the rats. After treatment for 12 weeks, the rats were sacrificed and the urine volume, body weight, ratio of heart to body weight, plasma glucose and glycosylated hemoglobin (HbA1c) were measured. NO levels in the serum and myocardium were determined. Inducible nitric oxide synthase (iNOS) expression was determined by immunohistochemistry, and iNOS mRNA detected by RT-PCR.
RESULTSUrine volume, ratio of heart to body weight, plasma glucose, HbA1C, NO levels in the urine, blood and myocardium in diabetic and irbesartan rats were significantly greater than those of normal controls (P<0.05). The ratio of heart to body weight and NO levels of urine, serum and heart tissue in rats of irbesartan group were significantly decreased as compared with those of diabetes rats (P<0.05). Myocardium iNOS mRNA and protein expression decreased significantly in irbesartan group, but not in diabetes group.
CONCLUSIONSThe abnormality in NO and iNOS mRNA expression might be related to diabetic cardiomyopathy. Irbesartan can decrease iNOS mRNA and protein expressions and reduce NO levels in STZ-induced diabetic rats.
Angiotensin II Type 1 Receptor Blockers ; pharmacology ; Animals ; Biphenyl Compounds ; pharmacology ; Diabetes Mellitus, Experimental ; enzymology ; genetics ; metabolism ; Gene Expression Regulation, Enzymologic ; drug effects ; Immunohistochemistry ; Male ; Myocardium ; enzymology ; metabolism ; Nitric Oxide ; blood ; metabolism ; urine ; Nitric Oxide Synthase Type II ; genetics ; metabolism ; RNA, Messenger ; biosynthesis ; genetics ; Random Allocation ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction ; Tetrazoles ; pharmacology
7.Expression of CD45 in the serum of patients with Crohn's disease.
Liang KANG ; Zu-li YANG ; Lei WANG ; Mei-jin HUANG ; Wei LIU ; Ming-tao LI ; Jian-ping WANG
Journal of Southern Medical University 2009;29(2):259-263
OBJECTIVETo search for differentially expressed proteins in the serum of patients with Crohn's disease.
METHODSSerum protein samples obtained from 4 patients with Crohn's disease and 4 normal adults were cross-labeled with different CyDyes and underwent two-dimensional differential in-gel electrophoresis (2-D DIGE) and imaging analysis. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to identify the differentially expressed proteins.
RESULTS2-D DIGE revealed that the protein on spot 973 was overexpressed by 2.55 folds in the serum of patients with Crohn's disease compared with that in normal adults (P<0.05). The protein was identified as CD45 using mass spectrometry.
CONCLUSIONCD45 overexpression in the serum of patients with Crohn's disease may play a role in the disequilibrium of the immune system.
Amino Acid Sequence ; Case-Control Studies ; Crohn Disease ; blood ; immunology ; Electrophoresis, Gel, Two-Dimensional ; Female ; Humans ; Leukocyte Common Antigens ; blood ; Male ; Molecular Sequence Data ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
8.Role of transthoracic echocardiography in transcatheter closure for atrial septal defect in children.
Guo-ping JIANG ; Jin HE ; Man-li KANG ; Cheng-sen XIA ; Wei-hua ZHU ; Jing-jing YE ; Lei ZHAO ; Xue-hui PENG ; Yu HE ; Xiu-zhen YANG
Journal of Zhejiang University. Medical sciences 2004;33(4):365-367
9.Study on independent factors on the prognosis of colorectal carcinoma: TNM stage, tumor budding, perineural invasion, peritumoral-lymphocytic infiltration and urine glucose.
Fang-ying XU ; Jian-kang DONG ; Yi-min ZHU ; Mei-juan QU ; Fen-juan WANG ; Yi-sen JIN ; Guo-ping REN ; Mao-de LAI
Chinese Journal of Epidemiology 2005;26(5):366-369
OBJECTIVETo study the influence of clinical and pathological-morphological parameters on the prognosis of colorectal carcinoma.
METHODSUnivariate and multivariate Cox proportional hazard model were used to study the influence of clinical and pathological-morphological factors on the prognosis in 226 colorectal carcinoma cases.
RESULTSUsing univariate analysis, data showed that the factors significantly related to disease prognosis would include: the depth of direct spread, vessel invasion, perineural invasion, tumor budding, peritumoral-lymphocytic infiltration, Crohn-like reaction, number of positive lymph nodes, distant metastasis, TNM stage and urine glucose. Multivariate Cox proportional hazard model showed that six factors were identified to be associated with higher relative-risk (RR), including: older age, advanced TNM stage, more severe budding, perineural invasion, less peritumoral-lymphocytic infiltration and urine glucose.
CONCLUSIONAge, TNM stage, tumor budding, perineural invasive, peritumoral-lymphocytic infiltration and urine glucose were independent predictors to the prognosis of colorectal carcinoma.
Aged ; China ; epidemiology ; Colorectal Neoplasms ; diagnosis ; epidemiology ; pathology ; Female ; Glycosuria ; Humans ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoplasm Invasiveness ; Neoplasm Staging ; Prognosis ; Proportional Hazards Models ; Regression Analysis ; Retrospective Studies ; Risk Factors
10.Influence of clinical and pathomorphological parameters on prognosis in colon carcinoma and rectal carcinoma.
Fang-ying XU ; Mei-juan DI ; Jian-kang DONG ; Feng-juan WANG ; Yi-sen JIN ; Yi-min ZHU ; Mao-de LAI
Journal of Zhejiang University. Medical sciences 2006;35(3):303-310
OBJECTIVETo investigate the effects of clinical and pathomorphological parameters on the prognosis of colon carcinoma and rectal carcinoma.
METHODSUnivariate and multivariate COX proportional hazard models were used to study the effects of the clinical and pathomorphological factors on the prognosis in 101 cases of colon carcinoma, 219 of rectal carcinoma and 137 of rectal carcinoma under curative resections.
RESULTBy using univariate analysis, we identified that lymph node metastasis and distant metastasis were the common prognostic factors for both colon carcinoma and rectal carcinoma. Smoking, deep infiltration, chemotherapy and serum albumin concentration were the uncertain prognostic factors for colon carcinoma. Signet-ring cell carcinoma, larger tumor size (>6 cm), deep infiltration, lack of radical surgery, and advanced TNM stage were the exclusive adverse prognostic factors for rectal carcinoma. Further studies showed that the adverse prognostic factors for the rectal carcinoma under curative resection included deep infiltration, lymph node metastasis, vessel invasion, less of peritumoral lymphocyte infiltration, lack of Crohn's like reactivity, high level of tumor budding, advanced TNM stage and positive urine glucose. By using multivariate analysis based on a COX proportional hazard model, it was identified that smoking, lymph node metastasis and serum albumin concentration were independent prognostic factors for colon carcinoma; advanced TNM stage, distant metastasis and palliative surgery for rectal carcinoma; and vessel invasion, lymph node metastasis and urine glucose for rectal carcinoma under curative resections.
CONCLUSIONThe various clinical and pathomorphological parameters show different prognostic value for colon carcinoma, rectal carcinoma and rectal carcinoma under curative resections.
Adult ; Aged ; Carcinoma, Signet Ring Cell ; pathology ; surgery ; Colonic Neoplasms ; pathology ; surgery ; Female ; Humans ; Lymphatic Metastasis ; Male ; Middle Aged ; Prognosis ; Proportional Hazards Models ; Rectal Neoplasms ; pathology ; surgery