ObjectiveTo study the effect of ultrasound transdermal drug delivery combining with semiconductor laser on chronic pelvic inflammation disease(CPID).Methods80 CPID patients were divided into two groups randomly: 40 cases for treating group who accepted ultrasound transdermal drug delivery and semiconductor laser. 40 cases for the control group who accepted ultrasound transdermal drug delivery only. After 30 days of treating, a Condition-Score was assessed. ResultsIn the treating group, 18 cured, 19 remarkable effective, 3 effective, which was significant improved compared with that in the control group, 6 cases cured, 21 cases remarkably effect,10 cases effective, 3 case ineffective(P<0.01).ConclusionUltrasound transdermal drug delivery combined with semiconductor laser is more effective on chronic pelvic inflammation disease.

Gestational diabetes mellitus is detrimental to the health of both the mother and the fetus,and therefore greater attention is being paid to the earlier diagnosis and treatment of the condition.This article offers an overview of the research progress in the predictive factors of gestational diabetes mellitus.

The infant with macrosomia has been the focus of attention and controversy for some time, and fetal macrosomia affects neonatal and maternal morbidity and mortality. This review summarized the etiological factors of fetal macrosomia.

This thesis discusses the innovation of pharmaceutical education by analyzing its new trends.The author also puts forward some methods to accelerate the innovation in pharmaceutical education.

Acute retinal necrosis syndrome (ARNS) is a group of eye syndrome.Acute uveitis, retinal artery occlusive vasculitis, fused necrotic retinitis and late stage of retinal detachment is the main clinical manifestation.A part of patients may be associated with increased intraocular pressure.The etiology and pathogenesis is still not clear completely and most people think that may be related to the virus infection, which mainly to reflected to be herpes simplex virus (HSV), varicella zoster virus (VZV), EB virus and giant cell virus (CMV) infection.Its diagnosis mainly depends on clinical manifestation, examination and etiological examination.Acute retinal necrosis syndrome is urgent and develops quickly, and it is lack of specific clinical symptoms in early times.By the way, it enjoys high misdiagnosis rate and poor prognosis.It is hard to cure, therefore, it is an important reason for the blindness.Once diagnosed, treatment should be adopted by carrying local and systemic antiviral, preventive laser photocoagulation in time.At the same time, it is essential that vitreous body resection combine with silicone oil tamponade treatment when necessary.The study shows that the effective measures of early treatment will be able to prevent disease progression and improve visual acuity.Therefore, early diagnosis and treatment of acute retinal necrosis syndrome is very important.In this paper, combination of the literature on the diagnosis and treatment of acute retinal necrosis syndrome were reviewed.

Objective To assess the operation time of laparoscopic operation for acute cholecystitis by Meta analysis.Methods The methods of computer literature retrieval and manual search were used to collect the research on the timing of laparoscopic surgery for acute cholecystitis in recent years,and the random effects model was used to conduct the Meta analyze.The quality of the literature was evaluated,and then the RevMan5.0.21 software was used to calculate the RR value and 95%CI of the operation time,bleeding volume and postoperative complications.Results A total of 13 articles were included in the Meta analysis,9 in Chinese,4 in English,2535 for a total number of cases,1401 in the early operation group,1134 in the delayed operation group,and 10 papers for low risk of bias,3 papers for bias risk.Meta analysis of the random effects model showed that the operation time of the early operation group was significantly shorter than that of the delayed operation group(P=0.00,RR=-20.25,95%CI=-24.97~15.33),and the intraoperative blood loss was also significantly reduced(P=0.00,RR=-12.58,95%CI=-29.36~5.78),then the postoperative complications were also significantly reduced(P=0.00,RR=0.63,95%CI=0.47~0.84).Conclusion The use of laparoscopic operation in the treatment of acute cholecystitis in patients with 72h could not only shorten the operation time and reduce the amount of bleeding,but also had a significant effect on reducing postoperative complications.

Objective To observe the clinical efficacy of Bu Shen Tiao Qi(supplementing the kidney and regulating qi) needling in treating perimenopausal syndrome.Method Fifty patients were randomized into two groups, 25 cases each. The treatment group was intervened by Bu Shen Tiao Qineedling combined with electroacupuncture, while the control group was by shallow needling plus sham electroacupuncture. Kupperman Index (KI) wasobserved respectively before treatment, after 2-week and 4-week treatment, and in the 4-week follow-up study. The differences of scores and improvements of the 13 symptoms listed in KI were calculated.Result The total effective rate was 92.0% in the treatment group versus 44.0% in the control group; in the treatment group, the KI scores after 2-week treatment and in the 4-week follow-up study were significantly different from that before treatment (P<0.05), and a more significant difference was found after 4-week treatment (P<0.01); the between-group differences were statistically significant in comparing KI (P<0.05).Conclusion Bu Shen Tiao Qineedling can produce a satisfactory efficacy in treating perimenopausal syndrome.

Objective To study the changes in gene expression profiles of dendritic cells(DCs) during their maturation processes using cDNA microarray.Method DCs were generated from human peripheral blood monocytes induced by GM-CSF and IL-4,and lipopolysaccharide(LPS) was used to induce the maturation of DCs.After inducing for 48h,cells were collected to extract the general RNA.Genes expressions of mature DCs(maDC) and immature DCs(imDC) were analyzed using cDNA microarray,and immature DCs without induction served as control.Results DCs expressed highly surface markers of mature DCs after LPS induction.The studied data showed that among those 165 target genes,a total of 50 genes(30.3%) exhibited differential levels of expression in LPS-induced mature DCs.Thirty-five genes were up-regulated and fifteen genes were down-regulated.Maturation-dependent up-regulation,defined by a differential expression ratio of ≥2,included twelve cytokine and chemokine genes,ten antigen uptake and presentation genes and thirteen signal transduction molecule genes.Reciprocally,maturation-dependent down-regulation,defined by a differential expression ratio of ≤0.5,occurred with two cytokine,five antigen uptake and presentation genes and eight signal transduction molecule genes.Semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) was employed to further confirm the results of cDNA microarray.Conclusion It is suggested that LPS has significant influence on many genes of DCs in their maturing processes.Those changed genes include cytokines,chemokines and their receptors,antigen uptake and presentation molecules and signal transduction molecules.Analysis on these differentially expressed genes is helpful in understanding the maturing processes of DCs.

Objective To construct the eukaryotic expression plasmids of Mycobacterium tuberculosis ESAT-6 antigen and to investigate its immunogenicity in mice. Methods The sequence encoding ESAT-6 was amplified by PCR from Mycobacterium tuberculosis H37Rv genomic DNA. Then, the amplified fragment was sub-cloned into pVAC expression vector. The eukaryotic expression plasmid pVAC-ESAT-6 was transfected into Vero E6 cells with liposome. The mRNA expression of pVAC-esat-6 in Vero E6 cells was detected by RT-PCR and its expression product was analyzed by Western-blot. The plasmid pVAC-esat-6 was introduced into the mice by gene gun injection. The specific antibody to ESAT-6 and the level of IFN-? in supernatant of spleno-lymphocyte cultures were detected by ELISA methods. Results The recombinant plasmid pVAC-esat-6 was successfully constructed, and the expression of ESAT-6 was also detected in vitro. After vaccinated three times, the mice produced specific antibody and the level of IFN-? in supernatant of spleno-lymphocyte cultures in group of pVAC-esat-6 was significantly higher than group of vector alone (P

Objective To investigate the correlations of the levels of serum total cholesterol (TC),high density lipoprotein(HDL),and low density lipoprotein (LDL) with tumor node metastasis (TNM) staging and Lauren's classification in gastric cancer.Methods The blood lipid levels of 157 cases of gastric cancers,80 cases of benign gastric diseases,and 80 cases of normal subjects were detected by the Hitachi 7170A automatic biochemical instrument,and these values were statistically compared with SPSS 19.0.Results The contents of serum TC,HDL,and LDL in the group of gastric cancers were lower than those in the groups of normal subjects and benign gastric diseases (P ＜ 0.05).The contents of serum HDL levels were decreased with the increase of the TNM stage in patients with gastric cancer,and were all lower than the benign gastric diseases and the health control group (P ＜ 0.05).No statistically significant correlation was found in the levels of serum TC,HDL,and LDL in Lauren's histological types of gastric cancers (P ＜0.05).Conclusions Abnormal lipid metabolism had already appeared in gastric cancer,and the decreased serum HDL levels was correlated with TNM stage of gastric cancer.Detection of the changes of lipid levels in patients might be beneficial to the screening and prognosis of gastric cancer.