1.Clinical study on gut barrier dysfunction and bacterial translocation in patients following gastrointestinal surgery
Zhi QIAO ; Rong LI ; Zhanliang LI
Medical Journal of Chinese People's Liberation Army 2001;0(10):-
Objective To investigate gut barrier dysfunction and bacterial translocation (BT) from gastrointestinal tract in postoperative patients using polymerase chain reaction (PCR) and plasma D-lactate measurement, and to study the relationship between BT and infections complications. Methods 34 patients undergoing selective gastrointestinal operations were observed. Blood samples were collected prior to and 2, 24, 48 h after surgery for bacterial culture, plasma D-lactate, and LPS measurement. PCR was used to detect microbial DNA. PCR was performed after DNA extraction, with target ?-lactosidase gene of E. coli and 16S rRNA gene of most pathogenic bacteria. All the patients were observed for 30 days for monitoring infectious complications. Results No bacterial DNA was detected before surgery, but it was positive in 7 patients (20.6%) after operation. Bacterial DNA was discovered in 38.9% (7/18) of SIRS (systemic inflammatory response syndrome) patients, no bacterial DNA was detected in the 16 non-SIRS patients (P
2.Hemorheology of patients with silicosis.
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(9):563-565
Adult
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Aged
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Erythrocyte Indices
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Hemorheology
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Humans
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Male
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Middle Aged
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Silicosis
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blood
3.Clinical effects of ganglioside on delayed encephalopathy after acute carbon monoxide poisoning
Zhirong ZHAO ; Jiandi ZHANG ; Aiping WU ; Rong WANG ; Zhi LI
Clinical Medicine of China 2012;28(8):864-866
Objective To observe the clinical effects of hyperbaric oxygen combined large dose ganglioside on delayed encephalopathy after acute carbon monoxide poisoning(DEACMP).Methods A totol of 72 patients of DEACMP were randomly divided into the observation group(37)and the control group(35).The patients of control group were given the routine treatment with hyperbaric oxygen.In the treatment group patients were given large doses of gangliosides 100 mg,1 times a day,for a period of 20 to 30 days additionally to the routine treatment of hyperbaric oxygen.All patients were examined before and after treatment in EEG examination (EEG),mini-mental state examination(MMSE).Results The abnormal rate of EEG was 37.83%(14/37) in the control group,which was significantly lower than that of 65.71%(23/35) in the treatment group((x2 =5.60,P < 0.05).In the treatment group,the MMSE was(15.45±2.93) and(23.70±2.13) before and after treatment,respectively; in the control group,the MMSE was(14.88±2.84) and(20.33±2.09) before and after treatment.The MMSE was significantly impoved after treatment in both group(t =3.18 and 2.91,Ps <0.05).Furthermore,the MMSE impoved more significantly in the treatment group compared to the control group (t =6.28,P < 0.05).The effective rate of treatment group was 86.48%(32/37),which was significantly higher than that of 51.43%(20/35) in the control group(x2 =7.72,P < 0.01).Conclusion Application of large dose ganglioside treatment had significant clinical effects in delayed encephalopathy after acute car-bon monoxide poisoning,which is better than the effect of routine hyperbaric oxygen treatment.
4.Prokaryotic expression and polyclonal antiserum preparation of Schistosoma japonicum Mago nashi like protein
Zhi-rong, ZHAO ; Li, LEI ; Ji-jia, SHEN
Chinese Journal of Endemiology 2008;27(2):158-161
Objective To express Schistosoma japonicum Mago nashi(SjMago)gene,and prepare its specific polyclonal antibody.Methods SjMago gene was amplified by PCR from Schistosomulum cDNA library and subcloned into pET28a(+)vector,its recombinant proteins were expressed with IPTG.Rabbits were immunized with the polyacrylamide gel particles containing the recombinant proteins for polyclonal antibody preparation,the sera were detected for antibody specificity by Western blot and titer by ELISA assay.Results SjMago prokaryotic expression plasmid was successfully recombined and the target proteins was induced by IPTG in a molecular weight of 17 X 103,the high titer(1∶40 960)polyclonal antibody was isolated from the immunized rabbit,specific rotein band was detected by Western blot.Conclusion SjMago protein has been successfully expressed and its specific polyantibody is prepared,which lays the foundation for further study.
5.A Cross-sectional Study on Diabetes Mellitus in Rural Population in Kunming
Mao-Rong ZHANG ; Lan SHEN ; Zhi-Kun LI
Chinese Journal of Prevention and Control of Chronic Diseases 2006;0(06):-
Objective To study the epidemiological characteristics of diabetes mellitus and to provide effective prevention and intervention methods in rural area in Kunming.Methods A cross-sectional study was conducted among 3 440 permanent residents aged 20~74 years in kunming rural districts.Results The general prevalence rate of diabetes was 4.01% and the standardized rate was 2.90%.The standardized prevalent rate for male(3.55%) was higher than that for female(2.61%),and the prevalence rates varied among different groups of rural inhabitants.Conclusion It is necessary to take effective methods to prevent and control diabetes in rural area.
6.Progress in Alzheimer's disease treated with insulin sensitizer
Zhi-Min SHAO ; Rong WANG ; Shu-Li SHENG ;
Chinese Journal of Endocrinology and Metabolism 2001;0(05):-
In Alzheimer's disease there is obvious evidence of insulin resistance in the brain. Thiazolidinediones,a kind of insulin sensitizer,not only improves insulin sensitivity,but also decreases inflammation,promotes release and clearance of?-amyloid protein,all are beneficial to the improvement of memory.
8.Effect of retinoic acid treatment on lung injury in hyperoxia-exposed newborn rats.
Zhi-hui RONG ; Li-wen CHANG ; Qian-shen ZHANG
Chinese Journal of Pediatrics 2003;41(4):299-300
Animals
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Animals, Newborn
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Hyperoxia
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physiopathology
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Lung
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drug effects
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pathology
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Oxygen
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pharmacology
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Rats
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Rats, Sprague-Dawley
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Tretinoin
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pharmacology
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therapeutic use
9.Protracted low-dose temozolomide combined with concomitant whole brain radiotherapy for brain metastases from non-small cell lung cancer.
Zhi-fang LIU ; Hui-qin LI ; Rong-jie TAO
Chinese Journal of Oncology 2011;33(10):792-793
Adult
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Aged
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Agranulocytosis
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chemically induced
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Antineoplastic Agents, Alkylating
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administration & dosage
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adverse effects
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therapeutic use
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Brain Neoplasms
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secondary
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therapy
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Carcinoma, Non-Small-Cell Lung
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pathology
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Chemoradiotherapy
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Dacarbazine
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administration & dosage
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adverse effects
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analogs & derivatives
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therapeutic use
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Disease-Free Survival
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Dose-Response Relationship, Drug
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Female
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Humans
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Lung Neoplasms
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pathology
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Male
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Middle Aged
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Remission Induction
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Survival Rate
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Vomiting
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chemically induced