1.Exploratory Development of 3DSS Three-dimensional Scanning System on Unilateral Orbital Defect Restoration
Xuepeng TANG ; Lijun QIU ; Chunyan ZHANG
Chinese Medical Equipment Journal 1989;0(03):-
Objective To discuss the accuracy of 3DSS three-dimensional scanning system, which was used to reconstruct the 3D digital model of unilateral orbital defect patients. Methods By using 3DSS spectrometer on the case of patients with unilateral orbital defect, the related scan dates of facial structure were collected, and the reconstructed digital models were processed wax pattern by SLS prototyping machine. Results The digital solid model of three-dimensional reconstruction was smooth and complete, and border over the natural. The designed resin prosthesis can be inosculated with the orbital defect tightly and symmetrically. Conclusion To adopt the system can be successfully completed unilateral orbital defect restoration design and production, and ultimately satisfied the clinical requirement of prosthesis production.
2.Acute hibernating myocardium in isolated rat heart
Yuangang QIU ; Junhua ZHANG ; Chaoshu TANG
Chinese Journal of Interventional Cardiology 1996;0(01):-
We set up an animal model of acute hibernating myocardium isolated rat heart. which were perfused with human red blood cell-enhanced modified K-H buffer,were used. During ischemia,coronary flow were reduced to 20%. The results show: At 30 minutes of ischemia,left ventircular peak systolic pressure,+dp/dt max and - dp/dt max were decreased to 44% ,33% and 26% (P
3.Glycosylated hemoglobin A1c for the diagnosis of diabetes mellitus: a meta-analysis
Songtao TANG ; Qiu ZHANG ; Changjiang WANG ; Haiqin TANG ; Taixiang WU
Chinese Journal of Internal Medicine 2013;(1):21-25
Objective To assess the diagnostic value of glycosylated hemoglobin A1c (HbA1c) ≥6.5% for diabetes in Chinese adults with oral glucose tolerance test(OGTT) as the reference standard.Methods Major databases were searched to get all diagnostic tests with HbA1c ≥ 6.5% for diabetes in Chinese adults.QUADAS items were used to evaluate the quality of the eligible studies.Meta-disc software was used to perform comprehensive quantitative assessment for all included studies and summary ROC (SROC) curve were drawn.Results A total of 11 studies were included.The outcomes of the diagnostic value with HbA1c ≥6.5% were as the following:pooled sensitivity 0.62 (95% CI:0.60-0.64),pooled specificity 0.96 (95% CI:0.95-0.96),diagnostic odds ratio (DOR) 40.25 (95% CI:20.79-77.95) and AUCSROC 0.7702 (sx =0.0636).Conclusions The diagnostic specificity is pretty high for the diagnostic test with HbA1c ≥6.5%,while sensitivity is low.Combination of HbA1c and glucose tests is needed to reduce the missed diagnosis rate.
4.Bioinformatic analysis of antibody repertoire development in response to influenza vaccination
Lu ZHANG ; Qingchen ZHANG ; Tianyi QIU ; Zhiwei CAO ; Kailin TANG
Chinese Journal of Microbiology and Immunology 2017;37(2):133-139
Objective To analyze the immunogenomic characteristics of antibody repertoire in re-sponse to influenza vaccine in order to provide a theoretical basis for further development of antibody. Meth-ods Based on a time-series immunoglobulin heavy chain ( IGH) repertoire sequencing dataset, we analyzed the immunogenomic characteristics of antibody repertoire in response to trivalent influenza vaccine ( TIV ) from three aspects which included the features in complementarity-determining region 3 ( CDR3 ) , antibody mutation and VDJ usage. Results The frequency of antibody mutation increased significantly upon vaccina-tion. Analysis of the CDR3 region indicated that polar and aromatic amino acids had a higher preference. The length of CDR3 region in naive B cells followed a normal distribution, while specific CDR3 sequences with 15 to 18 amino acids in length occupied a dominant position after vaccination. In addition, the VDJ us-age altered obviously and IGHV3-7-derived antibody had a significant response to the vaccine. Response in-tensity reached the peak on day 7 and gradually weakened over time. Conclusion Antibody repertoire evolves dynamically to express specific antibody upon vaccination and the characteristics of immune responses at sequence level could be used to evaluate their effectiveness.
5.Studies on antioxidation of extract from Ruppia rostellata koch
Jiamin ZHANG ; Xin QIU ; Pingyuan HUANG ; Weichao TANG
Chinese Journal of Marine Drugs 1994;0(02):-
Extract from Ruppia rostellata Koch was divided into three parts: petroleum ether part, CH2Cl2 and water-soluble part. Each part of them was studied upon it's antioxidation. The results indicated that the active compounds were only in the water-soluble part. In soybean oil, the antioxidation of water-soluble part was stronger than that of BHA and V. E. , In lard, the antioxidation of water-solubile part was a little weaker than that of BHA and V.E.
6.Effect of acute hypervolemic hemodilution on expression of plasma bactericidal/permeability-increasing protein in patients undergoing total hip replacement
Qifeng TANG ; Yuhua QIU ; Zhongyun WANG ; Hongxing ZHANG ; Yanning QIAN
Clinical Medicine of China 2009;25(7):701-703
Objective To study the effect of acute hypervolemic hemodilution on expression of plasma bac-tericidaL/permeability-increasing protein (BPI) in patients undergoing total hip replacement. Methods Twenty ASA Ⅰ-Ⅱ patients undergoing elective total hip replacement were randomly divided into two groups (n=10 for thesia. The blood loss,blood transfusion and the time of operation were recorded. Venous blood samples were taken before anesthesia (T0) ,at the begining of operation (T1) ,30 min after operation (T2) ,and at the end of operation (T3) for determination of plasma bactericidal/permeability-increasing protein. Results The blood loss and the blood transfusion in HES group were significantly lower than that of LR group[blood loss: (560±90)ml vs (810±110) ml and blood transfusion: (200±100) ml vs (600±200) ml,t=5.562 and 5.657,P<0.001]. The plasma BPI concentrations in HES group were significantly increased at T2~T3 as compared to baseline value at T0 [(8.9±1.6)μg/L,(13.4±1.2)μg/L and (4.9±1.2)μg/L,P<0.05]. The plasma BPI concentrations in LR group were significantly increased at T2~T3 as compared to baseline value at T0 [(7.3±1.2)μg/L,(9.9±0.8) μg/L and (5.0±1.1)μg/L,P<0.05],but were lower than those in HES group (t=2.530 and 7.674,P=0.021 and 0.001 ). Conclusion Acute hypervolemic hemodilution with 200/0.5 hydroxyethyl starch can reduce blood transfusion during total hip replacement operation and also can increase the BPI level which would beneficial for the immunological function.
7.Analysis of the clinical effect of she-saiko-to combined with silymarin on liver fibrosis due to chronic hepafitis B
Bing QIU ; Peiyi ZHANG ; Xiaoyan TANG ; Qinghua LI ; Dongfu LI
Chinese Journal of Primary Medicine and Pharmacy 2010;17(1):38-39
Objective To evaluate the therapeutic effect of sho-saiko-to silymarin on liver fibrosis due to chronic hepatitis B.Methods 90 patients with liver fibrosis due to chronic hepatitis B virus(HBV) were randomly divided into therapy group and control group.50 patients in therapy group were given sho-saoko-to and silymarin;While 40 patients in control group were given silymarin only,they were all treated for 6 months.The changes were observed including liver function,serum liver fibrosis and virology indexes before and after treatment in two groups.Resuits Indexes of liver function and serum liver fibrosis in therapy group were improved obviously(P<0.05 or P<0.01),while better clearance to HBV is comparable to control group(P<0.05).Conclusion The treatment with sho-saiko-to combined silymarin on liver fibrosis due to chronic hepatitis B had explicit clinical effect.
8.Study on the formation of amyloid fibrils by self-assembly of an artificially designed peptide GAV-6.
Jie ZHANG ; Chengkang TANG ; Yongzhu CHEN ; Zhihua XING ; Feng QIU
Journal of Biomedical Engineering 2014;31(3):686-690
Amyloid fibrils belong to a category of abnormal aggregations of natural proteins, which are closely related to many human diseases. Recently, some critical peptide sequences have been extensively studied for clarifying the molecular mechanism of natural proteins to form amyloid fibrils. In the present study, we designed a short peptide GGAAVV (GAV-6) composed of hydrophobic amino acids glycine (G), alanine (A) and valine (V) and studied its ability to form amyloid fibrils. As characterized by atomic force microscopy (AFM) and dynamic light scattering (DLS), the peptide could self-assemble into smooth nanofibers without branches. Congo red staining/binding and thioflavin-T (ThT) binding experiments show that the nanofibers formed by GAV-6 shared identical properties with typical amyloid fibrils. These results show that the designed peptide GAV-6 could self-assemble into typical amyloid fibrils, which might make it a useful model molecule to clarify the mechanism for the formation of amyloid fibrils in the future.
Amino Acid Sequence
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Amyloid
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chemistry
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Humans
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Microscopy, Atomic Force
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Models, Molecular
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Nanofibers
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chemistry
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Peptides
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chemistry
9.The Clinical Study on Dilatation Technique with Double Balloon in the Treatment of Cardia Achalasia
Peilin ZHOU ; Dalong TANG ; Yaogang ZHANG ; Chenggong QIU ; Tinghua WANG
Journal of Practical Radiology 1996;0(04):-
Objective To evaluate the curative effect and value of dilatation technique with double balloon in the treatment of cardia achalasia.Methods 52 patients with cardia achalasia were treated by the dilatation technique with double balloon.Results The effective rate was 100% in short term while.In long term,the effective rate was 96% and 100% with one time dilatation and double time dilatations respectively.Conclusion The dilatation technique with double balloon is a safe,effective and easy operated method in the treatment of cardia achalasia.
10.Discrimination Between Natural Caffeine and Synthetic Caffeine
Wei ZHANG ; Mingzhu TANG ; Anying QIU ; Wei WU
China Pharmacy 2001;0(12):-
OBJECTIVE:To establish a method to distinguish between synthetic caffeine and natural caffeine METHODS:To identify the related substances obtained from synthetic caffeine and natural caffeine by HPLC,TLC,fluorescence spectrophotometry and X-ray powder diffraction RESULTS:Synthetic caffeine and natural caffeine can be identified by testing the related substances in them CONCLUSION:Using the described method,synthetic caffeine and natural caffeine can be discriminated accurately