Adult ; Biopsy, Needle ; Diagnosis, Differential ; Humans ; Kidney ; pathology ; Kidney Diseases ; pathology ; therapy ; Male ; Nephritis, Interstitial ; pathology ; Renal Dialysis ; Sarcoidosis ; pathology ; therapy ; Tuberculosis, Renal ; pathology

Coronary artery disease (CAD) is one of the leading causes of death. Safflower attracts great attention owing to its anti-ischemia/reperfusion injury effect. Ninety-three patients with CAD were included and randomized into safflower treatment group, PCI group and control group. Low-dose dobutamine stress echocardiography (DSE) was performed to measure end-systolic volume (ESV), end-diastolic volume (EDV), left ventricular ejection fraction (LVEF) and wall motion score index (WMSI) to determine the recovery of hibernating myocardium and cardiac function in all patients before treatment and after 3-month follow-up. The study was to investigate the effects of safflower on hibernating myocardial revascularization and cardiac function. It was found that LVEF was significantly improved, while the ESV and WMSI were significantly reduced after 2-week treatment in safflower and PCI treatment groups. No significant differences were found between safflower and PCI treatment groups in ESV, EDV, WMSI and LVEF after treatment Safflower injection effectively improved hibernating myocardial function.
Aged ; Carthamus tinctorius ; chemistry ; Coronary Artery Disease ; drug therapy ; physiopathology ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Heart ; drug effects ; physiopathology ; Humans ; Male ; Middle Aged ; Myocardial Revascularization ; Myocardial Stunning ; drug therapy ; physiopathology ; surgery ; Recovery of Function

Objective To explore the clinical effect of low molecular weight heparin calcium combined with psychological intervention in the treatment of recurrent spontaneous abortion. Methods 60 patients were treated with recurrent miscarriage after treatment in our hospital from October 2016 to June 2017, and these patients were randomly divided into two groups: control group and observation group.Each group of 30 cases. First of all, the two groups of patients were treated with low molecular weight heparin calcium, and then the control group of patients to choose the most commonly used care methods for nursing operations,the observation group of patients choose to use more targeted psychological intervention care for nursing work.The clinical effects of the two groups were observed and compared. Results There was no significant difference between the two groups. The adverse reaction rate was 10.00% in the observation group and the control group The adverse reaction rate was 26.67%. The adverse reaction rate of the observation group was significantly lower than that of the control group.The difference between the two groups was statistically significant (P<0.05). Conclusion Recurrence of recurrent miscarriage after treatment of patients with more targeted psychological intervention in nursing care,compared to conventional care methods better,while effectively reducing the low molecular weight heparin calcium treatment of recurrent miscarriage of adverse reactions.The incidence of psychological intervention in the care of the clinical way is a worthy of use and to promote the treatment.

Objective To investigate the characteristics of methadone maintenance treatment(MMT)clients, and to explore their retention rate and associated factors in Taizhou, Zhejiang Province. Methods Heroin or opiate addicts who started MMT between 2006 and 2014 in study area were included. Data including demographics, history of drug use and treatment information was extracted from the National MMT Data Management System and descriptive analysis were conducted. Multiple logistic regression models were used to explore factors associated with retention rate. Results In total, 2 262 MMT clients were included for analysis. Most of them were male(87.53%), single, divorced or widowed(55.75%), and had an education of junior middle school or below(88.68%). Almost all of them (98.32%)were heroin addicts, and the age at first drug use ranged from 9 years to 54 years, with average age of(25.06±6.34)years. A total of 1 123(49.65%)clients had a history of inject drug use and 81(3.58%)clients had ever shared needles with others. The average duration on MMT was 5.02±2.01 years, and the average methadone dosage during treatment was (34.49 ±22.69)mL. The MMT retention rate was 29.2% (661/2 262). In multivariable analysis, districts such as Huangyan District, Yuhuan County and Linhai City, aged≥40 years and average methadone dosage>20 mL during treatment were independently and positively associated with retention rate. Conclusion Most of MMT clients in Taizhou are male, unmarried, and had low education level. The retention rate is low in Taizhou but older patents and those receive high methadone dosage have a relatively higher retention rate.

OBJECTIVETo investigate the expressions of leptin and leptin receptor in hepatocellular carcinoma (HCC) and explore the clinicopathological significance.

METHODSThe expressions of leptin and leptin receptor were examined by immunohistochemistry in 81 HCC patients undergoing curative tumor resection. The correlations between the expression of two biomarkers and the clinicopathological factors were analyzed.

RESULTSThe overexpression rate of leptin and leptin receptor in HCC was 56.8% and 35.8%, respectively. No significant correlation was observed between their overexpression (r=0.236, P=0.034). Leptin receptor overexpression was significantly correlated to the tumor size and TNM stage (P<0.05), but not to age, body mass index, α-fetoprotein, hepatitis B surface antigen status, tumor grade, vascular invasion, or liver cirrhosis (P≥0.05). Leptin overexpression showed no significant correlations to the above clinicopathological factors (P≥0.05).

CONCLUSIONLeptin receptor overexpression may have an inhibitory effect on hepatocellular carcinoma. The expression status of leptin receptor decides the action of leptin and leptin receptor after their binding.

Carcinoma, Hepatocellular ; metabolism ; pathology ; Female ; Humans ; Leptin ; metabolism ; Liver Neoplasms ; metabolism ; pathology ; Male ; Middle Aged ; Neoplasm Staging ; Receptors, Leptin ; metabolism

OBJECTIVETo explore the prognostic values of Raf-1 kinase (Raf-1), phosphorylated mitogen extracellular kinase 1 (pMEK1), and phosphorylated extracellular signal-regulated protein kinase 1/2(pERK1/2) in hepatocellular carcinoma (HCC) patients.

METHODSWe assessed the expressions of Raf-1, pMEK1, and pERK1/2 in HCC using immunohistochemical techniques. The relationships between the expressions of Raf-1, pMEK1, and pERK1/2 and the prognosis were explored.

RESULTSThe over-expression rates of Raf-1, pMEK1, and pERK1/2 in HCC were 38.3%, 46.7%, and 38.3%, respectively. The over-expressions of Raf-1, pMEK1, and pERK1/2 were positively correlated with each other (P>0.05), but had no significant correlation with sex, age, α-fetoprotein, hepatitis B surface antigen status, the TNM stage, size,differentiation and vascular invasion of tumor, and liver cirrhosis (P>0.05). Univariate survival analysis and COX proportional hazard regression model showed that Raf-1 over-expression was an independent prognostic factor of poor survival (P<0.05).

CONCLUSIONRaf-1 over-expression is an independent marker for the patients of HCC, which may provide new clue in the future targeted therapy.

Adult ; Aged ; Carcinoma, Hepatocellular ; diagnosis ; enzymology ; Extracellular Signal-Regulated MAP Kinases ; metabolism ; Female ; Humans ; Liver Neoplasms ; diagnosis ; enzymology ; MAP Kinase Kinase 1 ; metabolism ; Male ; Middle Aged ; Phosphorylation ; Prognosis ; Proto-Oncogene Proteins c-raf ; metabolism

Objective To investigate the efficacy of evidence-based nursing for prevention of pressure sores in senile orthopedics patients. Methods There were 326 senile orthopedics patients received evidencebased nursing in the observation group, and 268 senile orthopedics patients received usual care belonged to control group. To calculate and compare the incidence rate of pressure sores of the two groups. Results The incidence rate of pressure sores in observation group was 0.7% but 4. 2% in control group, the difference between the two groups had statistical significance (P<0. 05). Conclusions Evidence-based nursing can change clinical nurses'habits and behaviors which based on experience. Nursing practice will be promoted, and senile patient's pressure sores will be prevented effectively through using scientific evidence-based nursing theory to guide clinic

Objective:To explore the clinical characteristics and risk factors of cytomegalovirus(CMV)and Epstein-Barr virus(EBV)co-reactivation after allogeneic hematopoietic stem cell transplantation(allo-HSCT)and its influence on prognosis.Methods:The clinical data of 222 patients who received allo-HSCT from January 2015 to December 2020 were collected,and the patients were divided into groups according to the occurrence of CMV and EBV infection.Kaplan-Meier method was used for survival analysis,and Cox proportional hazard regression model was used to analyze the risk factors of co-reactivation of CMV and EBV.Results:After allo-HSCT,there were 30 patients with co-reactivation of CMV and EBV(CMV++EBV+group),101 patients with CMV viremia(CMV+group),149 patients with EBV viremia(EBV+group),and 28 patients with CMV and EBV inactivation(CMV-+EBV-group).Compared with the other groups,the incidence of acute graft-versus-host disease(aGVHD)and hemorrhagic cystitis(HC)was higher in CMV++EBV+groups(53.3％vs 42.6％,36.9％,17.9％,P＜0.001;36.7％vs 32.7％,22.8％,10.7％,P=0.042).The incidence of post-transplant lymphoproliferative disease(PTLD)in CMV++EBV+group was similar to CMV+group and EBV+group(3.3％vs 3.0％,3.4％,P=0.811).Univariate and multivariate analysis showed that the persistent time of CMV and EBV after transplantation were independent risk factors for co-reactivation of CMV and EBV.Compared with the other groups,the 2-year overall survival(OS)rate and 2-year disease-free survival(DFS)rate of patients inCMV++EBV+group were lower(46.7％vs 74.9％,83.4％,71.4％,P＜0.001;46.7％vs 70.9％,79.5％,69.9％,P=0.002),and 2-year non-recurrence mortality(NRM)was higher(48.2％vs 22％,13.6％,18.7％,P＜0.001).Conclusion:The persistent time of CMV and EBV after transplantation are independent risk factors for patients with co-reactivation of CMV and EBV.Patients with co-reactivation of CMV and EBV had lower OS and DFS rate and higher NRM,suggesting that the clinical prognosis of the patients are worse.

OBJECTIVE: To study the protective effect of vitamin A on residual pancreatic β cell function in children with type 1 diabetes mellitus (T1DM) and its mechanism. METHODS: A total of 46 children with T1DM (with a course of disease of 0.5-1 year) were randomly divided into an intervention group and a non-intervention group (n=23 each). The children in both groups were given insulin treatment, and those in the intervention group were also given vitamin A at a daily dose of 1 500-2 000 IU. A total of 25 healthy children were enrolled as the control group. The daily dose of insulin was calculated for the children with T1DM, and the serum levels of glycosylated hemoglobin (HbA1C), stimulated C-peptide, vitamin A, and interleukin-17 (IL-17) were measured before intervention and 3 months after intervention. RESULTS: Before vitamin A intervention, the intervention group and the non-intervention group had a significantly lower serum level of vitamin A and a significantly higher level of IL-17 than the control group (P<0.01). After 3 months of intervention, the intervention group had significantly lower serum IL-17 level and insulin dose and a significantly higher level of stimulated C-peptide than the non-intervention group (P<0.05). CONCLUSIONS Vitamin A may protect residual pancreatic β cell function, possibly by improving the abnormal secretion of IL-17 in children with T1DM.
Blood Glucose ; C-Peptide ; Diabetes Mellitus, Type 1 ; Glycated Hemoglobin A ; Humans ; Infant ; Insulin ; Insulin-Secreting Cells ; Vitamin A

OBJECTIVETo study the gene characterization of enterovirus 71 (EV71) virus strains isolated from clinical specimens of children with hand-foot-and-mouth disease (HFMD) in Zhejiang province.

METHODSVirus were isolated from clinical samples including stool, throat swab and vesicle from patients with HFMD. The EV71 isolates were identified by microneutralization assay and reverse transcriptase PCR (RT-PCR) with specific primer pair for VP1 genes of EV71. Complete VP1 gene sequences (891 nucleotides) for recent 6 EV71 isolates were determined and compared with that of A, B, C genotype reference EV71 strains and 11 EV71 China isolates available from GeneBank by homogeneity and phylogenetic tree analyses.

RESULTS9 strains of EV were isolated from 14 clinical specimens. Data from microneutralization and RT-PCR results indicated that all the strains belong to EV71. The nucleotide and amino acid homogeneity of these 6 Zhejiang strains with the representative isolates of A and B genotypes were 82.9%-85.5% and 94.9%-98.0% respectively; with the representative isolates of C were 89.2%-94.1% and 97.0%-99.0% respectively. There were 91.0%-92.2%, 90.2%-90.3%, 89.2%-89.5%, 96.7%-96.9% nucleotide, homology with representative strains of C1, C2, C3,C4 subgenotypes of EV71. The nucleotide homogeneity of these 6 EV71 isolated strains with 9 previously isolated Chinese strains appeared to be 93.8%-97.1%. These 6 EV71 isolated strains were within genotype C subgenogroup C4 in the phylogenetic tree.

CONCLUSIONThe recently identified EV71 isolates in Zhejiang province belonged to subgenogroup C4.

Child ; China ; Enterovirus ; classification ; genetics ; isolation & purification ; Genes, Viral ; Genotype ; Hand, Foot and Mouth Disease ; virology ; Humans ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid